Engineering of a stable whole-cell biocatalyst capable of (S)-styrene oxide formation for continuous two-liquid-phase applications.

Abstract:

:Recombinant strains of Pseudomonas putida KT2440 carrying genetic expression cassettes with xylene oxygenase- and styrene monooxygenase-encoding genes on their chromosomes could be induced in shaking-flask experiments to specific activities that rivaled those of multicopy-plasmid-based Escherichia coli recombinants. Such strains maintained the introduced styrene oxidation activity in continuous two-liquid-phase cultures for at least 100 generations, although at a lower level than in the shaking-flask experiments. The data suggest that placement of target genes on the chromosome might be a suitable route for the construction of segregationally stable and highly active whole-cell biocatalysts.

journal_name

Appl Environ Microbiol

authors

Panke S,de Lorenzo V,Kaiser A,Witholt B,Wubbolts MG

doi

10.1128/AEM.65.12.5619-5623.1999

keywords:

subject

Has Abstract

pub_date

1999-12-01 00:00:00

pages

5619-23

issue

12

eissn

0099-2240

issn

1098-5336

journal_volume

65

pub_type

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