Glyphosate resistance as a novel select-agent-compliant, non-antibiotic-selectable marker in chromosomal mutagenesis of the essential genes asd and dapB of Burkholderia pseudomallei.

abstract：:Despite the increasing knowledge of Synechococcus spp. and their co-occurring cyanophages in oceanic and coastal water, little is known about their abundance, distribution, and interactions in the Chesapeake Bay estuarine ecosystem. A 5-year interannual survey shows that Synechococcus spp. and their phages are persist...

journal_title：Applied and environmental microbiology

authors： Wang K,Wommack KE,Chen F

更新日期：2011-11-01 00:00:00

abstract：:We describe the stabilization by pressure of enzymes, including a hydrogenase from Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen. This is the first published report of proteins from thermophiles being stabilized by pressure. Inactivation studies of partially purified hydrogenases from an extr...

journal_title：Applied and environmental microbiology

authors： Hei DJ,Clark DS

更新日期：1994-03-01 00:00:00

abstract：:An NAD(P)H-nicotine blue (quinone) oxidoreductase was discovered as a member of the nicotine catabolic pathway of Arthrobacter nicotinovorans. Transcriptional analysis and electromobility shift assays showed that the enzyme gene was expressed in a nicotine-dependent manner under the control of the transcriptional acti...

journal_title：Applied and environmental microbiology

authors： Mihasan M,Chiribau CB,Friedrich T,Artenie V,Brandsch R

更新日期：2007-04-01 00:00:00

abstract：:Campylobacter jejuni and Campylobacter coli are naturally competent, but limited information exists on the impact of environmental conditions on transformation. In this study, we investigated the impact of temperature and microaerobic versus aerobic atmosphere on transformation of C. coli to erythromycin and nalidixic...

journal_title：Applied and environmental microbiology

authors： Kim JS,Kim JW,Kathariou S

更新日期：2008-10-01 00:00:00

abstract：:We modeled mold growth on a solid culture medium at various temperatures and NaCl concentrations by using five common food spoilage molds (Penicillium roqueforti, Trichoderma harzianum, Paecilomyces variotii, Aspergillus niger, and Emericella nidulans). For the description of the growth rate (expressed as the increase...

journal_title：Applied and environmental microbiology

authors： Cuppers HG,Oomes S,Brul S

更新日期：1997-10-01 00:00:00

abstract：:Parasitic chytrid fungi can inflict significant mortality on cyanobacteria but frequently fail to keep cyanobacterial dominance and bloom formation in check. Our study tested whether oligopeptide production, a common feature in many cyanobacteria, can be a defensive mechanism against chytrid parasitism. The study empl...

journal_title：Applied and environmental microbiology

authors： Rohrlack T,Christiansen G,Kurmayer R

更新日期：2013-04-01 00:00:00

abstract：UNLABELLED:Mechanistic studies of many processes in Agrobacterium tumefaciens have been hampered by a lack of genetic tools for characterization of essential genes. In this study, we used a Tn7-based method for inducible control of transcription from an engineered site on the chromosome. We demonstrate that this method...

journal_title：Applied and environmental microbiology

authors： Figueroa-Cuilan W,Daniel JJ,Howell M,Sulaiman A,Brown PJ

更新日期：2016-07-29 00:00:00

abstract：:Pigment production by the fungus Monascus sp. was studied to determine why Monascus sp. provides more pigment in solid culture than in submerged culture. Adding a sterilized nonionic polymeric adsorbent resin directly to the growing submerged culture did not enhance the pigment production, thus indicating that pigment...

journal_title：Applied and environmental microbiology

authors： Evans PJ,Wang HY

更新日期：1984-06-01 00:00:00

abstract：:Alteromonas is a widely distributed genus of marine Gammaproteobacteria, with representatives shown to be key players in diverse processes, including biogeochemical cycling and biofouling of marine substrata. While Alteromonas spp. are early colonizers of copper-based antifouling paints on marine vessels, their mechan...

journal_title：Applied and environmental microbiology

authors： Cusick KD,Polson SW,Duran G,Hill RT

更新日期：2020-01-21 00:00:00

abstract：:This study investigated the differences in microbial community abundance, composition and diversity throughout the depth profiles in soils collected from corn and soybean fields in lowa, USA using 16S rRNA amplicon sequencing. The results revealed decreased richness and diversity in microbial communities at increasing...

journal_title：Applied and environmental microbiology

authors： Hao J,Chai YN,Lopes LD,Ordóñez RA,Wright EE,Archontoulis S,Schachtman DP

更新日期：2020-12-11 00:00:00

abstract：:Erwinia amylovora bacteriophages (phages) belonging to the Myoviridae and Podoviridae families demonstrated a preference for either high-exopolysaccharide-producing (HEP) or low-exopolysaccharide-producing (LEP) bacterial hosts when grown on artificial medium without or with sugar supplementation. Myoviridae phages pr...

journal_title：Applied and environmental microbiology

authors： Roach DR,Sjaarda DR,Castle AJ,Svircev AM

更新日期：2013-05-01 00:00:00

abstract：:In a previous study by our group, CH4 oxidation and N2 fixation were simultaneously activated in the roots of wild-type rice plants in a paddy field with no N input; both processes are likely controlled by a rice gene for microbial symbiosis. The present study examined which microorganisms in rice roots were responsib...

journal_title：Applied and environmental microbiology

authors： Bao Z,Okubo T,Kubota K,Kasahara Y,Tsurumaru H,Anda M,Ikeda S,Minamisawa K

更新日期：2014-08-01 00:00:00

abstract：:A plasmid marker rescue system based on restoration of the nptII gene was established in Streptococcus gordonii to study the transfer of bacterial and transgenic plant DNA by transformation. In vitro studies revealed that the marker rescue efficiency depends on the type of donor DNA. Plasmid and chromosomal DNA of bac...

journal_title：Applied and environmental microbiology

authors： Kharazmi M,Sczesny S,Blaut M,Hammes WP,Hertel C

更新日期：2003-10-01 00:00:00

abstract：:Bacterial strains were isolated on the basis of their ability to proliferate in a minimal medium containing one of a series of lignin-related compounds as the sole carbon and energy source. These included the aromatic monomers guaiacol, vanillic and coumaric acids, a dimer and a trimer possessing the arylglycerol-beta...

journal_title：Applied and environmental microbiology

authors： Gonzalez B,Merino A,Almeida M,Vicña R

更新日期：1986-12-01 00:00:00

abstract：:The abundance of nifH, nirS, and nirK gene fragments involved in nitrogen (N) fixation and denitrification in thinned second-growth Douglas-fir (Pseudotsuga menziesii subsp. menziesii [Mirb.] Franco) forest soil was investigated by using quantitative real-time PCR. Prokaryotic N cycling is an important aspect of N ava...

journal_title：Applied and environmental microbiology

authors： Levy-Booth DJ,Winder RS

更新日期：2010-11-01 00:00:00

abstract：:Opaque and translucent morphotypes of a TnphoA-containing strain of Vibrio vulnificus were fed to oysters, which were subsequently stored at temperatures ranging from 0.5 to 22 degrees C for 10 days. Samples of oysters were homogenized and plated at intervals to determine the cell density of V. vulnificus and total ae...

journal_title：Applied and environmental microbiology

authors： Murphy SK,Oliver JD

更新日期：1992-09-01 00:00:00

abstract：:Oysters collected in late winter, when they were free of Vibrio vulnificus, were exposed in the organism in the laboratory. The oysters effectively concentrated the bacteria from seawater, but when the inoculum was removed, the bacteria were rapidly cleared from the oyster tissues. These results suggest that V. vulnif...

journal_title：Applied and environmental microbiology

authors： Kelly MT,Dinuzzo A

更新日期：1985-12-01 00:00:00

abstract：:We have shown that the plant pathogen Alternaria brassicicola exhibited very high susceptibility to ambruticin VS4 and to a lesser extent to the phenylpyrrole fungicide fludioxonil. These compounds are both derived from natural bacterial metabolites with antifungal properties and are thought to exert their toxicity by...

journal_title：Applied and environmental microbiology

authors： Dongo A,Bataillé-Simoneau N,Campion C,Guillemette T,Hamon B,Iacomi-Vasilescu B,Katz L,Simoneau P

更新日期：2009-01-01 00:00:00

abstract：:Streptococcus mutans is a key cariogenic bacterium responsible for the initiation of tooth decay. Biofilm formation is a crucial virulence property. We discovered a putative glycosyltransferase, SMU_833, in S. mutans capable of modulating dynamic interactions between two key biofilm matrix components, glucan and extra...

journal_title：Applied and environmental microbiology

authors： Rainey K,Michalek SM,Wen ZT,Wu H

更新日期：2019-02-20 00:00:00

abstract：:The effect of addition of purified nisin Z in liposomes to cheese milk and of in situ production of nisin Z by Lactococcus lactis subsp. lactis biovar diacetylactis UL719 in the mixed starter on the inhibition of Listeria innocua in cheddar cheese was evaluated during 6 months of ripening. A cheese mixed starter cultu...

journal_title：Applied and environmental microbiology

authors： Benech RO,Kheadr EE,Laridi R,Lacroix C,Fliss I

更新日期：2002-08-01 00:00:00

abstract：:Stromatolites, organosedimentary structures formed by microbial activity, are found throughout the geological record and are important markers of biological history. More conspicuous in the past, stromatolites occur today in a few shallow marine environments, including Hamelin Pool in Shark Bay, Western Australia. Ham...

journal_title：Applied and environmental microbiology

authors： Papineau D,Walker JJ,Mojzsis SJ,Pace NR

更新日期：2005-08-01 00:00:00

abstract：:The composition of the rhizosphere microbiome is a result of interactions between plant roots, soil, and environmental conditions. The impact of genetic variation in plant species on the composition of the root-associated microbiota remains poorly understood. This study assessed the abundances and structures of nitrog...

journal_title：Applied and environmental microbiology

authors： Cotta SR,Dias AC,Marriel IE,Andreote FD,Seldin L,van Elsas JD

更新日期：2014-10-01 00:00:00

abstract：:Clostridium acetobutylicum is not able to grow on glycerol as the sole carbon source since it cannot reoxidize the excess of NADH generated by glycerol catabolism. Nevertheless, when the pSPD5 plasmid, carrying the NADH-consuming 1,3-propanediol pathway from C. butyricum VPI 3266, was introduced into C. acetobutylicum...

journal_title：Applied and environmental microbiology

authors： González-Pajuelo M,Meynial-Salles I,Mendes F,Soucaille P,Vasconcelos I

更新日期：2006-01-01 00:00:00

abstract：:Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about their survival in intact cow pats depo...

journal_title：Applied and environmental microbiology

authors： Oladeinde A,Bohrmann T,Wong K,Purucker ST,Bradshaw K,Brown R,Snyder B,Molina M

更新日期：2014-01-01 00:00:00

abstract：:The genus Aeromonas includes some species that have now been identified as human pathogens of significant medical importance. We investigated the ability of 13 selected Aeromonas strains belonging to nine species isolated from clinical cases (n = 5), environmental waters (n = 5), and fish (n = 3) to adhere to and tran...

journal_title：Applied and environmental microbiology

authors： Hatje E,Neuman C,Katouli M

更新日期：2014-01-01 00:00:00

abstract：:The adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and negatively polarized thin films of gold was studied in situ by contrast microscopy using a thin-film electrochemical flow cell. The influence of the electrochemical potential was evaluated at two different ionic strengths (0.01 and 0.1 M NaCl; pH...

journal_title：Applied and environmental microbiology

authors： Busalmen JP,de Sánchez SR

更新日期：2001-07-01 00:00:00

abstract：:Nineteen Tn5-induced mutants of Rhizobium fredii HH303 defective in acidic exopolysaccharide synthesis were isolated by screening for lack of Calcofluor fluorescence. They were grouped by complementation analysis by using Rhizobium meliloti cosmids carrying exo genes. All of the 19 mutants were symbiotically effective...

journal_title：Applied and environmental microbiology

authors： Kim CH,Tully RE,Keister DL

更新日期：1989-07-01 00:00:00

abstract：:Rhizomucor miehei lipase (RML), as a kind of eukaryotic protein catalyst, plays an important role in the food, organic chemical, and biofuel industries. However, RML retains its catalytic activity below 50°C, which limits its industrial applications at higher temperatures. Soluble expression of this eukaryotic protein...

journal_title：Applied and environmental microbiology

authors： Li G,Fang X,Su F,Chen Y,Xu L,Yan Y

更新日期：2018-01-02 00:00:00

abstract：:The degradation of the herbicides EPTC (S-ethyl dipropylthiocarbamate) and atrazine (2-chloro-4-ethyl-amino-6-isopropylamino-1,3,5-triazine) is associated with an indigenous plasmid in Rhodococcus sp. strain TE1. Plasmid DNA libraries of Rhodococcus sp. strain TE1 were constructed in a Rhodococcus-Escherichia coli shu...

journal_title：Applied and environmental microbiology

authors： Shao ZQ,Behki R

更新日期：1995-05-01 00:00:00

abstract：:Two strains of Penicillium digitatum and one strain of Penicillium italicum were exposed to various levels of sorbic acid and potassium sorbate, and the MICs were determined. Selected strains of the molds were then repeatedly exposed to subinhibitory levels of the compounds to determine whether increased tolerance mig...

journal_title：Applied and environmental microbiology

authors： Schroeder LL,Bullerman LB

更新日期：1985-10-01 00:00:00