Internally controlled real-time PCR method for quantitative species-specific detection and vapA genotyping of Rhodococcus equi.

abstract：:A cluster of marine bacteria within the alpha-3 subclass of the class Proteobacteria accounted for up to 28% of the 16S ribosomal DNA (rDNA) sequences in seawater samples from the coast of the southeastern United States. Two independent oligonucleotide probes targeting 16S rDNA of this "marine alpha" cluster indicate ...

journal_title：Applied and environmental microbiology

authors： González JM,Moran MA

更新日期：1997-11-01 00:00:00

abstract：:This paper introduces a novel method for sampling pathogens in natural environments. It uses fabric boot socks worn over walkers' shoes to allow the collection of composite samples over large areas. Wide-area sampling is better suited to studies focusing on human exposure to pathogens (e.g., recreational walking). Thi...

journal_title：Applied and environmental microbiology

authors： Jones NR,Millman C,van der Es M,Hukelova M,Forbes KJ,Glover C,Haldenby S,Hunter PR,Jackson K,O'Brien SJ,Rigby D,Strachan NJC,Williams N,Lake IR,ENIGMA Consortium.

更新日期：2017-06-30 00:00:00

abstract：:Thirty deoxynivalenol-producing F. culmorum strains, isolated from wheat grains, were incubated in vitro and analyzed for trichothecene production. Seventeen strains produced more than 1 ppm of deoxynivalenol and acetyldeoxynivalenol and were considered high-deoxynivalenol-producing strains, whereas 13 F. culmorum str...

journal_title：Applied and environmental microbiology

authors： Bakan B,Giraud-Delville C,Pinson L,Richard-Molard D,Fournier E,Brygoo Y

更新日期：2002-11-01 00:00:00

abstract：:The acI lineage of freshwater Actinobacteria is a cosmopolitan and often numerically dominant member of lake bacterial communities. We conducted a survey of acI 16S rRNA genes and 16S-23S rRNA internal transcribed spacer regions from 18 Wisconsin lakes and used standard nonphylogenetic and phylogenetic statistical app...

journal_title：Applied and environmental microbiology

authors： Newton RJ,Jones SE,Helmus MR,McMahon KD

更新日期：2007-11-01 00:00:00

abstract：:Salmonella enterica serovar Enteritidis was detected in artificially inoculated eggs within 24 h through a rapid monoclonal antibody-based dot blot immunoassay. Detection in poultry and other products required 28 h. Samples were directly enriched in homogenized egg without the need for pre- or postenrichment steps. Se...

journal_title：Applied and environmental microbiology

authors： Yoshimasu MA,Zawistowski J

更新日期：2001-01-01 00:00:00

abstract：:In Pseudomonas aeruginosa, the catabolite repression control (Crc) protein repressed the formation of the blue pigment pyocyanin in response to a preferred carbon source (succinate) by interacting with phzM mRNA, which encodes a key enzyme in pyocyanin biosynthesis. Crc bound to an extended imperfect recognition seque...

journal_title：Applied and environmental microbiology

authors： Huang J,Sonnleitner E,Ren B,Xu Y,Haas D

更新日期：2012-07-01 00:00:00

abstract：:Three Methocel methylcellulose ethers of 1.9 degree of substitution with [14C]methyl labels were shown to be biodegradable using batch-type activated sludge tests. The maximum rate for conversion to 14CO2, attained after 1 week, was only 0.62 mg of methylcellulose/g of mixed liquor volatile solids per day. In 20 days,...

journal_title：Applied and environmental microbiology

authors： Blanchard FA,Takahashi IT,Alexander HC

更新日期：1976-10-01 00:00:00

abstract：:Escherichia coli offers unparalleled engineering capacity in the context of heterologous natural product biosynthesis. However, as with other heterologous hosts, cellular metabolism must be designed or redesigned to support final compound formation. This task is at once complicated and aided by the fact that the cell ...

journal_title：Applied and environmental microbiology

authors： Jiang M,Stephanopoulos G,Pfeifer BA

更新日期：2012-04-01 00:00:00

abstract：:Green mold disease (causal agent, Trichoderma) has resulted in severe crop losses on mushroom farms worldwide in recent years. We analyzed 160 isolates of Trichoderma from mushroom farms for morphological, cultural, and molecular characteristics and classified these isolates into phenotypic groups. The most common gro...

journal_title：Applied and environmental microbiology

authors： Castle A,Speranzini D,Rghei N,Alm G,Rinker D,Bissett J

更新日期：1998-01-01 00:00:00

abstract：:Pili (fimbriae) were observed on cells of each of the five strains of Bradyrhizobium japonicum and the one strain of Rhizobium trifolii examined. Pili on B. japonicum were about 4 nm in diameter and polarly expressed. Piliated cells were estimated by transmission electron microscopy and hydrophobic attachment to polys...

journal_title：Applied and environmental microbiology

authors： Vesper SJ,Bauer WD

更新日期：1986-07-01 00:00:00

abstract：:The spatiotemporal distributions of Aeromonas spp. and fecal coliforms in a cove receiving sewage treatment effluent and draining into a brackish lagoon were studied for 34 months with sampling at six stations. A total of 452 strains of Aeromonas spp. were isolated and identified at the outflow of the treatment system...

journal_title：Applied and environmental microbiology

authors： Monfort P,Baleux B

更新日期：1991-09-01 00:00:00

abstract：:Kinetic parameters and the role of cytochrome c(3) in sulfate, Fe(III), and U(VI) reduction were investigated in Desulfovibrio vulgaris Hildenborough. While sulfate reduction followed Michaelis-Menten kinetics (K(m) = 220 micro M), loss of Fe(III) and U(VI) was first-order at all concentrations tested. Initial reducti...

journal_title：Applied and environmental microbiology

authors： Elias DA,Suflita JM,McInerney MJ,Krumholz LR

更新日期：2004-01-01 00:00:00

abstract：:A total of 221 strains of Lactobacillus isolated from meat and meat products were screened for antagonistic activities under conditions that eliminated the effects of organic acids and hydrogen peroxide. Nineteen strains of Lactobacillus sake, three strains of Lactobacillus plantarum, and one strain of Lactobacillus c...

journal_title：Applied and environmental microbiology

authors： Schillinger U,Lücke FK

更新日期：1989-08-01 00:00:00

abstract：:Inactivation of infectious viruses during drinking water treatment is usually achieved with free chlorine. Many drinking water utilities in the United States now use monochloramine as a secondary disinfectant to minimize disinfectant by-product formation and biofilm growth. The inactivation of human adenoviruses 2, 40...

journal_title：Applied and environmental microbiology

authors： Cromeans TL,Kahler AM,Hill VR

更新日期：2010-02-01 00:00:00

abstract：:The LlaDII restriction/modification (R/M) system was found on the naturally occurring 8.9-kb plasmid pHW393 in Lactococcus lactis subsp. cremoris W39. A 2.4-kb PstI-EcoRI fragment inserted into the Escherichia coli-L. lactis shuttle vector pCI3340 conferred to L. lactis LM2301 and L. lactis SMQ86 resistance against re...

journal_title：Applied and environmental microbiology

authors： Madsen A,Josephsen J

更新日期：1998-07-01 00:00:00

abstract：:During cold storage after milk collection, psychrotrophic bacterial populations dominate the microflora, and their extracellular enzymes, mainly proteases and lipases, contribute to the spoilage of dairy products. The diversity, dynamics, and enzymatic traits of culturable psychrotrophs in raw milk from four farms wer...

journal_title：Applied and environmental microbiology

authors： Hantsis-Zacharov E,Halpern M

更新日期：2007-11-01 00:00:00

abstract：:Growth of Clostridium thermocellum in batch cultures was studied over a broad range of cellobiose concentrations. Cultures displayed important differences in their substrate metabolism as determined by the end product yields. Bacterial growth was severely limited when the initial cellobiose concentration was 0.2 (wt/v...

journal_title：Applied and environmental microbiology

authors： Brener D,Johnson BF

更新日期：1984-05-01 00:00:00

abstract：:Six pairs of pigs were fed a basal diet, a high-fiber diet, and a diet high in corn oil in different sequences to minimize the carry-over effect of diet. After 2 months on each diet, a fecal specimen from each pig was cultured on nonselective medium in roll tubes. Fifty colonies were randomly selected from each fecal ...

journal_title：Applied and environmental microbiology

authors： Moore WE,Moore LV,Cato EP,Wilkins TD,Kornegay ET

更新日期：1987-07-01 00:00:00

abstract：:This study was aimed at assessing whether the repeated exposure of 12 strains of Salmonella spp., Escherichia coli, and Listeria monocytogenes to alternative nonthermal decontamination techniques with UV light (UV-C) and nonthermal atmospheric plasma (NTAP) may cause the emergence of variants showing increased resista...

journal_title：Applied and environmental microbiology

authors： Álvarez-Molina A,de Toro M,Ruiz L,López M,Prieto M,Alvarez-Ordóñez A

更新日期：2020-04-17 00:00:00

abstract：:Two anaerobic bacteria were isolated from polyethylene glycol (PEG)-degrading, methanogenic, enrichment cultures obtained from a municipal sludge digester. One isolate, identified as Desulfovibrio desulfuricans (strain DG2), metabolized oligomers ranging from ethylene glycol (EG) to tetraethylene glycol. The other iso...

journal_title：Applied and environmental microbiology

authors： Dwyer DF,Tiedje JM

更新日期：1986-10-01 00:00:00

abstract：:Arthrobacter viscosus NRRL B-1973 produces its viscous extracellular polysaccharides (EPS) when grown on media containing xylose or enzymatic xylan hydrolysates. Crude EPS formation from xylose averaged 12 g/liter when initial culture pH was adjusted to 8.0 and total nitrogen was limited to 0.03%. Purified EPS from pe...

journal_title：Applied and environmental microbiology

authors： Novak JS,Tanenbaum SW,Nakas JP

更新日期：1992-11-01 00:00:00

abstract：:Quantitation and detection of xylem-limited bacteria with an enzyme-linked immunosorbent assay with a peroxidase conjugate is described. The use of the Trinder reagent (4-aminoantipyrine) allows the determination of extremely small quantities of peroxidase with no precipitate formation or inactivation of the enzyme by...

journal_title：Applied and environmental microbiology

authors： Saunders MS,French WJ

更新日期：1983-08-01 00:00:00

abstract：:Vibrio parahaemolyticus and Vibrio vulnificus are naturally occurring estuarine bacteria and are the leading causes of seafood-associated infections and mortality in the United States. Though multiple-antibiotic-resistant V. parahaemolyticus and V. vulnificus strains have been reported, resistance patterns in vibrios ...

journal_title：Applied and environmental microbiology

authors： Elmahdi S,Parveen S,Ossai S,DaSilva LV,Jahncke M,Bowers J,Jacobs J

更新日期：2018-01-17 00:00:00

abstract：:Chlorella stigmatophora LB 993 was grown in artificial seawater under controlled conditions. The production of cell wall polysaccharides attached to the cells and dissolved in the growth medium was monitored during algal growth. Preliminary characterization of the dissolved polysaccharides of C. stigmatophora and othe...

journal_title：Applied and environmental microbiology

authors： Kaplan D,Christiaen D,Arad SM

更新日期：1987-12-01 00:00:00

abstract：:We report here a biochemical study of resistance to azole antifungal agents in a field isolate (S-27) of a fungal phytopathogen. Isolates of Septoria tritici were compared in vitro, and their responses reflected that observed in the field, with S-27 exhibiting resistance relative to RL2. In untreated cultures, both RL...

journal_title：Applied and environmental microbiology

authors： Joseph-Horne T,Hollomon D,Manning N,Kelly SL

更新日期：1996-01-01 00:00:00

abstract：:Aeromonas caviae W-61, which was isolated from water samples at the Faculty of Agriculture, Tohoku University, produced beta-1, 4-xylanase (1,4-beta-d-xylan xylanohydrolase; EC 3.2.1.8) extracellularly. The xylanase was purified to homogeneity by using DEAE-Sephadex A-50, CM-Sephadex C-50, and Sephadex G-100 column ch...

journal_title：Applied and environmental microbiology

authors： Viet DN,Kamio Y,Abe N,Kaneko J,Izaki K

更新日期：1991-02-01 00:00:00

abstract：:Air-dried soils which were equilibrated under relative humidities greater than 93% or moistened with liquid water showed marked increases in their capacities to oxidize CO to CO(2). Liquid water addition in excess of saturation resulted in lower CO oxidation rates, reflecting the limited diffusion of CO through the aq...

journal_title：Applied and environmental microbiology

authors： Spratt HG,Hubbard JS

更新日期：1981-05-01 00:00:00

abstract：:Polyclonal antibodies of predetermined specificity for pediocin PA-1 (pedA1) have been generated by immunization of rabbits with a chemically synthesized C-terminal fragment of this bacteriocin (PH2) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). The sensitivity and specificity of the PH2-KLH-gener...

journal_title：Applied and environmental microbiology

authors： Martínez JM,Martínez MI,Suárez AM,Herranz C,Casaus P,Cintas LM,Rodríguez JM,Hernández PE

更新日期：1998-11-01 00:00:00

abstract：:Phylogeny and the distribution of symbiotic bacteria in the mixed segment of the wood-eating termite Nasutitermes takasagoensis (Shiraki) were studied. Bacterial 16S rRNA genes (rDNA) were amplified from the mixed segment of the gut by PCR, and two kinds of sequences were identified. The phylogenetic tree was construc...

journal_title：Applied and environmental microbiology

authors： Tokuda G,Yamaoka I,Noda H

更新日期：2000-05-01 00:00:00

abstract：:A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures....

journal_title：Applied and environmental microbiology

authors： Loessner MJ,Schneider A,Scherer S

更新日期：1995-03-01 00:00:00