Abstract:
:Previously, we demonstrated conjugal transfer of a specially constructed shuttle vector, pRDB5, from the human colonic anaerobe Bacteroides uniformis to the ruminal anaerobe Prevotella (Bacteroides) ruminicola B(1)4. We have now shown that naturally occurring gene transfer elements in Bacteroides species and Prevotella ruminicola can also be transferred between these two genera. A self-transmissible chromosomal element originally found in a clinical isolate of Bacteroides fragilis (Tcr Emr 12256) was transferred from B. uniformis 0061 to P. ruminicola B(1)4 and from P. ruminicola B(1)4 back to B. uniformis or to another human colonic species, Bacteroides thetaiotaomicron. Similarly, a conjugative plasmid (pRRI4) originally found in P. ruminicola 223 was transferred from P. ruminicola B(1)4 to B. uniformis or B. thetaiotaomicron. pRRI4 could be transferred from the colonic Bacteroides species only if the donor strain contained the Tcr Emr 12256 element in its chromosome. These results show that transfer of naturally occurring elements can be demonstrated under laboratory conditions. Evidence that such transfers may actually have occurred in nature came from our finding that the tetracycline resistance (Tcr) gene on the P. ruminicola plasmid pRRI4 hybridized on high-stringency Southern blots with the Tcr gene found on the Bacteroides Tcr elements. The presence of the same gene in such distantly related genera of bacteria is most likely to have occurred as a result of horizontal transfer.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Shoemaker NB,Wang GR,Salyers AAdoi
10.1128/AEM.58.4.1313-1320.1992keywords:
subject
Has Abstractpub_date
1992-04-01 00:00:00pages
1313-20issue
4eissn
0099-2240issn
1098-5336journal_volume
58pub_type
杂志文章abstract::The psychrotolerant acidophile Acidithiobacillus ferrivorans has been identified from cold environments and has been shown to use ferrous iron and inorganic sulfur compounds as its energy sources. A bioinformatic evaluation presented in this study suggested that Acidithiobacillus ferrivorans utilized a ferrous iron ox...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02989-12
更新日期:2013-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.3.578-584.1982
更新日期:1982-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.2.1141-1147.2006
更新日期:2006-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.1.377-378.1995
更新日期:1995-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.12.4381-4387.1996
更新日期:1996-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01313-20
更新日期:2020-09-17 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.06524-11
更新日期:2011-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.1.61-66.1999
更新日期:1999-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.67.4.1522-1528.2001
更新日期:2001-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.2.430-434.1982
更新日期:1982-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.51.4.699-702.1986
更新日期:1986-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/aem.69.7.3826-3832.2003
更新日期:2003-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2017-05-31 00:00:00
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pub_type: 杂志文章
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更新日期:2016-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.7.2262-2264.1990
更新日期:1990-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.9.3394-3398.1997
更新日期:1997-09-01 00:00:00
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更新日期:2001-02-01 00:00:00
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更新日期:1997-01-01 00:00:00
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更新日期:2009-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.60.11.4039-4046.1994
更新日期:1994-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.33.3.577-584.1977
更新日期:1977-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.37.1.80-84.1979
更新日期:1979-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.2.394-402.1997
更新日期:1997-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.46.1.191-197.1983
更新日期:1983-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.9.3216-3220.1995
更新日期:1995-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.9.2280-2286.1989
更新日期:1989-09-01 00:00:00
abstract::Reductive dechlorination of the ortho moiety of polychlorinated biphenyls (PCBs) as well as of meta and para moieties is shown to occur in anaerobic enrichments of Baltimore Harbor sediments. These estuarine sediments ortho dechlorinated 2,3,5,6-chlorinated biphenyl (CB), 2,3,5-CB, and 2,3,6-CB in freshwater or estuar...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.7.2534-2539.1996
更新日期:1996-07-01 00:00:00