Selection and characterization of mutants of Phanerochaete chrysosporium exhibiting ligninolytic activity under nutrient-rich conditions.

abstract：:An enrichment method using d-cycloserine was designed for the isolation of spontaneous mutants of Zymomonas mobilis deficient in glucose or fructose utilization. The mutants could easily be isolated since they represented 80 to 90% of the population after two and three enrichment cycles. Glucokinase and fructokinase a...

journal_title：Applied and environmental microbiology

authors： Ait-Abdelkader N,Pencreach G,Joset F,Baratti JC

更新日期：1996-03-01 00:00:00

abstract：:The putative role of biting flies in Bartonella transmission among ruminants was investigated. Amplification of the Bartonella citrate synthase gene from 83 Hippoboscidae was detected in 94% of 48 adult Lipoptena cervi flies, 71% of 17 adult Hippobosca equina flies, 100% of 20 adult Melophagus ovinus flies, and 100% o...

journal_title：Applied and environmental microbiology

authors： Halos L,Jamal T,Maillard R,Girard B,Guillot J,Chomel B,Vayssier-Taussat M,Boulouis HJ

更新日期：2004-10-01 00:00:00

abstract：:Lecithin agar was developed on which phospholipase C produced turbid zones and phospholipase A produced clear zones. Reactions on lecithin agar agreed 74% of the time with reactions in egg yolk broth. On lecithin agar, interpretation was easier, phospholipase A was detectable, and opaque zones were visible 1 or 2 days...

journal_title：Applied and environmental microbiology

authors： Chrisope GL,Fox CW,Marshall RT

更新日期：1976-05-01 00:00:00

abstract：:Few techniques are currently available for quantifying specific prokaryotic taxa in environmental samples. Quantification of specific genotypes has relied mainly on oligonucleotide hybridization to extracted rRNA or intact rRNA in whole cells. However, low abundance and cellular rRNA content limit the application of t...

journal_title：Applied and environmental microbiology

authors： Suzuki MT,Taylor LT,DeLong EF

更新日期：2000-11-01 00:00:00

abstract：:A highly specific medium was developed for the enumeration of lactate-utilizing bacteria in the rumen of sheep. This medium, which contained 2.0% lactate, 2.0% Trypticase, 0.2% yeast extract, and volatile fatty acids, hemin, and trace elements in place of rumen fluid, enabled high counts (42 x 10 to 190 x 10/g of inge...

journal_title：Applied and environmental microbiology

authors： Mackie RI,Heath S

更新日期：1979-09-01 00:00:00

abstract：:The milk supply is considered a primary route for a bioterrorism attack with Bacillus anthracis spores because typical high-temperature short-time (HTST) pasteurization conditions cannot inactivate spores. In the event of intentional contamination, an effective method to inactivate the spores in milk under HTST proces...

journal_title：Applied and environmental microbiology

authors： Xu S,Labuza TP,Diez-Gonzalez F

更新日期：2008-06-01 00:00:00

abstract：:Mexico is located in a transition zone between the Nearctic and Neotropical biogeographical regions and contains a rich and unique biodiversity. A total of 496 Bacillus thuringiensis strains were isolated from 503 soil samples collected from the five macroregions of the country. The characterization of the strain coll...

journal_title：Applied and environmental microbiology

authors： Bravo A,Sarabia S,Lopez L,Ontiveros H,Abarca C,Ortiz A,Ortiz M,Lina L,Villalobos FJ,Peña G,Nuñez-Valdez ME,Soberón M,Quintero R

更新日期：1998-12-01 00:00:00

abstract：:Escherichia coli offers unparalleled engineering capacity in the context of heterologous natural product biosynthesis. However, as with other heterologous hosts, cellular metabolism must be designed or redesigned to support final compound formation. This task is at once complicated and aided by the fact that the cell ...

journal_title：Applied and environmental microbiology

authors： Jiang M,Stephanopoulos G,Pfeifer BA

更新日期：2012-04-01 00:00:00

abstract：:Of some 350 microorganisms screened, four strains of Pithomyces species were found to carry out regio-selective hydroxylation of patchoulol, a sesquiterpene, to 10-hydroxypatchoulol: Pithomyces sp. NRJ201, P. chartarum NRJ210, and, to a lesser extent, P. cynodontis ATCC 26150 and P. atro-olivaceus IFO 6651 were found ...

journal_title：Applied and environmental microbiology

authors： Suhara Y,Itoh S,Ogawa M,Yokose K,Sawada T,Sano T,Ninomiya R,Maruyama HB

更新日期：1981-08-01 00:00:00

abstract：:There is a wealth of evidence indicating that mobile genetic elements can spread in natural microbial communities. However, little is known regarding the fraction of the community that actually engages in this behavior. Here we report on a new approach to quantify the fraction of a bacterial community that is able to ...

journal_title：Applied and environmental microbiology

authors： Musovic S,Dechesne A,Sørensen J,Smets BF

更新日期：2010-07-01 00:00:00

abstract：:Fusarium isolates from specific diseased sorghum plants and rain-soaked wheat and wheat flour associated with human mycotoxicosis in India have been screened for their toxigenic potential. Of the 322 isolates screened, 11 isolates were found to produce deoxynivalenol in concentrations ranging from 0.01 to 186 microgra...

journal_title：Applied and environmental microbiology

authors： Ramakrishna Y,Bhat RV,Ravindranath V

更新日期：1989-10-01 00:00:00

abstract：:The Association of Official Analytical Chemists (AOAC) test for assessing the tuberculocidal activity of disinfectants has been shown to be variable. A modified AOAC test, which substituted Middlebrook 7H9 broth as the primary subculture medium and used neutralization by dilution, was compared with the standard AOAC m...

journal_title：Applied and environmental microbiology

authors： Cole EC,Rutala WA,Nessen L,Wannamaker NS,Weber DJ

更新日期：1990-06-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH) and rRNA slot blot hybridization with 16S rRNA-targeted oligonucleotide probes were used to investigate the phylogenetic composition of a marine Arctic sediment (Svalbard). FISH resulted in the detection of a large fraction of microbes living in the top 5 cm of the sediment. U...

journal_title：Applied and environmental microbiology

authors： Ravenschlag K,Sahm K,Amann R

更新日期：2001-01-01 00:00:00

abstract：:Saccharomyces cerevisiae normally cannot assimilate mannitol, a promising brown macroalgal carbon source for bioethanol production. The molecular basis of this inability remains unknown. We found that cells capable of assimilating mannitol arose spontaneously from wild-type S. cerevisiae during prolonged culture in ma...

journal_title：Applied and environmental microbiology

authors： Chujo M,Yoshida S,Ota A,Murata K,Kawai S

更新日期：2015-01-01 00:00:00

abstract：:Enterobacteriaceae-associated blaCTX-M genes have become globally widespread within the past 30 years. Among isolates from Washington State cattle, Escherichia coli strains carrying blaCTX-M (CTX-M E. coli strains) were absent from a set of 2008 isolates but present in a set of isolates from 2011. On 30 Washington Sta...

journal_title：Applied and environmental microbiology

authors： Davis MA,Sischo WM,Jones LP,Moore DA,Ahmed S,Short DM,Besser TE

更新日期：2015-07-01 00:00:00

abstract：:The function of small secreted proteins (SSPs) in saprotrophic fungi is, for the most part, unknown. The white-rot mushroom Pleurotus ostreatus produces considerable amounts of SSPs at the onset of secondary metabolism, during colony development, and in response to chemical compounds such as 5-hydroxymethylfurfural an...

journal_title：Applied and environmental microbiology

authors： Feldman D,Amedi N,Carmeli S,Yarden O,Hadar Y

更新日期：2019-07-18 00:00:00

abstract：:The rates of mineralization of phenol, benzoate, benzylamine, p-nitrophenol, and di(2-ethylhexyl) phthalate added to lake water at concentrations ranging from a few picograms to nanograms per milliliter were directly proportional to chemical concentration. The rates were still linear at levels of <1 pg of phenol or p-...

journal_title：Applied and environmental microbiology

authors： Rubin HE,Subba-Rao RV,Alexander M

更新日期：1982-05-01 00:00:00

abstract：:Sporulation of Bacillus larvae NRRL B-3650 occurred only at aeration rates lower than those used for cultivation of most Bacillus species. One possible explanation for the requirement for a low level of aeration in B. larvae is that toxic forms of oxygen such as H(2)O(2) and superoxide are involved. The superoxide dis...

journal_title：Applied and environmental microbiology

authors： Dingman DW,Stahly DP

更新日期：1984-06-01 00:00:00

abstract：:A high biomasses of Cladophora, a filamentous green alga, is found mainly during the summer along the shores of Lake Michigan. In this study, the abundance and persistence of the fecal indicator bacterium Escherichia coli and sulfate-reducing bacteria (SRB) on Cladophora mats collected at Lake Michigan beaches were ev...

journal_title：Applied and environmental microbiology

authors： Olapade OA,Depas MM,Jensen ET,McLellan SL

更新日期：2006-03-01 00:00:00

abstract：:Allozyme electrophoresis and restriction fragment length polymorphism (RFLP) analyses were used to examine the genetic diversity of a collection of 18 Rhizobium leguminosarum bv. trifolii, 1 R. leguminosarum bv. viciae, and 2 R. meliloti strains. Allozyme analysis at 28 loci revealed 16 electrophoretic types. The mean...

journal_title：Applied and environmental microbiology

authors： Demezas DH,Reardon TB,Watson JM,Gibson AH

更新日期：1991-12-01 00:00:00

abstract：:Low concentrations of furfural are formed as a side product during the dilute acid hydrolysis of hemicellulose. Growth is inhibited by exposure to furfural but resumes after the complete reduction of furfural to the less toxic furfuryl alcohol. Growth-based selection was used to isolate a furfural-resistant mutant of ...

journal_title：Applied and environmental microbiology

authors： Miller EN,Jarboe LR,Yomano LP,York SW,Shanmugam KT,Ingram LO

更新日期：2009-07-01 00:00:00

abstract：:The primary goal of this study was to determine the conditions required for the effective inactivation of Bacillus anthracis spores on materials by using methyl bromide (MeBr) gas. Another objective was to obtain comparative decontamination efficacy data with three avirulent microorganisms to assess their potential fo...

journal_title：Applied and environmental microbiology

authors： Wood JP,Wendling M,Richter W,Lastivka A,Mickelsen L

更新日期：2016-01-22 00:00:00

abstract：:We designed five Pseudomonas-selective soil extract NAA media containing the selective properties of trimethoprim and sodium lauroyl sarcosine and 0 to 100% of the amount of Casamino Acids used in the classical Pseudomonas-selective Gould's S1 medium. All of the isolates were confirmed to be Pseudomonas by a Pseudomon...

journal_title：Applied and environmental microbiology

authors： Aagot N,Nybroe O,Nielsen P,Johnsen K

更新日期：2001-11-01 00:00:00

abstract：:Twenty-eight types of lysozyme-sensitive spores among seven Bacillus species representative of thermophiles, mesophiles, and psychrophiles were obtained spanning a 3,000-fold range in moist-heat resistance. The resistance within species was altered by demineralization of the native spores to protonated spores and remi...

journal_title：Applied and environmental microbiology

authors： Beaman TC,Gerhardt P

更新日期：1986-12-01 00:00:00

abstract：:An anaerobic diplococcoid bacterium able to degrade hydrolyzable tannins was isolated from the ruminal fluid of a goat fed desmodium (Desmodium ovalifolium), a tropical legume which contains levels as high as 17% condensed tannins. This strain grew under anaerobic conditions in the presence of up to 30 g of tannic aci...

journal_title：Applied and environmental microbiology

authors： Nelson KE,Pell AN,Schofield P,Zinder S

更新日期：1995-09-01 00:00:00

abstract：:Pseudomonas fluorescens 5R contains an NAH7-like plasmid (pKA1), and P. fluorescens 5R mutant 5RL contains a bioluminescent reporter plasmid (pUTK21) which was constructed by transposon mutagenesis. Polymerase chain reaction mapping confirmed the localization of lux transposon Tn4431 300 bp downstream from the start o...

journal_title：Applied and environmental microbiology

authors： Menn FM,Applegate BM,Sayler GS

更新日期：1993-06-01 00:00:00

abstract：:Myoviruses and podoviruses that infect cyanobacteria are the two major groups of marine cyanophages, but little is known of how their phylogenetic lineages are distributed in different habitats. In this study, we analyzed the phylogenetic relationships of cyanopodoviruses and cyanomyoviruses based on the existing geno...

journal_title：Applied and environmental microbiology

authors： Huang S,Zhang S,Jiao N,Chen F

更新日期：2015-01-01 00:00:00

abstract：:Botulinum neurotoxin (BoNT) serotype B (BoNT/B) is one of the serotypes of BoNT that causes deadly human botulism, though it is used clinically for treatment of many neuromuscular diseases. BoNT/B is produced by Clostridium botulinum, and it is secreted along with a group of neurotoxin-associated proteins (NAPs) in th...

journal_title：Applied and environmental microbiology

authors： Wang HH,Riding S,Lindo P,Singh BR

更新日期：2010-10-01 00:00:00

abstract：:Recently, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) have been increasingly isolated from veterinarians and companion animals. With a view to preventing the spread of MRSA and MRSP, we evaluated the occurrence and molecular characteristics of eac...

journal_title：Applied and environmental microbiology

authors： Ishihara K,Shimokubo N,Sakagami A,Ueno H,Muramatsu Y,Kadosawa T,Yanagisawa C,Hanaki H,Nakajima C,Suzuki Y,Tamura Y

更新日期：2010-08-01 00:00:00

abstract：:The scarcity of fresh water in the Mediterranean region necessitates the search for halotolerant agents of biological control of plant diseases that can be applied in arid-zone agriculture irrigated with saline water. Among 29 Trichoderma strains previously isolated from Mediterranean Psammocinia sp. sponges, the grea...

journal_title：Applied and environmental microbiology

authors： Gal-Hemed I,Atanasova L,Komon-Zelazowska M,Druzhinina IS,Viterbo A,Yarden O

更新日期：2011-08-01 00:00:00