Mini-Tn7 Insertion in an Artificial attTn7 Site Enables Depletion of the Essential Master Regulator CtrA in the Phytopathogen Agrobacterium tumefaciens.

abstract：:The so-called Fe/Mn-oxidizing bacteria have long been recognized for their potential to form extracellular iron hydroxide or manganese oxide structures in aquatic environments. Bacterial species belonging to the genus Gallionella, one type of such bacteria, oxidize iron and produce uniquely twisted extracellular stalk...

journal_title：Applied and environmental microbiology

authors： Suzuki T,Hashimoto H,Matsumoto N,Furutani M,Kunoh H,Takada J

更新日期：2011-05-01 00:00:00

abstract：:A Coxiella-type microbe occurs at 100% frequency in all Amblyomma americanum ticks thus far tested. Using laboratory-reared ticks free of other microbes, we identified the Amblyomma-associated Coxiella microbe in several types of tissue and at various stages of the life cycle of A. americanum by 16S rRNA gene sequenci...

journal_title：Applied and environmental microbiology

authors： Klyachko O,Stein BD,Grindle N,Clay K,Fuqua C

更新日期：2007-10-01 00:00:00

abstract：:Xanthobacter flavus 14p1 was isolated from sludge of the river Mulde by selective enrichment with 1,4-dichlorobenzene as the sole source of carbon and energy. The bacterium did not use other aromatic or chloroaromatic compounds as growth substrates. During growth on 1,4-dichlorobenzene, stoichiometric amounts of chlor...

journal_title：Applied and environmental microbiology

authors： Spiess E,Sommer C,Görisch H

更新日期：1995-11-01 00:00:00

abstract：:Aldehydes are a class of chemicals with many industrial uses. Several aldehydes are responsible for flavors and fragrances present in plants, but aldehydes are not known to accumulate in most natural microorganisms. In many cases, microbial production of aldehydes presents an attractive alternative to extraction from ...

journal_title：Applied and environmental microbiology

authors： Kunjapur AM,Prather KL

更新日期：2015-03-01 00:00:00

abstract：:A marine bacterial strain that decomposes the cell walls of some seaweeds, including a Laminaria sp. and Undaria pinnatifida, has been isolated from seawater. This strain has been classified to the genus Vibrio. One of the enzymes which the bacteria secreted into the culture medium was isolated and purified 45-fold fr...

journal_title：Applied and environmental microbiology

authors： Sugano Y,Terada I,Arita M,Noma M,Matsumoto T

更新日期：1993-05-01 00:00:00

abstract：:The induction of hydroxyl radical (OH) production via quinone redox cycling in white-rot fungi was investigated to improve pollutant degradation. In particular, we examined the influence of 4-methoxybenzaldehyde (anisaldehyde), Mn(2+), and oxalate on Pleurotus eryngii OH generation. Our standard quinone redox cycling ...

journal_title：Applied and environmental microbiology

authors： Gómez-Toribio V,García-Martín AB,Martínez MJ,Martínez AT,Guillén F

更新日期：2009-06-01 00:00:00

abstract：:NAD-linked alcohol dehydrogenase activity was detected in cell-free crude extracts from various propane-grown bacteria. Two NAD-linked alcohol dehydrogenases, one which preferred primary alcohols (alcohol dehydrogenase I) and another which preferred secondary alcohols (alcohol dehydrogenase II), were found in propane-...

journal_title：Applied and environmental microbiology

authors： Hou CT,Patel RN,Laskin AI,Barist I,Barnabe N

更新日期：1983-07-01 00:00:00

abstract：:Twenty-four Escherichia coli strains producing standard colicins were evaluated for inhibitory activity against 27 diarrheagenic E. coli strains of serotypes O15:H-, O26:(H11, H-), and O111:(H8, H11, H-), including O157:H7, representing diarrheagenic E. coli clones, 3, 4, 8, 9, and 10. Overlay techniques were used to ...

journal_title：Applied and environmental microbiology

authors： Murinda SE,Roberts RF,Wilson RA

更新日期：1996-09-01 00:00:00

abstract：:Halophenols such as 2,4-dibromophenol (DBP) occur naturally in some marine sediments, as a consequence of various animal and algal activities. In an earlier study, DBP was observed in the burrow microenvironment of the hemichordate Saccoglossus kowalewskii. At the concentrations found in the burrow lining, aerobic res...

journal_title：Applied and environmental microbiology

authors： King GM

更新日期：1988-12-01 00:00:00

abstract：:Altogether, 100 strains of Listeria monocytogenes serovar 1/2a isolated from humans, animals, food, and the environment were typed by a combination of PCR and restriction enzyme analysis (REA). A PCR product of 2,916 bp, containing the downstream end of the gene inlA (955 bp), the space between inlA and inlB (85 bp), ...

journal_title：Applied and environmental microbiology

authors： Unnerstad H,Nilsson I,Ericsson H,Danielsson-Tham ML,Bille J,Bannerman E,Tham W

更新日期：1999-05-01 00:00:00

abstract：:Escherichia coli O157:H7 has caused serious outbreaks of food-borne illness via transmission in a variety of food vehicles, including unpasteurized apple juice, dried salami, and spinach. To understand how this pathogen responds to the multiple stresses of the food environment, we compared global transcription pattern...

journal_title：Applied and environmental microbiology

authors： Bergholz TM,Vanaja SK,Whittam TS

更新日期：2009-06-01 00:00:00

abstract：:A highly efficient, rapid, and reliable PCR-based method for distinguishing Lactococcus lactis subspecies (L. lactis subsp. lactis and L. lactis subsp. cremoris) is described. Primers complementary to positions in the glutamate decarboxylase gene have been constructed. PCR analysis with extracted DNA or with cells of ...

journal_title：Applied and environmental microbiology

authors： Nomura M,Kobayashi M,Okamoto T

更新日期：2002-05-01 00:00:00

abstract：:Oxidative stress causes transient actin cytoskeleton depolarization and also provokes vacuole fragmentation in wild-type cells. Under conditions of oxidative stress induced by hydrogen peroxide, the Slt2 protein is required to repolarize the actin cytoskeleton and to promote vacuole fusion. In this study, we show that...

journal_title：Applied and environmental microbiology

authors： Pujol-Carrion N,Petkova MI,Serrano L,de la Torre-Ruiz MA

更新日期：2013-10-01 00:00:00

abstract：:This study concerns the exploitation of the lytic enzyme enterolysin A (EntL), produced by Enterococcus faecalis strain DPC5280, to elicit the controlled autolysis of starter lactococci. EntL, a cell wall metalloendopeptidase secreted by some E. faecalis strains, can kill a wide range of gram-positive bacteria, includ...

journal_title：Applied and environmental microbiology

authors： Hickey RM,Ross RP,Hill C

更新日期：2004-03-01 00:00:00

abstract：:A Rhizobium strain isolated from stem nodules of the legume Sesbania rostrata was shown to grow on atmospheric nitrogen (N(2)) as the sole nitrogen source. Non-N(2)-fixing mutants isolated directly on agar plates formed nodules that did not fix N(2) when inoculated into the host plant. ...

journal_title：Applied and environmental microbiology

authors： Dreyfus BL,Elmerich C,Dommergues YR

更新日期：1983-02-01 00:00:00

abstract：:Increased listerial barotolerance at elevated osmolarity is attributed, in part, to the presence of accumulated betaine and L-carnitine. The percentage of listerial survival following exposure to 400 MPa for 5 min increased from 0.008 to 0.02% with added L-carnitine (5 mM) and to 0.05% with added betaine (5 mM). Furth...

journal_title：Applied and environmental microbiology

authors： Smiddy M,Sleator RD,Patterson MF,Hill C,Kelly AL

更新日期：2004-12-01 00:00:00

abstract：:The activities of two enzymes, a 168-kDa protein and a 40-kDa protein, OmtA, purified from the filamentous fungus Aspergillus parasiticus were reported to convert the aflatoxin pathway intermediate sterigmatocystin to O-methylsterigmatocystin in vitro. Our initial goal was to determine if OmtA is necessary and suffici...

journal_title：Applied and environmental microbiology

authors： Lee LW,Chiou CH,Linz JE

更新日期：2002-11-01 00:00:00

abstract：:Ergosterol, a fungus-specific sterol enriched in cell plasma membranes, is an effective antifungal drug target. However, current knowledge of the ergosterol biosynthesis process in the saprophytic human fungal pathogen Aspergillus fumigatus remains limited. In this study, we found that two endoplasmic reticulum-locali...

journal_title：Applied and environmental microbiology

authors： Long N,Xu X,Zeng Q,Sang H,Lu L

更新日期：2017-02-01 00:00:00

abstract：:Unidentified lactic acid bacterium (LAB) isolates which had mainly been detected in spoiled, marinated, modified atmosphere packaged (MAP) broiler meat products during two previous studies, were identified and analyzed for their phenotypic properties and the capability to produce biogenic amines. To establish the taxo...

journal_title：Applied and environmental microbiology

authors： Koort J,Murros A,Coenye T,Eerola S,Vandamme P,Sukura A,Björkroth J

更新日期：2005-08-01 00:00:00

abstract：:Metabolic engineering and multisubunit protein production necessitate the expression of multiple genes at coordinated levels. In bacteria, genes for multisubunit proteins or metabolic pathways are often expressed in operons under the control of a single promoter; expression of the genes is coordinated by varying trans...

journal_title：Applied and environmental microbiology

authors： Smolke CD,Carrier TA,Keasling JD

更新日期：2000-12-01 00:00:00

abstract：:Two isolates of enterohemorrhagic Escherichia coli (EHEC) O104:H4 were isolated in France in 2004 and 2009. Both were characterized and compared to the strain which caused the German outbreak in 2011 and to other O104:H4 strains. This suggests that different O104:H4 EHEC strains were present several years prior to the...

journal_title：Applied and environmental microbiology

authors： Monecke S,Mariani-Kurkdjian P,Bingen E,Weill FX,Balière C,Slickers P,Ehricht R

更新日期：2011-12-01 00:00:00

abstract：:We developed a novel quantitative real-time PCR (Q-PCR) method for the soil actinomycete Rhodococcus equi, an important horse pathogen and emerging human pathogen. Species-specific quantification was achieved by targeting the chromosomal monocopy gene choE, universally conserved in R. equi. The choE Q-PCR included an ...

journal_title：Applied and environmental microbiology

authors： Rodríguez-Lázaro D,Lewis DA,Ocampo-Sosa AA,Fogarty U,Makrai L,Navas J,Scortti M,Hernández M,Vázquez-Boland JA

更新日期：2006-06-01 00:00:00

abstract：:The effects of UV-B radiation on the heterotrophic nanoflagellate Bodo saltans (Kinetoplastida) were examined under controlled conditions with artificial UV sources and also under natural solar radiation in an oligotrophic lake. In both types of experiments, the characteristic elongated cell morphology of this flagell...

journal_title：Applied and environmental microbiology

authors： Sommaruga R,Oberleiter A,Psenner R

更新日期：1996-12-01 00:00:00

abstract：:Antibody against nivalenol tetraacetate (tetra-Ac-NIV) was prepared by immunization of rabbits with triacetyl-15-pimelate-NIV conjugated to bovine serum albumin. By using tritiated tetra-Ac-NIV as the test ligand, antibody titers were demonstrated as early as 4 weeks after immunization. Useful antibody for radioimmuno...

journal_title：Applied and environmental microbiology

authors： Wang CR,Chu FS

更新日期：1991-04-01 00:00:00

abstract：:Vibrio cholerae identification based on molecular sequence data has been hampered by a lack of sequence variation from the closely related Vibrio mimicus. The two species share many genes coding for proteins, such as ctxAB, and show almost identical 16S DNA coding for rRNA (rDNA) sequences. Primers targeting conserved...

journal_title：Applied and environmental microbiology

authors： Chun J,Huq A,Colwell RR

更新日期：1999-05-01 00:00:00

abstract：:The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594. Results confirmed that the DNA sequence of the 359-bp...

journal_title：Applied and environmental microbiology

authors： Ben-Porath J,Zehr JP

更新日期：1994-03-01 00:00:00

abstract：:Aerobic and facultatively anaerobic bacteria isolated from false-positive, presumptive, total coliform, most-probable-number tests of Chesapeake Bay oyster, water, and sediment samples were characterized and then classified by numerical taxonomy. A total of 538 bacterial strains clustered into 17 phena, the predominan...

journal_title：Applied and environmental microbiology

authors： Hussong D,Damaré JM,Weiner RM,Colwell RR

更新日期：1981-01-01 00:00:00

abstract：:The possible origin of beef contamination and genetic diversity of Escherichia coli populations in beef cattle, on carcasses and ground beef, was examined by using random amplification of polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the fliC gene. E. coli was recovered...

journal_title：Applied and environmental microbiology

authors： Aslam M,Nattress F,Greer G,Yost C,Gill C,McMullen L

更新日期：2003-05-01 00:00:00

abstract：:Bacterial Halanaerobium strains become the dominant persisting microbial community member in produced fluids across geographically distinct hydraulically fractured shales. Halanaerobium is believed to be inadvertently introduced into this environment during the drilling and fracturing process and must therefore tolera...

journal_title：Applied and environmental microbiology

authors： Booker AE,Hoyt DW,Meulia T,Eder E,Nicora CD,Purvine SO,Daly RA,Moore JD,Wunch K,Pfiffner SM,Lipton MS,Mouser PJ,Wrighton KC,Wilkins MJ

更新日期：2019-05-30 00:00:00

abstract：:Reservoir 29 and Lake B are extremely acid lakes (epilimnion pHs of 2.7 and 3.2, respectively), because they receive acidic discharges from coal refuse piles. They differ in that the pH of profundal sediments in Reservoir 29 increased from 2.7 to 3.8 during the period of thermal stratification, whereas permanently ano...

journal_title：Applied and environmental microbiology

authors： Gyure RA,Konopka A,Brooks A,Doemel W

更新日期：1987-09-01 00:00:00