Use of acid treatment and a selective medium to enhance the recovery of Francisella tularensis from water.

Abstract:

:Francisella tularensis has been associated with naturally occurring waterborne outbreaks and is also of interest as a potential biological weapon. Recovery of this pathogen from water using cultural methods is challenging due to the organism's fastidious growth requirements and interference by indigenous bacteria. A 15-min acid treatment procedure prior to culture on a selective agar was evaluated for recovery of F. tularensis from seeded water samples. Mean levels of reduction of virulent strains of F. tularensis subsp. holarctica and subsp. tularensis were less than 20% following acid treatment. The attenuated live vaccine strain (LVS) was less resistant to acid exposure. The acid treatment procedure coupled with plating on cystine heart agar with rabbit blood and antibiotics (CHARBab) allowed the isolation of F. tularensis seeded into five natural water samples.

journal_name

Appl Environ Microbiol

authors

Humrighouse BW,Adcock NJ,Rice EW

doi

10.1128/AEM.05226-11

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

6729-32

issue

18

eissn

0099-2240

issn

1098-5336

pii

AEM.05226-11

journal_volume

77

pub_type

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