Abstract:
:Cyanuric acid, a metabolic intermediate in the degradation of many s-triazine compounds, is further metabolized by cyanuric acid hydrolase. Cyanuric acid also accumulates in swimming pools due to the breakdown of the sanitizing agents di- and trichloroisocyanuric acid. Structurally stable cyanuric acid hydrolases are being considered for usage in pool water remediation. In this study, cyanuric acid hydrolase from the thermophile Moorella thermoacetica ATCC 39073 was cloned, expressed in Escherichia coli, and purified to homogeneity. The recombinant enzyme was found to have a broader temperature range and greater stability, at both elevated and low temperatures, than previously described cyanuric acid hydrolases. The enzyme had a narrow substrate specificity, acting only on cyanuric acid and N-methylisocyanuric acid. The M. thermoacetica enzyme did not require metals or other discernible cofactors for activity. Cyanuric acid hydrolase from M. thermoacetica is the most promising enzyme to use for cyanuric acid remediation applications.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Li Q,Seffernick JL,Sadowsky MJ,Wackett LPdoi
10.1128/AEM.01605-09subject
Has Abstractpub_date
2009-11-01 00:00:00pages
6986-91issue
22eissn
0099-2240issn
1098-5336pii
AEM.01605-09journal_volume
75pub_type
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