Validation of mixed-genome microarrays as a method for genetic discrimination.

abstract：:The survival of microorganisms can be predicted through the use of active biological containment systems. We have constructed contained Pseudomonas putida strains that degrade alkylbenzoates. The modified strain carries a fusion of the Plac promoter to the gef gene, which encodes a killing protein. Expression from Pla...

journal_title：Applied and environmental microbiology

authors： Ronchel MC,Ramos C,Jensen LB,Molin S,Ramos JL

更新日期：1995-08-01 00:00:00

abstract：:The pathways for degradation of aromatic hydrocarbons are constantly modified by a variety of genetic mechanisms. Genetic studies carried out with Pseudomonas stutzeri OX1 suggested that the tou operon coding for toluene o-xylene monooxygenase (ToMO) was recently recruited into a preexisting pathway that already posse...

journal_title：Applied and environmental microbiology

authors： Cafaro V,Notomista E,Capasso P,Di Donato A

更新日期：2005-08-01 00:00:00

abstract：:After the first isolation of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in 2003, this MRSA variant quickly became the predominant MRSA obtained from humans as part of the Dutch national MRSA surveillance. Previous studies have suggested that human-to-human transmission of LA-MRSA, compa...

journal_title：Applied and environmental microbiology

authors： Bosch T,Verkade E,van Luit M,Landman F,Kluytmans J,Schouls LM

更新日期：2015-01-01 00:00:00

abstract：:2-Chloro-4,6-diamino-s-triazine (CAAT) is a metabolite of atrazine biodegradation in soils. Atrazine chlorohydrolase (AtzA) catalyzes the dechlorination of atrazine but is unreactive with CAAT. In this study, melamine deaminase (TriA), which is 98% identical to AtzA, catalyzed deamination of CAAT to produce 2-chloro-4...

journal_title：Applied and environmental microbiology

authors： Seffernick JL,Shapir N,Schoeb M,Johnson G,Sadowsky MJ,Wackett LP

更新日期：2002-09-01 00:00:00

abstract：:The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...

journal_title：Applied and environmental microbiology

authors： Staley BF,de Los Reyes FL 3rd,Barlaz MA

更新日期：2011-04-01 00:00:00

abstract：:The response of methanogenesis and sulfate reduction to trimethylamine, choline, and glycine betaine was examined in surface sediments from the intertidal region of Lowes Cove, Maine. Addition of these substrates markedly stimulated methanogenesis in the presence of active sulfate reduction, whereas addition of other ...

journal_title：Applied and environmental microbiology

authors： King GM

更新日期：1984-10-01 00:00:00

abstract：:Low-molecular-weight (LMW) RNA molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in Spain. Since LMW RNA profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from Spanish soil...

journal_title：Applied and environmental microbiology

authors： Velázquez E,Martínez-Romero E,Rodríguez-Navarro DN,Trujillo ME,Daza A,Mateos PF,Martínez-Molina E,van Berkum P

更新日期：2001-02-01 00:00:00

abstract：:The human oral cavity is a complex ecosystem, and the alterations in salivary microbial communities are associated with both oral and nonoral diseases. The Mediterranean diet (MD) is a healthy dietary pattern useful for both prevention and treatment of several diseases. To further explore the effects of the MD on huma...

journal_title：Applied and environmental microbiology

authors： Laiola M,De Filippis F,Vitaglione P,Ercolini D

更新日期：2020-06-02 00:00:00

abstract：:A simple synthetic liquid medium containing a single amino acid, glucose, salts, trace metals, and thiamine was developed for cultivation of Cercospora rosicola Passerini. Thiamine was shown to be important to growth. Culture of C. rosicola Passerini in a chemically defined medium makes possible studies of (+)-abscisi...

journal_title：Applied and environmental microbiology

authors： Norman SM,Maier VP,Echols LC

更新日期：1981-01-01 00:00:00

abstract：:Through use of commercially available DnaK proteins and anti-DnaK monoclonal antibodies, a competitive enzyme-linked immunosorbent assay was developed to quantify this heat shock protein in Escherichia coli ATCC 25922 subjected to various heating regimens. For a given process lethality (F(70)(10) of 1, 3, and 5 min), ...

journal_title：Applied and environmental microbiology

authors： Seyer K,Lessard M,Piette G,Lacroix M,Saucier L

更新日期：2003-06-01 00:00:00

abstract：:A unique, coleopteran-active protein, termed eCry3.1Ab, was generated following variable-region exchange of a Bacillus thuringiensis lepidopteran-active protein, Cry1Ab, with a Cry3A region. Our results support the hypothesis that this variable-region exchange is responsible for imparting strong bioactivity against th...

journal_title：Applied and environmental microbiology

authors： Walters FS,deFontes CM,Hart H,Warren GW,Chen JS

更新日期：2010-05-01 00:00:00

abstract：:Three common food pathogenic microorganisms were exposed to treatments simulating those used in food processing. Treated cell suspensions were then analyzed for reduction in growth by plate counting. Flow cytometry (FCM) and fluorescence-activated cell sorting (FACS) were carried out on treated cells stained for membr...

journal_title：Applied and environmental microbiology

authors： Kennedy D,Cronin UP,Wilkinson MG

更新日期：2011-07-01 00:00:00

abstract：:The production of the H(2)O(2)-generating enzyme pyranose oxidase (POD) (EC 1.1.3.10) (synonym, glucose 2-oxidase), two ligninolytic peroxidases, and laccase in wood decayed by three white rot fungi was investigated by correlated biochemical, immunological, and transmission electron microscopic techniques. Enzyme acti...

journal_title：Applied and environmental microbiology

authors： Daniel G,Volc J,Kubatova E

更新日期：1994-07-01 00:00:00

abstract：:Fresh produce is known to carry nonpathogenic epiphytic microorganisms. During agricultural production and harvesting, leafy greens can become contaminated with antibiotic-resistant pathogens or commensals from animal and human sources. As lettuce does not undergo any inactivation or preservation treatment during proc...

journal_title：Applied and environmental microbiology

authors： Holvoet K,Sampers I,Callens B,Dewulf J,Uyttendaele M

更新日期：2013-11-01 00:00:00

abstract：:The intracellular pathogens Legionella micdadei and Legionella pneumophila are the two most common Legionella species that cause Legionnaires' disease. Intracellular replication within pulmonary cells is the hallmark of Legionnaires' disease. In the environment, legionellae are parasites of protozoans, and intracellul...

journal_title：Applied and environmental microbiology

authors： Abu Kwaik Y,Venkataraman C,Harb OS,Gao LY

更新日期：1998-09-01 00:00:00

abstract：:Endotoxin exposure occurs in homes and occupational environments and is known to cause adverse health effects. In order to compare results from different studies and establish standards, airborne endotoxin exposures should be assessed using standardized methods. Although the European Committee for Standardization (CEN...

journal_title：Applied and environmental microbiology

authors： Spaan S,Heederik DJ,Thorne PS,Wouters IM

更新日期：2007-10-01 00:00:00

abstract：:Lysine iron agar, modified by the addition of bile salts, novobiocin, lactose, and sucrose, is a valuable plating medium for the isolation of Salmonella, including H2S-negative strains. ...

journal_title：Applied and environmental microbiology

authors： Rappold H,Bolderdijk RF

更新日期：1979-07-01 00:00:00

abstract：:The present work describes the development of a superior strain of Corynebacterium glutamicum for diaminopentane (cadaverine) production aimed at the identification and deletion of the underlying unknown N-acetyldiaminopentane pathway. This acetylated product variant, recently discovered, is a highly undesired by-prod...

journal_title：Applied and environmental microbiology

authors： Kind S,Jeong WK,Schröder H,Zelder O,Wittmann C

更新日期：2010-08-01 00:00:00

abstract：:Two isolates of Neodiprion sertifer (Geoffr.) nuclear polyhedrosis virus from Britain and North America were compared using three biochemical techniques. Alkaline protease assays of polyhedra revealed the presence of endogenous enzyme activity in the British isolate but not in the North American isolate. Sodium dodecy...

journal_title：Applied and environmental microbiology

authors： Brown DA

更新日期：1982-01-01 00:00:00

abstract：:Seventy-two Listeria monocytogenes isolates originating from 10 different fish products of 12 producers and 47 isolates from human listeriosis cases were typed by serotyping and multilocus enzyme electrophoresis. Seventy-five of these isolates were further subtyped by restriction analysis of genomic DNA with the enzym...

journal_title：Applied and environmental microbiology

authors： Boerlin P,Boerlin-Petzold F,Bannerman E,Bille J,Jemmi T

更新日期：1997-04-01 00:00:00

abstract：:Bacterial rhizosphere colonization is critical for phytobeneficial rhizobacteria such as phenazine-producing Pseudomonas spp. To better understand this colonization process, potential metabolic and genomic determinants required for rhizosphere colonization were identified using a collection of 60 phenazine-producing P...

journal_title：Applied and environmental microbiology

authors： Zboralski A,Biessy A,Savoie MC,Novinscak A,Filion M

更新日期：2020-02-03 00:00:00

abstract：:Washed cell suspensions of Desulfovibrio vulgaris rapidly reduced Cr(VI) to Cr(III) with H(2) as the electron donor. The c(3) cytochrome from this organism functioned as a Cr(VI) reductase. D. vulgaris may have advantages over previously described Cr(VI) reducers for the bioremediation of Cr(VI)-contaminated waters. ...

journal_title：Applied and environmental microbiology

authors： Lovley DR,Phillips EJ

更新日期：1994-02-01 00:00:00

abstract：:In bacteriophage (phage) therapy against Gram-positive bacteria, such as Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis, members of a genus of SPO1-like viruses are typically employed because of their extreme virulence and broad host spectrum. Phage φEF24C, which is a SPO1-like virus infectin...

journal_title：Applied and environmental microbiology

authors： Uchiyama J,Takemura I,Hayashi I,Matsuzaki S,Satoh M,Ujihara T,Murakami M,Imajoh M,Sugai M,Daibata M

更新日期：2011-01-01 00:00:00

abstract：:The susceptibility of the Iberian ibex (Capra pyrenaica) to Mycoplasma conjunctivae ocular infection and the changes in their interaction over time were studied in terms of clinical outcome, molecular detection, and IgG immune response in a captive population that underwent a severe infectious keratoconjunctivitis (IK...

journal_title：Applied and environmental microbiology

authors： Fernández-Aguilar X,Cabezón O,Granados JE,Frey J,Serrano E,Velarde R,Cano-Manuel FJ,Mentaberre G,Ráez-Bravo A,Fandos P,López-Olvera JR

更新日期：2017-07-17 00:00:00

abstract：:DNA fingerprinting and molecular characterization showed that the tetracycline-resistant Staphylococcus aureus population of a South African poultry processing plant comprised one or possibly several tet(K)-containing endemic clones that contaminated chicken and machinery surfaces at all sampled processing stages. The...

journal_title：Applied and environmental microbiology

authors： Huys G,D'Haene K,Van Eldere J,von Holy A,Swings J

更新日期：2005-01-01 00:00:00

abstract：:The aerobic intestinal microflora of 2-week-old herring (Clupea harengus) larvae was characterized by using conventional microbiological methods and electron microscopy. Larvae were hatched and kept in filtered seawater or in seawater with penicillin and streptomycin. The gastrointestinal tract of herring larvae is es...

journal_title：Applied and environmental microbiology

authors： Hansen GH,Strøm E,Olafsen JA

更新日期：1992-02-01 00:00:00

abstract：:We describe the stabilization by pressure of enzymes, including a hydrogenase from Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen. This is the first published report of proteins from thermophiles being stabilized by pressure. Inactivation studies of partially purified hydrogenases from an extr...

journal_title：Applied and environmental microbiology

authors： Hei DJ,Clark DS

更新日期：1994-03-01 00:00:00

abstract：:Laboratory strains of Saccharomyces cerevisiae have been widely used as a model for studying eukaryotic cells and mapping the molecular mechanisms of many different human diseases. Industrial wine yeasts, on the other hand, have been selected on the basis of their adaptation to stringent environmental conditions and t...

journal_title：Applied and environmental microbiology

authors： Pizarro FJ,Jewett MC,Nielsen J,Agosin E

更新日期：2008-10-01 00:00:00

abstract：:The soil isolate Cellvibrio mixtus UQM2294 degraded a variety of polysaccharides including microcrystalline cellulose. Among 6,000 cosmid clones carrying C. mixtus DNA, constructed in Escherichia coli with pHC79, 50 expressed the ability to degrade one or more of the following substrates: carboxymethyl cellulose, chit...

journal_title：Applied and environmental microbiology

authors： Wynne EC,Pemberton JM

更新日期：1986-12-01 00:00:00

abstract：:The competitive inhibition of fructokinase by glucose has been proposed as the mechanism by which Zymomonas mobilis preferentially consumes glucose from mixtures of glucose and fructose and accumulates fructose when growing on sucrose. In this study, incorporation of radioactive fructose into biomass was used as a mea...

journal_title：Applied and environmental microbiology

authors： Parker C,Peekhaus N,Zhang X,Conway T

更新日期：1997-09-01 00:00:00