Abstract:
:An in-depth study of the continuous cell line designated BGM is described herein, and recommendations are made for standardizing cell culture and viral assay procedures. Based on data gathered from a survey of 58 laboratories using this cell line, a research plan was developed that included the study of growth media, sera, NaHCO3 levels, culture bottles, cell concentration, overlay media, agar, virus infection conditions, and cell-dissociating agents. Additionally, a comparative virus isolation study with BGM cells and nine other cell types was conducted with 37 sewage samples collected from nine different geographic areas. The results of the study indicated that the BGM cell line is superior for virus isolation when compared with the other cell types and that certain media and additives tend to increase BGM cell sensitivity to a specific group of viruses. A standardized procedure for cultivation of BGM cells is described which provides a more effective enterovirus assay system.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Dahling DR,Wright BAdoi
10.1128/AEM.51.4.790-812.1986subject
Has Abstractpub_date
1986-04-01 00:00:00pages
790-812issue
4eissn
0099-2240issn
1098-5336journal_volume
51pub_type
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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