Thermal destruction of Clostridium botulinum spores suspended in tomato juice in aluminum thermal death time tubes.

abstract：:Seventy-two Listeria monocytogenes isolates originating from 10 different fish products of 12 producers and 47 isolates from human listeriosis cases were typed by serotyping and multilocus enzyme electrophoresis. Seventy-five of these isolates were further subtyped by restriction analysis of genomic DNA with the enzym...

journal_title：Applied and environmental microbiology

authors： Boerlin P,Boerlin-Petzold F,Bannerman E,Bille J,Jemmi T

更新日期：1997-04-01 00:00:00

abstract：UNLABELLED:Our goal was to develop a robust tagging method that can be used to track bacterial strains in vivo To address this challenge, we adapted two existing systems: a modular plasmid-based reporter system (pCS26) that has been used for high-throughput gene expression studies in Salmonella and Escherichia coli and...

journal_title：Applied and environmental microbiology

authors： Shivak DJ,MacKenzie KD,Watson NL,Pasternak JA,Jones BD,Wang Y,DeVinney R,Wilson HL,Surette MG,White AP

更新日期：2016-07-29 00:00:00

abstract：:Amino acid modifications of the Thermobifida fusca Cel9A-68 catalytic domain or carbohydrate binding module 3c (CBM3c) were combined to create enzymes with changed amino acids in both domains. Bacterial crystalline cellulose (BC) and swollen cellulose (SWC) assays of the expressed and purified enzymes showed that thre...

journal_title：Applied and environmental microbiology

authors： Li Y,Irwin DC,Wilson DB

更新日期：2010-04-01 00:00:00

abstract：:Indirect exposure to waterborne viruses increases the risk of infection, especially among children with frequent hand-to-mouth contacts. Here, we quantified the transfer of one bacteriophage (MS2) and two enteric viruses (adenovirus and coxsackievirus) from liquid to skin. MS2, a commonly used enteric virus surrogate,...

journal_title：Applied and environmental microbiology

authors： Pitol AK,Bischel HN,Boehm AB,Kohn T,Julian TR

更新日期：2018-10-30 00:00:00

abstract：:Mean pH values in pooled samples of foregut, midgut, and hindgut from adult Melanoplus sanguinipes, which had been raised in the laboratory on barley shoots and wheat bran, were 5.15, 6.39, and 5.98, respectively. Homogenates of midgut/hindgut sections and frass (feces) yielded colony counts of bacteria by the spread ...

journal_title：Applied and environmental microbiology

authors： Mead LJ,Khachatourians GG,Jones GA

更新日期：1988-05-01 00:00:00

abstract：:The degradation of the herbicides EPTC (S-ethyl dipropylthiocarbamate) and atrazine (2-chloro-4-ethyl-amino-6-isopropylamino-1,3,5-triazine) is associated with an indigenous plasmid in Rhodococcus sp. strain TE1. Plasmid DNA libraries of Rhodococcus sp. strain TE1 were constructed in a Rhodococcus-Escherichia coli shu...

journal_title：Applied and environmental microbiology

authors： Shao ZQ,Behki R

更新日期：1995-05-01 00:00:00

abstract：:Influenza virus has been found to persist in the environment for hours to days, allowing for secondary transmission of influenza via inanimate objects known as fomites. We evaluated the efficacy of heat and moisture for the decontamination of surfaces for the purpose of preventing of the spread of influenza. Aqueous s...

journal_title：Applied and environmental microbiology

authors： McDevitt J,Rudnick S,First M,Spengler J

更新日期：2010-06-01 00:00:00

abstract：:Acropora and Porites corals are important reef builders in the Indo-Pacific and Caribbean. Bacteria associated with mucus produced by Porites spp. and Acropora spp. from Caribbean (Punta Maroma, Mexico) and Indo-Pacific (Hoga and Sampela, Indonesia) reefs were determined. Analysis of pyrosequencing libraries showed th...

journal_title：Applied and environmental microbiology

authors： McKew BA,Dumbrell AJ,Daud SD,Hepburn L,Thorpe E,Mogensen L,Whitby C

更新日期：2012-08-01 00:00:00

abstract：:Escherichia coli O157:H7 has caused serious outbreaks of food-borne illness via transmission in a variety of food vehicles, including unpasteurized apple juice, dried salami, and spinach. To understand how this pathogen responds to the multiple stresses of the food environment, we compared global transcription pattern...

journal_title：Applied and environmental microbiology

authors： Bergholz TM,Vanaja SK,Whittam TS

更新日期：2009-06-01 00:00:00

abstract：:Copper appears to be influencing the distribution and abundance of phytoplankton in marine environments, and cyanobacteria are thought to be the most sensitive of the phytoplankton groups to copper toxicity. By using growth assays of phylogenetically divergent clades, we found that coastal strains of marine Synechococ...

journal_title：Applied and environmental microbiology

authors： Stuart RK,Dupont CL,Johnson DA,Paulsen IT,Palenik B

更新日期：2009-08-01 00:00:00

abstract：:We assayed the tolerance to solvents of three toluene-degrading Pseudomonas putida strains and Pseudomonas mendocina KR1 in liquid and soil systems. P. putida DOT-T1 tolerated concentrations of heptane, propylbenzene, octanol, and toluene of at least 10% (vol/vol), while P. putida F1 and EEZ15 grew well in the presenc...

journal_title：Applied and environmental microbiology

authors： Huertas MJ,Duque E,Marqués S,Ramos JL

更新日期：1998-01-01 00:00:00

abstract：:A total of 896 samples of retail fresh meats and poultry was assayed for Escherichia coli serogroup O157:H7 by a hydrophobic grid membrane filter-immunoblot procedure developed specifically to isolate the organism from foods. The procedure involves several steps, including selective enrichment, filtration of enrichmen...

journal_title：Applied and environmental microbiology

authors： Doyle MP,Schoeni JL

更新日期：1987-10-01 00:00:00

abstract：:Quorum sensing (QS) is mediated by small molecules and involved in diverse cellular functions, such as virulence, biofilm formation, secondary metabolism, and cell differentiation. In this study, we developed a rapid and effective screening tool based on a cell-free Escherichia coli-based expression system to identify...

journal_title：Applied and environmental microbiology

authors： Yang YH,Kim TW,Park SH,Lee K,Park HY,Song E,Joo HS,Kim YG,Hahn JS,Kim BG

更新日期：2009-10-01 00:00:00

abstract：:While some species in the Bacillus cereus group are well-characterized human pathogens (e.g., B. anthracis and B. cereus sensu stricto), the pathogenicity of other species (e.g., B. pseudomycoides) either has not been characterized or is presently not well understood. To provide an updated characterization of the path...

journal_title：Applied and environmental microbiology

authors： Miller RA,Jian J,Beno SM,Wiedmann M,Kovac J

更新日期：2018-03-01 00:00:00

abstract：:The genus of Marinobacter is one of the most ubiquitous in the global oceans and assumed to significantly impact various biogeochemical cycles. The genome structure and content of Marinobacter aquaeolei VT8 was analyzed and compared with those from other organisms with diverse adaptive strategies. Here, we report the ...

journal_title：Applied and environmental microbiology

authors： Singer E,Webb EA,Nelson WC,Heidelberg JF,Ivanova N,Pati A,Edwards KJ

更新日期：2011-04-01 00:00:00

abstract：:Emulsan is a polyanionic heteropolysaccharide bioemulsifier produced by Acinetobacter calcoaceticus RAG-1. A mixed bacterial population was obtained by enrichment culture that was capable of degrading emulsan and using it as a carbon source. From this mixed culture, an emulsan-degrading bacterium, termed YUV-1, was is...

journal_title：Applied and environmental microbiology

authors： Shoham Y,Rosenberg M,Rosenberg E

更新日期：1983-09-01 00:00:00

abstract：:Two rapid methods were evaluated for their extraction of plasmids from Clostridium perfringens. The first method involved lysis of 1 to 2 ml of C. perfringens culture by treatment with hyaluronidase, lysozyme, and sarcosyl. DNA, extracted with phenol-chloroform, was treated with RNase, boiled, and electrophoresed in a...

journal_title：Applied and environmental microbiology

authors： Mahony DE,Clark GA,Stringer MF,MacDonald MC,Duchesne DR,Mader JA

更新日期：1986-03-01 00:00:00

abstract：:Nitrobacter agilis was entrapped in calcium alginate beads and used as a floating bed supplied with a continuous flow of nitrite medium. Complete nitrite oxidation was achieved within 30 h, and the system could be maintained for at least 210 h. The immobilized Nitrobacter system was subjected to sulfur oxyanions, acid...

journal_title：Applied and environmental microbiology

authors： Tsai YL,Schlasner SM,Tuovinen OH

更新日期：1986-12-01 00:00:00

abstract：:Listeria monocytogenes can cause the serious infection listeriosis, which despite antibiotic treatment has a high mortality. Understanding the response of L. monocytogenes to antibiotic exposure is therefore important to ensure treatment success. Some bacteria survive antibiotic treatment by formation of persisters, w...

journal_title：Applied and environmental microbiology

authors： Knudsen GM,Ng Y,Gram L

更新日期：2013-12-01 00:00:00

abstract：:Transfer of plasmid-borne antibiotic resistance genes in Escherichia coli wild-type strains is possible by transformation under naturally occurring conditions in oligotrophic, aquatic environments containing physiologic concentrations of calcium. In contrast, transformation is suppressed in nitrogen-rich body fluids l...

journal_title：Applied and environmental microbiology

authors： Woegerbauer M,Jenni B,Thalhammer F,Graninger W,Burgmann H

更新日期：2002-01-01 00:00:00

abstract：:Four membrane filter methods for the enumeration of fecal coliforms were compared for accuracy, specificity, and recovery. Water samples were taken several times from 13 marine, 1 estuarine, and 4 freshwater sites around Puerto Rico, from pristine waters and waters receiving treated and untreated sewage and effluent f...

journal_title：Applied and environmental microbiology

authors： Santiago-Mercado J,Hazen TC

更新日期：1987-12-01 00:00:00

abstract：:Bacterial growth in biofilms is the major cause of recalcitrant biofouling in industrial processes and of persistent infections in clinical settings. The use of bacteriophage treatment to lyse bacteria in biofilms has attracted growing interest. In particular, many natural or engineered phages produce depolymerases to...

journal_title：Applied and environmental microbiology

authors： Pei R,Lamas-Samanamud GR

更新日期：2014-09-01 00:00:00

abstract：UNLABELLED:Avian and possum fecal droppings may negatively impact roof-harvested rainwater (RHRW) water quality due to the presence of zoonotic pathogens. This study was aimed at evaluating the performance characteristics of a possum feces-associated (PSM) marker by screening 210 fecal and wastewater samples from possu...

journal_title：Applied and environmental microbiology

authors： Ahmed W,Hamilton KA,Gyawali P,Toze S,Haas CN

更新日期：2016-06-30 00:00:00

abstract：:Both Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis produce mosquitocidal toxins during sporulation and are extensively used in the field for control of mosquito populations. All the known toxins of the latter organism are known to be encoded on a large plasmid, pBtoxis. In an attempt to combine the...

journal_title：Applied and environmental microbiology

authors： Gammon K,Jones GW,Hope SJ,de Oliveira CM,Regis L,Silva Filha MH,Dancer BN,Berry C

更新日期：2006-03-01 00:00:00

abstract：:Lactic and acetic acids were determined to be slightly synergistic in their inhibitory interrelationship against Salmonella typhimurium with the use of a modified toxicological model. ...

journal_title：Applied and environmental microbiology

authors： Rubin HE

更新日期：1978-10-01 00:00:00

abstract：:Free fatty acids are important flavor compounds in cheese. Propionibacterium freudenreichii is the main agent of their release through lipolysis in Swiss cheese. Our aim was to identify the esterase(s) involved in lipolysis by P. freudenreichii. We targeted two previously identified esterases: one secreted esterase, P...

journal_title：Applied and environmental microbiology

authors： Abeijón Mukdsi MC,Falentin H,Maillard MB,Chuat V,Medina RB,Parayre S,Thierry A

更新日期：2014-01-01 00:00:00

abstract：:Lactobacillus gasseri LA39 and L. reuteri LA6 isolated from feces of the same human infant were found to produce similar cyclic bacteriocins (named gassericin A and reutericin 6, respectively) that cannot be distinguished by molecular weights or primary amino acid sequences. However, reutericin 6 has a narrower spectr...

journal_title：Applied and environmental microbiology

authors： Kawai Y,Ishii Y,Arakawa K,Uemura K,Saitoh B,Nishimura J,Kitazawa H,Yamazaki Y,Tateno Y,Itoh T,Saito T

更新日期：2004-05-01 00:00:00

abstract：:A Bacillus sp. capable of utilizing phosphite and hypophosphite under anaerobic conditions was isolated from Cape Canerval soil samples. The organism was isolated on a glucose-mineral salts medium with phosphate deleted. Anaerobic cultivation of this isolate resulted in decreases in the hypophosphite or phosphite conc...

journal_title：Applied and environmental microbiology

authors： Foster TL,Winans L Jr,Helms SJ

更新日期：1978-05-01 00:00:00

abstract：:More than 40 bacterial strains belonging to the cosmopolitan Polynucleobacter necessarius cluster (Betaproteobacteria) were isolated from a broad spectrum of freshwater habitats located in three climatic zones. Sequences affiliated with the freshwater P. necessarius cluster are among the most frequently detected in st...

journal_title：Applied and environmental microbiology

authors： Hahn MW

更新日期：2003-09-01 00:00:00

abstract：:Ethanol caused altered mobility of the lipophilic probe 1,6-diphenyl-1,3,5-hexatriene in plasma membrane preparations of Saccharomyces cerevisiae. Because lipids had been shown to protect yeast cells against ethanol toxicity, sterols, fatty acids, proteins, and combinations of these were tested; however, protection fr...

journal_title：Applied and environmental microbiology

authors： Walker-Caprioglio HM,Rodriguez RJ,Parks LW

更新日期：1985-09-01 00:00:00