Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR.

abstract：:To measure genetic variation within and among populations of the bloom-forming cyanobacterium Microcystis aeruginosa, we surveyed a suite of lakes in the southern peninsula of Michigan that vary in productivity (total phosphorus concentrations of approximately 10 to 100 microg liter(-1)). Survival of M. aeruginosa iso...

journal_title：Applied and environmental microbiology

authors： Wilson AE,Sarnelle O,Neilan BA,Salmon TP,Gehringer MM,Hay ME

更新日期：2005-10-01 00:00:00

abstract：:The genetic diversity of Desulfovibrio species in environmental samples was determined by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified [NiFe] hydrogenase gene fragments. Five different PCR primers were designed after comparative analysis of [NiFe] hydrogenase gene sequences from three Desulfovibrio ...

journal_title：Applied and environmental microbiology

authors： Wawer C,Muyzer G

更新日期：1995-06-01 00:00:00

abstract：:The susceptibility of a strain of Legionella pneumophila to disinfection by an organic halamine, free chlorine, and a mixture of the organic halamine and free chlorine was assessed. The organic halamine was found to have superior stability in solution and to exhibit adequate disinfectant potential over a period of 1 m...

journal_title：Applied and environmental microbiology

authors： Swango LJ,Wilt GR,Killen AD,Williams DE,Worley SD

更新日期：1987-12-01 00:00:00

abstract：:The composition of predominant soil bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural production at different time points, and one currently fertilized plot represented different stages of grassland succession. Since fertilization and agricultura...

journal_title：Applied and environmental microbiology

authors： Felske A,Wolterink A,Van Lis R,De Vos WM,Akkermans AD

更新日期：2000-09-01 00:00:00

abstract：:A method was developed to detect NO- or N(2)O-producing bacteria in solid or liquid medium by their ability to oxidize the redox indicator resazurin from its reduced colorless form to its oxidized pink form. The method was sensitive to as little as 35 nM N(2)O or 0.5 nM NO. Ninety-one percent of the colonies that oxid...

journal_title：Applied and environmental microbiology

authors： Jenneman GE,Montgomery AD,McInerney MJ

更新日期：1986-04-01 00:00:00

abstract：:A metagenomic library of a petroleum-contaminated soil was constructed in a fosmid vector that allowed heterologous expression of metagenomic DNA. The library, consisting of 6.5 Gb of metagenomic DNA, was screened for extradiol dioxygenase (Edo) activity using catechol and 2,3-dihydroxybiphenyl as the substrates. Fift...

journal_title：Applied and environmental microbiology

authors： Terrón-González L,Martín-Cabello G,Ferrer M,Santero E

更新日期：2016-04-04 00:00:00

abstract：:The fungus Fusarium oxysporum produced a D-amino acid oxidase (EC 1. 4.3.3) in a medium containing glucose as the carbon and energy source and ammonium sulfate as the nitrogen source. The specific D-amino acid oxidase activity was increased up to 12.5-fold with various D-amino acids or their corresponding derivatives ...

journal_title：Applied and environmental microbiology

authors： Gabler M,Fischer L

更新日期：1999-08-01 00:00:00

abstract：:Acidophilic archaea of the archaeal Richmond Mine acidophilic nanoorganisms (ARMAN) group from the uncultured candidate phylum "Candidatus Micrarchaeota" have small genomes and cell sizes and are known to be metabolically dependent and physically associated with their Thermoplasmatales hosts. However, phylogenetically...

journal_title：Applied and environmental microbiology

authors： Kadnikov VV,Savvichev AS,Mardanov AV,Beletsky AV,Chupakov AV,Kokryatskaya NM,Pimenov NV,Ravin NV

更新日期：2020-11-10 00:00:00

abstract：:Carbohydrate components (simple sugars and polysaccharides) of cell walls of pearl millet (Pennisetum americanum L., cv. Gahi) were studied as potential substrates for the root-associated diazotroph Azospirillum brasiliense Sp. 7. Simple sugars were utilized, but no evidence was obtained to support the suggestion that...

journal_title：Applied and environmental microbiology

authors： Myers ML,Hubbell DH

更新日期：1987-12-01 00:00:00

abstract：:Recombinant strains of Pseudomonas putida KT2440 carrying genetic expression cassettes with xylene oxygenase- and styrene monooxygenase-encoding genes on their chromosomes could be induced in shaking-flask experiments to specific activities that rivaled those of multicopy-plasmid-based Escherichia coli recombinants. S...

journal_title：Applied and environmental microbiology

authors： Panke S,de Lorenzo V,Kaiser A,Witholt B,Wubbolts MG

更新日期：1999-12-01 00:00:00

abstract：:A simple gel immunodiffusion agar procedure was developed for detecting toxigenic strains of Clostridium botulinum type A. The method consisted of overlaying colonies grown on thin-layer tryptone-peptone-glucose-yeast extract agar with gel diffusion agar containing desired levels of C. botulinum type A antitoxin. Conc...

journal_title：Applied and environmental microbiology

authors： Ferreira JL,Hamdy MK,Zapatka FA,Hebert WO

更新日期：1981-12-01 00:00:00

abstract：:Purified manganese peroxidase (MnP) from the white-rot basidiomycete Phlebia radiata was found to convert in vitro milled pine wood (MPW) suspended in an aqueous reaction solution containing Tween 20, Mn(2+), Mn-chelating organic acid (malonate), and a hydrogen peroxide-generating system (glucose-glucose oxidase). The...

journal_title：Applied and environmental microbiology

authors： Hofrichter M,Lundell T,Hatakka A

更新日期：2001-10-01 00:00:00

abstract：:Lysine iron agar, modified by the addition of bile salts, novobiocin, lactose, and sucrose, is a valuable plating medium for the isolation of Salmonella, including H2S-negative strains. ...

journal_title：Applied and environmental microbiology

authors： Rappold H,Bolderdijk RF

更新日期：1979-07-01 00:00:00

abstract：:Aerobic and facultatively anaerobic bacteria isolated from false-positive, presumptive, total coliform, most-probable-number tests of Chesapeake Bay oyster, water, and sediment samples were characterized and then classified by numerical taxonomy. A total of 538 bacterial strains clustered into 17 phena, the predominan...

journal_title：Applied and environmental microbiology

authors： Hussong D,Damaré JM,Weiner RM,Colwell RR

更新日期：1981-01-01 00:00:00

abstract：:An organism tentatively identified as Ralstonia eutropha was isolated from enrichment cultures containing tetrahydrofurfuryl alcohol (THFA) as the sole source of carbon and energy. The strain was able to tolerate up to 200 mM THFA in mineral salt medium. The degradation was initiated by an inducible ferricyanide-depen...

journal_title：Applied and environmental microbiology

authors： Zarnt G,Schräder T,Andreesen JR

更新日期：1997-12-01 00:00:00

abstract：:A homokaryotic, clavine alkaloid-producing strain of ergot, Claviceps sp. strain SD 58, was used in an attempt to demonstrate parasexuality. Genetically marked auxotrophic strains were produced by mutation with N-methyl-N'-nitro-N-nitrosoguanidine. Protoplast fusion of pairs of unlike doubly auxotrophic strains and is...

journal_title：Applied and environmental microbiology

authors： Brauer KL,Robbers JE

更新日期：1987-01-01 00:00:00

abstract：:Motile phototrophic consortia are highly regular associations in which numerous cells of green sulfur bacteria surround a flagellated colorless beta-proteobacterium in the center. To date, seven different morphological types of such consortia have been described. In addition, two immotile associations involving green ...

journal_title：Applied and environmental microbiology

authors： Glaeser J,Overmann J

更新日期：2004-08-01 00:00:00

abstract：:Five strains of Aspergillus fumigatus (A, B, D, H, and K) isolated from sawmills were used to expose groups of three rats by intratracheal intubation. The dose was 10(9) spores per rat. At 48 h after administration, two rats from the D group and all rats from the K group died with symptoms of strong dyspnea and tachyp...

journal_title：Applied and environmental microbiology

authors： Land CJ,Sostarić B,Fuchs R,Lundström H,Hult K

更新日期：1989-11-01 00:00:00

abstract：:The inhibitory effects of sterigmatocystin, O-methylsterigmatocystin, and 5,6-dimethoxysterigmatocystin on the ATP synthesis system in mitochondria were compared with that of aflatoxin B1, which disturbs the respiratory chain in mitochondria. Sterigmatocystin and 5,6-dimethoxysterigmatocystin were found to uncouple th...

journal_title：Applied and environmental microbiology

authors： Kawai K,Nakamaru T,Nozawa Y,Maebayashi Y,Yamazaki M,Natori S

更新日期：1984-11-01 00:00:00

abstract：:Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, wh...

journal_title：Applied and environmental microbiology

authors： Degering C,Eggert T,Puls M,Bongaerts J,Evers S,Maurer KH,Jaeger KE

更新日期：2010-10-01 00:00:00

abstract：:The uncultivated bacterial endosymbionts of the hydrothermal vent tubeworm Riftia pachyptila play a central role in providing their host with fixed carbon. While this intimate association between host and symbiont indicates tight integration and coordination of function via cellular communication mechanisms, no such s...

journal_title：Applied and environmental microbiology

authors： Hughes DS,Felbeck H,Stein JL

更新日期：1997-09-01 00:00:00

abstract：:Cellulase and xylanase cDNAs were isolated from a cDNA library of the polycentric anaerobic fungus Orpinomyces sp. strain PC-2 constructed in Escherichia coli. The cellulase cDNA (celB) was 1.8 kb long with an open reading frame (ORF) coding for a polypeptide of 471 amino acids, and the xylanase cDNA (xynA) was 1.2 kb...

journal_title：Applied and environmental microbiology

authors： Li XL,Chen H,Ljungdahl LG

更新日期：1997-02-01 00:00:00

abstract：:Three Rhizobium japonicum strains and two slow-growing cowpea-type Rhizobium strains were found to remain viable and able to rapidly modulate their respective hosts after being stored in purified water at ambient temperatures for periods of 1 year and longer. Three fast-growing Rhizobium species did not remain viable ...

journal_title：Applied and environmental microbiology

authors： Crist DK,Wyza RE,Mills KK,Bauer WD,Evans WR

更新日期：1984-05-01 00:00:00

abstract：:Selective adhesion to only certain epithelia is particularly common among the bacterial members of the indigenous microflora of mammals. We have found that the stratified squamous epithelium of the nonsecreting area of horse stomach is colonized by gram-positive rods. The microscopic features of a dense layer of these...

journal_title：Applied and environmental microbiology

authors： Yuki N,Shimazaki T,Kushiro A,Watanabe K,Uchida K,Yuyama T,Morotomi M

更新日期：2000-11-01 00:00:00

abstract：:The distribution of methanogenic and sulfate-reducing bacteria was examined in sediments from three sites off the coast of eastern Connecticut and five sites in Long Island Sound. Both bacterial groups were detected at all sites. Three distributional patterns were observed: (i) four sites exhibited methanogenic and su...

journal_title：Applied and environmental microbiology

authors： Hines ME,Buck JD

更新日期：1982-02-01 00:00:00

abstract：:The antimicrobial activities of sucrose monolaurate and a novel ester, lactose monolaurate (LML), were tested. Gram-positive bacteria were more susceptible than Gram-negative bacteria to both esters. The minimal bactericidal concentrations of LML were 5 to 9.5 mM for Listeria monocytogenes isolates and 0.2 to 2 mM for...

journal_title：Applied and environmental microbiology

authors： Wagh A,Shen S,Shen FA,Miller CD,Walsh MK

更新日期：2012-05-01 00:00:00

abstract：:Anaerobic degradation of 2-methylnaphthalene was investigated with a sulfate-reducing enrichment culture. Metabolite analyses revealed two groups of degradation products. The first group comprised two succinic acid adducts which were identified as naphthyl-2-methyl-succinic acid and naphthyl-2-methylene-succinic acid ...

journal_title：Applied and environmental microbiology

authors： Annweiler E,Materna A,Safinowski M,Kappler A,Richnow HH,Michaelis W,Meckenstock RU

更新日期：2000-12-01 00:00:00

abstract：:A real-time PCR assay was developed for the quantitative detection of Campylobacter jejuni in foods after enrichment culture. The specificity of the assay for C. jejuni was demonstrated with a diverse range of Campylobacter species, related organisms, and unrelated genera. The assay had a linear range of quantificatio...

journal_title：Applied and environmental microbiology

authors： Sails AD,Fox AJ,Bolton FJ,Wareing DR,Greenway DL

更新日期：2003-03-01 00:00:00

abstract：:A field collected population of Plutella xylostella (SERD4) was selected in the laboratory with Bacillus thuringiensis endotoxins Cry1Ac (Cry1Ac-SEL) and Cry1Ab (Cry1Ab-SEL). Both subpopulations showed similar phenotypes: high resistance to the Cry1A toxins and little cross-resistance to Cry1Ca or Cry1D. A previous an...

journal_title：Applied and environmental microbiology

authors： Sayyed AH,Gatsi R,Ibiza-Palacios MS,Escriche B,Wright DJ,Crickmore N

更新日期：2005-11-01 00:00:00

abstract：:Symbiotic microorganisms have been found in the hemolymph (blood) of many aquatic invertebrates, such as crabs, shrimp, and oysters. Hemolymph is a critical site in the host immune response. Currently, studies on hemolymph microorganisms are mostly performed with culture-dependent strategies using selective media (e.g...

journal_title：Applied and environmental microbiology

authors： Zhang X,Sun Z,Zhang X,Zhang M,Li S

更新日期：2018-04-02 00:00:00