Critical evaluation of the volumetric "bottle effect" on microbial batch growth.

abstract：:Anthrax is a fatal infection of humans and livestock that is caused by the gram-positive bacterium Bacillus anthracis. The virulent strains of B. anthracis are encapsulated and toxigenic. In this paper we describe the development of a PCR technique for identifying spores of B. anthracis. Two 20-mer oligonucleotide pri...

journal_title：Applied and environmental microbiology

authors： Reif TC,Johns M,Pillai SD,Carl M

更新日期：1994-05-01 00:00:00

abstract：:Halophenols such as 2,4-dibromophenol (DBP) occur naturally in some marine sediments, as a consequence of various animal and algal activities. In an earlier study, DBP was observed in the burrow microenvironment of the hemichordate Saccoglossus kowalewskii. At the concentrations found in the burrow lining, aerobic res...

journal_title：Applied and environmental microbiology

authors： King GM

更新日期：1988-12-01 00:00:00

abstract：:We examined the competitive relationship between two isolates of denitrifying bacteria, both of which grow well under aerobic conditions but differ in their ability to grow under denitrifying conditions. The growth and persistence of the two isolates, added to sterile soil or added to soil previously colonized by the ...

journal_title：Applied and environmental microbiology

authors： Murray RE,Parsons LL,Smith MS

更新日期：1992-12-01 00:00:00

abstract：:Sporulation of Clostridium botulinum 113B in a complex medium supplemented with certain transition metals (Fe, Mn, Cu, or Zn) at 0.01 to 1.0 mM gave spores that were increased two to sevenfold in their contents of the added metals. The contents of calcium, magnesium, and other metals in the purified spores were relati...

journal_title：Applied and environmental microbiology

authors： Kihm DJ,Hutton MT,Hanlin JH,Johnson EA

更新日期：1990-03-01 00:00:00

abstract：:The phospholipid fatty acid (PLFA) pattern was analyzed in a forest humus and in an arable soil experimentally polluted with Cd, Cu, Ni, Pb, or Zn at different concentrations. In both soil types, there were gradual changes in the PLFA patterns for the different levels of metal contamination. The changes in the forest ...

journal_title：Applied and environmental microbiology

authors： Frostegård A,Tunlid A,Bååth E

更新日期：1993-11-01 00:00:00

abstract：:Two strains of small spirochetes utilizing pectin as sole source of energy for growth were isolated from the rumen of cattle and partially characterized. The pectinolytic enzyme produced by one of the organisms was classified as a poly-1,4-alpha-D-galacturonide lyase (EC 4.2.2.2). A pectinesterase (EC 3.1.1.11) was al...

journal_title：Applied and environmental microbiology

authors： Ziołecki A,Wojciechowicz M

更新日期：1980-04-01 00:00:00

abstract：:The temporal and spatial distribution of Salmonella contamination in the coastal waters of Galicia (northwestern Spain) relative to contamination events with different environmental factors (temperature, wind, hours of sunlight, rainfall, and river flow) were investigated over a 4-year period. Salmonellae were isolate...

journal_title：Applied and environmental microbiology

authors： Martinez-Urtaza J,Saco M,de Novoa J,Perez-Piñeiro P,Peiteado J,Lozano-Leon A,Garcia-Martin O

更新日期：2004-04-01 00:00:00

abstract：UNLABELLED:In the summer of 2010, Vibrio parahaemolyticus caused an outbreak in Maryland linked to the consumption of oysters. Strains isolated from both stool and oyster samples were indistinguishable by pulsed-field gel electrophoresis (PFGE). However, the oysters contained other potentially pathogenic V. parahaemoly...

journal_title：Applied and environmental microbiology

authors： Haendiges J,Jones J,Myers RA,Mitchell CS,Butler E,Toro M,Gonzalez-Escalona N

更新日期：2016-05-16 00:00:00

abstract：:A molecular method based on restriction fragment length polymorphism (RFLP) of PCR-amplified fragments of the 23S rRNA gene was designed to rapidly identify Listeria strains to the species level. Two fragments (S1, 460 bp, and S2, 890 bp) were amplified from boiled DNA. S2 was cut with the restriction enzymes XmnI or ...

journal_title：Applied and environmental microbiology

authors： Paillard D,Dubois V,Duran R,Nathier F,Guittet C,Caumette P,Quentin C

更新日期：2003-11-01 00:00:00

abstract：:Primers were designed to target 16S rRNA and nodD genes of Rhizobium leguminosarum from DNA extracted from two different soil types contaminated with Zn applied in sewage sludge. Numbers of rhizobia estimated using 16S rRNA gene copy number showed higher abundance than those estimated by both nodD and the most-probabl...

journal_title：Applied and environmental microbiology

authors： Macdonald CA,Clark IM,Hirsch PR,Zhao FJ,McGrath SP

更新日期：2011-07-01 00:00:00

abstract：:There is growing interest in the application of human-associated fecal source identification quantitative real-time PCR (qPCR) technologies for water quality management. The transition from a research tool to a standardized protocol requires a high degree of confidence in data quality across laboratories. Data quality...

journal_title：Applied and environmental microbiology

authors： Shanks OC,Kelty CA,Oshiro R,Haugland RA,Madi T,Brooks L,Field KG,Sivaganesan M

更新日期：2016-04-18 00:00:00

abstract：:Highly methylated, long-chain celluloses strongly inhibited cellulose degradation by several species of cellulolytic bacteria of ruminal origin. Specifically, the inhibitory effects of methylcellulose on the growth of Ruminococcus flavefaciens FD1 were concentration dependent, with complete inhibition at 0.1% (wt/vol)...

journal_title：Applied and environmental microbiology

authors： Rasmussen MA,Hespell RB,White BA,Bothast RJ

更新日期：1988-04-01 00:00:00

abstract：:The present investigation showed by means of autoradiography that the cyanobacterium Microcystis wesenbergii did not incorporate [H]thymidine at nanomolar concentrations, whereas its associated heterotrophic bacteria appearing in the gelatinous cover of the cyanobacterium became labeled. Several other tested cyaobacte...

journal_title：Applied and environmental microbiology

authors： Bern L

更新日期：1985-01-01 00:00:00

abstract：:The occurrence of 10 pathogens and three fecal indicators was assessed by quantitative PCR in manures of Australian feedlot cattle. Most samples tested positive for one or more pathogens. For the dominant pathogens Campylobacter jejuni, Listeria monocytogenes, Giardia spp., Cryptosporidium spp., and eaeA-positive Esch...

journal_title：Applied and environmental microbiology

authors： Klein M,Brown L,Tucker RW,Ashbolt NJ,Stuetz RM,Roser DJ

更新日期：2010-10-01 00:00:00

abstract：:Two genes involved in aflatoxin B1 (AFB1) biosynthesis in Aspergillus parasiticus, nor-1 and ver-1, were localized to a 35-kb region on one A. parasiticus chromosome and to the genomic DNA fragment carried on a single cosmid, NorA. A physical and transcriptional map of the 35-kb genomic DNA insert in cosmid NorA was p...

journal_title：Applied and environmental microbiology

authors： Trail F,Mahanti N,Rarick M,Mehigh R,Liang SH,Zhou R,Linz JE

更新日期：1995-07-01 00:00:00

abstract：:phi-0303 is a temperate bacteriophage isolated from Lactobacillus helveticus CNRZ 303 strain after mitomycin C induction. In this work, the gene coding for a lytic protein of this bacteriophage was cloned using a library of phi-0303 in Escherichia coli DH5alpha. The lytic activity was detected by its expression, using...

journal_title：Applied and environmental microbiology

authors： Deutsch SM,Guezenec S,Piot M,Foster S,Lortal S

更新日期：2004-01-01 00:00:00

abstract：:A total of four pathways are known for the catabolism by microorganisms of gentisate (2,5-dihydroxybenzoate) and homogentisate (2,5-dihydroxyphenylacetate). Both of these dihydric phenols can be degraded by either a glutathione-dependent or a glutathione-independent reaction sequence. We found that it is not always po...

journal_title：Applied and environmental microbiology

authors： Crawford RL,Frick TD

更新日期：1977-08-01 00:00:00

abstract：:The effect of the washing aid T-128 (generally recognized as safe [GRAS] formulation, composed mainly of phosphoric acid and propylene glycol) on inactivation of Salmonella and Pseudomonas populations in biofilms on stainless steel was evaluated under conditions of increasing organic matter loads in chlorinated wash s...

journal_title：Applied and environmental microbiology

authors： Shen C,Luo Y,Nou X,Bauchan G,Zhou B,Wang Q,Millner P

更新日期：2012-10-01 00:00:00

abstract：:Understanding of functional diversity of microbial populations has lagged description of their molecular diversity. Differences in substrate specificity, kinetics, products, and regulation can dramatically influence phenotypic variation among closely related strains, features that are missed when the strains studied a...

journal_title：Applied and environmental microbiology

authors： Rhodes AN,Fulthorpe RR,Tiedje JM

更新日期：2013-11-01 00:00:00

abstract：:Aquaglyceroporins (GlpFs) that transport glycerol along with water and other uncharged solutes are involved in osmoregulation in myriad species. Fungal species form a large group of eukaryotic organisms, and their GlpFs may be diverse, exhibiting various activities. However, few filamentous fungal GlpFs have been biol...

journal_title：Applied and environmental microbiology

authors： Liu XD,Wei Y,Zhou XY,Pei X,Zhang SH

更新日期：2015-10-01 00:00:00

abstract：:The thermophilic bacterium Bacillus thermoantarcticus produces two exocellular polysaccharides (EPS 1 and EPS 2), which can be obtained from the supernatant of liquid cultures by cold-ethanol precipitation, in yields as high as 400 mg liter(sup-1). The EPS fraction was produced with all substrates tested, although a h...

journal_title：Applied and environmental microbiology

authors： Manca MC,Lama L,Improta R,Esposito E,Gambacorta A,Nicolaus B

更新日期：1996-09-01 00:00:00

abstract：:Heat-treated cells of Rhizobium leguminosarum biovar trifolii strain 162X95 were used to produce monoclonal antibodies (MAbs). The fusion produced three cross-reactive MAbs and eight MAbs specific for the immunizing strain and a group of five other R. trifolii strains from the same geographic region where 162X95 was i...

journal_title：Applied and environmental microbiology

authors： Wright SF

更新日期：1990-07-01 00:00:00

abstract：:Mycosporine-like amino acids (MAAs) are an important class of secondary metabolites known for their protection against UV radiation and other stress factors. Cyanobacteria produce a variety of MAAs, including shinorine, the active ingredient in many sunscreen creams. Bioinformatic analysis of the genome of the soil-dw...

journal_title：Applied and environmental microbiology

authors： Katoch M,Mazmouz R,Chau R,Pearson LA,Pickford R,Neilan BA

更新日期：2016-09-30 00:00:00

abstract：:Cyanobacterial hepatotoxins, microcystins, are specific inhibitors of serine/threonine protein phosphatases and potent tumor promoters. They have caused several poisonings of animals and also pose a health hazard for humans through the use of water for drinking and recreation. Different strains of the same cyanobacter...

journal_title：Applied and environmental microbiology

authors： Rapala J,Sivonen K,Lyra C,Niemelä SI

更新日期：1997-06-01 00:00:00

abstract：:The ferric uptake regulator (Fur) box-like sequence was located upstream of the serine protease-encoding gene (aprII) from a marine bacterium, Alteromonas sp. strain O-7. To clarify whether the production of AprII (the gene product of aprII) is regulated by the environmental iron concentrations, this strain was cultur...

journal_title：Applied and environmental microbiology

authors： Tsujibo H,Miyamoto K,Okamoto T,Orikoshi H,Inamori Y

更新日期：2000-09-01 00:00:00

abstract：:A radiometric method for the detection of Salmonella in foods has been developed which is based on Salmonella poly H agglutinating serum preventing Salmonella from producing 14CO2 from [14C]dulcitol. The method will detect the presence or absence of Salmonella in a product within 30 h compared to 4 to 5 days by routin...

journal_title：Applied and environmental microbiology

authors： Stewart BJ,Eyles MJ,Murrell WG

更新日期：1980-08-01 00:00:00

abstract：:Anaerobic degradation of polycyclic aromatic hydrocarbons has been investigated mostly with naphthalene as a model compound. Naphthalene degradation by sulfate-reducing bacteria proceeds via carboxylation to 2-naphthoic acid, formation of a coenzyme A thioester, and subsequent reduction to 5,6,7,8-tetrahydro-2-naphtho...

journal_title：Applied and environmental microbiology

authors： Weyrauch P,Heker I,Zaytsev AV,von Hagen CA,Arnold ME,Golding BT,Meckenstock RU

更新日期：2020-07-20 00:00:00

abstract：:Quaternary ammonium compounds such as benzalkonium chloride (BC) are widely used as disinfectants in both food processing and medical environments. BC-resistant strains of Listeria monocytogenes have been implicated in multistate outbreaks of listeriosis and have been frequently isolated from food processing plants. H...

journal_title：Applied and environmental microbiology

authors： Elhanafi D,Dutta V,Kathariou S

更新日期：2010-12-01 00:00:00

abstract：:A soy sauce koji mold, Aspergillus flavus var. columnaris Raper and Fennel (ATCC 44310), was treated with UV irradiation to obtain mutant strains possessing high protease activities, high amylase activities, and light-colored conidia. Selected mutant strains were tested for toxicity, and some were found acutely toxic ...

journal_title：Applied and environmental microbiology

authors： Kalayanamitr A,Bhumiratana A,Flegel TW,Glinsukon T,Shinmyo A

更新日期：1987-08-01 00:00:00

abstract：:Reporter gene technology was used to observe the regulation of the alginate biosynthesis gene, algC in a mucoid strain of Pseudomonas aeruginosa in developing and mature biofilms in continuous culture on Teflon and glass substrata. The plasmid pNZ63, carrying an algC-lacZ transcriptional fusion, was shown to not be di...

journal_title：Applied and environmental microbiology

authors： Davies DG,Geesey GG

更新日期：1995-03-01 00:00:00