Laboratory studies of disinfectants against Legionella pneumophila.

abstract：:A bioassay method was developed to measure the average intensity within a UV disinfection reactor. The survival of spores of Bacillus subtilis was determined as a function of UV dose to prepare a standard curve. Spores were added to unknown systems, and the survival rate was used to determine the average intensity. A ...

journal_title：Applied and environmental microbiology

authors： Qualls RG,Johnson JD

更新日期：1983-03-01 00:00:00

abstract：:Novel variants of Bacillus thuringiensis were isolated from the phylloplane of deciduous and conifer trees as well as of other plants. These isolates displayed a range of toxicity towards Trichoplusia ni. Immunoblot and toxin protein analysis indicate that these strains included representatives of the three principal ...

journal_title：Applied and environmental microbiology

authors： Smith RA,Couche GA

更新日期：1991-01-01 00:00:00

abstract：:A novel multiplex PCR method using three sets of specific primers was developed for the detection of the cytotoxic (act), heat-labile (alt), and heat-stable (ast) enterotoxin genes in Aeromonas spp. This assay was used to characterize 35 reference strains as well as 537 food-borne isolates. A total of seven gene patte...

journal_title：Applied and environmental microbiology

authors： Kingombe CI,D'Aoust JY,Huys G,Hofmann L,Rao M,Kwan J

更新日期：2010-01-01 00:00:00

abstract：:A highly virulent Serratia proteamaculans strain, AGR96X, exhibiting specific pathogenicity against larvae of the New Zealand grass grub (Costelytra giveni; Coleoptera: Scarabaeidae) and the New Zealand manuka beetle (Pyronota festiva and P. setosa; Coleoptera: Scarabaeidae), was isolated from a diseased grass grub la...

journal_title：Applied and environmental microbiology

authors： Hurst MRH,Beattie A,Jones SA,Laugraud A,van Koten C,Harper L

更新日期：2018-05-01 00:00:00

abstract：:Twenty-six lactic acid bacterium strains isolated from European dairy products were identified as Streptococcus thermophilus and characterized by bacterial growth and exopolysaccharide (EPS)-producing capacity in milk and enriched milk medium. In addition, the acidification rates of the different strains were compared...

journal_title：Applied and environmental microbiology

authors： Vaningelgem F,Zamfir M,Mozzi F,Adriany T,Vancanneyt M,Swings J,De Vuyst L

更新日期：2004-02-01 00:00:00

abstract：:Aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae CEN.PK113-7D were grown with different nitrogen sources. Cultures grown with phenylalanine, leucine, or methionine as a nitrogen source contained high levels of the corresponding fusel alcohols and organic acids, indicating activity of the Ehrlich...

journal_title：Applied and environmental microbiology

authors： Vuralhan Z,Luttik MA,Tai SL,Boer VM,Morais MA,Schipper D,Almering MJ,Kötter P,Dickinson JR,Daran JM,Pronk JT

更新日期：2005-06-01 00:00:00

abstract：:To investigate the effects of land use and crop management on soybean rhizobial communities, 280 nodule isolates were trapped from 7 fields with different land use and culture histories. Besides the known Bradyrhizobium japonicum, three novel genospecies were isolated from these fields. Grassland (GL) maintained a hig...

journal_title：Applied and environmental microbiology

authors： Yan J,Han XZ,Ji ZJ,Li Y,Wang ET,Xie ZH,Chen WF

更新日期：2014-09-01 00:00:00

abstract：:The response of Lactobacillus delbrueckii subsp. bulgaricus cells to heat stress was studied by use of a chemically defined medium. Two-dimensional electrophoresis (2-DE) analysis was used to correlate the kinetics of heat shock protein (HSP) induction with cell recovery from heat injury. We demonstrated that enhanced...

journal_title：Applied and environmental microbiology

authors： Gouesbet G,Jan G,Boyaval P

更新日期：2002-03-01 00:00:00

abstract：:A previously identified insert expressing an endopeptidase from a Lactobacillus helveticus CNRZ32 genomic library was characterized. Nucleotide sequence analysis revealed an open reading frame of 1,941 bp encoding a putative protein of 71.2 kDa which contained a zinc-protease motif. Protein homology searches revealed ...

journal_title：Applied and environmental microbiology

authors： Chen YS,Steele JL

更新日期：1998-09-01 00:00:00

abstract：:Unspecific peroxygenases (UPOs) constitute a new family of fungal heme-thiolate enzymes in which there is high biotechnological interest. Although several thousand genes encoding hypothetical UPO-type proteins have been identified in sequenced fungal genomes and other databases, only a few UPO enzymes have been experi...

journal_title：Applied and environmental microbiology

authors： Linde D,Olmedo A,González-Benjumea A,Estévez M,Renau-Mínguez C,Carro J,Fernández-Fueyo E,Gutiérrez A,Martínez AT

更新日期：2020-03-18 00:00:00

abstract：:Fusarium verticillioides (Gibberella fujikuroi mating population A [MP-A]) is a widespread pathogen on maize and is well-known for producing fumonisins, mycotoxins that cause severe disease in animals and humans. The species is a member of the Gibberella fujikuroi species complex, which consists of at least 11 differe...

journal_title：Applied and environmental microbiology

authors： Bömke C,Rojas MC,Hedden P,Tudzynski B

更新日期：2008-12-01 00:00:00

abstract：:Pseudomonas putida aggressively colonizes root surfaces and is agglutinated by a root surface glycoprotein. Mutants of P. putida derived chemically or by Tn5 insertion demonstrated enhanced or decreased agglutinability. Two nonagglutinable Tn5 mutants (Agg) and two mutants with enhanced agglutinability (Agg) possessed...

journal_title：Applied and environmental microbiology

authors： Anderson AJ,Habibzadegah-Tari P,Tepper CS

更新日期：1988-02-01 00:00:00

abstract：:Eight concentration and purification methods were evaluated to determine percentages of recovery of Cryptosporidium parvum oocysts from calf feces. The NaCl flotation method generally resulted in the highest percentages of recovery. Based on the percentages of recovery, the amounts of fecal debris in the final oocyst ...

journal_title：Applied and environmental microbiology

authors： Kuczynska E,Shelton DR

更新日期：1999-07-01 00:00:00

abstract：:Bacterial diversity in a deep-sea sediment was investigated by constructing actinobacterium-specific 16S ribosomal DNA (rDNA) clone libraries from sediment sections taken 5 to 12, 15 to 18, and 43 to 46 cm below the sea floor at a depth of 3,814 m. Clones were placed into operational taxonomic unit (OTU) groups with >...

journal_title：Applied and environmental microbiology

authors： Stach JE,Maldonado LA,Masson DG,Ward AC,Goodfellow M,Bull AT

更新日期：2003-10-01 00:00:00

abstract：:The effects of fixation on the cell volume of marine heterotrophic nanoflagellates and planktonic ciliates were investigated. Decreases in cell volume depended on the combination of the protozoan taxa and the particular fixative. For a particular fixative and protozoan species, degree of shrinkage was independent of p...

journal_title：Applied and environmental microbiology

authors： Choi JW,Stoecker DK

更新日期：1989-07-01 00:00:00

abstract：:In many cities, the feral rock dove is an abundant bird species that can harbor Chlamydophila psittaci. We determined the prevalence and genotype of C. psittaci in fresh fecal samples from feral pigeons in Amsterdam, The Netherlands. The prevalence was 7.9% overall (26/331; 95% confidence interval, 5 to 11). Ten genot...

journal_title：Applied and environmental microbiology

authors： Heddema ER,Ter Sluis S,Buys JA,Vandenbroucke-Grauls CM,van Wijnen JH,Visser CE

更新日期：2006-06-01 00:00:00

abstract：:A new method is described in which the Salkowski reaction is used for the rapid selection of alkaloid-producing mutants of the ergot fungus. This method was used to investigate the influence of a second mutation with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) on various mutants selected by a preliminary NTG mutation o...

journal_title：Applied and environmental microbiology

authors： Srikrai S,Robbers JE

更新日期：1983-04-01 00:00:00

abstract：:Low and high levels of resistance to the benzimidazole fungicides benomyl and thiophanate-methyl were observed in field isolates of Monilinia fructicola, which is the causative agent of brown rot of stone fruit. Isolates that had low levels of resistance (hereafter referred to as LR isolates) and high levels of resist...

journal_title：Applied and environmental microbiology

authors： Ma Z,Yoshimura MA,Michailides TJ

更新日期：2003-12-01 00:00:00

abstract：:A study was conducted to determine the influence of arbuscular mycorrhizal (AM) fungi on Salmonella and enterohemorrhagic Escherichia coli O157:H7 (EHEC) in autoclaved soil and translocation into leek plants. Six-week-old leek plants (with [Myc+] or without [Myc-] AM fungi) were inoculated with composite suspensions o...

journal_title：Applied and environmental microbiology

authors： Gurtler JB,Douds DD Jr,Dirks BP,Quinlan JJ,Nicholson AM,Phillips JG,Niemira BA

更新日期：2013-03-01 00:00:00

abstract：:Confocal scanning laser microscopy (CSLM) was used to demonstrate the attachment of Escherichia coli O157:H7 transformed with a plasmid encoding for green fluorescent protein (GFP) to the surface and within the internal structures of nonwaxed Red Delicious cv. apples. Apples at 2 or 25 degrees C were inoculated with a...

journal_title：Applied and environmental microbiology

authors： Burnett SL,Chen J,Beuchat LR

更新日期：2000-11-01 00:00:00

abstract：:Renibacterium salmoninarum, a gram-positive diplococcobacillus, causes bacterial kidney disease, a condition that can result in extensive morbidity and mortality among stocks of fish. An immunodominant extracellular protein, called major soluble antigen (MSA), is encoded by two identical genes, msa1 and msa2. We found...

journal_title：Applied and environmental microbiology

authors： Rhodes LD,Coady AM,Deinhard RK

更新日期：2004-11-01 00:00:00

abstract：:Recombinant plasmids containing fusion proteins composed of two different modules were constructed and expressed in Escherichia coli. The modules encoded the lactase LacA (LacZ) from the thermophilic bacterium Thermoanaerobacter ethanolicus and the cellulase CelD, a cellulose-binding module (CBM) from Anaerocellum the...

journal_title：Applied and environmental microbiology

authors： Velikodvorskaya GA,Tikhonova TV,Gurvits ID,Karyagina AS,Lavrova NV,Sergienko OV,Tashlitskii VN,Lunina NA,Lunin VG

更新日期：2010-12-01 00:00:00

abstract：:Xylella fastidiosa is a gram-negative, xylem-limited bacterium affecting economically important crops (e.g., grapevine, citrus, and coffee). The citrus variegated chlorosis (CVC) strain of X. fastidiosa is the causal agent of this severe disease of citrus in Brazil and represents the first plant-pathogenic bacterium f...

journal_title：Applied and environmental microbiology

authors： Monteiro PB,Teixeira DC,Palma RR,Garnier M,Bové JM,Renaudin J

更新日期：2001-05-01 00:00:00

abstract：:We present a tool for repetitive, marker-free, site-specific integration in Lactococcus lactis, in which a nonreplicating plasmid vector (pKV6) carrying a phage attachment site (attP) can be integrated into a bacterial attachment site (attB). The novelty of the tool described here is the inclusion of a minimal bacteri...

journal_title：Applied and environmental microbiology

authors： Petersen KV,Martinussen J,Jensen PR,Solem C

更新日期：2013-06-01 00:00:00

abstract：:Streptomyces exfoliatus SMF19 produced two types of extracellular beta-lactamase-inhibiting proteins, 48 (beta LIP-I) and 33 (beta LIP-II) kDa. The inhibition mode of beta LIP-I on Bacto Penase and benzylpenicillin as substrates was uncompetitive (i.e., both Km and Vmax were changed), while that of beta LIP-II was non...

journal_title：Applied and environmental microbiology

authors： Kim MK,Lee KJ

更新日期：1994-03-01 00:00:00

abstract：:Ammonia monooxygenase of Nitrosomonas europaea catalyzes the oxidation of alkanes (up to C(8)) to alcohols and alkenes (up to C(5)) to epoxides and alcohols in the presence of ammonium ions. Straight-chain, N-terminal alkynes (up to C(10)) all exhibited a time-dependent inhibition of ammonia oxidation without effects ...

journal_title：Applied and environmental microbiology

authors： Hyman MR,Murton IB,Arp DJ

更新日期：1988-12-01 00:00:00

abstract：:Alkyl glycosides are attractive surfactants because of their high surface activity and good biodegradability and can be produced from renewable resources. Through enzymatic catalysis, one can obtain well-defined alkyl glycosides, something that is very difficult to do using conventional chemistry. However, there is a ...

journal_title：Applied and environmental microbiology

authors： Lundemo P,Adlercreutz P,Karlsson EN

更新日期：2013-06-01 00:00:00

abstract：:beta-D-Galactosidase (EC 3.2.1.23) was extracted from Streptococcus thermophilus grown in deproteinized cheese whey. Cultural conditions optimum for maximum enzyme production were pH 7.0, 40 degrees C, and 24 h. Proteose peptone (2.0%, wt/vol) and corn steep liquor (2.8%, wt/vol) were highly stimulatory, increasing th...

journal_title：Applied and environmental microbiology

authors： Rao MV,Dutta SM

更新日期：1977-08-01 00:00:00

abstract：:A novel agar medium, chromogenic Salmonella esterase (CSE) agar, for the differentiation of salmonellae is described. The agar contains peptones and nutrient extracts together with the following (grams per liter unless otherwise specified): 4-[2-(4-octanoyloxy-3, 5-dimethoxyphenyl)-vinyl]-quinolinium-1-(propan-3-yl ca...

journal_title：Applied and environmental microbiology

authors： Cooke VM,Miles RJ,Price RG,Richardson AC

更新日期：1999-02-01 00:00:00

abstract：:Reverse transcription-PCR analysis of drinking water in the homes of 56 children suffering from rotaviral gastroenteritis has shown the presence of the rotavirus genome in four samples. These strains were different from human rotaviruses detected in the children's feces, as determined by sequencing of the VP7-amplifie...

journal_title：Applied and environmental microbiology

authors： Gratacap-Cavallier B,Genoulaz O,Brengel-Pesce K,Soule H,Innocenti-Francillard P,Bost M,Gofti L,Zmirou D,Seigneurin JM

更新日期：2000-06-01 00:00:00