Abstract:
:Detection of psychrotrophic strains (those able to grow at or below 7 degreesC) of the Bacillus cereus group (Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides) in food products is at present extremely slow with conventional microbiology. This is due to an inability to discriminate these cold-adapted strains from their mesophilic counterparts (those able to grow only above 7 degreesC) by means other than growth at low temperature, which takes 5 to 10 days for detection. Here we report the development of a single PCR assay that, using major cold shock protein-specific primers and appropriate annealing temperatures, is capable of both rapidly identifying bacteria of the B. cereus group and discriminating between psychrotrophic and mesophilic strains. It is intended that this development help to more accurately predict the shelf life of refrigerated pasteurized food and dairy products and to reduce the incidence of food poisoning by psychrotrophic strains of the B. cereus group.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Francis KP,Mayr R,von Stetten F,Stewart GS,Scherer Sdoi
10.1128/AEM.64.9.3525-3529.1998subject
Has Abstractpub_date
1998-09-01 00:00:00pages
3525-9issue
9eissn
0099-2240issn
1098-5336journal_volume
64pub_type
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