Development and application of oligonucleotide probes for molecular identification of Xenorhabdus species.

abstract：:Vegetative cells of Bacillus cereus were subjected to low doses of nisin (0.06 microg/ml) and mild pulsed-electric field treatment (16.7 kV/cm, 50 pulses each of 2-micros duration). Combining both treatments resulted in a reduction of 1.8 log units more than the sum of the reductions obtained with the single treatment...

journal_title：Applied and environmental microbiology

authors： Pol IE,Mastwijk HC,Bartels PV,Smid EJ

更新日期：2000-01-01 00:00:00

abstract：:An assay has been developed to detect bacterial glycosidases in colonies grown on the surface of agar plates. Advantages of this technique over previously described methods include elimination of the need for replica plating, better visualization of chromagenic reaction products, and a simple permeabilization step to ...

journal_title：Applied and environmental microbiology

authors： Paoni NF,Arroyo RL

更新日期：1984-01-01 00:00:00

abstract：:Pure cultures of Geobacter sulfurreducens and other Fe(III)-reducing bacteria accumulated hydrogen to partial pressures of 5 to 70 Pa with acetate, butyrate, benzoate, ethanol, lactate, or glucose as the electron donor if electron release to an acceptor was limiting. G. sulfurreducens coupled acetate oxidation with el...

journal_title：Applied and environmental microbiology

authors： Cord-Ruwisch R,Lovley DR,Schink B

更新日期：1998-06-01 00:00:00

abstract：:Currently only five (SEA-SEE) out of 27 known staphylococcal enterotoxins can be analyzed using commercially available kits.Six genes (seg, sei, sem, sen, seo, and seu), encoding putative and undetectable enterotoxins, are located on the enterotoxin gene cluster (egc) which is part of the Staphylococcus aureus genomic...

journal_title：Applied and environmental microbiology

authors： Schwendimann L,Merda D,Berger T,Denayer S,Feraudet Tarisse C,Kläui AJ,Messio S,Mistou MY,Nia Y,Hennekinne JA,Graber HU

更新日期：2020-12-18 00:00:00

abstract：:IncQ marker plasmids were previously constructed to enable the analysis of the survival of populations of Pseudomonas putida released into lake water (C. Winstanley, J. A. W. Morgan, R. W. Pickup, J. G. Jones, and J. R. Saunders, Appl. Environ. Microbiol. 55:771-777, 1989). We constructed equivalent IncP plasmids, pLV...

journal_title：Applied and environmental microbiology

authors： Winstanley C,Morgan JA,Pickup RW,Saunders JR

更新日期：1991-07-01 00:00:00

abstract：:A polyphasic approach involving cultivation, direct viable counts, rRNA-based phylogenetic classification, and in situ probing was applied for the characterization of the dominant microbial population in a municipal drinking water distribution system. A total of 234 bacterial strains cultivated on R2A medium were scre...

journal_title：Applied and environmental microbiology

authors： Kalmbach S,Manz W,Szewzyk U

更新日期：1997-11-01 00:00:00

abstract：:A device for sampling at depths of up to 6,000 m is described in which 3 liters of seawater is concentrated over a Nucleopore filter to about 13 ml and retrieved under in situ pressure and temperature. Subsamples can be withdrawn into transfer units that are equipped with individual gas accumulators for preventing los...

journal_title：Applied and environmental microbiology

authors： Jannasch HW,Wirsen CO

更新日期：1977-03-01 00:00:00

abstract：:A modified glucoamylase gene (glaA) with an extra Thr- and Ser-rich Gp-I domain (T. Semimaru, M. Goto, K. Furukawa, and S. Hayashida, Appl. Environ. Microbiol. 61:2885-2890, 1995) was introduced into a mutant parental host, Aspergillus awamori var. kawachi, in which the original glaA gene had been completely deleted a...

journal_title：Applied and environmental microbiology

authors： Goto M,Ekino K,Furukawa K

更新日期：1997-07-01 00:00:00

abstract：:PCR-based methods have been developed to rapidly screen for Legionella pneumophila in water as an alternative to time-consuming culture techniques. However, these methods fail to discriminate between live and dead bacteria. Here, we report a viability assay (viability PCR [v-PCR]) for L. pneumophila that combines ethi...

journal_title：Applied and environmental microbiology

authors： Delgado-Viscogliosi P,Solignac L,Delattre JM

更新日期：2009-06-01 00:00:00

abstract：:The rep-PCR DNA fingerprint technique, which uses repetitive intergenic DNA sequences, was investigated as a way to differentiate between human and animal sources of fecal pollution. BOX and REP primers were used to generate DNA fingerprints from Escherichia coli strains isolated from human and animal sources (geese, ...

journal_title：Applied and environmental microbiology

authors： Dombek PE,Johnson LK,Zimmerley ST,Sadowsky MJ

更新日期：2000-06-01 00:00:00

abstract：:Geographical and seasonal variation in the incidence and prevalence of Campylobacter jejuni and C. coli in housed broiler flocks reared in Great Britain in 2004 to 2006 was investigated in this study. Ceca (30) from 797 flocks, not subject to prior partial depopulation and reared on 211 farms, were examined individual...

journal_title：Applied and environmental microbiology

authors： Jorgensen F,Ellis-Iversen J,Rushton S,Bull SA,Harris SA,Bryan SJ,Gonzalez A,Humphrey TJ

更新日期：2011-06-01 00:00:00

abstract：:Of 70 pure microbial cultures isolated from aquatic habitats, soil, and air according to the ability to lyse live blue-green algae, 62 were fungi representing the genera Acremonium, Emericellopsis, and Verticillium. Algal-lysing fungi were isolated from all habitat types sampled. The remaining isolates comprised four ...

journal_title：Applied and environmental microbiology

authors： Redhead K,Wright SJ

更新日期：1978-05-01 00:00:00

abstract：:Fermentative production of succinic acid from glucose by Escherichia coli was significantly increased by overexpression of phosphoenolpyruvate carboxylase. In contrast, overexpression of phosphoenolpyruvate carboxykinase had no effect. Under optimized conditions, induction of the carboxylase resulted in a 3.5-fold inc...

journal_title：Applied and environmental microbiology

authors： Millard CS,Chao YP,Liao JC,Donnelly MI

更新日期：1996-05-01 00:00:00

abstract：:Male-specific RNA coliphages (F-RNA coliphages) have been proposed as a potential viral indicator of fecal contamination in water and foods because they are easy to culture and are a normal component of the mammalian gut flora. F-RNA coliphage plaque numbers are typically obtained by directly plating a 10-fold dilutio...

journal_title：Applied and environmental microbiology

authors： Jones TH,Johns MW

更新日期：2009-10-01 00:00:00

abstract：:The only major structural protein (35 kDa) of the lactococcal small isometric-headed bacteriophage ul36, a member of the P335 species, was isolated from a preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Monoclonal antibodies (MAbs) were raised against the denatured 35-kDa protein. Six MAbs...

journal_title：Applied and environmental microbiology

authors： Moineau S,Bernier D,Jobin M,Hébert J,Klaenhammer TR,Pandian S

更新日期：1993-07-01 00:00:00

abstract：:A d-xylose reductase mutant of Pachysolen tannophilus was isolated on the basis of its poor growth on d-xylose but normal growth on xylitol and d-glucose. Fractionation of cell extracts indicated that the mutant was deficient in d-xylose reductase activity that used NADPH exclusively as a cofactor, but not in activity...

journal_title：Applied and environmental microbiology

authors： Schneider H,Lee H,Barbosa Mde F,Kubicek CP,James AP

更新日期：1989-11-01 00:00:00

abstract：:Biocontrol agents generally do not perform well enough under field conditions to compete with chemical fungicides. We determined whether transgenic strain SJ3-4 of Trichoderma atroviride, which expresses the Aspergillus niger glucose oxidase-encoding gene, goxA, under a homologous chitinase (nag1) promoter had increas...

journal_title：Applied and environmental microbiology

authors： Brunner K,Zeilinger S,Ciliento R,Woo SL,Lorito M,Kubicek CP,Mach RL

更新日期：2005-07-01 00:00:00

abstract：:A mixed population, enriched and established in a defined medium, from a sewage sludge inoculum was capable of complete mineralization of 4-chlorobenzoate. An organism, identified as Arthrobacter sp., was isolated from the consortium and shown to be capable of utilizing 4-chlorobenzoate as the sole carbon and energy s...

journal_title：Applied and environmental microbiology

authors： Marks TS,Smith AR,Quirk AV

更新日期：1984-11-01 00:00:00

abstract：:Tellurium, a metalloid belonging to group 16 of the periodic table, displays very interesting physical and chemical properties and lately has attracted significant attention for its use in nanotechnology. In this context, the use of microorganisms for synthesizing nanostructures emerges as an eco-friendly and exciting...

journal_title：Applied and environmental microbiology

authors： Pugin B,Cornejo FA,Muñoz-Díaz P,Muñoz-Villagrán CM,Vargas-Pérez JI,Arenas FA,Vásquez CC

更新日期：2014-11-01 00:00:00

abstract：:Inactivation of aflatoxin B1 was studied by using gamma radiation and hydrogen peroxide. A 100-krad dose of gamma radiation was sufficient to inactivate 50 micrograms of aflatoxin B1 in the presence of 5% hydrogen peroxide, and 400 krad was required for total degradation of 100 micrograms of aflatoxin in the same syst...

journal_title：Applied and environmental microbiology

authors： Patel UD,Govindarajan P,Dave PJ

更新日期：1989-02-01 00:00:00

abstract：:The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594. Results confirmed that the DNA sequence of the 359-bp...

journal_title：Applied and environmental microbiology

authors： Ben-Porath J,Zehr JP

更新日期：1994-03-01 00:00:00

abstract：:Gonometa podocarpi is an important pest of several species of pine in East Africa, and large numbers of trees in plantations in Kenya were partially or completely defoliated by the larval stage of this insect. After the infestation in the Mt. Elgon region, large numbers of dead and moribund larvae were found on the gr...

journal_title：Applied and environmental microbiology

authors： Moore NF,Armstrong T,Green B,Brown D,Hibbin J,Kelly DC,Tinsley TW,Aloo TC

更新日期：1981-08-01 00:00:00

abstract：:Epilithic bacteria were isolated nonselectively from riverbed stones and examined by gel zymography for their ability to produce alkylsulfatase (AS) enzymes and thus to metabolize alkyl sulfate surfactants such as sodium dodecyl sulfate. The percentages of AS+ isolates from stone epilithon at five sites from the sourc...

journal_title：Applied and environmental microbiology

authors： Anderson DJ,Day MJ,Russell NJ,White GF

更新日期：1988-02-01 00:00:00

abstract：:During malolactic fermentation in wine by Oenococcus oeni, the degradation of citric acid was delayed compared to the degradation of malic acid. The maximum concentration of diacetyl, an intermediary compound in the citric acid metabolism with a buttery or nutty flavor, coincided with the exhaustion of malic acid in t...

journal_title：Applied and environmental microbiology

authors： Nielsen JC,Richelieu M

更新日期：1999-02-01 00:00:00

abstract：:Strains of Xylella fastidiosa isolated from grape, almond, maple, and oleander were characterized by enterobacterial repetitive intergenic consensus sequence-, repetitive extragenic palindromic element (REP)-, and random amplified polymorphic DNA (RAPD)-PCR; contour-clamped homogeneous electric field (CHEF) gel electr...

journal_title：Applied and environmental microbiology

authors： Hendson M,Purcell AH,Chen D,Smart C,Guilhabert M,Kirkpatrick B

更新日期：2001-02-01 00:00:00

abstract：:Vibrio parahaemolyticus and Vibrio vulnificus, which are native to estuaries globally, are agents of seafood-borne or wound infections, both potentially fatal. Like all vibrios autochthonous to coastal regions, their abundance varies with changes in environmental parameters. Sea surface temperature (SST), sea surface ...

journal_title：Applied and environmental microbiology

authors： Johnson CN,Bowers JC,Griffitt KJ,Molina V,Clostio RW,Pei S,Laws E,Paranjpye RN,Strom MS,Chen A,Hasan NA,Huq A,Noriea NF 3rd,Grimes DJ,Colwell RR

更新日期：2012-10-01 00:00:00

abstract：:Sphingomonas paucimobilis B90A is able to degrade the alpha-, beta-, gamma-, and delta-isomers of hexachlorocyclohexane (HCH). It contains the genes linA, linB, linC, linD, linE, and linR, which have been implicated in HCH degradation. In this study, dynamic expression of the lin genes was measured in chemostat-grown ...

journal_title：Applied and environmental microbiology

authors： Suar M,van der Meer JR,Lawlor K,Holliger C,Lal R

更新日期：2004-11-01 00:00:00

abstract：:We have analyzed the impact of surface-to-volume ratio on final bacterial concentrations after batch growth. We examined six bottle sizes (20 to 1,000 ml) using three independent enumeration methods to quantify growth. We found no evidence of a so-called volumetric bottle effect, thus contradicting numerous previous r...

journal_title：Applied and environmental microbiology

authors： Hammes F,Vital M,Egli T

更新日期：2010-02-01 00:00:00

abstract：:Mass spectral analysis of T-2 toxin formed during the growth of Fusarium sporotrichioides (ATCC 24043) in the presence of H(2)O showed incorporation of up to three O atoms per toxin molecule. The carbonyl oxygens of the acetates at C-4 and C-15 and of the isovalerate at C-8 were derived from H(2)O. Toxin formed in the...

journal_title：Applied and environmental microbiology

authors： Desjardins AE,Plattner RD,Vanmiddlesworth F

更新日期：1986-03-01 00:00:00

abstract：:The combination of ecological diversity with genetic and experimental tractability makes Drosophila a powerful model for the study of animal-associated microbial communities. Despite the known importance of yeasts in Drosophila physiology, behavior, and fitness, most recent work has focused on Drosophila-bacterial int...

journal_title：Applied and environmental microbiology

authors： Chandler JA,Eisen JA,Kopp A

更新日期：2012-10-01 00:00:00