Abstract:
:Thirty years of intensive research have significantly contributed to our understanding of Helicobacter pylori biology and pathogenesis. However, the lack of convenient genetic tools, in particular the limited effectiveness of available reporter systems, has notably limited the toolbox for fundamental and applied studies. Here, we report the construction of a bioluminescent H. pylori reporter system based on the Photorhabdus luminescens luxCDABE cassette. The system is constituted of a promoterless lux acceptor strain in which promoters and sequences of interest can be conveniently introduced by double homologous recombination of a suicide transformation vector. We validate the robustness of this new lux reporter system in noninvasive in vivo monitoring of dynamic transcriptional responses of inducible as well as repressible promoters and demonstrate its suitability for the implementation of genetic screens in H. pylori.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Vannini A,Agriesti F,Mosca F,Roncarati D,Scarlato V,Danielli Adoi
10.1128/AEM.01252-12subject
Has Abstractpub_date
2012-09-01 00:00:00pages
6524-33issue
18eissn
0099-2240issn
1098-5336pii
AEM.01252-12journal_volume
78pub_type
杂志文章abstract::Low and high levels of resistance to the benzimidazole fungicides benomyl and thiophanate-methyl were observed in field isolates of Monilinia fructicola, which is the causative agent of brown rot of stone fruit. Isolates that had low levels of resistance (hereafter referred to as LR isolates) and high levels of resist...
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pub_type: 杂志文章
doi:10.1128/aem.69.12.7145-7152.2003
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doi:10.1128/AEM.60.10.3536-3542.1994
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.41.6.1331-1336.1981
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更新日期:1984-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.2.547-551.1993
更新日期:1993-02-01 00:00:00
abstract::[This corrects the article on p. 4430 in vol. 63.]. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1981-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2002-12-01 00:00:00