Abstract:
:Francisella tularensis has been associated with naturally occurring waterborne outbreaks and is also of interest as a potential biological weapon. Recovery of this pathogen from water using cultural methods is challenging due to the organism's fastidious growth requirements and interference by indigenous bacteria. A 15-min acid treatment procedure prior to culture on a selective agar was evaluated for recovery of F. tularensis from seeded water samples. Mean levels of reduction of virulent strains of F. tularensis subsp. holarctica and subsp. tularensis were less than 20% following acid treatment. The attenuated live vaccine strain (LVS) was less resistant to acid exposure. The acid treatment procedure coupled with plating on cystine heart agar with rabbit blood and antibiotics (CHARBab) allowed the isolation of F. tularensis seeded into five natural water samples.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Humrighouse BW,Adcock NJ,Rice EWdoi
10.1128/AEM.05226-11subject
Has Abstractpub_date
2011-09-01 00:00:00pages
6729-32issue
18eissn
0099-2240issn
1098-5336pii
AEM.05226-11journal_volume
77pub_type
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