Heterologous Production of Cyanobacterial Mycosporine-Like Amino Acids Mycosporine-Ornithine and Mycosporine-Lysine in Escherichia coli.

abstract：:The ability of fractions of dissolved organic carbon (DOC) of different molecular weights (MW) to support bacterial growth was studied in batch culture experiments. Natural pelagic bacteria were inoculated into particle-free (0.2-mum filtered) water, taken from 10 oligotrophic lakes of differing humic content, and eit...

journal_title：Applied and environmental microbiology

authors： Tranvik LJ

更新日期：1990-06-01 00:00:00

abstract：:Previously, we demonstrated conjugal transfer of a specially constructed shuttle vector, pRDB5, from the human colonic anaerobe Bacteroides uniformis to the ruminal anaerobe Prevotella (Bacteroides) ruminicola B(1)4. We have now shown that naturally occurring gene transfer elements in Bacteroides species and Prevotell...

journal_title：Applied and environmental microbiology

authors： Shoemaker NB,Wang GR,Salyers AA

更新日期：1992-04-01 00:00:00

abstract：:Various combinations of the genes cryIVA (cry4A), cryIVD (cry11A), and p20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 by means of Escherichia coli-Anabaena shuttle vector pRL488p and were expressed under control of two tandem stro...

journal_title：Applied and environmental microbiology

authors： Xiaoqiang W,Vennison SJ,Huirong L,Ben-Dov E,Zaritsky A,Boussiba S

更新日期：1997-12-01 00:00:00

abstract：:This work describes the purification and characterization of propionicin F, the first bacteriocin isolated from Propionibacterium freudenreichii. The bacteriocin has a bactericidal activity and is only active against strains of P. freudenreichii. Propionicin F appears to be formed through a processing pathway new to b...

journal_title：Applied and environmental microbiology

authors： Brede DA,Faye T,Johnsborg O,Odegård I,Nes IF,Holo H

更新日期：2004-12-01 00:00:00

abstract：:A method to detect viable Cryptosporidium parvum oocysts was developed. Polyclonal immunoglobulin G against C. parvum oocyst and sporozoite surface antigens was purified from rabbit immune serum, biotinylated, and bound to streptoavidin-coated magnetic particles. C. parvum oocysts were captured by a specific antigen-a...

journal_title：Applied and environmental microbiology

authors： Deng MQ,Cliver DO,Mariam TW

更新日期：1997-08-01 00:00:00

abstract：:UNL-1, a lytic virus of Pseudomonas aeruginosa, was observed to express a novel inducible DNA damage reactivation activity in UV-A-irradiated P. aeruginosa host cells. The expression of bacteriophage reactivation was quantified in hosts exposed to either UV-C or UV-A radiation. While reactivation of UV-C-damaged UNL-1...

journal_title：Applied and environmental microbiology

authors： Shaffer JJ,Jacobsen LM,Schrader JO,Lee KW,Martin EL,Kokjohn TA

更新日期：1999-06-01 00:00:00

abstract：:The ever-increasing industrial demand for biocatalysis necessitates innovations in the preparation and stabilization of biocatalysts. In this study, we demonstrated that beta-galactosidase (beta-Gal) displayed on Bacillus spores by fusion to the spore coat proteins (CotG) may be used as a whole-cell immobilized biocat...

journal_title：Applied and environmental microbiology

authors： Kwon SJ,Jung HC,Pan JG

更新日期：2007-04-01 00:00:00

abstract：:Cr(VI) (chromate) is a widespread environmental contaminant. Bacterial chromate reductases can convert soluble and toxic chromate to the insoluble and less toxic Cr(III). Bioremediation can therefore be effective in removing chromate from the environment, especially if the bacterial propensity for such removal is enha...

journal_title：Applied and environmental microbiology

authors： Park CH,Keyhan M,Wielinga B,Fendorf S,Matin A

更新日期：2000-05-01 00:00:00

abstract：:The role of protease produced by a heterotrophic bacterium during growth was investigated with Aeromonas salmonicida, the pathogen of fish furunculosis, strain A-7301 and its protease-deficient mutant NTG-1 induced by mutagenesis. Strain A-7301 produced extracellular protease in a mixed amino acid medium (composed of ...

journal_title：Applied and environmental microbiology

authors： Sakai DK

更新日期：1985-10-01 00:00:00

abstract：:Specific Saccharomyces cerevisiae strains were recently found to be capable of efficiently utilizing inulin, but genetic mechanisms of inulin hydrolysis in yeast remain unknown. Here we report functional characteristics of invertase SUC2 from strain JZ1C and demonstrate that SUC2 is the key enzyme responsible for inul...

journal_title：Applied and environmental microbiology

authors： Wang SA,Li FL

更新日期：2013-01-01 00:00:00

abstract：:The physiology of ethanologenic Escherichia coli grown anaerobically in alkali-pretreated plant hydrolysates is complex and not well studied. To gain insight into how E. coli responds to such hydrolysates, we studied an E. coli K-12 ethanologen fermenting a hydrolysate prepared from corn stover pretreated by ammonia f...

journal_title：Applied and environmental microbiology

authors： Schwalbach MS,Keating DH,Tremaine M,Marner WD,Zhang Y,Bothfeld W,Higbee A,Grass JA,Cotten C,Reed JL,da Costa Sousa L,Jin M,Balan V,Ellinger J,Dale B,Kiley PJ,Landick R

更新日期：2012-05-01 00:00:00

abstract：UNLABELLED:Copper sulfate (CuSO4) has been widely used as an algicide to control harmful cyanobacterial blooms (CyanoHABs) in freshwater lakes. However, there are increasing concerns about this application, due mainly to the general toxicity of CuSO4 to other aquatic species and its long-term persistence in the environ...

journal_title：Applied and environmental microbiology

authors： Zhang BH,Ding ZG,Li HQ,Mou XZ,Zhang YQ,Yang JY,Zhou EM,Li WJ

更新日期：2016-08-15 00:00:00

abstract：:Studies have shown that certain opportunistic pathogenic species of nontuberculous mycobacteria (NTM) can be present in distributed drinking water. However, detailed information about NTM population composition in drinking water is lacking. Therefore, NTM communities in unchlorinated drinking water from the distributi...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Heijnen L,van der Kooij D

更新日期：2013-10-01 00:00:00

abstract：:The efficacy of currently available decontamination strategies for the treatment of indoor furnishings contaminated with bioterrorism agents is poorly understood. Efficacy testing of decontamination products in a controlled environment is needed to ensure that effective methods are used to decontaminate domestic and w...

journal_title：Applied and environmental microbiology

authors： Buttner MP,Cruz P,Stetzenbach LD,Klima-Comba AK,Stevens VL,Cronin TD

更新日期：2004-08-01 00:00:00

abstract：:The frequency of recovery of atypical mycobacteria was estimated in two treatment plants providing drinking water to Paris, France, at some intermediate stages of treatment. The two plants use two different filtration processes, rapid and slow sand filtration. Our results suggest that slow sand filtration is more effi...

journal_title：Applied and environmental microbiology

authors： Le Dantec C,Duguet JP,Montiel A,Dumoutier N,Dubrou S,Vincent V

更新日期：2002-11-01 00:00:00

abstract：:The occurrence and distribution of magnetotactic bacteria (MB) were studied as a function of the physical and chemical conditions in meromictic Salt Pond, Falmouth, Mass., throughout summer 2002. Three dominant MB morphotypes were observed to occur within the chemocline. Small microaerophilic magnetite-producing cocci...

journal_title：Applied and environmental microbiology

authors： Simmons SL,Sievert SM,Frankel RB,Bazylinski DA,Edwards KJ

更新日期：2004-10-01 00:00:00

abstract：:Lactococcus lactis subsp. cremoris MG1363 is a model for the lactic acid bacteria (LAB) used in the dairy industry. The proteolytic system, consisting of a proteinase, several peptide and amino acid uptake systems, and a host of intracellular peptidases, plays a vital role in nitrogen metabolism and is of eminent impo...

journal_title：Applied and environmental microbiology

authors： Huang C,Hernandez-Valdes JA,Kuipers OP,Kok J

更新日期：2020-04-01 00:00:00

abstract：:Cryptosporidium parvum is an apicomplexan parasite that infects humans and ruminants. C. parvum isolated from cattle in northeastern Turkey and in Israel was genotyped using multiple polymorphic genetic markers, and the two populations were compared to assess the effect of cattle husbandry on the parasite's population...

journal_title：Applied and environmental microbiology

authors： Tanriverdi S,Markovics A,Arslan MO,Itik A,Shkap V,Widmer G

更新日期：2006-04-01 00:00:00

abstract：:The polymerase chain reaction (PCR) and a radiolabeled oligonucleotide probe were used to specifically detect proteolytic and nonproteolytic Clostridium botulinum type B. Two synthetic primers deduced from the amino acid sequence data of type B neurotoxin were used to amplify a 1.5-kbp fragment corresponding to the li...

journal_title：Applied and environmental microbiology

authors： Szabo EA,Pemberton JM,Desmarchelier PM

更新日期：1992-01-01 00:00:00

abstract：:Previous attempts to express the toxin complex genes of Photorhabdus luminescens W14 in Escherichia coli have failed to reconstitute their oral toxicity to the model insect Manduca sexta. Here we show that the combination of three genes, tcdA, tcdB, and tccC, is essential for oral toxicity to M. sexta when expression ...

journal_title：Applied and environmental microbiology

authors： Waterfield N,Dowling A,Sharma S,Daborn PJ,Potter U,Ffrench-Constant RH

更新日期：2001-11-01 00:00:00

abstract：:White rot basidiomycetes were able to biodegrade styrene (1-phenylethene) graft copolymers of lignin containing different proportions of lignin and polystyrene [poly(1-phenylethylene)]. The biodegradation tests were run on lignin-styrene copolymerization products which contained 10.3, 32.2, and 50.4% (wt/wt) lignin. T...

journal_title：Applied and environmental microbiology

authors： Milstein O,Gersonde R,Huttermann A,Chen MJ,Meister JJ

更新日期：1992-10-01 00:00:00

abstract：:Deep-ocean hydrothermal-vent environments are rich in heavy metals and metalloids and present excellent sites for the isolation of metal-resistant microorganisms. Both metalloid-oxide-resistant and metalloid-oxide-reducing bacteria were found. Tellurite- and selenite-reducing strains were isolated in high numbers from...

journal_title：Applied and environmental microbiology

authors： Rathgeber C,Yurkova N,Stackebrandt E,Beatty JT,Yurkov V

更新日期：2002-09-01 00:00:00

abstract：:Conjugal plasmid transfer was examined on the phylloplane of bean (Phaseolus vulgaris) and related to the spatial distribution pattern and metabolic activity of the bacteria. The donor (Pseudomonas putida KT2442) harbored a derivative of the TOL plasmid, which conferred kanamycin resistance and had the gfp gene insert...

journal_title：Applied and environmental microbiology

authors： Normander B,Christensen BB,Molin S,Kroer N

更新日期：1998-05-01 00:00:00

abstract：:[This corrects the article on p. 469 in vol. 57.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1991-05-01 00:00:00

abstract：:Enterohemorrhagic Escherichia coli (EHEC) causes serious foodborne disease worldwide. It produces the very potent Shiga toxin 2 (Stx2). The Stx2-encoding genes are located on a prophage, and production of the toxin is linked to the synthesis of Stx phages. There is, currently, no good treatment for EHEC infections, as...

journal_title：Applied and environmental microbiology

authors： Kijewski A,Witsø IL,Iversen H,Rønning HT,L'Abée-Lund T,Wasteson Y,Lindbäck T,Aspholm M

更新日期：2020-11-24 00:00:00

abstract：:We developed a system to monitor the transfer of heterologous DNA from a genetically manipulated strain of Saccharomyces cerevisiae to Escherichia coli. This system is based on a yeast strain that carries multiple integrated copies of a pUC-derived plasmid. The bacterial sequences are maintained in the yeast genome by...

journal_title：Applied and environmental microbiology

authors： Adam AC,González-Blasco G,Rubio-Texeira M,Polaina J

更新日期：1999-12-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) released by infected individuals or present in human and hospital wastes can potentially cause contamination problems. The presence of HIV-1 was investigated in 16 environmental samples, including raw wastewater, sludge, final effluent, soil, and pond water, collected fr...

journal_title：Applied and environmental microbiology

authors： Ansari SA,Farrah SR,Chaudhry GR

更新日期：1992-12-01 00:00:00

abstract：:Total coliforms and total coliforms resistant to streptomycin, tetracycline, or chloramphenicol were isolated from filtered activated sludge effluents before and after UV light irradiation. Although the UV irradiation effectively disinfected the wastewater effluent, the percentage of the total surviving coliform popul...

journal_title：Applied and environmental microbiology

authors： Meckes MC

更新日期：1982-02-01 00:00:00

abstract：:Diverse nifH and nifH-like gene sequences were obtained from the deep-sea surface sediments of the methane hydrate-bearing Okhotsk Sea. Some sequences formed novel families of the NifH or NifH-like proteins, of currently unresolved bacterial or archaeal origin. Comparison with other marine environments indicates envir...

journal_title：Applied and environmental microbiology

authors： Dang H,Luan X,Zhao J,Li J

更新日期：2009-04-01 00:00:00

abstract：:Some species of ruminal bacteria are known to produce antimicrobial peptides, but the screening procedures have mostly been based on in vitro assays using standardized methods. Recent sequencing efforts have made available the genome sequences of hundreds of ruminal microorganisms. In this work, we performed genome mi...

journal_title：Applied and environmental microbiology

authors： Azevedo AC,Bento CB,Ruiz JC,Queiroz MV,Mantovani HC

更新日期：2015-10-01 00:00:00