Tentative identification of methanogenic bacteria by fluorescence microscopy.

abstract：:In the current study, we adapted and optimized the lambda Red recombineering strategy to genetically manipulate the fastidious insect endosymbiont Sodalis glossinidius. This work greatly facilitates the application of genetics to the study of insect symbionts and should also prove useful in the context of long-awaited...

journal_title：Applied and environmental microbiology

authors： Pontes MH,Dale C

更新日期：2011-03-01 00:00:00

abstract：:The composition of the active microbial (bacterial and fungal) soil community in an arable wheat field subjected to different management practices was examined at five times during a 1-year period. Field sections were fertilized either at good agricultural practice (GAP) levels or at reduced levels (0.5x GAP) and were...

journal_title：Applied and environmental microbiology

authors： Girvan MS,Bullimore J,Ball AS,Pretty JN,Osborn AM

更新日期：2004-05-01 00:00:00

abstract：:Symbioses with gut microorganisms provides a means by which terrestrial herbivores are able to obtain energy. These microorganisms ferment cell wall materials of plants to short-chain fatty acids (SCFA), which are then absorbed and used by the host animal. Many marine herbivorous fishes contain SCFA (predominantly ace...

journal_title：Applied and environmental microbiology

authors： Mountfort DO,Campbell J,Clements KD

更新日期：2002-03-01 00:00:00

abstract：:Pep5 is a cationic pore-forming lantibiotic produced by Staphylococcus epidermidis strain 5. The producer strain protects itself from the lethal action of its own bacteriocin through the 69-amino-acid immunity peptide PepI. The N-terminal segment of PepI contains a 20-amino-acid stretch of apolar residues, whereas the...

journal_title：Applied and environmental microbiology

authors： Hoffmann A,Schneider T,Pag U,Sahl HG

更新日期：2004-06-01 00:00:00

abstract：:It has long been assumed that prolonged holding of environmental samples at the ambient air temperature prior to bacteriological analysis is detrimental to isolation and detection of Vibrio cholerae, the causative agent of pandemic cholera. The present study was aimed at understanding the effect of transporting enviro...

journal_title：Applied and environmental microbiology

authors： Alam M,Sadique A,Nur-A-Hasan,Bhuiyan NA,Nair GB,Siddique AK,Sack DA,Ahsan S,Huq A,Sack RB,Colwell RR

更新日期：2006-03-01 00:00:00

abstract：:Monitoring cell growth and measuring physical features of food-borne pathogenic bacteria are important for better understanding the conditions under which these organisms survive and proliferate. To address this challenge, buoyant masses of live and dead Escherichia coli O157:H7 and Listeria innocua were measured usin...

journal_title：Applied and environmental microbiology

authors： Lewis CL,Craig CC,Senecal AG

更新日期：2014-06-01 00:00:00

abstract：:Light microscopy and a radioassay detected no significant difference in adherence of Kanagawa-positive and Kanagawa-negative strains of Vibrio parahaemolyticus to human epithelial cell lines. ...

journal_title：Applied and environmental microbiology

authors： Gingras SP,Howard LV

更新日期：1980-02-01 00:00:00

abstract：:The adaptation of Lactobacillus sakei to a meat environment is reflected in its metabolic potential. For instance, the ability to utilize arginine through the arginine deiminase (ADI) pathway, resulting in additional ATP, represents a competitive benefit. In L. sakei CTC 494, the arc operon (arcABCTDR) shows the same ...

journal_title：Applied and environmental microbiology

authors： Rimaux T,Rivière A,Illeghems K,Weckx S,De Vuyst L,Leroy F

更新日期：2012-07-01 00:00:00

abstract：:The ionic and nonionic surfactants sodium dodecyl sulfate and Triton X-100, respectively, eluted two viruses, phi X174 and PRD1, which were adsorbed to the ionic and nonionic binding membranes cationic polysulfone and nitrocellulose, respectively. Results indicated that complete elution was readily achieved only when ...

journal_title：Applied and environmental microbiology

authors： Fujito BT,Lytle CD

更新日期：1996-09-01 00:00:00

abstract：:Four membrane filter methods for the enumeration of fecal coliforms were compared for accuracy, specificity, and recovery. Water samples were taken several times from 13 marine, 1 estuarine, and 4 freshwater sites around Puerto Rico, from pristine waters and waters receiving treated and untreated sewage and effluent f...

journal_title：Applied and environmental microbiology

authors： Santiago-Mercado J,Hazen TC

更新日期：1987-12-01 00:00:00

abstract：:A negative enrichment procedure was developed which was capable of isolating Legionella pneumophila directly from seeded air-conditioning cooling tower water onto laboratory media. This procedure was based on an 8-h incubation under conditions that were bactericidal to the indigenous water microflora but merely bacter...

journal_title：Applied and environmental microbiology

authors： Thorpe TC,Miller RD

更新日期：1980-10-01 00:00:00

abstract：:Fermentation of the pentose sugar xylose to ethanol in lignocellulosic biomass would make bioethanol production economically more competitive. Saccharomyces cerevisiae, an efficient ethanol producer, can utilize xylose only when expressing the heterologous genes XYL1 (xylose reductase) and XYL2 (xylitol dehydrogenase)...

journal_title：Applied and environmental microbiology

authors： Johansson B,Christensson C,Hobley T,Hahn-Hägerdal B

更新日期：2001-09-01 00:00:00

abstract：:Fresh produce is known to carry nonpathogenic epiphytic microorganisms. During agricultural production and harvesting, leafy greens can become contaminated with antibiotic-resistant pathogens or commensals from animal and human sources. As lettuce does not undergo any inactivation or preservation treatment during proc...

journal_title：Applied and environmental microbiology

authors： Holvoet K,Sampers I,Callens B,Dewulf J,Uyttendaele M

更新日期：2013-11-01 00:00:00

abstract：:The facultative anaerobe Thauera aromatica strain AR-1 uses 3,5-dihydroxybenzoate (3,5-DHB) as a sole carbon and energy source under anoxic conditions using an unusual oxidative strategy to overcome aromatic ring stability. A 25-kb gene cluster organized in four main operons encodes the anaerobic degradation pathway f...

journal_title：Applied and environmental microbiology

authors： Pacheco-Sánchez D,Molina-Fuentes Á,Marín P,Díaz-Romero A,Marqués S

更新日期：2019-01-09 00:00:00

abstract：:Legionella pneumophila proliferates in freshwater environments at temperatures ranging from 25 to 45°C. To investigate the preference of different sequence types (ST) for a specific temperature range, growth of L. pneumophila serogroup 1 (SG1) ST1 (environmental strains), ST47, and ST62 (disease-associated strains) wa...

journal_title：Applied and environmental microbiology

authors： van der Kooij D,Brouwer-Hanzens AJ,Veenendaal HR,Wullings BA

更新日期：2016-10-27 00:00:00

abstract：:The extensive use of antibiotics in hospitals and in the animal breeding industry has promoted antibiotic resistance in bacteria, which resulted in the emergence of a large number of antibiotic resistance genes in the intestinal tract of human and farmed animals. Genetic exchange of resistance genes between the two ec...

journal_title：Applied and environmental microbiology

authors： Wang C,Song Y,Tang N,Zhang G,Leclercq SO,Feng J

更新日期：2020-12-11 00:00:00

abstract：:Previously, we demonstrated conjugal transfer of a specially constructed shuttle vector, pRDB5, from the human colonic anaerobe Bacteroides uniformis to the ruminal anaerobe Prevotella (Bacteroides) ruminicola B(1)4. We have now shown that naturally occurring gene transfer elements in Bacteroides species and Prevotell...

journal_title：Applied and environmental microbiology

authors： Shoemaker NB,Wang GR,Salyers AA

更新日期：1992-04-01 00:00:00

abstract：:Freshwater lakes harbor complex microbial communities, but these ecosystems are often dominated by acI Actinobacteria Members of this cosmopolitan lineage are proposed to bolster heterotrophic growth using phototrophy because their genomes encode actino-opsins (actR). This model has been difficult to validate experime...

journal_title：Applied and environmental microbiology

authors： Dwulit-Smith JR,Hamilton JJ,Stevenson DM,He S,Oyserman BO,Moya-Flores F,Garcia SL,Amador-Noguez D,McMahon KD,Forest KT

更新日期：2018-11-30 00:00:00

abstract：:There is a growing interest in the use of microbial fermentation for the generation of high-demand, high-purity chemicals using cheap feedstocks in an environmentally friendly manner. One example explored here is the production of isoprene (C5H8), a hemiterpene, which is primarily polymerized to polyisoprene in synthe...

journal_title：Applied and environmental microbiology

authors： Diner BA,Fan J,Scotcher MC,Wells DH,Whited GM

更新日期：2017-12-15 00:00:00

abstract：:Nonribosomal peptides (NRPs) are a class of secondary metabolites usually produced by microorganisms. They are of paramount importance in different applications, including biocontrol and pharmacy. Brevibacillus spp. are a rich source of NRPs yet have received little attention. In this study, we characterize four novel...

journal_title：Applied and environmental microbiology

authors： Li Z,de Vries RH,Chakraborty P,Song C,Zhao X,Scheffers DJ,Roelfes G,Kuipers OP

更新日期：2020-11-24 00:00:00

abstract：:Pyocins are bacteriocins secreted by Pseudomonas aeruginosa, and they assist in the colonization of different niches. A major subset of these antibacterial proteins adopt a modular organization characteristic of polymorphic toxins. They include a receptor-binding domain, a segment enabling membrane passage, and a toxi...

journal_title：Applied and environmental microbiology

authors： Ghequire MGK,Öztürk B

更新日期：2018-08-31 00:00:00

abstract：:O-antigen biosynthetic (wbf) regions for Vibrio cholerae serogroups O5, O8, and O108 were isolated and sequenced. Sequences were compared to those of other published V. cholerae O-antigen regions. These wbf regions showed a high degree of heterogeneity both in gene content and in gene order. Genes identified frequentl...

journal_title：Applied and environmental microbiology

authors： Aydanian A,Tang L,Morris JG,Johnson JA,Stine OC

更新日期：2011-04-01 00:00:00

abstract：:Clostridium difficile causes a potentially fatal diarrheal disease through the production of its principal virulence factors, toxin A and toxin B. The tcdC gene is thought to encode a negative regulator of toxin production. Therefore, increased toxin production, and hence increased virulence, is often inferred in stra...

journal_title：Applied and environmental microbiology

authors： Cartman ST,Kelly ML,Heeg D,Heap JT,Minton NP

更新日期：2012-07-01 00:00:00

abstract：:To examine whether the heat-labile enterotoxin gene in porcine enterotoxigenic Escherichia coli (ETEC) strains is as divergent as in human ETEC strains, we sequenced the heat-labile and heat-stable toxin genes from 52 and 33 porcine ETEC strains, respectively. We found that the STa gene is identical, that the LT gene ...

journal_title：Applied and environmental microbiology

authors： Zhang C,Rausch D,Zhang W

更新日期：2009-10-01 00:00:00

abstract：:Histological observations were made on the penetration of hyphae of Rhizopus oligosporus into soybean cotyledons in tempeh, an Indonesian soybean food. Hyphal penetrations averaged one per 1,400 mum (+/-390 mum) on the curved (outer) cotyledon surface and one per 1,010 mum (+/-340 mum) on the flat (inner) one. Hyphae ...

journal_title：Applied and environmental microbiology

authors： Jurus AM,Sundberg WJ

更新日期：1976-08-01 00:00:00

abstract：:Strains of Xylella fastidiosa isolated from grape, almond, maple, and oleander were characterized by enterobacterial repetitive intergenic consensus sequence-, repetitive extragenic palindromic element (REP)-, and random amplified polymorphic DNA (RAPD)-PCR; contour-clamped homogeneous electric field (CHEF) gel electr...

journal_title：Applied and environmental microbiology

authors： Hendson M,Purcell AH,Chen D,Smart C,Guilhabert M,Kirkpatrick B

更新日期：2001-02-01 00:00:00

abstract：:The temperate bacteriophage BK5-T was isolated from Streptococcus cremoris BK5 by induction with mitomycin C. Electron microscopy revealed that BK5-T DNA consists of linear molecules, ranging in size from 39.7 to 46 kilobase pairs. Restriction analysis of self-ligated BK5-T DNA showed that the ends of the DNA were not...

journal_title：Applied and environmental microbiology

authors： Lakshmidevi G,Davidson BE,Hillier AJ

更新日期：1988-04-01 00:00:00

abstract：:The geochemical cycling of cobalt (Co) has often been considered to be controlled by the scavenging and oxidation of Co(II) on the surface of manganese [Mn(III,IV)] oxides or manganates. Because Mn(II) oxidation in the environment is often catalyzed by bacteria, we have investigated the ability of Mn(II)-oxidizing bac...

journal_title：Applied and environmental microbiology

authors： Lee Y,Tebo BM

更新日期：1994-08-01 00:00:00

abstract：:The thermophilic bacterium Bacillus thermoantarcticus produces two exocellular polysaccharides (EPS 1 and EPS 2), which can be obtained from the supernatant of liquid cultures by cold-ethanol precipitation, in yields as high as 400 mg liter(sup-1). The EPS fraction was produced with all substrates tested, although a h...

journal_title：Applied and environmental microbiology

authors： Manca MC,Lama L,Improta R,Esposito E,Gambacorta A,Nicolaus B

更新日期：1996-09-01 00:00:00

abstract：:Reductive dechlorination of the ortho moiety of polychlorinated biphenyls (PCBs) as well as of meta and para moieties is shown to occur in anaerobic enrichments of Baltimore Harbor sediments. These estuarine sediments ortho dechlorinated 2,3,5,6-chlorinated biphenyl (CB), 2,3,5-CB, and 2,3,6-CB in freshwater or estuar...

journal_title：Applied and environmental microbiology

authors： Berkaw M,Sowers KR,May HD

更新日期：1996-07-01 00:00:00