Molecular characterization of races and vegetative compatibility groups in Fusarium oxysporum f. sp. vasinfectum.

abstract：:A metagenomic library of a petroleum-contaminated soil was constructed in a fosmid vector that allowed heterologous expression of metagenomic DNA. The library, consisting of 6.5 Gb of metagenomic DNA, was screened for extradiol dioxygenase (Edo) activity using catechol and 2,3-dihydroxybiphenyl as the substrates. Fift...

journal_title：Applied and environmental microbiology

authors： Terrón-González L,Martín-Cabello G,Ferrer M,Santero E

更新日期：2016-04-04 00:00:00

abstract：:Escherichia coli isolates from rectal swabs from 62 chickens and stools from 42 children living in close contact with chickens on the same farms in Kiambu district, Kenya, were compared for their genetic relatedness. Antibiotic susceptibility profiles broadly categorized isolates from the children and from the chicken...

journal_title：Applied and environmental microbiology

authors： Kariuki S,Gilks C,Kimari J,Obanda A,Muyodi J,Waiyaki P,Hart CA

更新日期：1999-02-01 00:00:00

abstract：:The diversity of microcystin-producing cyanobacteria in the western basin of Lake Erie was studied using sequence analysis of mcyA gene fragments. Distinct populations of potentially toxic Microcystis and Planktothrix were found in spatially isolated locations. This study highlights previously undocumented diversity o...

journal_title：Applied and environmental microbiology

authors： Rinta-Kanto JM,Wilhelm SW

更新日期：2006-07-01 00:00:00

abstract：:Thirty presumptive Aeromonas hydrophila isolates were collected from estuarine sources using Rimler-Shotts media. Many of the isolates, especially the strains from a low-salinity site, were also identified as A. hydrophila using the API 20E system and two differential techniques devised for the identification of A. hy...

journal_title：Applied and environmental microbiology

authors： Davis JW,Sizemore RK

更新日期：1981-09-01 00:00:00

abstract：:The microorganisms inhabiting a 91 degrees C hot spring in Yellowstone National Park were characterized by sequencing 5S rRNAs isolated from the mixed, natural microflora without cultivation. By comparisons of these sequences with reference sequences, the phylogenetic relationships of the hot spring organisms to bette...

journal_title：Applied and environmental microbiology

authors： Stahl DA,Lane DJ,Olsen GJ,Pace NR

更新日期：1985-06-01 00:00:00

abstract：:Chlamydiae are important pathogens of humans and animals but diagnosis of chlamydial infections is still hampered by inadequate detection methods. Fluorescence in situ hybridization (FISH) using rRNA-targeted oligonucleotide probes is widely used for the investigation of uncultured bacteria in complex microbial commun...

journal_title：Applied and environmental microbiology

authors： Poppert S,Essig A,Marre R,Wagner M,Horn M

更新日期：2002-08-01 00:00:00

abstract：:During cold storage after milk collection, psychrotrophic bacterial populations dominate the microflora, and their extracellular enzymes, mainly proteases and lipases, contribute to the spoilage of dairy products. The diversity, dynamics, and enzymatic traits of culturable psychrotrophs in raw milk from four farms wer...

journal_title：Applied and environmental microbiology

authors： Hantsis-Zacharov E,Halpern M

更新日期：2007-11-01 00:00:00

abstract：:Bacterial source tracking is used to apportion fecal pollution among putative sources. Within this context, library-independent markers are genetic or phenotypic traits that can be used to identify the host origin without a need for library-dependent classification functions. The objective of this project was to use m...

journal_title：Applied and environmental microbiology

authors： Soule M,Kuhn E,Loge F,Gay J,Call DR

更新日期：2006-03-01 00:00:00

abstract：:Poultry are considered a major reservoir and source of human campylobacteriosis, but the roles of environmental reservoirs, including wild birds, have not been assessed in depth. In this study, we isolated and characterized Campylobacter jejuni from western jackdaws (n = 91, 43%), mallard ducks (n = 82, 76%), and phea...

journal_title：Applied and environmental microbiology

authors： Kovanen S,Rossi M,Pohja-Mykrä M,Nieminen T,Raunio-Saarnisto M,Sauvala M,Fredriksson-Ahomaa M,Hänninen ML,Kivistö R

更新日期：2019-02-06 00:00:00

abstract：:The growing mycelia of Trichoderma reesei Rut-C30 are richly endowed with endoplasmic reticula and a variety of pleomorphic subcellular bodies. Mycelia of the culture growing in presence of avicel pH101 was fractionated in sucrose density gradients, and several morphologically and biochemically distinct fractions were...

journal_title：Applied and environmental microbiology

authors： Glenn M,Ghosh A,Ghosh BK

更新日期：1985-11-01 00:00:00

abstract：:A beta-1,3-galactosyl-N-acetylhexosamine phosphorylase (GalGlyNAcP) homolog gene was cloned from Vibrio vulnificus CMCP6. In synthetic reactions, the recombinant enzyme acted only with GlcNAc and GalNAc as acceptors in the presence of alpha-d-galactose-1-phosphate as a donor to form lacto-N-biose I (LNB) (Galbeta1 -->...

journal_title：Applied and environmental microbiology

authors： Nakajima M,Kitaoka M

更新日期：2008-10-01 00:00:00

abstract：:Three crude oil bioremediation techniques were applied in a randomized block field experiment simulating a coastal oil spill. Four treatments (no oil control, oil alone, oil plus nutrients, and oil plus nutrients plus an indigenous inoculum) were applied. In situ microbial community structures were monitored by phosph...

journal_title：Applied and environmental microbiology

authors： MacNaughton SJ,Stephen JR,Venosa AD,Davis GA,Chang YJ,White DC

更新日期：1999-08-01 00:00:00

abstract：:Treatment of secondary effluents with hydrogen peroxide (10 mg/liter)-ascorbic acid (10 mg/liter)-Cu2+ (0.5 mg/liter) for 60 min resulted in around 99% reduction of the initial plate count. Hydrogen peroxide could be replaced by other peroxygen compounds; ascorbic acid could be replaced by other reducing agents, of wh...

journal_title：Applied and environmental microbiology

authors： Ragab-Depre NJ

更新日期：1982-09-01 00:00:00

abstract：:There is a wealth of evidence indicating that mobile genetic elements can spread in natural microbial communities. However, little is known regarding the fraction of the community that actually engages in this behavior. Here we report on a new approach to quantify the fraction of a bacterial community that is able to ...

journal_title：Applied and environmental microbiology

authors： Musovic S,Dechesne A,Sørensen J,Smets BF

更新日期：2010-07-01 00:00:00

abstract：:We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of the rapeseed BnACCg8 gene flanked by the hly-specific target seq...

journal_title：Applied and environmental microbiology

authors： Rodríguez-Lázaro D,Pla M,Scortti M,Monzó HJ,Vázquez-Boland JA

更新日期：2005-12-01 00:00:00

abstract：:DNA probes that are specific for each of five predominant species of human colonic Bacteroides (B. thetaiotaomicron, B. uniformis, B. distasonis, "Bacteroides group 3452-A", and B. ovatus) were used to detect and enumerate these species in fecal samples from two adult volunteers. These five species are capable of ferm...

journal_title：Applied and environmental microbiology

authors： Kuritza AP,Shaughnessy P,Salyers AA

更新日期：1986-02-01 00:00:00

abstract：:The discovery of a letter intentionally filled with dried Bacillus anthracis spores in the office of a United States senator prompted the collection and quarantine of all mail in congressional buildings. This mail was subsequently searched for additional intentionally contaminated letters. A microbiological sampling s...

journal_title：Applied and environmental microbiology

authors： Beecher DJ

更新日期：2006-08-01 00:00:00

abstract：:Methylosinus trichosporium OB3b produces an extracellular copper-binding ligand (CBL) with high affinity for copper. Wild-type cells and mutants that express soluble methane monooxygenase (sMMO) in the presence and absence of copper (sMMOc) were used to obtain cell exudates that were separated and analyzed by size exc...

journal_title：Applied and environmental microbiology

authors： Téllez CM,Gaus KP,Graham DW,Arnold RG,Guzman RZ

更新日期：1998-03-01 00:00:00

abstract：:The relationship between physiochemical surface parameters and adhesion of bacterial cells to negatively charged polystyrene was studied. Cell surface hydrophobicity and electrokinetic potential were determined by contact angle measurement and electrophoresis, respectively. Both parameters influence cell adhesion. The...

journal_title：Applied and environmental microbiology

authors： van Loosdrecht MC,Lyklema J,Norde W,Schraa G,Zehnder AJ

更新日期：1987-08-01 00:00:00

abstract：:The internal pH of Clostridium acetobutylicum was determined at various stages during the growth of the organism. Even in the presence of significant quantities of acetic, butyric, and lactic acids, an internal pH of 6.2 was maintained. Experiments using N,N'-dicyclohexylcarbodiimide indicated that a functioning H+-AT...

journal_title：Applied and environmental microbiology

authors： Bowles LK,Ellefson WL

更新日期：1985-11-01 00:00:00

abstract：:Synthesis of the ligninolytic system of the wood-degrading fungus Phanerochaete chrysosporium is induced during secondary metabolism, brought about by nitrogen, carbon, or sulfur starvation. We describe here a strategy for selection of mutants which are ligninolytic (lignin----CO2) and overproduce lignin-degrading enz...

journal_title：Applied and environmental microbiology

authors： Tien M,Myer SB

更新日期：1990-08-01 00:00:00

abstract：:Thermostable and thermoactive beta-mannanase (1,4-beta-D-mannan mannanohydrolase [EC 3.2.1.78]), beta-mannosidase (beta-D-mannopyranoside hydrolase [EC 3.2.1.25]) and alpha-galactosidase (alpha-D-galactoside galactohydrolase [EC 3.2.1.22]) were purified to homogeneity from cell extracts and extracellular culture super...

journal_title：Applied and environmental microbiology

authors： Duffaud GD,McCutchen CM,Leduc P,Parker KN,Kelly RM

更新日期：1997-01-01 00:00:00

abstract：:Pullulanase (EC 3.2.1.41), an important enzyme in the production of starch syrup, catalyzes the hydrolysis of α-1,6 glycosidic bonds in complex carbohydrates. A double mutant (DM; D437H/D503Y) form of Bacillus deramificans pullulanase was recently constructed to enhance the thermostability and catalytic efficiency of ...

journal_title：Applied and environmental microbiology

authors： Duan X,Wu J

更新日期：2015-03-01 00:00:00

abstract：:Specific Saccharomyces cerevisiae strains were recently found to be capable of efficiently utilizing inulin, but genetic mechanisms of inulin hydrolysis in yeast remain unknown. Here we report functional characteristics of invertase SUC2 from strain JZ1C and demonstrate that SUC2 is the key enzyme responsible for inul...

journal_title：Applied and environmental microbiology

authors： Wang SA,Li FL

更新日期：2013-01-01 00:00:00

abstract：:There is much interest in the use of seed-applied bacteria for biocontrol and biofertilization, and several commercial products are available. However, many attempts to use this strategy fail because the seed-applied bacteria do not colonize the rhizosphere. Mechanisms of rhizosphere colonization may involve active ba...

journal_title：Applied and environmental microbiology

authors： Knox OG,Killham K,Artz RR,Mullins C,Wilson M

更新日期：2004-08-01 00:00:00

abstract：:This study aimed to elucidate determinants of heat resistance in Escherichia coli by comparing the composition of membrane lipids, as well as gene expression, in heat-resistant E. coli AW1.7 and heat-sensitive E. coli GGG10 with or without heat shock. The survival of E. coli AW1.7 at late exponential phase was 100-fol...

journal_title：Applied and environmental microbiology

authors： Ruan L,Pleitner A,Gänzle MG,McMullen LM

更新日期：2011-05-01 00:00:00

abstract：:Many food-grade bacteria produce exopolysaccharides (EPS) that affect the texture of fermented food products and that may be involved in probiotic properties. Propionibacterium freudenreichii is a Gram-positive food-grade bacterium with reported probiotic capabilities that is widely used as starter in Swiss-type chees...

journal_title：Applied and environmental microbiology

authors： Deutsch SM,Le Bivic P,Hervé C,Madec MN,LaPointe G,Jan G,Le Loir Y,Falentin H

更新日期：2010-05-01 00:00:00

abstract：:We analyzed the communities of soil basidiomycetes in agroecosystems that differ in tillage history at the Kellogg Biological Station Long-Term Ecological Research site near Battle Creek, Michigan. The approach combined soil DNA extraction through a bead-beating method modified to increase recovery of fungal DNA, PCR ...

journal_title：Applied and environmental microbiology

authors： Lynch MD,Thorn RG

更新日期：2006-11-01 00:00:00

abstract：:Biobleaching of hardwood unbleached kraft pulp (UKP) by Phanerochaete chrysosporium and Trametes versicolor was studied in the solid-state fermentation system with different culture media. In this fermentation system with low-nitrogen and high-carbon culture medium, pulp brightness increased by 15 and 30 points after ...

journal_title：Applied and environmental microbiology

authors： Katagiri N,Tsutsumi Y,Nishida T

更新日期：1995-02-01 00:00:00

abstract：:Environmental bacteria are exposed to a myriad of biotic interactions that influence their function and survival. The grazing activity of protozoan predators significantly impacts the dynamics, diversification, and evolution of bacterial communities in soil ecosystems. To evade protozoan predation, bacteria employ var...

journal_title：Applied and environmental microbiology

authors： Mazzola M,de Bruijn I,Cohen MF,Raaijmakers JM

更新日期：2009-11-01 00:00:00