Multiplex PCR method for detection of three Aeromonas enterotoxin genes.

abstract：:For simultaneous identification of members of the betaproteobacterial order "Rhodocyclales" in environmental samples, a 16S rRNA gene-targeted oligonucleotide microarray (RHC-PhyloChip) consisting of 79 probes was developed. Probe design was based on phylogenetic analysis of available 16S rRNA sequences from all cultu...

journal_title：Applied and environmental microbiology

authors： Loy A,Schulz C,Lücker S,Schöpfer-Wendels A,Stoecker K,Baranyi C,Lehner A,Wagner M

更新日期：2005-03-01 00:00:00

abstract：:Lowering the pH in bacterium-based succinate fermentation is considered a feasible approach to reduce total production costs. Newly isolated Enterobacter aerogenes strain AJ110637, a rapid carbon source assimilator under weakly acidic (pH 5.0) conditions, was selected as a platform for succinate production. Our previo...

journal_title：Applied and environmental microbiology

authors： Tajima Y,Yamamoto Y,Fukui K,Nishio Y,Hashiguchi K,Usuda Y,Sode K

更新日期：2015-02-01 00:00:00

abstract：:NAD-linked alcohol dehydrogenase activity was detected in cell-free crude extracts from various propane-grown bacteria. Two NAD-linked alcohol dehydrogenases, one which preferred primary alcohols (alcohol dehydrogenase I) and another which preferred secondary alcohols (alcohol dehydrogenase II), were found in propane-...

journal_title：Applied and environmental microbiology

authors： Hou CT,Patel RN,Laskin AI,Barist I,Barnabe N

更新日期：1983-07-01 00:00:00

abstract：:Desulfitobacterium hafniense strain TCE1 is capable of metabolically reducing tetra- and trichloroethenes by organohalide respiration. A previous study revealed that the pce gene cluster responsible for this process is located on an active composite transposon, Tn-Dha1. In the present work, we investigated the effects...

journal_title：Applied and environmental microbiology

authors： Duret A,Holliger C,Maillard J

更新日期：2012-09-01 00:00:00

abstract：:It has long been assumed that prolonged holding of environmental samples at the ambient air temperature prior to bacteriological analysis is detrimental to isolation and detection of Vibrio cholerae, the causative agent of pandemic cholera. The present study was aimed at understanding the effect of transporting enviro...

journal_title：Applied and environmental microbiology

authors： Alam M,Sadique A,Nur-A-Hasan,Bhuiyan NA,Nair GB,Siddique AK,Sack DA,Ahsan S,Huq A,Sack RB,Colwell RR

更新日期：2006-03-01 00:00:00

abstract：:Antibody against nivalenol tetraacetate (tetra-Ac-NIV) was prepared by immunization of rabbits with triacetyl-15-pimelate-NIV conjugated to bovine serum albumin. By using tritiated tetra-Ac-NIV as the test ligand, antibody titers were demonstrated as early as 4 weeks after immunization. Useful antibody for radioimmuno...

journal_title：Applied and environmental microbiology

authors： Wang CR,Chu FS

更新日期：1991-04-01 00:00:00

abstract：:The exopolysaccharides (EPSs) produced by some bacteria are potential growth substrates for other bacteria in soil. We used stable-isotope probing (SIP) to identify aerobic soil bacteria that assimilated the cellulose produced by Gluconacetobacter xylinus or the EPS produced by Beijerinckia indica. The latter is a het...

journal_title：Applied and environmental microbiology

authors： Wang X,Sharp CE,Jones GM,Grasby SE,Brady AL,Dunfield PF

更新日期：2015-07-01 00:00:00

abstract：:Vibrio tubiashii is a recently reemerging pathogen of larval bivalve mollusks, causing both toxigenic and invasive disease. Marine Vibrio spp. produce an array of extracellular products as potential pathogenicity factors. Culture supernatants of V. tubiashii have been shown to be toxic to oyster larvae and were report...

journal_title：Applied and environmental microbiology

authors： Hasegawa H,Lind EJ,Boin MA,Häse CC

更新日期：2008-07-01 00:00:00

abstract：:In this study, we examine bacterial attachment and survival on a titanium (Ti) cathode coated with various carbon nanomaterials (CNM): pristine carbon nanotubes (CNT), oxidized carbon nanotubes (O-CNT), oxidized-annealed carbon nanotubes (OA-CNT), carbon black (CB), and reduced graphene oxide (rGO). The carbon nanomat...

journal_title：Applied and environmental microbiology

authors： Zhang Q,Nghiem J,Silverberg GJ,Vecitis CD

更新日期：2015-07-01 00:00:00

abstract：:Extracellular DNA (eDNA) is a biofilm component that contributes to the formation and structural stability of biofilms. Streptococcus mutans, a major cariogenic bacterium, induces eDNA-dependent biofilm formation under specific conditions. Since cell death can result in the release and accumulation of DNA, the dead ce...

journal_title：Applied and environmental microbiology

authors： Nagasawa R,Yamamoto T,Utada AS,Nomura N,Obana N

更新日期：2020-11-10 00:00:00

abstract：:Desulfitobacterium hafniense strain PCP-1 reductively dechlorinates pentachlorophenol (PCP) to 3-chlorophenol and a variety of halogenated aromatic compounds at the ortho, meta, and para positions. Several reductive dehalogenases (RDases) are thought to be involved in this cascade of dehalogenation. We partially purif...

journal_title：Applied and environmental microbiology

authors： Bisaillon A,Beaudet R,Lépine F,Déziel E,Villemur R

更新日期：2010-11-01 00:00:00

abstract：:In Vibrio vulnificus, virulence for eels is associated with serovar E strains. In this study, we investigated some biological properties of purified lipopolysaccharides (LPSs) from serovar E and non-serovar E strains. Purified LPSs retained their O-polysaccharidic side chains and did not show any differences that coul...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Collado RM,Oliver JD,Amaro C

更新日期：1999-02-01 00:00:00

abstract：:Twelve strains of different Bacteroides species were tested for their efficiency of detection of bacteriophages from sewage. The host range of several isolated phages was investigated. The results indicated that there was a high degree of strain specificity. Then, by using Bacteroides fragilis HSP 40 as the host, whic...

journal_title：Applied and environmental microbiology

authors： Tartera C,Jofre J

更新日期：1987-07-01 00:00:00

abstract：:Most species of the diazotrophic Proteobacteria Azoarcus spp. occur in association with grass roots, while A. tolulyticus and A. evansii are soil bacteria not associated with a plant host. To facilitate species identification and strain comparison, we developed a protocol for PCR-generated genomic fingerprints, using ...

journal_title：Applied and environmental microbiology

authors： Hurek T,Wagner B,Reinhold-Hurek B

更新日期：1997-11-01 00:00:00

abstract：:Marker rescue is an important molecular technique that enables sequential gene deletions. The Cre-loxP recombination system has been used for marker gene rescue in various organisms, including aspergilli. However, this system requires many time-consuming steps, including construction of a Cre expression plasmid, intro...

journal_title：Applied and environmental microbiology

authors： Mizutani O,Masaki K,Gomi K,Iefuji H

更新日期：2012-06-01 00:00:00

abstract：:Environmental studies of the human-pathogenic bacterium Campylobacter jejuni have focused on linking distributions with potential sources. However, in aquatic ecosystems, the abundance of C. jejuni may also be regulated by predation. We examine the potential for grazing by the freshwater planktonic crustacean Daphnia ...

journal_title：Applied and environmental microbiology

authors： Schallenberg M,Bremer PJ,Henkel S,Launhardt A,Burns CW

更新日期：2005-09-01 00:00:00

abstract：:A spontaneous rpsL mutant of Thermus thermophilus was isolated in a search for new selection markers for this organism. This new allele, named rpsL1, encodes a K47R/K57E double mutant S12 ribosomal protein that confers a streptomycin-dependent (SD) phenotype to T. thermophilus. Models built on the available three-dime...

journal_title：Applied and environmental microbiology

authors： Blas-Galindo E,Cava F,López-Viñas E,Mendieta J,Berenguer J

更新日期：2007-08-01 00:00:00

abstract：:Maximum yields of amylase were produced by the thermophilic actinomycete Thermomonospora viridis in a modified Simpson and McCoy medium containing 1.5% corn starch and 0.5% mycological peptone with an initial pH 7.0. Best yields of amylase were obtained after incubation for 48 h, when the pH of the medium had risen to...

journal_title：Applied and environmental microbiology

authors： Upton ME,Fogarty WM

更新日期：1977-01-01 00:00:00

abstract：:Evaluation of the fate and transport of biological warfare (BW) agents in landfills requires the development of specific and sensitive detection assays. The objective of the current study was to develop and validate SYBR green quantitative real-time PCR (Q-PCR) assays for the specific detection and quantification of s...

journal_title：Applied and environmental microbiology

authors： Saikaly PE,Barlaz MA,de Los Reyes FL 3rd

更新日期：2007-10-01 00:00:00

abstract：:Race 1 isolates of Fusarium oxysporum f. sp. lycopersici (FOL) are characterized by the presence of AVR1 in their genomes. The product of this gene, Avr1, triggers resistance in tomato cultivars carrying resistance gene I In FOL race 2 and race 3 isolates, AVR1 is absent, and hence they are virulent on tomato cultivar...

journal_title：Applied and environmental microbiology

authors： Biju VC,Fokkens L,Houterman PM,Rep M,Cornelissen BJC

更新日期：2017-02-01 00:00:00

abstract：:Biocontrol agents generally do not perform well enough under field conditions to compete with chemical fungicides. We determined whether transgenic strain SJ3-4 of Trichoderma atroviride, which expresses the Aspergillus niger glucose oxidase-encoding gene, goxA, under a homologous chitinase (nag1) promoter had increas...

journal_title：Applied and environmental microbiology

authors： Brunner K,Zeilinger S,Ciliento R,Woo SL,Lorito M,Kubicek CP,Mach RL

更新日期：2005-07-01 00:00:00

abstract：:Lysostaphin (Lst) is a potent bacteriolytic enzyme that kills Staphylococcus aureus, a common bacterial pathogen of humans and animals. With high activity against both planktonic cells and biofilms, Lst has the potential to be used in industrial products, such as commercial cleansers, for decontamination. However, Lst...

journal_title：Applied and environmental microbiology

authors： Wu X,Kwon SJ,Kim D,Zha J,Mora-Pale M,Dordick JS

更新日期：2018-07-02 00:00:00

abstract：:Six strains of Clostridium thermocellum isolated from various environments were characterized as to growth rate, production of reducing sugars, ethanol, and acetic acid from cellulose, base composition of DNA, and the abilities to adapt to ethanol and to grow at 45 degrees C. Five of the six new isolates produced 7 to...

journal_title：Applied and environmental microbiology

authors： Bender J,Vatcharapijarn Y,Jeffries TW

更新日期：1985-03-01 00:00:00

abstract：:The diffusive penetration of fluorescently tagged macromolecular solutes into model oral biofilms was visualized by time-lapse microscopy. All of the solutes tested, including dextrans, proteases, green fluorescent protein, and immunoglobulin G, accessed the interior of cell clusters 100 to 200 microm in diameter with...

journal_title：Applied and environmental microbiology

authors： Takenaka S,Pitts B,Trivedi HM,Stewart PS

更新日期：2009-03-01 00:00:00

abstract：:The effects of pulsed electric field (PEF) treatment and processing factors on the inactivation kinetics of Listeria innocua NCTC 11289 were investigated by using a pilot plant PEF unit with a flow rate of 200 liters/h. The electric field strength, pulse length, number of pulses, and inlet temperature were the most si...

journal_title：Applied and environmental microbiology

authors： Wouters PC,Dutreux N,Smelt JP,Lelieveld HL

更新日期：1999-12-01 00:00:00

abstract：:The concentrations of molybdenum (Mo) and 25 other metals were measured in groundwater samples from 80 wells on the Oak Ridge Reservation (ORR) (Oak Ridge, TN), many of which are contaminated with nitrate, as well as uranium and various other metals. The concentrations of nitrate and uranium were in the ranges of 0.1 ...

journal_title：Applied and environmental microbiology

authors： Thorgersen MP,Lancaster WA,Vaccaro BJ,Poole FL,Rocha AM,Mehlhorn T,Pettenato A,Ray J,Waters RJ,Melnyk RA,Chakraborty R,Hazen TC,Deutschbauer AM,Arkin AP,Adams MW

更新日期：2015-08-01 00:00:00

abstract：:By using cryo-scanning electron microscopy and quantification with lectin-conjugated probes, we have detected the production of exopolysaccharides (EPS) in Bifidobacterium animalis subsp. lactis in the presence of bile. In addition, the expression of gtf01207, which codifies a putative priming glycosyltransferase invo...

journal_title：Applied and environmental microbiology

authors： Ruas-Madiedo P,Gueimonde M,Arigoni F,de los Reyes-Gavilán CG,Margolles A

更新日期：2009-02-01 00:00:00

abstract：:Superoxide dismutase (SOD) activity was determined during the growth cycle of unheated and heat-injured cells of Staphylococcus aureus MF-31. SOd activity levels dropped in unheated cells during the lag phase, increased during logarithmic phase, and became constant in the stationary phase. Cells which were sublethally...

journal_title：Applied and environmental microbiology

authors： Bucker ER,Martin SE

更新日期：1981-03-01 00:00:00

abstract：:The objective of this study was to determine the effect of high pressure (HP) on the inactivation of microbial contaminants in Cheddar cheese (Escherichia coli K-12, Staphylococcus aureus ATCC 6538, and Penicillium roqueforti IMI 297987). Initially, cheese slurries inoculated with E. coli, S. aureus, and P. roqueforti...

journal_title：Applied and environmental microbiology

authors： O'Reilly CE,O'Connor PM,Kelly AL,Beresford TP,Murphy PM

更新日期：2000-11-01 00:00:00

abstract：:Injury induced in Escherichia coli cells by chlorination was studied from a physiological standpoint. Predictable and reproducible injury was found to occur rapidly in 0.5 mg of chlorine per liter and was reversible under nonselective conditions. There was an extended lag period in the growth of chlorinated cells not ...

journal_title：Applied and environmental microbiology

authors： Camper AK,McFeters GA

更新日期：1979-03-01 00:00:00