New electropositive filter for concentrating enteroviruses and noroviruses from large volumes of water.

Abstract:

:The U.S. Environmental Protection Agency's information collection rule requires the use of 1MDS electropositive filters for concentrating enteric viruses from water, but unfortunately, these filters are not cost-effective for routine viral monitoring. In this study, an inexpensive electropositive cartridge filter, the NanoCeram filter, was evaluated for its ability to concentrate enteroviruses and noroviruses from large volumes of water. Seeded viruses were concentrated using the adsorption-elution procedure. The mean percent retention of seeded polioviruses by NanoCeram filters was 84%. To optimize the elution procedure, six protocols, each comprising two successive elutions with various lengths of filter immersion, were evaluated. The highest virus recovery (77%) was obtained by immersing the filters in beef extract for 1 minute during the first elution and for 15 min during the second elution. The recovery efficiencies of poliovirus, coxsackievirus B5, and echovirus 7 from 100-liter samples of seeded tap water were 54%, 27%, and 32%, respectively. There was no significant difference in virus recovery from tap water with a pH range of 6 to 9.5 and a water flow rate range of 5.5 liters/min to 20 liters/min. Finally, poliovirus and Norwalk virus recoveries by NanoCeram filters were compared to those by 1MDS filters, using tap water and Ohio River water. Poliovirus and Norwalk virus recoveries by NanoCeram filters from tap and river water were similar to or higher than those by the 1MDS filters. These data suggest that NanoCeram filters can be used as an inexpensive alternative to 1MDS filters for routine viral monitoring of water.

journal_name

Appl Environ Microbiol

authors

Karim MR,Rhodes ER,Brinkman N,Wymer L,Fout GS

doi

10.1128/AEM.00922-08

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

2393-9

issue

8

eissn

0099-2240

issn

1098-5336

pii

AEM.00922-08

journal_volume

75

pub_type

杂志文章
  • Semiquantitative Performance and Mechanism Evaluation of Carbon Nanomaterials as Cathode Coatings for Microbial Fouling Reduction.

    abstract::In this study, we examine bacterial attachment and survival on a titanium (Ti) cathode coated with various carbon nanomaterials (CNM): pristine carbon nanotubes (CNT), oxidized carbon nanotubes (O-CNT), oxidized-annealed carbon nanotubes (OA-CNT), carbon black (CB), and reduced graphene oxide (rGO). The carbon nanomat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00582-15

    authors: Zhang Q,Nghiem J,Silverberg GJ,Vecitis CD

    更新日期:2015-07-01 00:00:00

  • Comparative characterization of complete and truncated forms of Lactobacillus amylovorus alpha-amylase and role of the C-terminal direct repeats in raw-starch binding.

    abstract::Two constructs derived from the alpha-amylase gene (amyA) of Lactobacillus amylovorus were expressed in Lactobacillus plantarum, and their expression products were purified, characterized, and compared. These products correspond to the complete (AmyA) and truncated (AmyADelta) forms of alpha-amylase; AmyADelta lacks t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.8.3350-3356.2000

    authors: Rodriguez Sanoja R,Morlon-Guyot J,Jore J,Pintado J,Juge N,Guyot JP

    更新日期:2000-08-01 00:00:00

  • Novel antibiotic-free plasmid selection system based on complementation of host auxotrophy in the NAD de novo synthesis pathway.

    abstract::The use of antibiotic resistance genes in plasmids causes potential biosafety and clinical hazards, such as the possibility of horizontal spread of resistance genes or the rapid emergence of multidrug-resistant pathogens. This paper introduces a novel auxotrophy complementation system that allowed plasmids and host ce...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02462-09

    authors: Dong WR,Xiang LX,Shao JZ

    更新日期:2010-04-01 00:00:00

  • Genetic characterization of plasmid-associated benzalkonium chloride resistance determinants in a Listeria monocytogenes strain from the 1998-1999 outbreak.

    abstract::Quaternary ammonium compounds such as benzalkonium chloride (BC) are widely used as disinfectants in both food processing and medical environments. BC-resistant strains of Listeria monocytogenes have been implicated in multistate outbreaks of listeriosis and have been frequently isolated from food processing plants. H...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02056-10

    authors: Elhanafi D,Dutta V,Kathariou S

    更新日期:2010-12-01 00:00:00

  • Cloning of a phenol oxidase gene from Acremonium murorum and its expression in Aspergillus awamori.

    abstract::Fungal multicopper oxidases have many potential industrial applications, since they perform reactions under mild conditions. We isolated a phenol oxidase from the fungus Acremonium murorum var. murorum that was capable of decolorizing plant chromophores (such as anthocyanins). This enzyme is of interest in laundry-cle...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.6.2610-2616.2001

    authors: Gouka RJ,van der Heiden M,Swarthoff T,Verrips CT

    更新日期:2001-06-01 00:00:00

  • Effects of bacterial antibiotic production on rhizosphere microbial communities from a culture-independent perspective

    abstract::The effects of antibiotic production on rhizosphere microbial communities of field-grown Phaseolus vulgaris were assessed by using ribosomal intergenic spacer analysis. Inoculum strains of Rhizobium etli CE3 differing only in trifolitoxin production were used. Trifolitoxin production dramatically reduced the diversity...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.12.5020-5022.1998

    authors: Robleto EA,Borneman J,Triplett EW

    更新日期:1998-12-01 00:00:00

  • Purification and Characterization of an Autolysin from Clostridium acetobutylicum.

    abstract::A proteinaceous substance with antibiotic-like activity, resembling that of a bacteriocin, was isolated from an industrial-scale acetone-butanol fermentation of Clostridium acetobutylicum. The substance, purified by acetone precipitation, diethylaminoethyl cellulose chromatography, and polyacrylamide gel electrophores...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.2.371-374.1981

    authors: Webster JR,Reid SJ,Jones DT,Woods DR

    更新日期:1981-02-01 00:00:00

  • Relationship of species-specific filament levels to filamentous bulking in activated sludge.

    abstract::To examine the relationship between activated-sludge bulking and levels of specific filamentous bacteria, we developed a statistics-based quantification method for estimating the biomass levels of specific filaments using 16S rRNA-targeted fluorescent in situ hybridization (FISH) probes. The results of quantitative FI...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.4.2420-2428.2004

    authors: Liao J,Lou I,de los Reyes FL 3rd

    更新日期:2004-04-01 00:00:00

  • Volatile Compounds Associated with Spoilage of Vacuum-Packaged Sliced Luncheon Meat by Brochothrix thermosphacta.

    abstract::By using gas chromatography-mass spectrometry, seven volatile compounds were identified in vacuum-packaged sliced corned beef spoiled by Brochothrix thermosphacta under aerobic conditions. Acetoin and diacetyl appeared to be of major sensory significance. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.3.816-818.1981

    authors: Stanley G,Shaw KJ,Egan AF

    更新日期:1981-03-01 00:00:00

  • Active-Site Engineering of ω-Transaminase for Production of Unnatural Amino Acids Carrying a Side Chain Bulkier than an Ethyl Substituent.

    abstract::ω-Transaminase (ω-TA) is a promising enzyme for use in the production of unnatural amino acids from keto acids using cheap amino donors such as isopropylamine. The small substrate-binding pocket of most ω-TAs permits entry of substituents no larger than an ethyl group, which presents a significant challenge to the pre...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01533-15

    authors: Han SW,Park ES,Dong JY,Shin JS

    更新日期:2015-10-01 00:00:00

  • Cytopathology of pathogenic and nonpathogenic Naegleria species for cultured rat neuroblastoma cells.

    abstract::The cytopathology for rat neuroblastoma cells (B-103) and the pathogenicity for B6C3F1 mice of four species of Naegleria have been compared. Both live amoebae and cell-free extracts of N. australiensis, N. fowleri, N. gruberi, and N. lovaniensis added to 51Cr-labeled B-103 cells caused release of radiolabel. All four ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.5.1133-1137.1986

    authors: Marciano-Cabral FM,Fulford DE

    更新日期:1986-05-01 00:00:00

  • Microflora of partially processed lettuce.

    abstract::Bacteria, yeasts, and molds isolated from partially processed iceberg lettuce were taxonomically classified. The majority of bacterial isolates were gram-negative rods. Pseudomonas, Erwinia, and Serratia species were commonly found. Yeasts most frequently isolated from lettuce included members of the genera Candida, C...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.12.3851-3854.1990

    authors: Magnuson JA,King AD Jr,Török T

    更新日期:1990-12-01 00:00:00

  • Cultivation-independent examination of horizontal transfer and host range of an IncP-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere.

    abstract::The host range and transfer frequency of an IncP-1 plasmid (pKJK10) among indigenous bacteria in the barley rhizosphere was investigated. A new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16S rRNA gene classification was used. Indigenous transconjug...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00013-06

    authors: Musovic S,Oregaard G,Kroer N,Sørensen SJ

    更新日期:2006-10-01 00:00:00

  • Disentangling the Taxonomy of Rickettsiales and Description of Two Novel Symbionts ("Candidatus Bealeia paramacronuclearis" and "Candidatus Fokinia cryptica") Sharing the Cytoplasm of the Ciliate Protist Paramecium biaurelia.

    abstract::In the past 10 years, the number of endosymbionts described within the bacterial order Rickettsiales has constantly grown. Since 2006, 18 novel Rickettsiales genera inhabiting protists, such as ciliates and amoebae, have been described. In this work, we characterize two novel bacterial endosymbionts from Paramecium co...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02284-16

    authors: Szokoli F,Castelli M,Sabaneyeva E,Schrallhammer M,Krenek S,Doak TG,Berendonk TU,Petroni G

    更新日期:2016-11-21 00:00:00

  • Carbon allocation in wild-type and Glc+ Rhodobacter sphaeroides under photoheterotrophic conditions.

    abstract::The photosynthetic bacterium Rhodobacter sphaeroides is capable of producing H2 via nitrogenase when grown photoheterotrophically in the absence of N2. By using 14C-labeled malate, it was found that greater than 95% of this substrate was catabolized completely to CO2 during H2 production. About 60% of this catabolism ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.11.2737-2741.1988

    authors: Macler BA,Bassham JA

    更新日期:1988-11-01 00:00:00

  • Carrot Juice Fermentations as Man-Made Microbial Ecosystems Dominated by Lactic Acid Bacteria.

    abstract::Spontaneous vegetable fermentations, with their rich flavors and postulated health benefits, are regaining popularity. However, their microbiology is still poorly understood, therefore raising concerns about food safety. In addition, such spontaneous fermentations form interesting cases of man-made microbial ecosystem...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00134-18

    authors: Wuyts S,Van Beeck W,Oerlemans EFM,Wittouck S,Claes IJJ,De Boeck I,Weckx S,Lievens B,De Vuyst L,Lebeer S

    更新日期:2018-05-31 00:00:00

  • Development and testing of a bacterial biosensor for toluene-based environmental contaminants.

    abstract::A bacterial biosensor for benzene, toluene, and similar compounds has been constructed, characterized, and field tested on contaminated water and soil. The biosensor is based on a plasmid incorporating the transcriptional activator xylR from the TOL plasmid of Pseudomonas putida mt-2. The XylR protein binds a subset o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.3.1006-1012.1998

    authors: Willardson BM,Wilkins JF,Rand TA,Schupp JM,Hill KK,Keim P,Jackson PJ

    更新日期:1998-03-01 00:00:00

  • Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa.

    abstract::Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is str...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04028-12

    authors: Giebler J,Wick LY,Schloter M,Harms H,Chatzinotas A

    更新日期:2013-05-01 00:00:00

  • Microbial community shifts reflect losses of native soil carbon with pyrogenic and fresh organic matter additions and are greatest in low-carbon soils.

    abstract::Soil organic carbon (SOC) plays an important role in regulating global climate change, carbon and nutrient cycling in soils, and soil moisture. Organic matter (OM) additions to soils can affect the rate at which SOC is mineralized by microbes, with potentially important effects on SOC stocks. Understanding how pyrogen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02555-20

    authors: Whitman T,DeCiucies S,Hanley K,Enders A,Woolet J,Lehmann J

    更新日期:2021-01-29 00:00:00

  • Isolation and characterization of a freeze-tolerant diploid derivative of an industrial baker's yeast strain and its use in frozen doughs.

    abstract::The routine production and storage of frozen doughs are still problematic. Although commercial baker's yeast is highly resistant to environmental stress conditions, it rapidly loses stress resistance during dough preparation due to the initiation of fermentation. As a result, the yeast loses gassing power significantl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.10.4780-4787.2002

    authors: Teunissen A,Dumortier F,Gorwa MF,Bauer J,Tanghe A,Loïez A,Smet P,Van Dijck P,Thevelein JM

    更新日期:2002-10-01 00:00:00

  • Blame It on the Metabolite: 3,5-Dichloroaniline Rather than the Parent Compound Is Responsible for the Decreasing Diversity and Function of Soil Microorganisms.

    abstract::Pesticides are key stressors of soil microorganisms with reciprocal effects on ecosystem functioning. These effects have been mainly attributed to the parent compounds, while the impact of their transformation products (TPs) has been largely overlooked. We assessed in a meadow soil (soil A) the transformation of iprod...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01536-18

    authors: Vasileiadis S,Puglisi E,Papadopoulou ES,Pertile G,Suciu N,Pappolla RA,Tourna M,Karas PA,Papadimitriou F,Kasiotakis A,Ipsilanti N,Ferrarini A,Sułowicz S,Fornasier F,Menkissoglu-Spiroudi U,Nicol GW,Trevisan M,Karpouzas DG

    更新日期:2018-10-30 00:00:00

  • PbaR, an IclR family transcriptional activator for the regulation of the 3-phenoxybenzoate 1',2'-dioxygenase gene cluster in Sphingobium wenxiniae JZ-1T.

    abstract::The 3-phenoxybenzoate (3-PBA) 1',2'-dioxygenase gene cluster (pbaA1A2B cluster), which is responsible for catalyzing 3-phenoxybenzoate to 3-hydroxybenzoate and catechol, is inducibly expressed in Sphingobium wenxiniae strain JZ-1(T) by its substrate 3-PBA. In this study, we identified a transcriptional activator of th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02122-15

    authors: Cheng M,Chen K,Guo S,Huang X,He J,Li S,Jiang J

    更新日期:2015-12-01 00:00:00

  • First evidence of lysogeny in Propionibacterium freudenreichii subsp. shermanii.

    abstract::Dairy propionic acid bacteria, particularly the species Propionibacterium freudenreichii, play a major role in the ripening of Swiss type cheese. Isometric and filamentous bacteriophages infecting P. freudenreichii have previously been isolated from cheese. In order to determine the origin of these bacteriophages, lys...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.1.231-238.2001

    authors: Hervé C,Coste A,Rouault A,Fraslin JM,Gautier M

    更新日期:2001-01-01 00:00:00

  • Distribution of Vibrio vulnificus phage in oyster tissues and other estuarine habitats.

    abstract::Phages lytic to Vibrio vulnificus were found in estuarine waters, sediments, plankton, crustacea, molluscan shellfish, and the intestines of finfish of the U.S. Gulf Coast, but no apparent relationship between densities of V. vulnificus and its phages was observed. Phage diversity and abundance in molluscan shellfish ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.6.2464-2467.1997

    authors: DePaola A,McLeroy S,McManus G

    更新日期:1997-06-01 00:00:00

  • Spoilage of chicken skin at 2 degrees C: electron microscopic study.

    abstract::Microscopic techniques were used in conjunction with normal microbiological procedures to examine the development of the spoilage microflora on the skin of chicken carcasses held at 2 degrees C. Pigmented and nonpigmented psychrotrophic pseudomonads were the major spoilage bacteria isolated at all stages of storage ex...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.2.492-503.1981

    authors: Thomas CJ,McMeekin TA

    更新日期:1981-02-01 00:00:00

  • Multifactorial effects of ambient temperature, precipitation, farm management, and environmental factors determine the level of generic Escherichia coli contamination on preharvested spinach.

    abstract::A repeated cross-sectional study was conducted to identify farm management, environment, weather, and landscape factors that predict the count of generic Escherichia coli on spinach at the preharvest level. E. coli was enumerated for 955 spinach samples collected on 12 farms in Texas and Colorado between 2010 and 2012...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03793-14

    authors: Park S,Navratil S,Gregory A,Bauer A,Srinath I,Szonyi B,Nightingale K,Anciso J,Jun M,Han D,Lawhon S,Ivanek R

    更新日期:2015-04-01 00:00:00

  • Prevalence and relatedness of Escherichia coli O157:H7 strains in the feces and on the hides and carcasses of U.S. meat goats at slaughter.

    abstract::We determined the prevalences of Escherichia coli O157:H7 in feces, hide, and carcasses of meat goats at a U.S. processing plant. Prevalences were 11.1%, 2.7%, and 2.7%, respectively. Sixteen pulsed-field gel electrophoresis (PFGE) subtypes were identified among 49 E. coli O157:H7 isolates, some of which were present ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00772-13

    authors: Jacob ME,Foster DM,Rogers AT,Balcomb CC,Sanderson MW

    更新日期:2013-07-01 00:00:00

  • Magnetospirillum bellicus sp. nov., a novel dissimilatory perchlorate-reducing alphaproteobacterium isolated from a bioelectrical reactor.

    abstract::Previously isolated dissimilatory perchlorate-reducing bacteria (DPRB) have been primarily affiliated with the Betaproteobacteria. Enrichments from the cathodic chamber of a bioelectrical reactor (BER) inoculated from creek water in Berkeley, CA, yielded a novel organism most closely related to a previously described ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00015-10

    authors: Thrash JC,Ahmadi S,Torok T,Coates JD

    更新日期:2010-07-01 00:00:00

  • Ribotyping to differentiate Fusobacterium necrophorum subsp. necrophorum and F. necrophorum subsp. funduliforme isolated from bovine ruminal contents and liver abscesses.

    abstract::Differences in biological activities (hemagglutination, hemolytic, leukotoxic, and virulence) and ribotypes between the two subspecies of Fusobacterium necrophorum of bovine ruminal and liver abscess origins were investigated. Hemagglutination activity was present in all hepatic, but only some ruminal, strains of Fuso...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.2.469-472.1996

    authors: Okwumabua O,Tan Z,Staats J,Oberst RD,Chengappa MM,Nagaraja TG

    更新日期:1996-02-01 00:00:00

  • Production of ochratoxin A in barley by Aspergillus ochraceus and Penicillium viridicatum: effect of fungal growth, time, temperature, and inoculum size.

    abstract::Moistened barley was inoculated with 1.4 x 10(3) and 1.4 x 10(5) spores, respectively, from ochratoxin A-producing strains of Aspergillus ochraceus and Penicillium varidicatum. To estimate fungal tissue in the barley, the amount of glucosamine was followed for 28 days at 10 and 25 degrees C. Ochratoxin A was also foll...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.5.1205-1207.1982

    authors: Häggblom P

    更新日期:1982-05-01 00:00:00