Enumeration and isolation of lactate-utilizing bacteria from the rumen of sheep.

abstract：:A simplified X-ray film method for detecting bacterial production of Hgo by Escherichia coli was developed. There was excellent agreement of results between this X-ray film method and radioactive mercury volatilization assays. Although not quantitative, the X-ray film method has the advantages of being rapid, simple t...

journal_title：Applied and environmental microbiology

authors： Nakamura K,Nakahara H

更新日期：1988-11-01 00:00:00

abstract：:Bacterial plasmids are fragments of extrachromosomal double-stranded DNA that can contain a variety of genes that are beneficial to the host organism, like those responsible for antimicrobial resistance. The objective of this study was to characterize a collection of 437 Salmonella enterica isolates from different ani...

journal_title：Applied and environmental microbiology

authors： Lindsey RL,Fedorka-Cray PJ,Frye JG,Meinersmann RJ

更新日期：2009-04-01 00:00:00

abstract：:Medium-chain-length (mcl) poly(3-hydroxyalkanoates) (PHAs) are storage polymers that are produced from various substrates and accumulate in Pseudomonas strains belonging to rRNA homology group I. In experiments aimed at increasing PHA production in Pseudomonas strains, we generated an mcl PHA-overproducing mutant of P...

journal_title：Applied and environmental microbiology

authors： Klinke S,Dauner M,Scott G,Kessler B,Witholt B

更新日期：2000-03-01 00:00:00

abstract：:The ever-increasing industrial demand for biocatalysis necessitates innovations in the preparation and stabilization of biocatalysts. In this study, we demonstrated that beta-galactosidase (beta-Gal) displayed on Bacillus spores by fusion to the spore coat proteins (CotG) may be used as a whole-cell immobilized biocat...

journal_title：Applied and environmental microbiology

authors： Kwon SJ,Jung HC,Pan JG

更新日期：2007-04-01 00:00:00

abstract：:Suppression of soilborne disease by fluorescent pseudomonads may be inconsistent. Inefficient root colonization by the introduced bacteria is often responsible for this inconsistency. To better understand the bacterial traits involved in root colonization, the effect of two plant species, flax (Linum usitatissinum L.)...

journal_title：Applied and environmental microbiology

authors： Lemanceau P,Corberand T,Gardan L,Latour X,Laguerre G,Boeufgras J,Alabouvette C

更新日期：1995-03-01 00:00:00

abstract：:Monitoring cell growth and measuring physical features of food-borne pathogenic bacteria are important for better understanding the conditions under which these organisms survive and proliferate. To address this challenge, buoyant masses of live and dead Escherichia coli O157:H7 and Listeria innocua were measured usin...

journal_title：Applied and environmental microbiology

authors： Lewis CL,Craig CC,Senecal AG

更新日期：2014-06-01 00:00:00

abstract：:The organization and species composition of bacterial trophic groups associated with lactose biomethanation were investigated in a whey-processing chemostat by enumeration, isolation, and general characterization studies. The bacteria were spatially organized as free-living forms and as self-immobilized forms appearin...

journal_title：Applied and environmental microbiology

authors： Chartrain M,Zeikus JG

更新日期：1986-01-01 00:00:00

abstract：:The use of bioluminescence as a sensitive marker for detection of Pseudomonas spp. in the rhizosphere was investigated. Continuous expression of the luxCDABE genes, required for bioluminescence, was not detectable in the rhizosphere. However, when either a naphthalene-inducible luxCDABE construct or a constitutive lux...

journal_title：Applied and environmental microbiology

authors： de Weger LA,Dunbar P,Mahafee WF,Lugtenberg BJ,Sayler GS

更新日期：1991-12-01 00:00:00

abstract：:The aim of this study was to compare the antagonistic properties of Lactobacillus casei GG exerted in vitro against Salmonella typhimurium C5 in a cellular model, cultured enterocyte-like Caco-2 cells, to those exerted in vivo in an animal model, C3H/He/Oujco mice. Our results show that a 1-h contact between the invad...

journal_title：Applied and environmental microbiology

authors： Hudault S,Liévin V,Bernet-Camard MF,Servin AL

更新日期：1997-02-01 00:00:00

abstract：:The structure and stability of the dominant lactic acid bacterium population were assessed during wheat flour sourdough type I propagation by using singly nine strains of Lactobacillus sanfranciscensis. Under back-slopping propagation with wheat flour type 0 F114, cell numbers of presumptive lactic acid bacteria varie...

journal_title：Applied and environmental microbiology

authors： Siragusa S,Di Cagno R,Ercolini D,Minervini F,Gobbetti M,De Angelis M

更新日期：2009-02-01 00:00:00

abstract：:We devised a new method (the dorayaki method) using marine agar under in situ pressures to isolate barophilic bacteria from the intestinal contents of three deep-sea fishes (two Coryphaenoides yaquinae samples and one Ilyophis sp. sample) retrieved from depths of 4,700 to 6,100 m in the Northwest Pacific Ocean. All 10...

journal_title：Applied and environmental microbiology

authors： Nakayama A,Yano Y,Yoshida K

更新日期：1994-11-01 00:00:00

abstract：:Nitrogen-fixing symbiosis between bacteria and the tree legume mesquite (Prosopis glandulosa) is important for the maintenance of many desert ecosystems. Genes essential for nodulation and for extending the host range to mesquite were isolated from cosmid libraries of Rhizobium (mesquite) sp. strain HW17b and Bradyrhi...

journal_title：Applied and environmental microbiology

authors： Thomas PM,Golly KF,Virginia RA,Zyskind JW

更新日期：1995-09-01 00:00:00

abstract：:Medicinal plants are valuable resources of natural antimicrobial materials. A novel small protein with antimicrobial activities, designated LJAMP1, was purified from the seeds of a medicinal herb, motherwort (Leonurus japonicus Houtt). LJAMP1 is a heat-stable protein with a molecular mass of 7.8 kDa and a determined i...

journal_title：Applied and environmental microbiology

authors： Yang X,Xiao Y,Wang X,Pei Y

更新日期：2007-02-01 00:00:00

abstract：:Helaeomyia petrolei larvae isolated from the asphalt seeps of Rancho La Brea in Los Angeles, Calif., were examined for microbial gut contents. Standard counts on Luria-Bertani, MacConkey, and blood agar plates indicated ca. 2 x 10(5) heterotrophic bacteria per larva. The culturable bacteria represented 15 to 20% of th...

journal_title：Applied and environmental microbiology

authors： Kadavy DR,Plantz B,Shaw CA,Myatt J,Kokjohn TA,Nickerson KW

更新日期：1999-04-01 00:00:00

abstract：:Aromatic hydroxylations are important bacterial metabolic processes but are difficult to perform using traditional chemical synthesis, so to use a biological catalyst to convert the priority pollutant benzene into industrially relevant intermediates, benzene oxidation was investigated. It was discovered that toluene 4...

journal_title：Applied and environmental microbiology

authors： Tao Y,Fishman A,Bentley WE,Wood TK

更新日期：2004-07-01 00:00:00

abstract：:An unknown member of the family Pasteurellaceae was repeatedly isolated from 20- to 24-week-old pigs with severe pulmonary lesions reared on the same farm in Victoria, Australia. The etiological diagnosis of the disease was inconclusive. The complete genome sequence analysis of one strain, 15-184, revealed some phylog...

journal_title：Applied and environmental microbiology

authors： Watt AE,Browning GF,Legione AR,Bushell RN,Stent A,Cutler RS,Young ND,Marenda MS

更新日期：2018-05-17 00:00:00

abstract：:Bacteria in the genus Rickettsiella (Coxiellaceae), which are mainly known as arthropod pathogens, are emerging as excellent models to study transitions between mutualism and pathogenicity. The current report characterizes a novel Rickettsiella found in the leafhopper Orosius albicinctus (Hemiptera: Cicadellidae), a m...

journal_title：Applied and environmental microbiology

authors： Iasur-Kruh L,Weintraub PG,Mozes-Daube N,Robinson WE,Perlman SJ,Zchori-Fein E

更新日期：2013-07-01 00:00:00

abstract：:Despite the importance of human noroviruses (NoVs) in public health, little information concerning the effectiveness of ozone against NoVs is available. We determined the efficacy of ozone disinfection using murine norovirus (MNV) as a surrogate of human NoV. MNV in ozone demand-free buffer was exposed to a predetermi...

journal_title：Applied and environmental microbiology

authors： Lim MY,Kim JM,Lee JE,Ko G

更新日期：2010-02-01 00:00:00

abstract：:Human norovirus (NoV) is the leading cause of foodborne disease in the United States, and epidemiological studies have shown that fresh produce is one of the major vehicles for the transmission of human NoV. However, the mechanisms of norovirus contamination and persistence in fresh produce are poorly understood. The ...

journal_title：Applied and environmental microbiology

authors： DiCaprio E,Culbertson D,Li J

更新日期：2015-04-01 00:00:00

abstract：:In a field study of 29 dairy farms, Pseudomonas aeruginosa was isolated more frequently (P = 0.05) from milking parlor udder wash water systems containing iodophor germicides than from those with no germicide. Most available iodine (AI2) concentrations were below the recommended level of 25 ppm (25 microgram/ml). Rubb...

journal_title：Applied and environmental microbiology

authors： Hicks CR,Eberhart RJ,Erskine RJ,Spencer SB

更新日期：1991-02-01 00:00:00

abstract：:Sodium gradients in cyanobacteria play an important role in energy storage under photoautotrophic conditions but have not been well studied during autofermentative metabolism under the dark, anoxic conditions widely used to produce precursors to fuels. Here we demonstrate significant stress-induced acceleration of aut...

journal_title：Applied and environmental microbiology

authors： Carrieri D,Ananyev G,Lenz O,Bryant DA,Dismukes GC

更新日期：2011-10-01 00:00:00

abstract：:Detection of psychrotrophic strains (those able to grow at or below 7 degreesC) of the Bacillus cereus group (Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides) in food products is at present extremely slow with conventional microbiology. This is due to an inability to discriminate these cold-adapted stra...

journal_title：Applied and environmental microbiology

authors： Francis KP,Mayr R,von Stetten F,Stewart GS,Scherer S

更新日期：1998-09-01 00:00:00

abstract：:This was the first molecular study of the bacterial flora of the sheep scab mite (Psoroptes ovis). A sequence analysis of genes coding for 16S rRNA revealed that Serratia marcescens and bacteria closely related to Staphylococcus intermedius or Staphylococcus chromogens and Alloiococcus otitidis were present. These bac...

journal_title：Applied and environmental microbiology

authors： Hogg JC,Lehane MJ

更新日期：1999-09-01 00:00:00

abstract：:Several probiotic strains of Bifidobacterium animalis subsp. lactis are widely supplemented into food products and dietary supplements due to their documented health benefits and ability to survive within the mammalian gastrointestinal tract and acidified dairy products. The strain specificity of these characteristics...

journal_title：Applied and environmental microbiology

authors： Briczinski EP,Loquasto JR,Barrangou R,Dudley EG,Roberts AM,Roberts RF

更新日期：2009-12-01 00:00:00

abstract：:We developed a PCR detection method that selectively recognizes a single biological control agent and demonstrated that universally primed PCR (UP-PCR) can identify strain-specific markers. Antagonistic strains of Clonostachys rosea (syn. Gliocladium roseum) were screened by UP-PCR, and a strain-specific marker was id...

journal_title：Applied and environmental microbiology

authors： Bulat SA,Lübeck M,Alekhina IA,Jensen DF,Knudsen IM,Lübeck PS

更新日期：2000-11-01 00:00:00

abstract：:To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) ...

journal_title：Applied and environmental microbiology

authors： Villarreal DM,Phillips CL,Kelley AM,Villarreal S,Villaloboz A,Hernandez P,Olson JS,Henderson DP

更新日期：2008-09-01 00:00:00

abstract：:Profiles of dissolved O(2) and methane with increasing depth were generated for Lake Washington sediment, which suggested the zone of methane oxidation is limited to the top 0.8 cm of the sediment. Methane oxidation potentials were measured for 0.5-cm layers down to 1.5 cm and found to be relatively constant at 270 to...

journal_title：Applied and environmental microbiology

authors： Auman AJ,Stolyar S,Costello AM,Lidstrom ME

更新日期：2000-12-01 00:00:00

abstract：:The genome of Dehalococcoides ethenogenes strain 195, an anaerobic dehalorespiring bacterium, contains 18 copies of putative reductive dehalogenase genes, including the well-characterized tceA gene, whose gene product functions as the key enzyme in the environmentally important dehalorespiration process. The genome of...

journal_title：Applied and environmental microbiology

authors： Regeard C,Maillard J,Dufraigne C,Deschavanne P,Holliger C

更新日期：2005-06-01 00:00:00

abstract：:Comparative genomic hybridizations have been used to examine genetic relationships among bacteria. The microarrays used in these experiments may have open reading frames from one or more reference strains (whole-genome microarrays), or they may be composed of random DNA fragments from a large number of strains (mixed-...

journal_title：Applied and environmental microbiology

authors： Wan Y,Broschat SL,Call DR

更新日期：2007-03-01 00:00:00

abstract：:Caldalkalibacillus thermarum strain TA2.A1 grew in pH-controlled batch culture containing a fermentable growth substrate (i.e., sucrose) from pH 7.5 to 10.0 with no significant change in the specific growth rate, suggesting that this bacterium was a facultative alkaliphile. However, when strain TA2.A1 was grown on a n...

journal_title：Applied and environmental microbiology

authors： McMillan DG,Keis S,Berney M,Cook GM

更新日期：2009-12-01 00:00:00