Cell-free Escherichia coli-based system to screen for quorum-sensing molecules interacting with quorum receptor proteins of Streptomyces coelicolor.

abstract：:Rhizopogon roseolus Corda (synonym Rhizopogon rubescens Tul.), an economically important edible mushroom associated with the Pinaceae (mostly Pinus sp.), has a global distribution resulting from the introduction of exotic trees into the Southern Hemisphere for plantation forestry. However, the marketability of R. rose...

journal_title：Applied and environmental microbiology

authors： Visnovsky SB,Guerin-Laguette A,Wang Y,Pitman AR

更新日期：2010-01-01 00:00:00

abstract：:Two Aeromonas hydrophila bacteriophages, Aeh1 and Aeh2, were isolated from sewage. Both phages showed binal symmetry. The dimensions of A. hydrophila phages Aeh1 and Aeh2 differed from those of the other Aeromonas phages. Also, phage Aeh2 was the largest Aeromonas phage studied to date. Phage Aeh1 formed small, clear ...

journal_title：Applied and environmental microbiology

authors： Chow MS,Rouf MA

更新日期：1983-05-01 00:00:00

abstract：:Binding of three Bacillus thuringiensis insecticidal crystal proteins (ICPs) to the midgut epithelium of Ostrinia nubilalis larvae was characterized by performing binding experiments with both isolated brush border membrane vesicles and gut tissue sections. Our results demonstrate that two independent ICP receptors ar...

journal_title：Applied and environmental microbiology

authors： Denolf P,Jansens S,Peferoen M,Degheele D,Van Rie J

更新日期：1993-06-01 00:00:00

abstract：:Statistical models were used to predict the effects of tryptone, glucose, yeast extract (TGY) and Mn on biomass formation of the highly radioresistant bacterium Deinococcus radiodurans. Results suggested that glucose had marginal effect on biomass buildup, but Mn was a significant factor for biomass formation. Mn also...

journal_title：Applied and environmental microbiology

authors： Zhang YM,Wong TY,Chen LY,Lin CS,Liu JK

更新日期：2000-01-01 00:00:00

abstract：:Bifidobacterium longum strain JDM301, a widely used commercial strain in China, encodes at least two MazEF-like modules and one RelBE-like toxin-antitoxin (TA) system in its chromosome, designated MazE1F1Bif, MazE2F2Bif, and RelBEBif, respectively. Bacterial TA systems play an important role in several stress response...

journal_title：Applied and environmental microbiology

authors： Wei Y,Li Y,Yang F,Wu Q,Liu D,Li X,Hua H,Liu X,Wang Y,Zheng K,Tang R

更新日期：2017-04-17 00:00:00

abstract：:We developed a chemiluminometric assay of beta-galactosidase in coliform bacteria, using a phenylgalactose-substituted 1,2-dioxetane derivative as a substrate. Permeabilization of cells is required to ensure the efficient cellular uptake of this compound. By this method, one coliform seeded in 100 ml of sterile water ...

journal_title：Applied and environmental microbiology

authors： Van Poucke SO,Nelis HJ

更新日期：1995-12-01 00:00:00

abstract：:Municipal wastewater includes human waste that contains both commensal and pathogenic enteric microorganisms, and this collective community microbiome can be monitored for community diseases. In a previous study, we assessed the salmonellosis disease burden using municipal wastewater from Honolulu, Hawaii, which was m...

journal_title：Applied and environmental microbiology

authors： Diemert S,Yan T

更新日期：2019-05-02 00:00:00

abstract：:An acclimated shake flask CO(2) evolution test was used to study the biodegradability of 14 commercial phthalate esters that are commonly used as plasticizers. Both CO(2) evolution (ultimate biodegradation) and loss of parent phthalate esters (primary biodegradation) were measured. With only a few exceptions, primary ...

journal_title：Applied and environmental microbiology

authors： Sugatt RH,O'grady DP,Banerjee S,Howard PH,Gledhill WE

更新日期：1984-04-01 00:00:00

abstract：:In this study, we used chemostat cultures to analyze the quantitative effects of the specific growth rate and respiration on the metabolism in Lactococcus lactis CHCC2862 and on the downstream robustness of cells after freezing or freeze-drying. Under anaerobic conditions, metabolism remained homofermentative, althoug...

journal_title：Applied and environmental microbiology

authors： Johanson A,Goel A,Olsson L,Franzén CJ

更新日期：2020-03-02 00:00:00

abstract：:Studies of small noncoding RNAs (sRNAs) have been conducted predominantly using culturable organisms, and the acquisition of further information about sRNAs from global environments containing uncultured organisms now is very important. In this study, hot spring water (57°C, pH 8.1) was collected directly from the und...

journal_title：Applied and environmental microbiology

authors： Murakami S,Fujishima K,Tomita M,Kanai A

更新日期：2012-02-01 00:00:00

abstract：:The aim of this work was to develop a strategy to isolate a morphologically stable mutant of Streptomyces ambofaciens ATCC 15154 which produced high titers of spiramycin. The rationale was to grow a nitrosoguanidine-mutated population for many generations under nonselective conditions followed by two cycles of protopl...

journal_title：Applied and environmental microbiology

authors： Ford LM,Eaton TE,Godfrey OW

更新日期：1990-11-01 00:00:00

abstract：:Lyme disease, caused by the bacterium Borrelia burgdorferi sensu stricto, is an emerging zoonotic disease in Canada and is vectored by the blacklegged tick, Ixodes scapularis. Here we used Bayesian analyses of sequence types (STs), determined by multilocus sequence typing (MLST), to investigate the phylogeography of B...

journal_title：Applied and environmental microbiology

authors： Mechai S,Margos G,Feil EJ,Lindsay LR,Ogden NH

更新日期：2015-02-01 00:00:00

abstract：:Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that supp...

journal_title：Applied and environmental microbiology

authors： García-González V,Govantes F,Shaw LJ,Burns RG,Santero E

更新日期：2003-12-01 00:00:00

abstract：:Highly methylated, long-chain celluloses strongly inhibited cellulose degradation by several species of cellulolytic bacteria of ruminal origin. Specifically, the inhibitory effects of methylcellulose on the growth of Ruminococcus flavefaciens FD1 were concentration dependent, with complete inhibition at 0.1% (wt/vol)...

journal_title：Applied and environmental microbiology

authors： Rasmussen MA,Hespell RB,White BA,Bothast RJ

更新日期：1988-04-01 00:00:00

abstract：:Oysters collected in late winter, when they were free of Vibrio vulnificus, were exposed in the organism in the laboratory. The oysters effectively concentrated the bacteria from seawater, but when the inoculum was removed, the bacteria were rapidly cleared from the oyster tissues. These results suggest that V. vulnif...

journal_title：Applied and environmental microbiology

authors： Kelly MT,Dinuzzo A

更新日期：1985-12-01 00:00:00

abstract：:The gut microbiota plays important roles in its host. However, how each microbiota member contributes to the behavior of the whole population is not known. In this study, we therefore determined protein expression in the cecal microbiota in chickens of selected ages and in 7-day-old chickens inoculated with different ...

journal_title：Applied and environmental microbiology

authors： Polansky O,Sekelova Z,Faldynova M,Sebkova A,Sisak F,Rychlik I

更新日期：2015-12-28 00:00:00

abstract：:Four enteric viruses, poliovirus type 1, echovirus type 1, reovirus type 3, and simian adenovirus SV-11, were concentrated from seeded 1.3-liter volumes of raw, finished, and granular activated carbon-treated waters by adsorption to 47-mm-diameter (17 cm2), electropositive ( Virosorb 1MDS ) filters at pH 7.5 or electr...

journal_title：Applied and environmental microbiology

authors： Sobsey MD,Glass JS

更新日期：1984-05-01 00:00:00

abstract：:The Alvord Basin in southeast Oregon contains a variety of hydrothermal features which have never been microbiologically characterized. A sampling of Murky Pot (61 degrees C; pH 7.1) led to the isolation of a novel arsenic-metabolizing organism (YeAs) which produces an arsenic sulfide mineral known as beta-realgar, a ...

journal_title：Applied and environmental microbiology

authors： Ledbetter RN,Connon SA,Neal AL,Dohnalkova A,Magnuson TS

更新日期：2007-09-01 00:00:00

abstract：:Two 16S rRNA-targeted oligonucleotide probes were designed for the archaeal kingdoms Euryachaeota and Crenarchaeota. Probe specificities were evaluated by nonradioactive dot blot hybridization against selected reference organisms. The successful application of fluorescent-probe derivatives for whole-cell hybridization...

journal_title：Applied and environmental microbiology

authors： Burggraf S,Mayer T,Amann R,Schadhauser S,Woese CR,Stetter KO

更新日期：1994-09-01 00:00:00

abstract：:Five cases of primary amoebic meningoencephalitis associated with swimming in freshwater lakes have been recorded in Florida over the past 14 years. The present study demonstrated that pathogenic Naegleria, the causative agent, is relatively widespread. Twelve of 26 lakes sampled only once yielded the amoeba. Populati...

journal_title：Applied and environmental microbiology

authors： Wellings FM,Amuso PT,Chang SL,Lewis AL

更新日期：1977-12-01 00:00:00

abstract：:A structural gene which codes for an extracellular lipase (EC 3.1.1.3) in Aeromonas hydrophila H3, which was isolated from a female hospitalized patient, was cloned in Escherichia coli by using pBR322 as a vector. Lipase purified from both A. hydrophila culture supernatant and the periplasmic fluids of E. coli contain...

journal_title：Applied and environmental microbiology

authors： Anguita J,Rodríguez Aparicio LB,Naharro G

更新日期：1993-08-01 00:00:00

abstract：:Many fungal pathogens have short generation times, large population sizes, and mixed reproductive systems, providing high potential to adapt to heterogeneous environments of agroecosystems. Such adaptation complicates disease management and threatens food production. A better understanding of pathogen population biolo...

journal_title：Applied and environmental microbiology

authors： Kozhar O,Larsen MM,Grünwald NJ,Peever TL

更新日期：2020-04-17 00:00:00

abstract：:Alkyl glycosides are attractive surfactants because of their high surface activity and good biodegradability and can be produced from renewable resources. Through enzymatic catalysis, one can obtain well-defined alkyl glycosides, something that is very difficult to do using conventional chemistry. However, there is a ...

journal_title：Applied and environmental microbiology

authors： Lundemo P,Adlercreutz P,Karlsson EN

更新日期：2013-06-01 00:00:00

abstract：:The diversity of the methanotrophic community in mildly acidic landfill cover soil was assessed by three methods: two culture-independent molecular approaches and a traditional culture-based approach. For the first of the molecular studies, two primer pairs specific for the 16S rRNA gene of validly published type I (i...

journal_title：Applied and environmental microbiology

authors： Wise MG,McArthur JV,Shimkets LJ

更新日期：1999-11-01 00:00:00

abstract：:A gene that codes for a novel intracellular poly(3-hydroxybutyrate) (PHB) depolymerase, designated PhaZ1, has been identified in the genome of Bacillus megaterium. A native PHB (nPHB) granule-binding assay showed that purified soluble PhaZ1 had strong affinity for nPHB granules. Turbidimetric analyses revealed that Ph...

journal_title：Applied and environmental microbiology

authors： Chen HJ,Pan SC,Shaw GC

更新日期：2009-08-01 00:00:00

abstract：UNLABELLED:By combining genomics and isotope imaging analysis using high-resolution secondary ion mass spectrometry (NanoSIMS), we examined the function and evolution of Bacteroidales ectosymbionts of the protist Barbulanympha from the hindguts of the wood-eating cockroach Cryptocercus punctulatus In particular, we inv...

journal_title：Applied and environmental microbiology

authors： Tai V,Carpenter KJ,Weber PK,Nalepa CA,Perlman SJ,Keeling PJ

更新日期：2016-07-15 00:00:00

abstract：:The bacterial pathogen Vibrio vulnificus is found naturally in brackish coastal waters but can be greatly concentrated by filter-feeding organisms such as shellfish. Numerous experiments in which exogenous V. vulnificus cells are added to oysters in an attempt to measure uptake and depuration have been performed. In n...

journal_title：Applied and environmental microbiology

authors： Froelich B,Oliver J

更新日期：2013-09-01 00:00:00

abstract：:The milk supply is considered a primary route for a bioterrorism attack with Bacillus anthracis spores because typical high-temperature short-time (HTST) pasteurization conditions cannot inactivate spores. In the event of intentional contamination, an effective method to inactivate the spores in milk under HTST proces...

journal_title：Applied and environmental microbiology

authors： Xu S,Labuza TP,Diez-Gonzalez F

更新日期：2008-06-01 00:00:00

abstract：:A method was developed to follow bacterial nitrate reduction in freshwater sediments by using common high-performance liquid chromatographic equipment. The low detection limit (14 pmol) of the method enabled us to study concentration profiles and reaction kinetics under natural conditions. Significant nitrate concentr...

journal_title：Applied and environmental microbiology

authors： Hordijk CA,Snieder M,van Engelen JJ,Cappenberg TE

更新日期：1987-02-01 00:00:00

abstract：:The genes encoding NAD(+)-dependent alanine dehydrogenases (AlaDHs) (EC 1.4.1.1) from the Antarctic bacterial organisms Shewanella sp. strain Ac10 (SheAlaDH) and Carnobacterium sp. strain St2 (CarAlaDH) were cloned and expressed in Escherichia coli. Of all of the AlaDHs that have been sequenced, SheAlaDH exhibited the...

journal_title：Applied and environmental microbiology

authors： Galkin A,Kulakova L,Ashida H,Sawa Y,Esaki N

更新日期：1999-09-01 00:00:00