Abstract:
:Salmonella enterica serovar Enteritidis was detected in artificially inoculated eggs within 24 h through a rapid monoclonal antibody-based dot blot immunoassay. Detection in poultry and other products required 28 h. Samples were directly enriched in homogenized egg without the need for pre- or postenrichment steps. Serovar Enteritidis was detected in the presence of other bacteria when outcompeted 1:400.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Yoshimasu MA,Zawistowski Jdoi
10.1128/AEM.67.1.459-461.2001keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
459-61issue
1eissn
0099-2240issn
1098-5336journal_volume
67pub_type
杂志文章abstract::Leghorn hens over 50 weeks of age were assigned to two treatment groups designated as either unmolted controls or molted. A forced molt was induced by a 9-day feed withdrawal, and each hen was challenged orally with 10(5) Salmonella enteritidis organisms on day 4 of feed withdrawal. On days 4 and 9 of molt, the number...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.5.1919-1923.1999
更新日期:1999-05-01 00:00:00
abstract::Intestinal pathogenic Escherichia coli represents a global health problem for mammals, including humans. At present, diarrheagenic E. coli bacteria are grouped into seven major pathotypes that differ in their virulence factor profiles, severity of clinical manifestations, and prognosis. In this study, we developed and...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02855-06
更新日期:2007-05-01 00:00:00
abstract::A 1.5-kb region immediately downstream of the styABCD operon involved in styrene degradation in Pseudomonas putida CA-3 has been cloned. Sequence analysis revealed a 1,296-bp open reading frame, designated styE, and BLAST P database comparisons of the deduced StyE amino acid sequence revealed 33 to 98% identity with s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.2.1302-1309.2006
更新日期:2006-02-01 00:00:00
abstract::Yeasts grown in the presence of benzoic acid tolerated 40 to 100% higher benzoic acid concentrations than did those grown in the absence of weak-acid-type preservatives. They also accumulated less benzoate in the presence of glucose. In chemostat cultures, benzoic acid reduced growth yield and the rate of cell product...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.8.2091-2095.1988
更新日期:1988-08-01 00:00:00
abstract::The association of Vibrio cholerae with zooplankton has been suggested as an important factor in transmission of human epidemic cholera, and the ability to colonize zooplankton surfaces may play a role in the temporal variation and predominance of the two different serogroups (V. cholerae O1 El Tor and O139) in the aq...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01238-07
更新日期:2007-12-01 00:00:00
abstract::The survival of bacteria exposed to toxic compounds is a multifactorial phenomenon, involving well-known molecular mechanisms of resistance but also less-well-understood mechanisms of tolerance that need to be clarified. In particular, the contribution of biofilm formation to survival in the presence of toxic compound...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02171-08
更新日期:2009-03-01 00:00:00
abstract::3'-Phosphoadenosine-5'-phosphatase (PAPase) is required for the removal of toxic 3'-phosphoadenosine-5'-phosphate (PAP) produced during sulfur assimilation in various eukaryotic organisms. This enzyme is a well-known target of lithium and sodium toxicity and has been used for the production of salt-resistant transgeni...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.1.245-251.2006
更新日期:2006-01-01 00:00:00
abstract::When microbes lack the nutrients necessary for growth, they enter stationary phase. In cases when energy sources are still present in the environment, they must decide whether to continue to use their metabolic program to harvest the available energy. Here we characterized the metabolic response to a variety of types ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00061-14
更新日期:2014-05-01 00:00:00
abstract::Forty-seven strains representing 14 different Bacillus species isolated from clinical and food samples were grown in reconstituted infant milk formulae (IMF) and subsequently assessed for adherence to, invasion of, and cytotoxicity toward HEp-2 and Caco-2 cells. Cell-free supernatant fluids from 38 strains (81%) were ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.67.9.3873-3881.2001
更新日期:2001-09-01 00:00:00
abstract::The influence of trichloroethene (TCE; 0 to 65 mg/liter) and 1,1,1-trichloroethane (1,1,1-TCA; 0 to 103 mg/liter) on methane consumption of a mixed culture of methane-oxidizing bacteria was studied in laboratory batch experiments. Increasing concentrations of TCE or 1,1,1-TCA resulted in decreasing methane consumption...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.8.2488-2493.1990
更新日期:1990-08-01 00:00:00
abstract::Bacterioplankton are fundamental components of marine ecosystems and influence the entire biosphere by contributing to the global biogeochemical cycles of key elements. Yet, there is a significant gap in knowledge about their diversity and specific activities, as well as environmental factors that shape their communit...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00494-17
更新日期:2017-08-17 00:00:00
abstract::Restriction endonuclease fragment patterns of the deoxyribonucleic acid genomes of Autographa californica nuclear polyhedrosis virus (multiply embedded type) and Trichoplusia ni nuclear polyhedrosis virus (multiply embedded type) demonstrate that the two viruses are distinct but closely related variants. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.35.6.1206-1210.1978
更新日期:1978-06-01 00:00:00
abstract::Penicillium decumbens is able to epoxidize cis-propenylphosphonic acid (cPA) to produce the antibiotic fosfomycin [FOM; also referred to as phosphonomycin and (-)-cis-1,2-epoxypropylphosphonic acid], a bioconversion of considerable commercial significance. We sought to improve the efficiency of the process by overexpr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.3.1036-1044.1999
更新日期:1999-03-01 00:00:00
abstract::There is a wealth of evidence indicating that mobile genetic elements can spread in natural microbial communities. However, little is known regarding the fraction of the community that actually engages in this behavior. Here we report on a new approach to quantify the fraction of a bacterial community that is able to ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02713-09
更新日期:2010-07-01 00:00:00
abstract::Currently only five (SEA-SEE) out of 27 known staphylococcal enterotoxins can be analyzed using commercially available kits.Six genes (seg, sei, sem, sen, seo, and seu), encoding putative and undetectable enterotoxins, are located on the enterotoxin gene cluster (egc) which is part of the Staphylococcus aureus genomic...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02662-20
更新日期:2020-12-18 00:00:00
abstract::The effect of cattle manure and slurry application on percolation and survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium was investigated for different soil depths after the addition of water. Four treatments were chosen for the first set of experiments: (i) addition of inoculated farmyar...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01791-08
更新日期:2009-05-01 00:00:00
abstract::Biodegradation experiments with radioactively labeled trichloroethylene showed that 32% of the radioactive carbon was converted to glyoxylic acid, dichloroacetic acid and trichloroacetic acid and that the same percentage was converted to CO2 and CO after 140 h of incubation by a pure culture of a type II methane-utili...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.9.3067-3071.1992
更新日期:1992-09-01 00:00:00
abstract::A biofilm-forming marine bacterium, D2, isolated from the surface of the tunicate Ciona intestinalis, was found to produce a novel, 190-kDa protein with antibacterial activity. The protein contained at least two subunits of 60 and 80 kDa, joined together by noncovalent bonds, and was shown to be released by D2 cells i...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.8.2783-2788.1996
更新日期:1996-08-01 00:00:00
abstract::A xanthanase complex secreted by a consortium of heat-stable, salt-tolerant bacteria includes a lyase that specifically removes terminal pyruvated beta-d-mannose residues from the side chains of xanthan gum. The enzyme was purified to homogeneity from the culture broth following ion-exchange chromatography and gel per...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.9.2523-2528.1991
更新日期:1991-09-01 00:00:00
abstract::The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport su...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00294-06
更新日期:2006-11-01 00:00:00
abstract::When a combination of hydrogen peroxide and hypochlorite was used to surface sterilize rice seeds, a 10(2)- to 10(4)-fold decrease in CFU was observed during the first 15 h after inoculation of the rice rhizosphere organism Burkholderia vietnamiensis TVV75. This artifact could not be eliminated simply by rinsing the s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.7.3046-3052.2001
更新日期:2001-07-01 00:00:00
abstract::Caldalkalibacillus thermarum strain TA2.A1 grew in pH-controlled batch culture containing a fermentable growth substrate (i.e., sucrose) from pH 7.5 to 10.0 with no significant change in the specific growth rate, suggesting that this bacterium was a facultative alkaliphile. However, when strain TA2.A1 was grown on a n...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01639-09
更新日期:2009-12-01 00:00:00
abstract::Mineralization of [U-(14)C]methyl t-butyl ether (MTBE) to (14)CO(2) without accumulation of t-butyl alcohol (TBA) was observed in surface-water sediment microcosms under denitrifying conditions. Methanogenic activity and limited transformation of MTBE to TBA were observed in the absence of denitrification. Results ind...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.4.1975-1978.2001
更新日期:2001-04-01 00:00:00
abstract::In two intertidal marshes, the vertical distribution in the sediment and inhibition by ammonium of methane oxidation were investigated by slurry incubation experiments. The two sites differ in their dominant vegetation type, i.e., reed and bulrush, and in their heights above sea level. The reed site was elevated with ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.12.4734-4740.1997
更新日期:1997-12-01 00:00:00
abstract::Cytolethal distending toxins (CDT) are potent cytotoxins of several Gram-negative pathogenic bacteria, including Escherichia coli, in which five types (CDT-I to CDT-V) have been identified so far. CDT-V is frequently associated with Shiga-toxigenic E. coli (STEC), enterohemorrhagic E. coli (EHEC) O157 strains, and str...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01134-13
更新日期:2013-08-01 00:00:00
abstract::The abundance of quorum quenching (QQ) activity was evaluated in cultivable bacteria obtained from oceanic and estuarine seawater and compared with the frequency of QQ enzyme sequences in the available marine metagenomic collections. The possible role of the high QQ activity found among marine bacteria is discussed. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01266-12
更新日期:2012-09-01 00:00:00
abstract::Carbon dioxide fixation is carried out primarily through the Calvin-Benson-Bassham reductive pentose phosphate cycle, in which ribulose-1, 5-bisphosphate carboxylase/oxygenase (RubisCO) is the key enzyme. The primary structure of the large subunit of form I RubisCO is well conserved; however, four distinct types, A, B...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.6.1913-1921.1996
更新日期:1996-06-01 00:00:00
abstract::The temporal and spatial distribution of Salmonella contamination in the coastal waters of Galicia (northwestern Spain) relative to contamination events with different environmental factors (temperature, wind, hours of sunlight, rainfall, and river flow) were investigated over a 4-year period. Salmonellae were isolate...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.4.2089-2097.2004
更新日期:2004-04-01 00:00:00
abstract::The metabolic conversion of 5-methylfurfural and 2-methylfurfural to furfural by a methanogenic bacterium, Methanococcus sp. strain B, was studied. This bacterium was found to use methylfurfural compounds as a growth substrate and to convert them stoichiometrically to furfural. For every mole of methylfurfurals metabo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.9.3483-3485.1996
更新日期:1996-09-01 00:00:00
abstract::A total of 247 clones of 16S rRNA genes from microorganisms captured by 0.2- and 0.1-microm-pore-size filters from sedimentary and granite rock aquifers were amplified and yielded 37 operational taxonomic units (OTUs). Fifteen OTUs captured by 0.1-microm-pore-size filters were affiliated with the candidate divisions O...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.2.1084-1088.2005
更新日期:2005-02-01 00:00:00