Regiospecificity of two multicomponent monooxygenases from Pseudomonas stutzeri OX1: molecular basis for catabolic adaptation of this microorganism to methylated aromatic compounds.

Abstract:

:The pathways for degradation of aromatic hydrocarbons are constantly modified by a variety of genetic mechanisms. Genetic studies carried out with Pseudomonas stutzeri OX1 suggested that the tou operon coding for toluene o-xylene monooxygenase (ToMO) was recently recruited into a preexisting pathway that already possessed the ph operon coding for phenol hydroxylase (PH). This apparently resulted in a redundancy of enzymatic activities, because both enzymes are able to hydroxylate (methyl)benzenes to (methyl)catechols via the intermediate production of (methyl)phenols. We investigated the kinetics and regioselectivity of toluene and o-xylene oxidation using Escherichia coli cells expressing ToMO and PH complexes. Our data indicate that in the recombinant system the enzymes act sequentially and that their catalytic efficiency and regioselectivity optimize the degradation of toluene and o-xylene, both of which are growth substrates. The main product of toluene oxidation by ToMO is p-cresol, the best substrate for PH, which catalyzes its transformation to 4-methylcatechol. The sequential action of the two enzymes on o-xylene leads, via the intermediate 3,4-dimethylphenol, to the exclusive production of 3,4-dimethylcatechol, the only dimethylcatechol isomer that can serve as a carbon and energy source after further metabolic processing. Moreover, our data strongly support a metabolic explanation for the acquisition of the ToMO operon by P. stutzeri OX1. It is possible that using the two enzymes in a concerted fashion confers on the strain a selective advantage based on the ability of the microorganism to optimize the efficiency of the use of nonhydroxylated aromatic hydrocarbons, such as benzene, toluene, and o-xylene.

journal_name

Appl Environ Microbiol

authors

Cafaro V,Notomista E,Capasso P,Di Donato A

doi

10.1128/AEM.71.8.4736-4743.2005

keywords:

subject

Has Abstract

pub_date

2005-08-01 00:00:00

pages

4736-43

issue

8

eissn

0099-2240

issn

1098-5336

pii

71/8/4736

journal_volume

71

pub_type

杂志文章
  • Differential transmission of the genospecies of Borrelia burgdorferi sensu lato by game birds and small rodents in England.

    abstract::The genetic diversity of Borrelia burgdorferi sensu lato was assessed in a focus of Lyme borreliosis in southern Britain dominated by game birds. Ticks, rodents, and pheasants were analyzed for spirochete infections by PCR-targeting the 23S-5S rRNA genes, followed by genotyping by the reverse line blot method. In ques...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.4.1169-1174.1998

    authors: Kurtenbach K,Peacey M,Rijpkema SG,Hoodless AN,Nuttall PA,Randolph SE

    更新日期:1998-04-01 00:00:00

  • Toxic and enzymatic activities of Vibrio vulnificus biotype 2 with respect to host specificity.

    abstract::In this work, the enzymatic activities of selected strains of biotypes 1 and 2 of Vabrio vulnificus were analyzed by using conventional methods and the API ZYM system. The toxic activities of extracellular products (ECPs) were further evaluated by in vitro and in vivo experiments. The ECPs of both biotypes (i) showed ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.7.2331-2337.1996

    authors: Biosca EG,Amaro C

    更新日期:1996-07-01 00:00:00

  • Fate of the nitrilotriacetic acid-Fe(III) complex during photodegradation and biodegradation by Rhodococcus rhodochrous.

    abstract::Aminopolycarboxylic acids are ubiquitous in natural waters and wastewaters. They have the ability to form very stable water-soluble complexes with many metallic di- or trivalent ions. The iron complex nitrilotriacetic acid-Fe(III) (FeNTA) has been previously shown to increase drastically the rate of photo- and biodegr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00537-08

    authors: Bunescu A,Besse-Hoggan P,Sancelme M,Mailhot G,Delort AM

    更新日期:2008-10-01 00:00:00

  • Evidence for Multidrug Resistance in Nonpathogenic Mycoplasma Species Isolated from South African Poultry.

    abstract::One hundred seventy-eight mycoplasma strains isolated from South African poultry flocks between 2003 and 2015 were identified by full-genome sequencing and phylogenetic analysis of the 16S rRNA gene and were classified as follows: Mycoplasma gallisepticum (25%), M. gallinarum (25%), M. gallinaceum, (23%), M. pullorum ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01660-18

    authors: Beylefeld A,Wambulawaye P,Bwala DG,Gouws JJ,Lukhele OM,Wandrag DBR,Abolnik C

    更新日期:2018-10-17 00:00:00

  • Marine cyanophages demonstrate biogeographic patterns throughout the global ocean.

    abstract::Myoviruses and podoviruses that infect cyanobacteria are the two major groups of marine cyanophages, but little is known of how their phylogenetic lineages are distributed in different habitats. In this study, we analyzed the phylogenetic relationships of cyanopodoviruses and cyanomyoviruses based on the existing geno...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02483-14

    authors: Huang S,Zhang S,Jiao N,Chen F

    更新日期:2015-01-01 00:00:00

  • Genetic population structure of the soil bacterium Myxococcus xanthus at the centimeter scale.

    abstract::Myxococcus xanthus is a gram-negative soil bacterium best known for its remarkable life history of social swarming, social predation, and multicellular fruiting body formation. Very little is known about genetic diversity within this species or how social strategies might vary among neighboring strains at small spatia...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.5.3615-3625.2006

    authors: Vos M,Velicer GJ

    更新日期:2006-05-01 00:00:00

  • Sorption and metabolism of metolachlor by a bacterial community.

    abstract::A stable bacterial community absorbed and transformed the herbicide metolachlor [2-chloro-N-(2-ethyl- 6-methylphenyl)-N-(2-methoxy-1-methylethyl)-acetamide] from a liquid medium. About 80% of the added ring-[U-C]metolachlor (50 mug/ml) disappeared from the medium and accumulated inside the cells. The ratio of cellular...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.3.733-740.1989

    authors: Liu SY,Zheng Z,Zhang R,Bollag JM

    更新日期:1989-03-01 00:00:00

  • Division of Listeria monocytogenes serovar 1/2a strains into two groups by PCR and restriction enzyme analysis.

    abstract::Altogether, 100 strains of Listeria monocytogenes serovar 1/2a isolated from humans, animals, food, and the environment were typed by a combination of PCR and restriction enzyme analysis (REA). A PCR product of 2,916 bp, containing the downstream end of the gene inlA (955 bp), the space between inlA and inlB (85 bp), ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.5.2054-2056.1999

    authors: Unnerstad H,Nilsson I,Ericsson H,Danielsson-Tham ML,Bille J,Bannerman E,Tham W

    更新日期:1999-05-01 00:00:00

  • Organic solvent tolerance and antibiotic resistance increased by overexpression of marA in Escherichia coli.

    abstract::We previously reported that overexpression of the soxS or robA gene causes in several Escherichia coli strains the acquisition of higher organic solvent tolerance and also increased resistance to a number of antibiotics (H. Nakajima, K. Kobayashi, M. Kobayashi, H. Asako, and R. Aono, Appl. Environ. Microbiol. 61:2302-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.4.1428-1433.1997

    authors: Asako H,Nakajima H,Kobayashi K,Kobayashi M,Aono R

    更新日期:1997-04-01 00:00:00

  • Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate.

    abstract::Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00278-07

    authors: Iwamoto S,Motomura K,Shinoda Y,Urata M,Kato J,Takiguchi N,Ohtake H,Hirota R,Kuroda A

    更新日期:2007-09-01 00:00:00

  • Comparison of methods for evaluation of the bactericidal activity of copper-sputtered surfaces against methicillin-resistant Staphylococcus aureus.

    abstract::Bacteria can survive on hospital textiles and surfaces, from which they can be disseminated, representing a source of health care-associated infections (HCAIs). Surfaces containing copper (Cu), which is known for its bactericidal properties, could be an efficient way to lower the burden of potential pathogens. The ant...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02266-12

    authors: Rio L,Kusiak-Nejman E,Kiwi J,Bétrisey B,Pulgarin C,Trampuz A,Bizzini A

    更新日期:2012-12-01 00:00:00

  • AcrR and Rex Control Mannitol and Sorbitol Utilization through Their Cross-Regulation of Aldehyde-Alcohol Dehydrogenase (AdhE) in Lactobacillus plantarum.

    abstract::Lactobacillus plantarum is a versatile bacterium that occupies a wide range of environmental niches. In this study, we found that a bifunctional aldehyde-alcohol dehydrogenase-encoding gene, adhE, was responsible for L. plantarum being able to utilize mannitol and sorbitol through cross-regulation by two DNA-binding r...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02035-18

    authors: Yang X,Teng K,Su R,Li L,Zhang T,Fan K,Zhang J,Zhong J

    更新日期:2019-02-06 00:00:00

  • Corrinoid-Dependent Methyl Transfer Reactions Are Involved in Methanol and 3,4-Dimethoxybenzoate Metabolism by Sporomusa ovata.

    abstract::Washed and air-oxidized proteins from Sporomusa ovata cleaved the C-O bond of methanol or methoxyaromatics and transferred the methyl to dl-tetrahydrofolate. The reactions strictly required a reductive activation by titanium citrate, catalytic amounts of ATP, and the addition of dl-tetrahydrofolate. Methylcorrinoid-co...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.9.3110-3116.1993

    authors: Stupperich E,Konle R

    更新日期:1993-09-01 00:00:00

  • Influence of incubation temperature on the microbial reductive dechlorination of 2,3,4,6-tetrachlorobiphenyl in two freshwater sediments.

    abstract::Volumn 62, no. 11, p. 4174, Abstract, lines 12 and 13: "para dechlorination was restricted from 18 to 34(deg)C" should read "para dechlorination was restricted to temperatures from 18 to 34(deg)C." [This corrects the article on p. 4174 in vol. 62.]. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:

    authors: Wu Q,Bedard DL,Wiegel J

    更新日期:1997-02-01 00:00:00

  • Influence of CO(2)-HCO(3) Levels and pH on Growth, Succinate Production, and Enzyme Activities of Anaerobiospirillum succiniciproducens.

    abstract::Growth and succinate versus lactate production from glucose by Anaerobiospirillum succiniciproducens was regulated by the level of available carbon dioxide and culture pH. At pH 7.2, the generation time was almost doubled and extensive amounts of lactate were formed in comparison with growth at pH 6.2. The succinate y...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.10.3013-3019.1991

    authors: Samuelov NS,Lamed R,Lowe S,Zeikus JG

    更新日期:1991-10-01 00:00:00

  • Antibacterial activity of Lactobacillus sake isolated from meat.

    abstract::A total of 221 strains of Lactobacillus isolated from meat and meat products were screened for antagonistic activities under conditions that eliminated the effects of organic acids and hydrogen peroxide. Nineteen strains of Lactobacillus sake, three strains of Lactobacillus plantarum, and one strain of Lactobacillus c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.8.1901-1906.1989

    authors: Schillinger U,Lücke FK

    更新日期:1989-08-01 00:00:00

  • Characterization of Pseudomonas spp. associated with spoilage of gilt-head sea bream stored under various conditions.

    abstract::The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (Sparus aurata) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 Pseudomonas strains isolat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.1.65-72.2002

    authors: Tryfinopoulou P,Tsakalidou E,Nychas GJ

    更新日期:2002-01-01 00:00:00

  • Chemical transformation of eremofortin C into PR toxin.

    abstract::The natural products of both eremofortin C (EC) and PR toxin are secondary metabolites of Penicillium roqueforti. Because the chemical structures of EC and PR toxin are closely related to each other and differ only by a hydroxyl functional group in EC and an aldehyde functional group in PR toxin at the C-12 position, ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.3.729-731.1985

    authors: Li SY,Chang SC,Wei RD

    更新日期:1985-09-01 00:00:00

  • Engineering trehalose synthesis in Lactococcus lactis for improved stress tolerance.

    abstract::Trehalose accumulation is a common cell defense strategy against a variety of stressful conditions. In particular, our team detected high levels of trehalose in Propionibacterium freudenreichii in response to acid stress, a result that led to the idea that endowing Lactococcus lactis with the capacity to synthesize tr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02922-10

    authors: Carvalho AL,Cardoso FS,Bohn A,Neves AR,Santos H

    更新日期:2011-06-01 00:00:00

  • Comparison of four membrane filter methods for fecal coliform enumeration in tropical waters.

    abstract::Four membrane filter methods for the enumeration of fecal coliforms were compared for accuracy, specificity, and recovery. Water samples were taken several times from 13 marine, 1 estuarine, and 4 freshwater sites around Puerto Rico, from pristine waters and waters receiving treated and untreated sewage and effluent f...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.12.2922-2928.1987

    authors: Santiago-Mercado J,Hazen TC

    更新日期:1987-12-01 00:00:00

  • Identification of Lactobacillus isolates from the gastrointestinal tract, silage, and yoghurt by 16S-23S rRNA gene intergenic spacer region sequence comparisons.

    abstract::Lactobacillus isolates were identified by PCR amplification and sequencing of the region between the 16S and 23S rRNA genes (spacer region). The sequences obtained from the isolates were compared to those of reference strains held in GenBank. A similarity of 97.5% or greater was considered to provide identification. T...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.9.4264-4267.1999

    authors: Tannock GW,Tilsala-Timisjarvi A,Rodtong S,Ng J,Munro K,Alatossava T

    更新日期:1999-09-01 00:00:00

  • High-Level Nickel Resistance in Alcaligenes xylosoxydans 31A and Alcaligenes eutrophus KTO2.

    abstract::Two new nickel-resistant strains of Alcaligenes species were selected from a large number (about 400) of strains isolated from ecosystems polluted by heavy metals and were studied on the physiological and molecular level. Alcaligenes xylosoxydans 31A is a heterotrophic bacterium, and Alcaligenes eutrophus KTO2 is an a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.11.3301-3309.1991

    authors: Schmidt T,Stoppel RD,Schlegel HG

    更新日期:1991-11-01 00:00:00

  • Production and Purification of Thermostable Amylase and Protease of Thermomonospora viridis.

    abstract::Maximum yields of amylase were produced by the thermophilic actinomycete Thermomonospora viridis in a modified Simpson and McCoy medium containing 1.5% corn starch and 0.5% mycological peptone with an initial pH 7.0. Best yields of amylase were obtained after incubation for 48 h, when the pH of the medium had risen to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.1.59-64.1977

    authors: Upton ME,Fogarty WM

    更新日期:1977-01-01 00:00:00

  • Proteomic approach for characterization of hop-inducible proteins in Lactobacillus brevis.

    abstract::Resistance to hops is a prerequisite for the capability of lactic acid bacteria to grow in beer and thus cause beer spoilage. Bactericidal hop compounds, mainly iso-alpha-acids, are described as ionophores which exchange H+ for cellular divalent cations, e.g., Mn2+, and thus dissipate ion gradients across the cytoplas...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00124-07

    authors: Behr J,Israel L,Gänzle MG,Vogel RF

    更新日期:2007-05-01 00:00:00

  • Tentative identification of methanogenic bacteria by fluorescence microscopy.

    abstract::Methanogenic bacteria, which are presently identified on the basis of cell morphology and substrate conversion to CH(4), can be differentiated from nonmethanogens and identified in pure or mixed culture on the basis of their autofluorescence under ultraviolet illumination. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.3.713-717.1977

    authors: Mink RW,Dugan PR

    更新日期:1977-03-01 00:00:00

  • Effect of storage time and temperature on the survival of Clostridium botulinum spores in acid media.

    abstract::Clostridium-botulinum type A and type B spores were stored in tomato juice (pH 4.2) and citric acid-phosphate buffer (pH 4.2) at 4, 22, and 32 degrees C for 180 days. The spore count was determined at different intervals over the 180-day storage period. There was no significant decrease in the number of type A spores ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.34.1.30-33.1977

    authors: Odlaug TE,Pflug IJ

    更新日期:1977-07-01 00:00:00

  • Differential proteomic analysis of Rickettsia prowazekii propagated in diverse host backgrounds.

    abstract::The obligate intracellular growth of Rickettsia prowazekii places severe restrictions on the analysis of rickettsial gene expression. With a small genome, predicted to code for 835 proteins, identifying which proteins are differentially expressed in rickettsiae that are isolated from different hosts or that vary in vi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.05140-11

    authors: Tucker AM,Driskell LO,Pannell LK,Wood DO

    更新日期:2011-07-01 00:00:00

  • Colonization and internalization of Salmonella enterica in tomato plants.

    abstract::The consumption of fresh tomatoes has been linked to numerous food-borne outbreaks involving various serovars of Salmonella enterica. Recent advances in our understanding of plant-microbe interactions have shown that human enteric pathogenic bacteria, including S. enterica, are adapted to survive in the plant environm...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03704-12

    authors: Zheng J,Allard S,Reynolds S,Millner P,Arce G,Blodgett RJ,Brown EW

    更新日期:2013-04-01 00:00:00

  • Chronic Airway Colonization by Achromobacter xylosoxidans in Cystic Fibrosis Patients Is Not Sustained by Their Domestic Environment.

    abstract::Achromobacter spp. are nonfermentative Gram-negative bacilli considered emergent pathogens in cystic fibrosis (CF). Although some cross-transmission events between CF patients have been described, Achromobacter strains were mostly patient specific, suggesting sporadic acquisitions from nonhuman reservoirs. However, so...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01739-18

    authors: Dupont C,Jumas-Bilak E,Doisy C,Aujoulat F,Chiron R,Marchandin H

    更新日期:2018-11-15 00:00:00

  • A Modular, Tn7-Based System for Making Bioluminescent or Fluorescent Salmonella and Escherichia coli Strains.

    abstract:UNLABELLED:Our goal was to develop a robust tagging method that can be used to track bacterial strains in vivo To address this challenge, we adapted two existing systems: a modular plasmid-based reporter system (pCS26) that has been used for high-throughput gene expression studies in Salmonella and Escherichia coli and...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01346-16

    authors: Shivak DJ,MacKenzie KD,Watson NL,Pasternak JA,Jones BD,Wang Y,DeVinney R,Wilson HL,Surette MG,White AP

    更新日期:2016-07-29 00:00:00