Massive fungal contamination in animal care facilities traced to bedding supply.

abstract：:We determined the effects of grain size and nutritional conditions on the penetration rate and metabolic activity of Escherichia coli strains in anaerobic, nutrient-saturated chambers packed with different sizes of glass beads (diameters, 116 to 767 mum) under static conditions. The chambers had nearly equal porositie...

journal_title：Applied and environmental microbiology

authors： Sharma PK,McInerney MJ

更新日期：1994-05-01 00:00:00

abstract：:Cytophaga hutchinsonii is a widely distributed cellulolytic bacterium in the phylum Bacteroidetes. It can digest crystalline cellulose rapidly without free cellulases or cellulosomes. The mechanism of its cellulose utilization remains a mystery. We developed an efficient method based on a linear DNA double-crossover a...

journal_title：Applied and environmental microbiology

authors： Wang Y,Wang Z,Cao J,Guan Z,Lu X

更新日期：2014-10-01 00:00:00

abstract：:Highly methylated, long-chain celluloses strongly inhibited cellulose degradation by several species of cellulolytic bacteria of ruminal origin. Specifically, the inhibitory effects of methylcellulose on the growth of Ruminococcus flavefaciens FD1 were concentration dependent, with complete inhibition at 0.1% (wt/vol)...

journal_title：Applied and environmental microbiology

authors： Rasmussen MA,Hespell RB,White BA,Bothast RJ

更新日期：1988-04-01 00:00:00

abstract：:The ability of Listeria monocytogenes to tolerate salt stress is of particular importance, as this pathogen is often exposed to such environments during both food processing and food preservation. In order to understand the survival mechanisms of L. monocytogenes, an initial approach using two-dimensional polyacrylami...

journal_title：Applied and environmental microbiology

authors： Duché O,Trémoulet F,Glaser P,Labadie J

更新日期：2002-04-01 00:00:00

abstract：:Regulation of the expression of the two major chitinase genes, ech42 (encoding the CHIT42 endochitinase) and nag1 (encoding the CHIT73 N-acetyl-beta-D-glucosaminidase), of the chitinolytic system of the mycoparasitic biocontrol fungus Trichoderma atroviride (= Trichoderma harzianum P1) was investigated by using a repo...

journal_title：Applied and environmental microbiology

authors： Mach RL,Peterbauer CK,Payer K,Jaksits S,Woo SL,Zeilinger S,Kullnig CM,Lorito M,Kubicek CP

更新日期：1999-05-01 00:00:00

abstract：UNLABELLED:By combining genomics and isotope imaging analysis using high-resolution secondary ion mass spectrometry (NanoSIMS), we examined the function and evolution of Bacteroidales ectosymbionts of the protist Barbulanympha from the hindguts of the wood-eating cockroach Cryptocercus punctulatus In particular, we inv...

journal_title：Applied and environmental microbiology

authors： Tai V,Carpenter KJ,Weber PK,Nalepa CA,Perlman SJ,Keeling PJ

更新日期：2016-07-15 00:00:00

abstract：:A stable bacterial community absorbed and transformed the herbicide metolachlor [2-chloro-N-(2-ethyl- 6-methylphenyl)-N-(2-methoxy-1-methylethyl)-acetamide] from a liquid medium. About 80% of the added ring-[U-C]metolachlor (50 mug/ml) disappeared from the medium and accumulated inside the cells. The ratio of cellular...

journal_title：Applied and environmental microbiology

authors： Liu SY,Zheng Z,Zhang R,Bollag JM

更新日期：1989-03-01 00:00:00

abstract：:A monoclonal antibody (BA11) has been produced against Clostridium botulinum type A neurotoxin by the fusion of myeloma cells (P3 NS1/1-Ag4-1) with spleen cells from BALB/c mice immunized with botulinum type A neurotoxoid. The antibody bound specifically to botulinum type A neurotoxin, showing no cross-reactivity with...

journal_title：Applied and environmental microbiology

authors： Shone C,Wilton-Smith P,Appleton N,Hambleton P,Modi N,Gatley S,Melling J

更新日期：1985-07-01 00:00:00

abstract：:O island 48 (OI-48) of Escherichia coli consists of three functional gene clusters that encode urease, tellurite resistance (Te(r)), and putative adhesins Iha and AIDA-1. The functions of these clusters in enterohemorrhagic E. coli (EHEC) O157:H7 infection are unknown. Deletion mutants for these three regions were con...

journal_title：Applied and environmental microbiology

authors： Yin X,Wheatcroft R,Chambers JR,Liu B,Zhu J,Gyles CL

更新日期：2009-09-01 00:00:00

abstract：:Six laboratories actively involved in water virology research participated in a methods evaluation study, conducted under the auspices of the American Society for Testing and Materials Committee on Viruses in the Aquatic Environment, Task Force on Drinking Water. Each participant was asked to examine the Viradel (viru...

journal_title：Applied and environmental microbiology

authors： Melnick JL,Safferman R,Rao VC,Goyal S,Berg G,Dahling DR,Wright BA,Akin E,Stetler R,Sorber C

更新日期：1984-01-01 00:00:00

abstract：:Thirty years of intensive research have significantly contributed to our understanding of Helicobacter pylori biology and pathogenesis. However, the lack of convenient genetic tools, in particular the limited effectiveness of available reporter systems, has notably limited the toolbox for fundamental and applied studi...

journal_title：Applied and environmental microbiology

authors： Vannini A,Agriesti F,Mosca F,Roncarati D,Scarlato V,Danielli A

更新日期：2012-09-01 00:00:00

abstract：:Cold shock at 0 to 15 degrees C for 1 to 3 h increased the thermal sensitivity of Listeria monocytogenes. In a model broth system, thermal death time at 60 degrees C was reduced by up to 45% after L. monocytogenes Scott A was cold shocked for 3 h. The duration of the cold shock affected thermal tolerance more than did...

journal_title：Applied and environmental microbiology

authors： Miller AJ,Bayles DO,Eblen BS

更新日期：2000-10-01 00:00:00

abstract：:Five Pseudomonas aeruginosa strains were tested for the utilization of 47 low-molecular-weight compounds as their sole sources of carbon and energy for growth at a concentration of 2.5 g/liter. Of these compounds, 31 to 35 were consumed. Growth experiments in tap water at 15 degrees C were carried out with one particu...

journal_title：Applied and environmental microbiology

authors： van der Kooij D,Oranje JP,Hijnen WA

更新日期：1982-11-01 00:00:00

abstract：:Erwinia carotovora subsp. atroseptica, E. carotovora subsp. betavasculorum, and E. carotovora subsp. carotovora produce high levels of extracellular enzymes, such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt), and the quorum-sensing signal N-acyl-homoserine lactone (AHL) at 28 de...

journal_title：Applied and environmental microbiology

authors： Hasegawa H,Chatterjee A,Cui Y,Chatterjee AK

更新日期：2005-08-01 00:00:00

abstract：:Significant knowledge gaps exist in our understanding of Campylobacter jejuni contamination of the poultry production continuum. Microbiological surveillance and genotypic characterization were undertaken on C. jejuni isolates longitudinally recovered from three poultry farms (weekly samples), the abattoir at which bi...

journal_title：Applied and environmental microbiology

authors： Inglis GD,Ramezani N,Taboada EN,Boras VF,Uwiera RRE

更新日期：2021-01-15 00:00:00

abstract：:Fermentative production of succinic acid from glucose by Escherichia coli was significantly increased by overexpression of phosphoenolpyruvate carboxylase. In contrast, overexpression of phosphoenolpyruvate carboxykinase had no effect. Under optimized conditions, induction of the carboxylase resulted in a 3.5-fold inc...

journal_title：Applied and environmental microbiology

authors： Millard CS,Chao YP,Liao JC,Donnelly MI

更新日期：1996-05-01 00:00:00

abstract：:Miniaturized growth systems for heterogeneous culture collections are not only attractive in reducing demands for incubation space and medium but also in making the parallel handling of large numbers of strains more practicable. We report here on the optimization of oxygen transfer rates in deep-well microtiter plates...

journal_title：Applied and environmental microbiology

authors： Duetz WA,Rüedi L,Hermann R,O'Connor K,Büchs J,Witholt B

更新日期：2000-06-01 00:00:00

abstract：:In raw sludges and in mesophilically and thermophilically digested anaerobic sludges, large variations in numbers of viruses occurred over narrow ranges of numbers of fecal coliforms, total coliforms, and fecal streptococci, demonstrating that the bacteria were poor quantitative reflectors of the numbers of the viruse...

journal_title：Applied and environmental microbiology

authors： Berg G,Berman D

更新日期：1980-02-01 00:00:00

abstract：:Thiopeptide antibiotics are an important class of natural products resulting from posttranslational modifications of ribosomally synthesized peptides. Cyclothiazomycin is a typical thiopeptide antibiotic that has a unique bridged macrocyclic structure derived from an 18-amino-acid structural peptide. Here we reported ...

journal_title：Applied and environmental microbiology

authors： Wang J,Yu Y,Tang K,Liu W,He X,Huang X,Deng Z

更新日期：2010-04-01 00:00:00

abstract：:A novel technique, combining labelling and stereological methods, for the determination of spatial distribution of two microorganisms in a biofilm is presented. Cells of Nitrosomonas europaea (ATCC 19718) and Nitrobacter agilis (ATCC 14123) were homogeneously distributed in a kappa-carrageenan gel during immobilizatio...

journal_title：Applied and environmental microbiology

authors： Hunik JH,van den Hoogen MP,de Boer W,Smit M,Tramper J

更新日期：1993-06-01 00:00:00

abstract：:DNA was extracted from dry standing dead Spartina alterniflora stalks as well as dry Spartina wrack from the North Inlet (South Carolina) and Sapelo Island (Georgia) salt marshes. Partial nifH sequences were PCR amplified, the products were separated by denaturing gradient gel electrophoresis (DGGE), and the prominent...

journal_title：Applied and environmental microbiology

authors： Lovell CR,Friez MJ,Longshore JW,Bagwell CE

更新日期：2001-11-01 00:00:00

abstract：:Two genetically engineered microorganisms (GEMs), Pseudomonas sp. strain B13 FR1(pFRC20P) (FR120) and Pseudomonas putida KT2440(pWWO-EB62) (EB62), were introduced into activated sludge microcosms that had the level of aeration, nutrient makeup, and microbial community structure of activated sludge reactors. FR120 cont...

journal_title：Applied and environmental microbiology

authors： Nüsslein K,Maris D,Timmis K,Dwyer DF

更新日期：1992-10-01 00:00:00

abstract：:Brevibacterium linens M18, isolated from red smear cheese, produces a substance that inhibits the growth of Listeria spp. and several coryneform and other gram-positive bacteria. No gram-negative bacteria were inhibited. The substance is heat labile, sensitive to proteolytic enzymes, and stable between pH 3 and 12. Hi...

journal_title：Applied and environmental microbiology

authors： Valdés-Stauber N,Scherer S

更新日期：1994-10-01 00:00:00

abstract：:Bacteria in the genus Streptomyces and its close relatives are prolific producers of secondary metabolites with antibiotic activity. Genome sequencing of these bacteria has revealed a rich source of potentially new antibiotic pathways, whose products have never been observed. Moreover, these new pathways can provide n...

journal_title：Applied and environmental microbiology

authors： Fayed B,Ashford DA,Hashem AM,Amin MA,El Gazayerly ON,Gregory MA,Smith MC

更新日期：2015-12-01 00:00:00

abstract：:Five rumen bacteria, Selenomonas ruminantium, Bacteroides ruminicola, Megasphaera elsdenii, Butyrivibrio fibrisolvens, and Streptococcus bovis were grown in media containing nonlimiting concentrations of glucose, sucrose, maltose, cellobiose, xylose and/or lactate. Each bacterium was grown with every substrate that it...

journal_title：Applied and environmental microbiology

authors： Russell JB,Delfino FJ,Baldwin RL

更新日期：1979-03-01 00:00:00

abstract：:Characterization of the mle locus harboring the malolactic enzyme gene mleA and malate permease gene mleP from Leuconostoc oenos was completed in this study by mRNA analysis. Northern (RNA) blot experiments revealed a 2.6-kb transcript, suggesting an operon structure harboring mleA and mleP genes. Primer extension ana...

journal_title：Applied and environmental microbiology

authors： Labarre C,Diviès C,Guzzo J

更新日期：1996-12-01 00:00:00

abstract：:It has been hypothesized that the environment can influence the composition of the nasal microbiota. However, the direct influence of pig farming on the anterior and posterior nasal microbiota is unknown. Using a cross-sectional design, pig farms (n = 28) were visited in 2014 to 2015, and nasal swabs from 43 pig farme...

journal_title：Applied and environmental microbiology

authors： Kraemer JG,Ramette A,Aebi S,Oppliger A,Hilty M

更新日期：2018-03-01 00:00:00

abstract：:Nontoxigenic strains of Clostridium botulinum types C and D are converted to toxigenic strains by infection with specific Tox+ bacteriophages. The nucleic acids were extracted from five converting phages, c-st, c-468, c-203, c-d6f, and d-1873, and one nonconverting phage, c-n71, and treated with nucleases. The nucleic...

journal_title：Applied and environmental microbiology

authors： Fujii N,Oguma K,Yokosawa N,Kimura K,Tsuzuki K

更新日期：1988-01-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen and has been established as a model organism to study bacterial biofilm formation. At least three exopolysaccharides (alginate, Psl, and Pel) contribute to the formation of biofilms in this organism. Here mutants deficient in the production of one or more of th...

journal_title：Applied and environmental microbiology

authors： Ghafoor A,Hay ID,Rehm BH

更新日期：2011-08-01 00:00:00

abstract：:Fully grown broth cultures of various fast- and slow-growing rhizobia were deliberately diluted with various diluents before their aseptic incorporation into autoclaved peat in polypropylene bags (aseptic method) or mixed with the peat autoclaved in trays (tray method). In a factorial experiment with the aseptic metho...

journal_title：Applied and environmental microbiology

authors： Somasegaran P

更新日期：1985-08-01 00:00:00