Physical and transcriptional map of an aflatoxin gene cluster in Aspergillus parasiticus and functional disruption of a gene involved early in the aflatoxin pathway.

abstract：:Thirty-two chemoheterotrophic bacteria were isolated from unsaturated subsurface soil samples obtained from ca. 70 m below land surface in a high desert in southeastern Idaho. Most isolates were gram positive (84%) and strict aerobes (79%). Acridine orange direct counts of microbes in one subsurface sample showed lowe...

journal_title：Applied and environmental microbiology

authors： Colwell FS

更新日期：1989-09-01 00:00:00

abstract：:The anti-norovirus (anti-NoV) effect of grape seed extract (GSE) was examined by plaque assay for murine norovirus 1 (MNV-1), cell-binding reverse transcription-PCR for human NoV GII.4, and saliva-binding enzyme-linked immunosorbent assay for human NoV GII.4 P particles, with or without the presence of interfering sub...

journal_title：Applied and environmental microbiology

authors： Li D,Baert L,Zhang D,Xia M,Zhong W,Van Coillie E,Jiang X,Uyttendaele M

更新日期：2012-11-01 00:00:00

abstract：:The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved ...

journal_title：Applied and environmental microbiology

authors： Bachmann H,Santos F,Kleerebezem M,van Hylckama Vlieg JE

更新日期：2007-07-01 00:00:00

abstract：:Marine microbial communities often contain multiple closely related phylogenetic clades, but in many cases, it is still unclear what physiological traits differentiate these putative ecotypes. The numerically abundant marine cyanobacterium Synechococcus can be divided into at least 14 clades. In order to better unders...

journal_title：Applied and environmental microbiology

authors： Ahlgren NA,Rocap G

更新日期：2006-11-01 00:00:00

abstract：:Synthetic deoxyoligonucleotide probes that hybridized against the region at positions 455 through 480 of 16S rRNA were developed for the identification of all five Xenorhabdus species. Sequence variation in the respective rRNA region between two strains of Xenorhabdus luminescens in addition allowed the construction o...

journal_title：Applied and environmental microbiology

authors： Pütz J,Meinert F,Wyss U,Ehlers RU,Stackebrandt E

更新日期：1990-01-01 00:00:00

abstract：:Ergosterol, a fungus-specific sterol enriched in cell plasma membranes, is an effective antifungal drug target. However, current knowledge of the ergosterol biosynthesis process in the saprophytic human fungal pathogen Aspergillus fumigatus remains limited. In this study, we found that two endoplasmic reticulum-locali...

journal_title：Applied and environmental microbiology

authors： Long N,Xu X,Zeng Q,Sang H,Lu L

更新日期：2017-02-01 00:00:00

abstract：:Rapid pathogen detection is crucial for the timely introduction of therapeutics. Two groups (one in the United Kingdom and one in the United States) independently evaluated inhibitor-resistant PCR reagents for the direct testing of substrates. In the United Kingdom, a multiplexed Bacillus anthracis (target) and Bacill...

journal_title：Applied and environmental microbiology

authors： Minogue TD,Rachwal PA,Trombley Hall A,Koehler JW,Weller SA

更新日期：2014-02-01 00:00:00

abstract：:Fermentative production of succinic acid from glucose by Escherichia coli was significantly increased by overexpression of phosphoenolpyruvate carboxylase. In contrast, overexpression of phosphoenolpyruvate carboxykinase had no effect. Under optimized conditions, induction of the carboxylase resulted in a 3.5-fold inc...

journal_title：Applied and environmental microbiology

authors： Millard CS,Chao YP,Liao JC,Donnelly MI

更新日期：1996-05-01 00:00:00

abstract：:Chitin is an abundant biopolymer whose degradation is mediated primarily by bacterial chitinases. We developed a degenerate PCR primer set to amplify a approximately 900-bp fragment of family 18, group I chitinase genes and used it to retrieve these gene fragments from environmental samples. Clone libraries of presump...

journal_title：Applied and environmental microbiology

authors： LeCleir GR,Buchan A,Hollibaugh JT

更新日期：2004-12-01 00:00:00

abstract：:Gas chromatographic studies showed that nitrous oxide was produced in each instance when sterilized (autoclaved) soil was incubated after treatment with ammonium sulfate and inoculation with pure cultures of ammonia-oxidizing chemoautotrophic microorganisms (strains of Nitrosomonas, Nitrosospira, and Nitrosolobus). Pr...

journal_title：Applied and environmental microbiology

authors： Blackmer AM,Bremner JM,Schmidt EL

更新日期：1980-12-01 00:00:00

abstract：UNLABELLED:Raman spectroscopy and phase-contrast microscopy were used to examine calcium dipicolinate (CaDPA) levels and rates of nutrient and nonnutrient germination of multiple individual Bacillus subtilis spores treated with cold atmospheric plasma (CAP). Major results for this work include the following: (i) >5 log...

journal_title：Applied and environmental microbiology

authors： Wang S,Doona CJ,Setlow P,Li YQ

更新日期：2016-09-16 00:00:00

abstract：:The single glucosyltransferase (GTF) of Streptococcus gordonii Challis CH1 makes alpha 1,3- and alpha 1,6-linked glucans from sucrose. The GTF carboxyl-terminal region has six direct repeats thought to be involved in glucan binding. Strains with defined mutations in this region have been described recently (M. M. Vick...

journal_title：Applied and environmental microbiology

authors： Vickerman MM,Clewell DB

更新日期：1997-05-01 00:00:00

abstract：:Escherichia coli O157:H7 strains can be classified into different genotypes based on the presence of specific Shiga toxin-encoding bacteriophage insertion sites. Certain O157:H7 genotypes predominate among human clinical cases (clinical genotypes), while others are more frequently found in bovines (bovine-biased genot...

journal_title：Applied and environmental microbiology

authors： Vanaja SK,Springman AC,Besser TE,Whittam TS,Manning SD

更新日期：2010-01-01 00:00:00

abstract：:In this study, the inactivating properties of liquid hydrogen peroxide (L-H(2)O(2)), vaporized hydrogen peroxide (V-H(2)O(2)), UV light, and a combination of V-H(2)O(2) and UV light were tested on murine norovirus 1 (MNV-1) and bacteriophages (φX174 and B40-8) as models for human noroviruses. Disinfection of surfaces ...

journal_title：Applied and environmental microbiology

authors： Li D,Baert L,De Jonghe M,Van Coillie E,Ryckeboer J,Devlieghere F,Uyttendaele M

更新日期：2011-02-01 00:00:00

abstract：:A monoclonal antibody designated 302 beta that is specific for the beta subunit of the oxygenase component (ISPTOL) of toluene dioxygenase from Pseudomonas putida F1 was used to prepare an immunoaffinity column. ISPTOL in cell extracts of Escherichia coli JM109(pDTG611) bound to the column, and an enzyme-linked immuno...

journal_title：Applied and environmental microbiology

authors： Lynch NA,Jiang H,Gibson DT

更新日期：1996-06-01 00:00:00

abstract：:Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. in cooling tower waters. The procedure was quicker and less labor intensive than fluorescent microscopy. The use of these procedures also identified qualitative differences, perhaps ...

journal_title：Applied and environmental microbiology

authors： Tyndall RL,Hand RE Jr,Mann RC,Evans C,Jernigan R

更新日期：1985-04-01 00:00:00

abstract：:Gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy were used to study the metabolism of deuterated n-alkanes (C6 to C12) and 1-13C-labeled n-hexane by a highly enriched sulfate-reducing bacterial culture. All substrates were activated via fumarate addition to form the corresponding alkyls...

journal_title：Applied and environmental microbiology

authors： Davidova IA,Gieg LM,Nanny M,Kropp KG,Suflita JM

更新日期：2005-12-01 00:00:00

abstract：:beta-d-Mannanase from Bacillus pumilus was purified into two components, A and B, which exhibited homogeneity on polyacrylamide gel electrophoresis. These components had molecular weights of 55,000 (A) and 37,000 (B); carbohydrate contents of 15.3% (A) and 7.2% (B); specific activities of 78 (A) and 1,616 (B) U/mg; pH...

journal_title：Applied and environmental microbiology

authors： Araujo A,Ward OP

更新日期：1990-06-01 00:00:00

abstract：:Bacterial precipitation of barite (BaSO(4)) under laboratory conditions is reported for the first time. The bacterium Myxococcus xanthus was cultivated in a solid medium with a diluted solution of barium chloride. Crystallization occurred as a result of the presence of live bacteria and the bacterial metabolic activit...

journal_title：Applied and environmental microbiology

authors： González-Muñoz MT,Fernández-Luque B,Martínez-Ruiz F,Ben Chekroun K,Arias JM,Rodríguez-Gallego M,Martínez-Cañamero M,de Linares C,Paytan A

更新日期：2003-09-01 00:00:00

abstract：:Recovery results of Staphylococcus aureus ATCC 6538 treated with phenolics and quaternary ammonium compounds on Dey and Engley (D/E) neutralizing medium at various time intervals were compared by the use of two commonly used media. Two recovery processes were utilized. In one, the chemically treated organisms were pla...

journal_title：Applied and environmental microbiology

authors： Dey BP,Engley FB Jr

更新日期：1983-05-01 00:00:00

abstract：:The ability of certain oral biofilm bacteria to moderate pH through arginine metabolism by the arginine deiminase system (ADS) is a deterrent to the development of dental caries. Here, we characterize a novel Streptococcus strain, designated strain A12, isolated from supragingival dental plaque of a caries-free indivi...

journal_title：Applied and environmental microbiology

authors： Huang X,Palmer SR,Ahn SJ,Richards VP,Williams ML,Nascimento MM,Burne RA

更新日期：2016-01-29 00:00:00

abstract：:Examination of cucumber roots (Cucumis sativus L.) grown in bark compost media and of the surrounding edaphic substrate showed profiles of polar lipid fatty acids commonly found in bacteria. The composition of fatty acids in these profiles differed significantly between roots grown in a medium naturally suppressive to...

journal_title：Applied and environmental microbiology

authors： Tunlid A,Hoitink HA,Low C,White DC

更新日期：1989-06-01 00:00:00

abstract：:The goal of this field study was to provide insight into three distinct populations of microorganisms involved in in situ metabolism of phenol. Our approach measured 13CO2 respired from [13C]phenol and stable isotope probing (SIP) of soil DNA at an agricultural field site. Traditionally, SIP-based investigations have ...

journal_title：Applied and environmental microbiology

authors： DeRito CM,Pumphrey GM,Madsen EL

更新日期：2005-12-01 00:00:00

abstract：:Beef carcass sponge samples collected from July to August 1999 at four large processing plants in the United States were surveyed for the presence of non-O157 Shiga toxin-producing Escherichia coli (STEC). Twenty-eight (93%) of 30 single-source lots surveyed included at least one sample containing non-O157 STEC. Of 33...

journal_title：Applied and environmental microbiology

authors： Arthur TM,Barkocy-Gallagher GA,Rivera-Betancourt M,Koohmaraie M

更新日期：2002-10-01 00:00:00

abstract：:A selective procedure was used to isolate pectinolytic intestinal bacteria from human subjects. The three isolates with the greatest pectinolytic activity utilized pectin and a few related compounds as fermentable substrates for growth but did not utilize any other compound tested. Thus, their substrate utilization pa...

journal_title：Applied and environmental microbiology

authors： Jensen NS,Canale-Parola E

更新日期：1985-07-01 00:00:00

abstract：:The widespread use and relative persistence of s-triazine compounds such as atrazine and simazine have led to increasing concern about environmental contamination by these compounds. Few microbial isolates capable of transforming substituted s-triazines have been identified. Rhodococcus corallinus NRRL B-15444 has pre...

journal_title：Applied and environmental microbiology

authors： Mulbry WW

更新日期：1994-02-01 00:00:00

abstract：:The diversity of the predominant bacteria in the human gastrointestinal tract was studied by using 16S rRNA-based approaches. PCR amplicons of the V6 to V8 regions of fecal 16S rRNA and ribosomal DNA (rDNA) were analyzed by temperature gradient gel electrophoresis (TGGE). TGGE of fecal 16S rDNA amplicons from 16 indiv...

journal_title：Applied and environmental microbiology

authors： Zoetendal EG,Akkermans AD,De Vos WM

更新日期：1998-10-01 00:00:00

abstract：:Demineralization reduced heat resistance of B. subtilis spores, but the pattern and magnitude of the reduction depended on sporulation temperature and on heating menstruum pH. The differences in heat resistance of native spores caused by sporulation temperature almost disappeared after demineralization. Demineralized ...

journal_title：Applied and environmental microbiology

authors： Palop A,Sala FJ,Condón S

更新日期：1999-03-01 00:00:00

abstract：:The PCR technique has potential for use in detection of low concentrations of airborne microorganisms. In this study, the sensitivity of PCR and its susceptibility to environmental interference were assessed with Escherichia coli DH1 as the target organism. Air samples, containing environmental bioaerosols, were colle...

journal_title：Applied and environmental microbiology

authors： Alvarez AJ,Buttner MP,Stetzenbach LD

更新日期：1995-10-01 00:00:00

abstract：:Germlings of Botrytis cinerea, an important fungal pathogen of plants, produce an extracellular matrix (ECM), or ensheathing film, that serves, in part, in their attachment (R. P. Doss, et al., Appl. Environ. Microbiol. 61:260-265, 1995). The composition of this film has been ascertained by using samples obtained by g...

journal_title：Applied and environmental microbiology

authors： Doss RP

更新日期：1999-02-01 00:00:00