Development and implementation of a multiplex single-nucleotide polymorphism genotyping assay for detection of virulence-attenuating mutations in the Listeria monocytogenes virulence-associated gene inlA.

abstract：:The dynamic control of gene expression is important for adjusting fluxes in order to obtain desired products and achieve appropriate cell growth, particularly when the synthesis of a desired product drains metabolites required for cell growth. For dynamic gene expression, a promoter responsive to a particular environm...

journal_title：Applied and environmental microbiology

authors： Yin X,Shin HD,Li J,Du G,Liu L,Chen J

更新日期：2017-03-02 00:00:00

abstract：UNLABELLED:Salmonella is recognized as one of the most significant enteric foodborne bacterial pathogens. In recent years, the resistance of pathogens to biocides and other environmental stresses, especially when they are embedded in biofilm structures, has led to the search for and development of novel antimicrobial s...

journal_title：Applied and environmental microbiology

authors： Karampoula F,Giaouris E,Deschamps J,Doulgeraki AI,Nychas GJ,Dubois-Brissonnet F

更新日期：2016-08-15 00:00:00

abstract：:The human large intestine is covered with a protective mucus coating, which is heavily colonized by complex bacterial populations that are distinct from those in the gut lumen. Little is known of the composition and metabolic activities of these biofilms, although they are likely to play an important role in mucus bre...

journal_title：Applied and environmental microbiology

authors： Macfarlane S,Woodmansey EJ,Macfarlane GT

更新日期：2005-11-01 00:00:00

abstract：:We found that the human intestinal isolate Bifidobacterium bifidum MIMBb75 strongly adhered to Caco-2 cells. Proteinase K and lithium chloride treatments showed that proteins play a key role in MIMBb75 adhesion to Caco-2 cells. By studying the cell wall-associated proteins, we identified a surface protein, which we la...

journal_title：Applied and environmental microbiology

authors： Guglielmetti S,Tamagnini I,Mora D,Minuzzo M,Scarafoni A,Arioli S,Hellman J,Karp M,Parini C

更新日期：2008-08-01 00:00:00

abstract：:The association of Vibrio cholerae with zooplankton has been suggested as an important factor in transmission of human epidemic cholera, and the ability to colonize zooplankton surfaces may play a role in the temporal variation and predominance of the two different serogroups (V. cholerae O1 El Tor and O139) in the aq...

journal_title：Applied and environmental microbiology

authors： Rawlings TK,Ruiz GM,Colwell RR

更新日期：2007-12-01 00:00:00

abstract：:A real-time PCR-based method targeting the 18S rRNA gene was developed for the quantitative detection of Hartmannella vermiformis, a free-living amoeba which is a potential host for Legionella pneumophila in warm water systems and cooling towers. The detection specificity was validated using genomic DNA of the closely...

journal_title：Applied and environmental microbiology

authors： Kuiper MW,Valster RM,Wullings BA,Boonstra H,Smidt H,van der Kooij D

更新日期：2006-09-01 00:00:00

abstract：:The ability of 135 Staphylococcus strains isolated from Spanish dry-cured hams to produce enterotoxins in culture was investigated by the reversed passive latex agglutination method. A high percentage of enterotoxigenic Staphylococcus aureus strains (85.9%) was recorded, and 54.3% of these produced enterotoxin A. One ...

journal_title：Applied and environmental microbiology

authors： Marín ME,de la Rosa MC,Cornejo I

更新日期：1992-03-01 00:00:00

abstract：:Streptococcus suis is an important pathogen of pigs and may cause serious disease in humans. Serotyping is an important tool for detection and epidemiological studies of S. suis Thirty-three reference serotypes and nine novel cps loci (NCLs) are recognized in S. suis To gain a better understanding of the prevalence an...

journal_title：Applied and environmental microbiology

authors： Qiu X,Bai X,Lan R,Zheng H,Xu J

更新日期：2016-11-21 00:00:00

abstract：:Colonization of livestock with bacteria resistant to antibiotics is considered a risk for the entry of drug-resistant pathogens into the food chain. For this reason, there is a need for novel concepts to address the eradication of drug-resistant commensals on farms. In the present report, we evaluated the decontaminat...

journal_title：Applied and environmental microbiology

authors： Schmithausen RM,Kellner SR,Schulze-Geisthoevel SV,Hack S,Engelhart S,Bodenstein I,Al-Sabti N,Reif M,Fimmers R,Körber-Irrgang B,Harlizius J,Hoerauf A,Exner M,Bierbaum G,Petersen B,Bekeredjian-Ding I

更新日期：2015-11-01 00:00:00

abstract：:The use of the food-grade bacterium Lactococcus lactis as a DNA delivery vehicle at the mucosal level is an attractive DNA vaccination strategy. Previous experiments showed that recombinant L. lactis expressing the Listeria monocytogenes inlA gene can deliver a functional gene into mammalian cells. Here, we explored t...

journal_title：Applied and environmental microbiology

authors： Guimarães VD,Innocentin S,Lefèvre F,Azevedo V,Wal JM,Langella P,Chatel JM

更新日期：2006-11-01 00:00:00

abstract：:A d-xylose reductase mutant of Pachysolen tannophilus was isolated on the basis of its poor growth on d-xylose but normal growth on xylitol and d-glucose. Fractionation of cell extracts indicated that the mutant was deficient in d-xylose reductase activity that used NADPH exclusively as a cofactor, but not in activity...

journal_title：Applied and environmental microbiology

authors： Schneider H,Lee H,Barbosa Mde F,Kubicek CP,James AP

更新日期：1989-11-01 00:00:00

abstract：:An inulinase-producing Microbulbifer sp. strain, JAM-3301, was isolated from a deep-sea sediment. An inulin operon that contained three open reading frames was cloned and sequenced. Two of the three genes were expressed. One product was an endo-inulinase, and the other was a β-fructofuranosidase. Both enzymes worked t...

journal_title：Applied and environmental microbiology

authors： Kobayashi T,Uchimura K,Deguchi S,Horikoshi K

更新日期：2012-04-01 00:00:00

abstract：:The genetic diversity of Borrelia burgdorferi sensu lato was assessed in a focus of Lyme borreliosis in southern Britain dominated by game birds. Ticks, rodents, and pheasants were analyzed for spirochete infections by PCR-targeting the 23S-5S rRNA genes, followed by genotyping by the reverse line blot method. In ques...

journal_title：Applied and environmental microbiology

authors： Kurtenbach K,Peacey M,Rijpkema SG,Hoodless AN,Nuttall PA,Randolph SE

更新日期：1998-04-01 00:00:00

abstract：:Cyanuric acid, a metabolic intermediate in the degradation of many s-triazine compounds, is further metabolized by cyanuric acid hydrolase. Cyanuric acid also accumulates in swimming pools due to the breakdown of the sanitizing agents di- and trichloroisocyanuric acid. Structurally stable cyanuric acid hydrolases are ...

journal_title：Applied and environmental microbiology

authors： Li Q,Seffernick JL,Sadowsky MJ,Wackett LP

更新日期：2009-11-01 00:00:00

abstract：:A total of 25 marine caulobacters were isolated from littoral marine sources. Several aspects of their physiology and morphology were examined, as well as their suitability for genetic manipulation in laboratory cultivation. Caulobacters were readily isolated from all sources, including samples from areas containing p...

journal_title：Applied and environmental microbiology

authors： Anast N,Smit J

更新日期：1988-03-01 00:00:00

abstract：:Francisella tularensis has been associated with naturally occurring waterborne outbreaks and is also of interest as a potential biological weapon. Recovery of this pathogen from water using cultural methods is challenging due to the organism's fastidious growth requirements and interference by indigenous bacteria. A 1...

journal_title：Applied and environmental microbiology

authors： Humrighouse BW,Adcock NJ,Rice EW

更新日期：2011-09-01 00:00:00

abstract：:A gene encoding a putative 150-amino-acid methylglyoxal synthase was identified in Clostridium acetobutylicum ATCC 824. The enzyme was overexpressed in Escherichia coli and purified. Methylglyoxal synthase has a native molecular mass of 60 kDa and an optimum pH of 7.5. The Km and Vmax values for the substrate dihydrox...

journal_title：Applied and environmental microbiology

authors： Huang K,Rudolph FB,Bennett GN

更新日期：1999-07-01 00:00:00

abstract：:Demineralization reduced heat resistance of B. subtilis spores, but the pattern and magnitude of the reduction depended on sporulation temperature and on heating menstruum pH. The differences in heat resistance of native spores caused by sporulation temperature almost disappeared after demineralization. Demineralized ...

journal_title：Applied and environmental microbiology

authors： Palop A,Sala FJ,Condón S

更新日期：1999-03-01 00:00:00

abstract：:Simkania negevensis is a recently discovered chlamydia-like intracellular microorganism which has been associated with bronchiolitis in infants and with community-acquired pneumonia in adults; a high seroprevalence of antibodies to the microorganism has been found in various population groups. S. negevensis can be gro...

journal_title：Applied and environmental microbiology

authors： Kahane S,Platzner N,Dvoskin B,Itzhaki A,Friedman MG

更新日期：2004-06-01 00:00:00

abstract：:Biphasic systems can overcome the problem of low productivity in conventional media and have been exploited for biocatalysis. Solvent-tolerant microorganisms are useful in biotransformation with whole cells in biphasic reactions. A solvent-tolerant desulfurizing bacterium, Pseudomonas putida A4, was constructed by int...

journal_title：Applied and environmental microbiology

authors： Tao F,Yu B,Xu P,Ma CQ

更新日期：2006-07-01 00:00:00

abstract：:Deep-ocean hydrothermal-vent environments are rich in heavy metals and metalloids and present excellent sites for the isolation of metal-resistant microorganisms. Both metalloid-oxide-resistant and metalloid-oxide-reducing bacteria were found. Tellurite- and selenite-reducing strains were isolated in high numbers from...

journal_title：Applied and environmental microbiology

authors： Rathgeber C,Yurkova N,Stackebrandt E,Beatty JT,Yurkov V

更新日期：2002-09-01 00:00:00

abstract：:Bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. This approach permitted separation of the lake isolates into two ...

journal_title：Applied and environmental microbiology

authors： Sjogren RE,Gibson MJ

更新日期：1981-06-01 00:00:00

abstract：:Two duck farms in Hong Kong were examined monthly for 1 year for the occurrence and persistence of influenza viruses within the duck communities. The predominant virus in one community was H3N2, a virus antigenically related to the pandemic Hong Kong strain. This virus was isolated monthly throughout the year from fec...

journal_title：Applied and environmental microbiology

authors： Markwell DD,Shortridge KF

更新日期：1982-01-01 00:00:00

abstract：:[This corrects the article on p. 2161 in vol. 59.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1993-12-01 00:00:00

abstract：:An acclimated shake flask CO(2) evolution test was used to study the biodegradability of 14 commercial phthalate esters that are commonly used as plasticizers. Both CO(2) evolution (ultimate biodegradation) and loss of parent phthalate esters (primary biodegradation) were measured. With only a few exceptions, primary ...

journal_title：Applied and environmental microbiology

authors： Sugatt RH,O'grady DP,Banerjee S,Howard PH,Gledhill WE

更新日期：1984-04-01 00:00:00

abstract：:Rubber-degrading activity was found in the extracellular culture medium of Xanthomonas sp. strain 35Y which was grown on natural rubber latex. Natural rubber in the latex state was degraded by the crude enzyme, and two fractions were separately observed by gel permeation chromatography of the reaction products. One fr...

journal_title：Applied and environmental microbiology

authors： Tsuchii A,Takeda K

更新日期：1990-01-01 00:00:00

abstract：:Although their exact function remains enigmatic, bifidobacteria are among the first colonizers of the newborn infant gut and further develop into abundant communities, notably in response to diet. Therefore, the transcriptional responses of bifidobacteria in rapidly processed fecal samples from young infants that were...

journal_title：Applied and environmental microbiology

authors： Klaassens ES,Boesten RJ,Haarman M,Knol J,Schuren FH,Vaughan EE,de Vos WM

更新日期：2009-05-01 00:00:00

abstract：:Sclerotinia sclerotiorum acidifies its ambient environment by producing oxalic acid. This production of oxalic acid during plant infection has been implicated as a primary determinant of pathogenicity in this and other phytopathogenic fungi. We found that ambient pH conditions affect multiple processes in S. sclerotio...

journal_title：Applied and environmental microbiology

authors： Rollins JA,Dickman MB

更新日期：2001-01-01 00:00:00

abstract：:An in-depth study of the continuous cell line designated BGM is described herein, and recommendations are made for standardizing cell culture and viral assay procedures. Based on data gathered from a survey of 58 laboratories using this cell line, a research plan was developed that included the study of growth media, ...

journal_title：Applied and environmental microbiology

authors： Dahling DR,Wright BA

更新日期：1986-04-01 00:00:00

abstract：:beta-D-Galactosidase (EC 3.2.1.23) was extracted from Streptococcus thermophilus grown in deproteinized cheese whey. Cultural conditions optimum for maximum enzyme production were pH 7.0, 40 degrees C, and 24 h. Proteose peptone (2.0%, wt/vol) and corn steep liquor (2.8%, wt/vol) were highly stimulatory, increasing th...

journal_title：Applied and environmental microbiology

authors： Rao MV,Dutta SM

更新日期：1977-08-01 00:00:00