Identifying members of the domain Archaea with rRNA-targeted oligonucleotide probes.

Abstract:

:Two 16S rRNA-targeted oligonucleotide probes were designed for the archaeal kingdoms Euryachaeota and Crenarchaeota. Probe specificities were evaluated by nonradioactive dot blot hybridization against selected reference organisms. The successful application of fluorescent-probe derivatives for whole-cell hybridization required organism-specific optimizations of fixation and hybridization conditions to assure probe penetration and morphological integrity of the cells. The probes allowed preliminary grouping of three new hyperthermophilic isolates. Together with other group-specific rRNA-targeted oligonucleotide probes, these probes will facilitate rapid in situ monitoring of the populations present in hydrothermal systems and support cultivation attempts.

journal_name

Appl Environ Microbiol

authors

Burggraf S,Mayer T,Amann R,Schadhauser S,Woese CR,Stetter KO

doi

10.1128/AEM.60.9.3112-3119.1994

subject

Has Abstract

pub_date

1994-09-01 00:00:00

pages

3112-9

issue

9

eissn

0099-2240

issn

1098-5336

journal_volume

60

pub_type

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