Abstract:
UNLABELLED:Mechanistic studies of many processes in Agrobacterium tumefaciens have been hampered by a lack of genetic tools for characterization of essential genes. In this study, we used a Tn7-based method for inducible control of transcription from an engineered site on the chromosome. We demonstrate that this method enables tighter control of inducible promoters than plasmid-based systems and can be used for depletion studies. The method enables the construction of depletion strains to characterize the roles of essential genes in A. tumefaciens Here, we used the strategy to deplete the alphaproteobacterial master regulator CtrA and found that depletion of this essential gene results in dramatic rounding of cells, which become nonviable. IMPORTANCE:Agrobacterium tumefaciens is a bacterial plant pathogen and natural genetic engineer. Thus, studies of essential processes, including cell cycle progression, DNA replication and segregation, cell growth, and division, may provide insights for limiting disease or improving biotechnology applications.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Figueroa-Cuilan W,Daniel JJ,Howell M,Sulaiman A,Brown PJdoi
10.1128/AEM.01392-16subject
Has Abstractpub_date
2016-07-29 00:00:00pages
5015-25issue
16eissn
0099-2240issn
1098-5336pii
AEM.01392-16journal_volume
82pub_type
杂志文章abstract::The so-called Fe/Mn-oxidizing bacteria have long been recognized for their potential to form extracellular iron hydroxide or manganese oxide structures in aquatic environments. Bacterial species belonging to the genus Gallionella, one type of such bacteria, oxidize iron and produce uniquely twisted extracellular stalk...
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journal_title:Applied and environmental microbiology
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