Physiological characterization of viable-but-nonculturable Campylobacter jejuni cells.

abstract：:Kraft pulps, prepared from softwoods, and small chips of birch wood were treated with heme and tert-butyl hydroperoxide in aqueous solutions at reflux temperature. Analyses of treated pulps showed decreases in kappa number (a measure of lignin content) from about 36 to less than 2, with concomitant increases in bright...

journal_title：Applied and environmental microbiology

authors： Paszczyński A,Crawford RL,Blanchette RA

更新日期：1988-01-01 00:00:00

abstract：:Differences in individual host responses have emerged as an issue regarding the health benefits of probiotics. Here, we applied ribosome engineering (RE) technology, developed in an actinomycete study, to Lacticaseibacillus rhamnosus GG (LGG). RE can effectively enhance microbial potential by using antibiotics to indu...

journal_title：Applied and environmental microbiology

authors： Ishida M,Namai F,Shigemori S,Kajikawa S,Tsukagoshi M,Sato T,Ogita T,Shimosato T

更新日期：2020-10-01 00:00:00

abstract：:Microarray technology provides the opportunity to identify thousands of microbial genes or populations simultaneously, but low microbial biomass often prevents application of this technology to many natural microbial communities. We developed a whole-community genome amplification-assisted microarray detection approac...

journal_title：Applied and environmental microbiology

authors： Wu L,Liu X,Schadt CW,Zhou J

更新日期：2006-07-01 00:00:00

abstract：:We have developed a program that can accurately analyze the dynamic properties of tethered bacterial cells. The program works especially well with cells that tend to give rise to unstable rotations, such as polar-flagellated bacteria. The program has two novel components. The first dynamically adjusts the center of th...

journal_title：Applied and environmental microbiology

authors： Qian C,Wong CC,Swarup S,Chiam KH

更新日期：2013-08-01 00:00:00

abstract：:The goal of this field study was to provide insight into three distinct populations of microorganisms involved in in situ metabolism of phenol. Our approach measured 13CO2 respired from [13C]phenol and stable isotope probing (SIP) of soil DNA at an agricultural field site. Traditionally, SIP-based investigations have ...

journal_title：Applied and environmental microbiology

authors： DeRito CM,Pumphrey GM,Madsen EL

更新日期：2005-12-01 00:00:00

abstract：:Binding of three Bacillus thuringiensis insecticidal crystal proteins (ICPs) to the midgut epithelium of Ostrinia nubilalis larvae was characterized by performing binding experiments with both isolated brush border membrane vesicles and gut tissue sections. Our results demonstrate that two independent ICP receptors ar...

journal_title：Applied and environmental microbiology

authors： Denolf P,Jansens S,Peferoen M,Degheele D,Van Rie J

更新日期：1993-06-01 00:00:00

abstract：:Mineral soil-based inoculants of Rhizobium meliloti and Rhizobium phaseoli survived better at 4 degrees C than at higher temperatures, but ca. 15% of the cells were viable at 37 degrees C after 27 days. Soil-based inoculants of R. meliloti, R. phaseoli, Rhizobium japonicum, and a cowpea Rhizobium sp. applied to seeds ...

journal_title：Applied and environmental microbiology

authors： Chao WL,Alexander M

更新日期：1984-01-01 00:00:00

abstract：:With the submersible JAGO and by scuba diving we discovered three remarkable geothermal cones, rising 33, 25, and 45 m from the seafloor at a depth of 65 m in Eyjafjordur, northern Iceland. The greatest geothermal activity was on the highest cone, which discharged up to 50 liters of freshwater per s at 72 degrees C an...

journal_title：Applied and environmental microbiology

authors： Marteinsson VT,Kristjánsson JK,Kristmannsdóttir H,Dahlkvist M,Saemundsson K,Hannington M,Pétursdóttir SK,Geptner A,Stoffers P

更新日期：2001-02-01 00:00:00

abstract：:The cry1-type genes of Bacillus thuringiensis represent the largest cry gene family, which contains 50 distinct holotypes. It is becoming more and more difficult to identify cry1-type genes using current methods because of the increasing number of cry1-type genes. In the present study, an improved PCR-restriction frag...

journal_title：Applied and environmental microbiology

authors： Shu C,Liu D,Zhou Z,Cai J,Peng Q,Gao J,Song F,Zhang J

更新日期：2013-11-01 00:00:00

abstract：:The ability of indigenous Rhizobium leguminosarum and Rhizobium meliloti to use organic nutrients as growth substrates in soil was assessed by indirect bacteriophage analysis. A total of 17 organic compounds, including 9 carbohydrates, 3 organic acids, and 5 amino acids, were tested (1,000 mug g) in three soils with d...

journal_title：Applied and environmental microbiology

authors： Germida JJ

更新日期：1988-01-01 00:00:00

abstract：:An aminopeptidase was purified from cell extracts of Lactococcus lactis subsp. cremoris AM2 by ion-exchange chromatography. After electrophoresis of the purified enzyme in the presence or absence of sodium dodecyl sulfate, one protein band was detected. The enzyme was a 300-kilodalton hexamer composed of identical sub...

journal_title：Applied and environmental microbiology

authors： Neviani E,Boquien CY,Monnet V,Thanh LP,Gripon JC

更新日期：1989-09-01 00:00:00

abstract：:Genomic DNA was isolated from Cryptosporidium parvum oocysts by a specific immunomagnetic separation-in vitro excystation procedure and subjected to randomly amplified polymorphic DNA analysis using sequence-independent primers. An estuary C. parvum isolate was easily differentiated from several bovine isolates, while...

journal_title：Applied and environmental microbiology

authors： Deng MQ,Cliver DO

更新日期：1998-05-01 00:00:00

abstract：UNLABELLED:Rhizobia are best known for nodulating legume roots and fixing atmospheric nitrogen for the host in exchange for photosynthates. However, the majority of the diverse strains of rhizobia do not form nodules on legumes, often because they lack key loci that are needed to induce nodulation. Nonnodulating rhizob...

journal_title：Applied and environmental microbiology

authors： Gano-Cohen KA,Stokes PJ,Blanton MA,Wendlandt CE,Hollowell AC,Regus JU,Kim D,Patel S,Pahua VJ,Sachs JL

更新日期：2016-08-15 00:00:00

abstract：:The effect of selenite on the growth rate and protein synthesis has been investigated in Rhodobacter sphaeroides. This photosynthetic bacterium efficiently reduces selenite with intracellular accumulation under both dark aerobic and anaerobic photosynthetic conditions. Addition of 1 mM selenite under these two growth ...

journal_title：Applied and environmental microbiology

authors： Bebien M,Chauvin JP,Adriano JM,Grosse S,Verméglio A

更新日期：2001-10-01 00:00:00

abstract：:Lysostaphin represents a promising therapeutic agent for the treatment of staphylococcal infections, in particular those of methicillin-resistant Staphylococcus aureus (MRSA). However, conventional expression systems for the enzyme suffer from various limitations, and there remains a need for an efficient and cost-eff...

journal_title：Applied and environmental microbiology

authors： Zhao H,Blazanovic K,Choi Y,Bailey-Kellogg C,Griswold KE

更新日期：2014-05-01 00:00:00

abstract：:The beta-agarase C gene (agaC) of a marine bacterium, Vibrio sp. strain PO-303, consisted of 1,437 bp encoding 478 amino acid residues. beta-Agarase C was identified as the first beta-agarase that cannot hydrolyze neoagarooctaose and smaller neoagarooligosaccharides and was assigned to a novel glycoside hydrolase fami...

journal_title：Applied and environmental microbiology

authors： Dong J,Hashikawa S,Konishi T,Tamaru Y,Araki T

更新日期：2006-09-01 00:00:00

abstract：:During the fermentation of sake, cells of Saccharomyces cerevisiae are exposed to high concentrations of ethanol, thereby damaging the cell membrane and functional proteins. L-proline protects yeast cells from damage caused by freezing or oxidative stress. In this study, we evaluated the role of intracellular L-prolin...

journal_title：Applied and environmental microbiology

authors： Takagi H,Takaoka M,Kawaguchi A,Kubo Y

更新日期：2005-12-01 00:00:00

abstract：:Sputter coating of leaf litter microbe samples provides scanning electron microscope images with greater information content than either vacuum evaporation of thin metal coatings or tissue conductance. ...

journal_title：Applied and environmental microbiology

authors： Draggan S

更新日期：1976-02-01 00:00:00

abstract：:The high risk associated with biological threat agents dictates that any suspicious sample be handled under strict surety and safety controls and processed under high-level containment in specialized laboratories. This study attempted to find a rapid, reliable, and simple method for the complete inactivation of a wide...

journal_title：Applied and environmental microbiology

authors： Sagripanti JL,Hülseweh B,Grote G,Voss L,Böhling K,Marschall HJ

更新日期：2011-10-01 00:00:00

abstract：:An assembly consisting of Styrofoam cups with membranes of varying porosities was developed to study microorganism-root interactions. The assembly permitted uniform distribution of a bacterium in soil and was simple, easy to use, and disposable. In tests with the bacterium Pseudomonas solanacearum, little difference i...

journal_title：Applied and environmental microbiology

authors： Hartel PG,Billingsley JW,Williamson JW

更新日期：1989-05-01 00:00:00

abstract：:Most strains of Butyrivibrio fibrisolvens are known to produce extracellular polysaccharides (EPs). However, the rheological and functional properties of these EPs have not been determined. Initially, 26 strains of Butyrivibrio were screened for EP yield and apparent viscosities of cell-free supernatants. Yields range...

journal_title：Applied and environmental microbiology

authors： Ha YW,Stack RJ,Hespell RB,Gordon SH,Bothast RJ

更新日期：1991-07-01 00:00:00

abstract：:The broad-host-range (BHR) conjugative plasmids have developed diverse adaptive mechanisms defining the range of their promiscuity. The BHR conjugative RA3 plasmid, the archetype of the IncU group, can transfer between, replicate in, and be maintained in representatives of Alpha-, Beta-, and Gammaproteobacteria Its st...

journal_title：Applied and environmental microbiology

authors： Lewicka E,Dolowy P,Godziszewska J,Litwin E,Ludwiczak M,Jagura-Burdzy G

更新日期：2020-08-03 00:00:00

abstract：:Located in southwest China, the Aha watershed is continually contaminated by acid mine drainage (AMD) produced from upstream abandoned coal mines. The watershed is fed by creeks with elevated concentrations of aqueous Fe (total Fe > 1 g/liter) and SO4 (2-) (>6 g/liter). AMD contamination gradually decreases throughout...

journal_title：Applied and environmental microbiology

authors： Sun W,Xiao T,Sun M,Dong Y,Ning Z,Xiao E,Tang S,Li J

更新日期：2015-08-01 00:00:00

abstract：:Infectious hematopoietic necrosis virus (IHNV) is a pathogen of young salmon and trout. Viral epizootics among these fish in private and public rearing facilities have been a problem in the northwestern United States from California to Alaska, and an IHNV vaccine has been sought by the aquaculture experts. Since an IH...

journal_title：Applied and environmental microbiology

authors： Engelking HM,Harry JB,Leong JA

更新日期：1991-05-01 00:00:00

abstract：:The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (Sparus aurata) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 Pseudomonas strains isolat...

journal_title：Applied and environmental microbiology

authors： Tryfinopoulou P,Tsakalidou E,Nychas GJ

更新日期：2002-01-01 00:00:00

abstract：:A dense exopolysaccharide (EPS) matrix is crucial for cyanobacterial survival in terrestrial xeric environments, in which cyanobacteria undergo frequent expansion and shrinkage processes during environmental desiccation-rehydration cycles. However, it is unclear how terrestrial cyanobacteria coordinate the structural ...

journal_title：Applied and environmental microbiology

authors： Liu W,Cui L,Xu H,Zhu Z,Gao X

更新日期：2017-10-31 00:00:00

abstract：:Francisella tularensis has been associated with naturally occurring waterborne outbreaks and is also of interest as a potential biological weapon. Recovery of this pathogen from water using cultural methods is challenging due to the organism's fastidious growth requirements and interference by indigenous bacteria. A 1...

journal_title：Applied and environmental microbiology

authors： Humrighouse BW,Adcock NJ,Rice EW

更新日期：2011-09-01 00:00:00

abstract：:Microcosms were prepared from aquifer material, spiked with monoaromatic hydrocarbons, and amended with oxygen, nitrate, and nitrous oxide. Benzene and alkylbenzenes were degraded to concentrations below 5 micrograms/liter within 7 days under aerobic conditions, whereas only the alkylbenzenes were degraded when either...

journal_title：Applied and environmental microbiology

authors： Hutchins SR

更新日期：1991-08-01 00:00:00

abstract：:Pfiesteria piscicida is a heterotrophic dinoflagellate widely distributed along the middle Atlantic shore of the United States and associated with fish kills in the Neuse River (North Carolina) and the Chesapeake Bay (Maryland and Virginia). We constructed a genomic DNA library from clonally cultured P. piscicida and ...

journal_title：Applied and environmental microbiology

authors： Saito K,Drgon T,Robledo JA,Krupatkina DN,Vasta GR

更新日期：2002-11-01 00:00:00

abstract：:Studies of time-dependent drug and environmental effects on single, live bacterial cells would benefit significantly from a permeable, nonperturbative, long-lived fluorescent stain specific to the nucleoids (chromosomal DNA). The ideal stain would not affect cell growth rate or nucleoid morphology and dynamics, even d...

journal_title：Applied and environmental microbiology

authors： Bakshi S,Choi H,Rangarajan N,Barns KJ,Bratton BP,Weisshaar JC

更新日期：2014-08-01 00:00:00