Characterization of a Nuclear Polyhedrosis Virus Isolated from Diseased Gonometa podocarpi (Lepidoptera:Lasiocampidae).

abstract：:Genetic manipulation of the category B select agents Burkholderia pseudomallei and Burkholderia mallei has been stifled due to the lack of compliant selectable markers. Hence, there is a need for additional select-agent-compliant selectable markers. We engineered a selectable marker based on the gat gene (encoding gly...

journal_title：Applied and environmental microbiology

authors： Norris MH,Kang Y,Lu D,Wilcox BA,Hoang TT

更新日期：2009-10-01 00:00:00

abstract：:Phylogeny and the distribution of symbiotic bacteria in the mixed segment of the wood-eating termite Nasutitermes takasagoensis (Shiraki) were studied. Bacterial 16S rRNA genes (rDNA) were amplified from the mixed segment of the gut by PCR, and two kinds of sequences were identified. The phylogenetic tree was construc...

journal_title：Applied and environmental microbiology

authors： Tokuda G,Yamaoka I,Noda H

更新日期：2000-05-01 00:00:00

abstract：:In raw sludges and in mesophilically and thermophilically digested anaerobic sludges, large variations in numbers of viruses occurred over narrow ranges of numbers of fecal coliforms, total coliforms, and fecal streptococci, demonstrating that the bacteria were poor quantitative reflectors of the numbers of the viruse...

journal_title：Applied and environmental microbiology

authors： Berg G,Berman D

更新日期：1980-02-01 00:00:00

abstract：:The rep-PCR DNA fingerprint technique, which uses repetitive intergenic DNA sequences, was investigated as a way to differentiate between human and animal sources of fecal pollution. BOX and REP primers were used to generate DNA fingerprints from Escherichia coli strains isolated from human and animal sources (geese, ...

journal_title：Applied and environmental microbiology

authors： Dombek PE,Johnson LK,Zimmerley ST,Sadowsky MJ

更新日期：2000-06-01 00:00:00

abstract：:The expression of xenobiotic-degradative genes in indigenous bacteria or in bacteria introduced into an ecosystem is essential for the successful bioremediation of contaminated environments. The maintenance of naphthalene utilization activity is studied in Pseudomonas putida (ATCC 17484) and an Alcaligenes sp. (strain...

journal_title：Applied and environmental microbiology

authors： Guerin WF,Boyd SA

更新日期：1995-11-01 00:00:00

abstract：:Enterotoxigenic Escherichia coli (ETEC) strains that produce immunologically heterogeneous fimbriae and enterotoxins are the primary cause of neonatal diarrhea and postweaning diarrhea in young pigs. A multivalent vaccine inducing protective immunity against ideally all ETEC fimbriae and enterotoxins could be effectiv...

journal_title：Applied and environmental microbiology

authors： Lu T,Moxley RA,Zhang W

更新日期：2019-05-16 00:00:00

abstract：:The U.S. Environmental Protection Agency's information collection rule requires the use of 1MDS electropositive filters for concentrating enteric viruses from water, but unfortunately, these filters are not cost-effective for routine viral monitoring. In this study, an inexpensive electropositive cartridge filter, the...

journal_title：Applied and environmental microbiology

authors： Karim MR,Rhodes ER,Brinkman N,Wymer L,Fout GS

更新日期：2009-04-01 00:00:00

abstract：:A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DN...

journal_title：Applied and environmental microbiology

authors： Datta AR,Wentz BA,Hill WE

更新日期：1987-09-01 00:00:00

abstract：:Infrared spectra of type cultures of the six recognized species of the genus Listeria and of Listeria grayi subsp. murrayi were recorded. By use of a library of 59 spectra, comprising at least six replicates of each type, discrimination by canonical variate analysis of the spectral amplitudes allowed all of the spectr...

journal_title：Applied and environmental microbiology

authors： Holt C,Hirst D,Sutherland A,MacDonald F

更新日期：1995-01-01 00:00:00

abstract：:The biodegradation of tert-butylphenyl diphenyl phosphate (BPDP) was examined in microcosms containing sediment and water from five different ecosystems as part of our studies to elucidate the environmental fate of phosphate ester flame retardants. Biodegradation of [14C]BPDP was monitored in the environmental microco...

journal_title：Applied and environmental microbiology

authors： Heitkamp MA,Freeman JP,Cerniglia CE

更新日期：1986-02-01 00:00:00

abstract：:Processes by which fecal bacteria enter overland flow and their transportation state to surface waters are poorly understood, making the effectiveness of measures designed to intercept this pathway, such as vegetated buffer strips, difficult to predict. Freshly made and aged (up to 30 days) cowpats were exposed to sim...

journal_title：Applied and environmental microbiology

authors： Muirhead RW,Collins RP,Bremer PJ

更新日期：2005-06-01 00:00:00

abstract：:Few studies, if any, have addressed the adhesion of enterococci to the intestinal mucosa and their interference with the adhesion of pathogens, although more than 60% of probiotic preparations in the market contain strains of enterococci. The objective of this study was to investigate if Enterococcus faecium 18C23 has...

journal_title：Applied and environmental microbiology

authors： Jin LZ,Marquardt RR,Zhao X

更新日期：2000-10-01 00:00:00

abstract：:The growth of and production of protease, alpha-amylase, alpha-galactosidase, and lipase by Actinomucor taiwanensis in relation to temperature and relative humidity during the preparation of sufu (Chinese cheese) pehtze were investigated. The incubation temperature, humidity, and cultivation time greatly affected the ...

journal_title：Applied and environmental microbiology

authors： Chou CC,Ho FM,Tsai CS

更新日期：1988-03-01 00:00:00

abstract：:The composition of phospholipids from Mycobacterium convolutum R22 was determined after growth at two temperatures (20 and 30 degrees C) with 1-chlorohexadecane as the substrate. Comparisons were made with the phospholipids of cells grown on n-hexadecane. Phosphatidylinositolmannosides and phosphatidylethanolamine (PE...

journal_title：Applied and environmental microbiology

authors： Murphy GL,Perry JJ

更新日期：1987-01-01 00:00:00

abstract：:Escherichia coli strains harboring deletion mutations of the insecticidal protoxin gene of Bacillus thuringiensis subsp. berliner 1715 were constructed. Although these strains did not produce intact protoxin, cell extracts from one of the mutants were extremely toxic to tobacco hornworm (Manduca sexta) larvae, indicat...

journal_title：Applied and environmental microbiology

authors： Wabiko H,Held GA,Bulla LA Jr

更新日期：1985-03-01 00:00:00

abstract：:Virus adsorption to soils is considered to be the most important factor in removing viruses after land treatment of wastewater. Most of the studies on virus adsorption to soils have utilized poliovirus as the model system. In the present study, comparative adsorption of a number of different types and strains of human...

journal_title：Applied and environmental microbiology

authors： Goyal SM,Gerba CP

更新日期：1979-08-01 00:00:00

abstract：:The 3-hydroxy fatty acids (3-OHFAs) in lipopolysaccharides (LPS) play an important role in determining endotoxin activity, and childhood exposure to endotoxin has recently been associated with reduced risk of atopic diseases. To characterize the 3-OHFAs in house dust (HD), we used gas chromatography-mass spectrometry ...

journal_title：Applied and environmental microbiology

authors： Park JH,Szponar B,Larsson L,Gold DR,Milton DK

更新日期：2004-01-01 00:00:00

abstract：:Flavobacteria are a poorly understood and speciated group of commensal bacteria and opportunistic pathogens. The psychrotroph Flavobacterium psychrophilum is the etiological agent of rainbow trout fry syndrome and bacterial cold water disease, septicemic diseases that heavily impact salmonids. Consequently, two verifi...

journal_title：Applied and environmental microbiology

authors： Crump EM,Perry MB,Clouthier SC,Kay WW

更新日期：2001-02-01 00:00:00

abstract：:Despite the increasing knowledge of Synechococcus spp. and their co-occurring cyanophages in oceanic and coastal water, little is known about their abundance, distribution, and interactions in the Chesapeake Bay estuarine ecosystem. A 5-year interannual survey shows that Synechococcus spp. and their phages are persist...

journal_title：Applied and environmental microbiology

authors： Wang K,Wommack KE,Chen F

更新日期：2011-11-01 00:00:00

abstract：:Gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy were used to study the metabolism of deuterated n-alkanes (C6 to C12) and 1-13C-labeled n-hexane by a highly enriched sulfate-reducing bacterial culture. All substrates were activated via fumarate addition to form the corresponding alkyls...

journal_title：Applied and environmental microbiology

authors： Davidova IA,Gieg LM,Nanny M,Kropp KG,Suflita JM

更新日期：2005-12-01 00:00:00

abstract：:Facultative endosymbionts can benefit insect hosts in a variety of ways, including context-dependent roles, such as providing defense against pathogens. The role of some symbionts in defense may be overlooked, however, when pathogen infection is transient, sporadic, or asymptomatic. The facultative endosymbiont Ricket...

journal_title：Applied and environmental microbiology

authors： Hendry TA,Hunter MS,Baltrus DA

更新日期：2014-12-01 00:00:00

abstract：:Cyanobacteria can synthesize alkanes and alkenes, which are considered to be infrastructure-compatible biofuels. In terms of physiological function, cyanobacterial hydrocarbons are thought to be essential for membrane flexibility for cell division, size, and growth. The genetic basis for the biosynthesis of terminal o...

journal_title：Applied and environmental microbiology

authors： Zhu T,Scalvenzi T,Sassoon N,Lu X,Gugger M

更新日期：2018-06-18 00:00:00

abstract：:"Caldocellum saccharolyticum" is an obligatory anaerobic thermophilic bacterium. A gene from this organism, designated celB, has been cloned in Escherichia coli as part of a bacteriophage lambda gene library. This gene produces a thermostable cellulase that shows both endoglucanase and exoglucanase activities on test ...

journal_title：Applied and environmental microbiology

authors： Saul DJ,Williams LC,Grayling RA,Chamley LW,Love DR,Bergquist PL

更新日期：1990-10-01 00:00:00

abstract：:DNA dot blot hybridizations with a cryV-specific probe and a cryI-specific probe were performed to screen 24 Bacillus thuringiensis strains for their cryV-type (lepidopteran- and coleopteran-specific) and cryI-type (lepidopteran-specific) insecticidal crystal protein gene contents, respectively. The cryV-specific prob...

journal_title：Applied and environmental microbiology

authors： Shin BS,Park SH,Choi SK,Koo BT,Lee ST,Kim JI

更新日期：1995-06-01 00:00:00

abstract：:The electron donor cytochrome b5 (CybE/Cyb5) fuels the activity of the ergosterol biosynthesis-related P450 enzymes/P450s by providing electrons to P450s to promote ergosterol biosynthesis. Previous studies reported that lack of Aspergillus fumigatus (A. fumigatus) CybE reduces the proportion of ergosterol in total st...

journal_title：Applied and environmental microbiology

authors： Zhang C,Ren Y,Gao L,Gu H,Lu L

更新日期：2020-11-30 00:00:00

abstract：:Consumption of poultry contaminated with Campylobacter jejuni is a risk factor for human gastrointestinal disease. The rational development of control strategies for Campylobacter within chickens requires an understanding of the colonization process at the molecular and population levels, both within and between hosts...

journal_title：Applied and environmental microbiology

authors： Coward C,van Diemen PM,Conlan AJ,Gog JR,Stevens MP,Jones MA,Maskell DJ

更新日期：2008-06-01 00:00:00

abstract：:The restriction endonuclease Cac824I has been shown to be a major barrier to electrotransformation of Clostridium acetobutylicum ATCC 824 (L. D. Mermelstein, N. E. Welker, G. N. Bennett, and E. T. Papoutsakis, Bio/Technology 10:190-195, 1992). Methylation by the phi 3T I methyltransferase encoded by Bacillus subtilis ...

journal_title：Applied and environmental microbiology

authors： Mermelstein LD,Papoutsakis ET

更新日期：1993-04-01 00:00:00

abstract：:Several selective media were evaluated for the primary isolation and enumeration of Staphylococcus aureus from halogenated indoor swimming pool waters. Standard plate counts of the viable population and total coliform densities were also determined to ascertain their value as indicator systems. All studies were done w...

journal_title：Applied and environmental microbiology

authors： Alico RK,Dragonjac MF

更新日期：1986-04-01 00:00:00

abstract：:The heat destruction characteristics of Clostridium botulinum spores suspended in tomato juice and phosphate buffer were determined by the survivor curve method with aluminum thermal death time tubes. Two type A strains of C. botulinum and a type B strain were evaluated. Strains A16037 and B15580 were implicated in ou...

journal_title：Applied and environmental microbiology

authors： Odlaug TE,Pflug IJ

更新日期：1977-07-01 00:00:00

abstract：:A methanotroph (strain 68-1), originally isolated from a trichloroethylene (TCE)-contaminated aquifer, was identified as the type I methanotroph Methylomonas methanica on the basis of intracytoplasmic membrane ultrastructure, phospholipid fatty acid profile, and 16S rRNA signature probe hybridization. Strain 68-1 was ...

journal_title：Applied and environmental microbiology

authors： Koh SC,Bowman JP,Sayler GS

更新日期：1993-04-01 00:00:00