Two-step freezing procedure for cryopreservation of rumen ciliates, an effective tool for creation of a frozen rumen protozoa bank.

abstract：:Two genes involved in aflatoxin B1 (AFB1) biosynthesis in Aspergillus parasiticus, nor-1 and ver-1, were localized to a 35-kb region on one A. parasiticus chromosome and to the genomic DNA fragment carried on a single cosmid, NorA. A physical and transcriptional map of the 35-kb genomic DNA insert in cosmid NorA was p...

journal_title：Applied and environmental microbiology

authors： Trail F,Mahanti N,Rarick M,Mehigh R,Liang SH,Zhou R,Linz JE

更新日期：1995-07-01 00:00:00

abstract：:Feeding of bovine milk to mice induced a high incidence of bacterial translocation from the intestines to the mesenteric lymph nodes, and the bacteria involved were mainly members of the family Enterobacteriaceae. Supplementation of the milk diet with bovine lactoferrin or a pepsin-generated hydrolysate of bovine lact...

journal_title：Applied and environmental microbiology

authors： Teraguchi S,Shin K,Ogata T,Kingaku M,Kaino A,Miyauchi H,Fukuwatari Y,Shimamura S

更新日期：1995-11-01 00:00:00

abstract：:The susceptibility of the Iberian ibex (Capra pyrenaica) to Mycoplasma conjunctivae ocular infection and the changes in their interaction over time were studied in terms of clinical outcome, molecular detection, and IgG immune response in a captive population that underwent a severe infectious keratoconjunctivitis (IK...

journal_title：Applied and environmental microbiology

authors： Fernández-Aguilar X,Cabezón O,Granados JE,Frey J,Serrano E,Velarde R,Cano-Manuel FJ,Mentaberre G,Ráez-Bravo A,Fandos P,López-Olvera JR

更新日期：2017-07-17 00:00:00

abstract：:Six laboratories actively involved in water virology research participated in a methods evaluation study, conducted under the auspices of the American Society for Testing and Materials Committee on Viruses in the Aquatic Environment, Task Force on Drinking Water. Each participant was asked to examine the Viradel (viru...

journal_title：Applied and environmental microbiology

authors： Melnick JL,Safferman R,Rao VC,Goyal S,Berg G,Dahling DR,Wright BA,Akin E,Stetler R,Sorber C

更新日期：1984-01-01 00:00:00

abstract：:Twenty-six lactic acid bacterium strains isolated from European dairy products were identified as Streptococcus thermophilus and characterized by bacterial growth and exopolysaccharide (EPS)-producing capacity in milk and enriched milk medium. In addition, the acidification rates of the different strains were compared...

journal_title：Applied and environmental microbiology

authors： Vaningelgem F,Zamfir M,Mozzi F,Adriany T,Vancanneyt M,Swings J,De Vuyst L

更新日期：2004-02-01 00:00:00

abstract：:Seabird ticks are known reservoirs of bacterial pathogens of medical importance; however, ticks parasitizing tropical seabirds have received less attention than their counterparts from temperate and subpolar regions. Recently, Rickettsia africae was described to infect seabird ticks of the western Indian Ocean and New...

journal_title：Applied and environmental microbiology

authors： Wilkinson DA,Dietrich M,Lebarbenchon C,Jaeger A,Le Rouzic C,Bastien M,Lagadec E,McCoy KD,Pascalis H,Le Corre M,Dellagi K,Tortosa P

更新日期：2014-06-01 00:00:00

abstract：:The diversity of rhizobia that form symbioses with roots of black locust (Robinia pseudoacacia L.), an economically important leguminous tree species, was examined by inoculating seedling root zones with samples of soil collected from the United States, Canada, and China. Bacteria were isolated from nodules, subcultur...

journal_title：Applied and environmental microbiology

authors： McCray Batzli J,Graves WR,van Berkum P

更新日期：1992-07-01 00:00:00

abstract：:Laboratory column studies were conducted at the Utah Water Research Laboratory, Logan, Utah, to evaluate reovirus removal from drinking water supplies by slow-rate sand filtration (SSF). Columns, constructed to simulate a full-scale SSF field operation, were inoculated with reovirus at ca. 1,000-times-greater concentr...

journal_title：Applied and environmental microbiology

authors： McConnell LK,Sims RC,Barnett BB

更新日期：1984-10-01 00:00:00

abstract：:Two constructs derived from the alpha-amylase gene (amyA) of Lactobacillus amylovorus were expressed in Lactobacillus plantarum, and their expression products were purified, characterized, and compared. These products correspond to the complete (AmyA) and truncated (AmyADelta) forms of alpha-amylase; AmyADelta lacks t...

journal_title：Applied and environmental microbiology

authors： Rodriguez Sanoja R,Morlon-Guyot J,Jore J,Pintado J,Juge N,Guyot JP

更新日期：2000-08-01 00:00:00

abstract：:A bioassay method was developed to measure the average intensity within a UV disinfection reactor. The survival of spores of Bacillus subtilis was determined as a function of UV dose to prepare a standard curve. Spores were added to unknown systems, and the survival rate was used to determine the average intensity. A ...

journal_title：Applied and environmental microbiology

authors： Qualls RG,Johnson JD

更新日期：1983-03-01 00:00:00

abstract：:Identification and classification of Vibrio species have relied upon band pattern methods (e.g., amplified fragment length polymorphism) and DNA-DNA hybridization. However, data generated by these methods cannot be used to build an online electronic taxonomy. In order to overcome these limitations, we developed the fi...

journal_title：Applied and environmental microbiology

authors： Thompson FL,Gomez-Gil B,Vasconcelos AT,Sawabe T

更新日期：2007-07-01 00:00:00

abstract：:Hyphomicrobium chloromethanicum CM2(T), an aerobic methylotrophic member of the alpha subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. H. chloromethanicum possesses an inducible enzyme system for utilization of chloromethane, in which two polypeptides (67-kDa CmuA...

journal_title：Applied and environmental microbiology

authors： McAnulla C,Woodall CA,McDonald IR,Studer A,Vuilleumier S,Leisinger T,Murrell JC

更新日期：2001-01-01 00:00:00

abstract：:Enterohemorrhagic Escherichia coli (EHEC) is a significant zoonotic pathogen causing severe disease associated with watery and bloody diarrhea, hemorrhagic colitis, and the hemolytic-uremic syndrome (HUS) in humans. Infections are frequently associated with contact with EHEC-contaminated ruminant feces. Both natural a...

journal_title：Applied and environmental microbiology

authors： Joris MA,Vanrompay D,Verstraete K,De Reu K,De Zutter L,Cox E

更新日期：2013-06-01 00:00:00

abstract：:Studies of small noncoding RNAs (sRNAs) have been conducted predominantly using culturable organisms, and the acquisition of further information about sRNAs from global environments containing uncultured organisms now is very important. In this study, hot spring water (57°C, pH 8.1) was collected directly from the und...

journal_title：Applied and environmental microbiology

authors： Murakami S,Fujishima K,Tomita M,Kanai A

更新日期：2012-02-01 00:00:00

abstract：:Decisions as to the disposition of swine carcasses with lesions attributable to a mycobacterial infection are based upon a lesion criterion which is used as a diagnostic test by federal meat inspectors. Using this criterion, a federal meat inspector classified 58 of 100 pigs as "passed for cooking" and the other 42 pi...

journal_title：Applied and environmental microbiology

authors： Brown J,Neuman MA

更新日期：1979-04-01 00:00:00

abstract：:Anti-Helicobacter pylori activities were determined by agar dilution, confocal laser scanning microscopy, and cell proliferation assays following treatment with various grape extracts. Muscadine grape skin possessed the strongest activity, followed by grape synergy (skin and seed) and seed, suggesting that higher phen...

journal_title：Applied and environmental microbiology

authors： Brown JC,Huang G,Haley-Zitlin V,Jiang X

更新日期：2009-02-01 00:00:00

abstract：:Medicinal plants are valuable resources of natural antimicrobial materials. A novel small protein with antimicrobial activities, designated LJAMP1, was purified from the seeds of a medicinal herb, motherwort (Leonurus japonicus Houtt). LJAMP1 is a heat-stable protein with a molecular mass of 7.8 kDa and a determined i...

journal_title：Applied and environmental microbiology

authors： Yang X,Xiao Y,Wang X,Pei Y

更新日期：2007-02-01 00:00:00

abstract：:Zoothamnium niveum is a giant, colonial marine ciliate from sulfide-rich habitats obligatorily covered with chemoautotrophic, sulfide-oxidizing bacteria which appear as coccoid rods and rods with a series of intermediate shapes. Comparative 16S rRNA gene sequence analysis and fluorescence in situ hybridization showed ...

journal_title：Applied and environmental microbiology

authors： Rinke C,Schmitz-Esser S,Stoecker K,Nussbaumer AD,Molnár DA,Vanura K,Wagner M,Horn M,Ott JA,Bright M

更新日期：2006-03-01 00:00:00

abstract：:A restriction fragment length polymorphism analysis of nuclear ribosomal RNA genes (rDNA) was used to measure the amount and distribution of genetic variability in populations of the Japanese pear pathotype of Alternaria alternata on both micro- and macrogeographical scales. A total of 322 isolates were obtained from ...

journal_title：Applied and environmental microbiology

authors： Adachi Y,Watanabe H,Tanabe K,Doke N,Nishimura S,Tsuge T

更新日期：1993-10-01 00:00:00

abstract：:Noroviruses, which are members of the Caliciviridae family, represent the leading cause of nonbacterial gastroenteritis in developed countries; such norovirus infections result in high economic costs for health protection. Person-to-person contact, contaminated water, and foods, especially raw shellfish, vegetables, a...

journal_title：Applied and environmental microbiology

authors： Buckow R,Isbarn S,Knorr D,Heinz V,Lehmacher A

更新日期：2008-02-01 00:00:00

abstract：:Q fever, caused by Coxiella burnetii, is a zoonosis with a worldwide distribution. A large rural area in the southeast of the Netherlands was heavily affected by Q fever between 2007 and 2009. This initiated the development of a robust and internally controlled multiplex quantitative PCR (qPCR) assay for the detection...

journal_title：Applied and environmental microbiology

authors： de Bruin A,de Groot A,de Heer L,Bok J,Wielinga PR,Hamans M,van Rotterdam BJ,Janse I

更新日期：2011-09-01 00:00:00

abstract：:Bacterial growth occurs in noncarbonated natural mineral waters a few days after filling and storage at room temperature, a phenomenon known for more than 40 years. Using the full-cycle rRNA approach, we monitored the development of the planktonic bacterial community in a noncarbonated natural mineral water after bott...

journal_title：Applied and environmental microbiology

authors： Loy A,Beisker W,Meier H

更新日期：2005-07-01 00:00:00

abstract：:Examination of cucumber roots (Cucumis sativus L.) grown in bark compost media and of the surrounding edaphic substrate showed profiles of polar lipid fatty acids commonly found in bacteria. The composition of fatty acids in these profiles differed significantly between roots grown in a medium naturally suppressive to...

journal_title：Applied and environmental microbiology

authors： Tunlid A,Hoitink HA,Low C,White DC

更新日期：1989-06-01 00:00:00

abstract：:The first inducible Arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-D-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans, is described here. Nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system will allow th...

journal_title：Applied and environmental microbiology

authors： Sandu C,Chiribau CB,Sachelaru P,Brandsch R

更新日期：2005-12-01 00:00:00

abstract：:The biological succession that occurs during the first year of life in the gut of infants in Western countries is broadly predictable in terms of the increasing complexity of the composition of microbiotas. Less information is available about microbiotas in Asian countries, where environmental, nutritional, and cultur...

journal_title：Applied and environmental microbiology

authors： Lawley B,Otal A,Moloney-Geany K,Diana A,Houghton L,Heath AM,Taylor RW,Tannock GW

更新日期：2019-09-17 00:00:00

abstract：:Rhizobiophage V, isolated from soil in the vicinity of soybean roots, was strongly lytic on Bradyrhizobium japonicum 123B (USDA 123) but only mildly lytic on strain L4-4, a chemically induced small-colony mutant of 123. Numerous bacteriophage-resistant variants were isolated from L4-4 infected with phage V; two were s...

journal_title：Applied and environmental microbiology

authors： Abebe HM,Sadowsky MJ,Kinkle BK,Schmidt EL

更新日期：1992-10-01 00:00:00

abstract：:Yeasts able to grow on d-xylose were screened for the ability to hydrolyze xylan. Xylanase activity was found to be rare; a total of only 19 of more than 250 strains yielded a positive test result. The activity was localized largely in the genus Cryptococcus and in Pichia stipitis and its anamorph Candida shehatae. Th...

journal_title：Applied and environmental microbiology

authors： Lee H,Biely P,Latta RK,Barbosa MF,Schneider H

更新日期：1986-08-01 00:00:00

abstract：:Virulence gene profiles of atypical enteropathogenic Escherichia coli (aEPEC) and Shiga toxin-producing E. coli (STEC) from cattle, sheep, and humans were examined to determine the relationship between pathotypes. Shared virulence factors (intimin, EHEC hemolysin, serine protease, and a type II secretion system) were ...

journal_title：Applied and environmental microbiology

authors： Cookson AL,Cao M,Bennett J,Nicol C,Thomson-Carter F,Attwood GT

更新日期：2010-06-01 00:00:00

abstract：:Small-subunit (SSU) ribosomal DNA (rDNA) gene clusters are phylogenetically related sets of SSU rRNA genes, commonly encountered in genes amplified from natural populations. Genetic variability in gene clusters could result from artifacts (polymerase error or PCR chimera formation), microevolution (variation among rrn...

journal_title：Applied and environmental microbiology

authors： Field KG,Gordon D,Wright T,Rappé M,Urback E,Vergin K,Giovannoni SJ

更新日期：1997-01-01 00:00:00

abstract：:This work describes the purification and characterization of propionicin F, the first bacteriocin isolated from Propionibacterium freudenreichii. The bacteriocin has a bactericidal activity and is only active against strains of P. freudenreichii. Propionicin F appears to be formed through a processing pathway new to b...

journal_title：Applied and environmental microbiology

authors： Brede DA,Faye T,Johnsborg O,Odegård I,Nes IF,Holo H

更新日期：2004-12-01 00:00:00