Effect of promoter modification on mosquitocidal cryIVB gene expression in Synechococcus sp. strain PCC 7942.

abstract：:The crenarchaeon Sulfolobus acidocaldarius has been described to synthesize trehalose via the maltooligosyltrehalose synthase (TreY) and maltooligosyltrehalose trehalohydrolase (TreZ) pathway, and the trehalose glycosyltransferring synthase (TreT) pathway has been predicted. Deletion mutant analysis of strains with si...

journal_title：Applied and environmental microbiology

authors： Stracke C,Meyer BH,Hagemann A,Jo E,Lee A,Albers SV,Cha J,Bräsen C,Siebers B

更新日期：2020-11-24 00:00:00

abstract：:Escherichia coli O157:H7 is a leading cause of food-borne illness. This human pathogen produces Shiga toxins (Stx1 and Stx2) which inhibit protein synthesis by inactivating ribosome function. The present study describes a novel cell-based assay to detect Stx2 and inhibitors of toxin activity. A Vero cell line harborin...

journal_title：Applied and environmental microbiology

authors： Quiñones B,Massey S,Friedman M,Swimley MS,Teter K

更新日期：2009-03-01 00:00:00

abstract：:The presence of neurotoxic species within the genus Alexandrium along the U.S. coastline has raised concern of potential poisoning through the consumption of contaminated seafood. Paralytic shellfish toxins (PSTs) detected in shellfish provide evidence that these harmful events have increased in frequency and severity...

journal_title：Applied and environmental microbiology

authors： Garneau MÈ,Schnetzer A,Countway PD,Jones AC,Seubert EL,Caron DA

更新日期：2011-11-01 00:00:00

abstract：:A total of 114 gram-negative fecal isolates from domestic pigs in herds with different histories of antimicrobial agent exposure were screened for the presence of plasmid DNA and specific tetracycline resistance determinants. More than 84% of the isolates harbored plasmid DNA, which ranged in size from 2.1 to 186 kb. ...

journal_title：Applied and environmental microbiology

authors： Lee C,Langlois BE,Dawson KA

更新日期：1993-05-01 00:00:00

abstract：:Recombinant DNA clones carrying high-copy or low-copy sequences from Aspergillus nidulans and Neurospora crassa were used to identify restriction fragment length polymorphisms (RFLPs) diagnostic for members of the A. flavus group: A. flavus, A. parasiticus, and A. nomius. These fungi were resolved into three distinct ...

journal_title：Applied and environmental microbiology

authors： Moody SF,Tyler BM

更新日期：1990-08-01 00:00:00

abstract：:The genus Listeria comprises food-borne pathogens associated with severe infections and a high mortality rate. Endolysins from bacteriophages infecting Listeria are promising tools for both their detection and control. These proteins feature a modular organization, consisting of an N-terminal enzymatically active doma...

journal_title：Applied and environmental microbiology

authors： Schmelcher M,Shabarova T,Eugster MR,Eichenseher F,Tchang VS,Banz M,Loessner MJ

更新日期：2010-09-01 00:00:00

abstract：:Green mold disease (causal agent, Trichoderma) has resulted in severe crop losses on mushroom farms worldwide in recent years. We analyzed 160 isolates of Trichoderma from mushroom farms for morphological, cultural, and molecular characteristics and classified these isolates into phenotypic groups. The most common gro...

journal_title：Applied and environmental microbiology

authors： Castle A,Speranzini D,Rghei N,Alm G,Rinker D,Bissett J

更新日期：1998-01-01 00:00:00

abstract：:Fungal multicopper oxidases have many potential industrial applications, since they perform reactions under mild conditions. We isolated a phenol oxidase from the fungus Acremonium murorum var. murorum that was capable of decolorizing plant chromophores (such as anthocyanins). This enzyme is of interest in laundry-cle...

journal_title：Applied and environmental microbiology

authors： Gouka RJ,van der Heiden M,Swarthoff T,Verrips CT

更新日期：2001-06-01 00:00:00

abstract：:Eukaryotes may influence pollutant degradation processes in groundwater ecosystems by activities such as predation on bacteria and recycling of nutrients. Culture-independent community profiling and phylogenetic analysis of 18S rRNA gene fragments, as well as culturing, were employed to obtain insight into the sedimen...

journal_title：Applied and environmental microbiology

authors： Brad T,Braster M,van Breukelen BM,van Straalen NM,Röling WF

更新日期：2008-07-01 00:00:00

abstract：:A bacterial strain able to transform iprodione was isolated from a fast iprodione-degrading soil by enrichment procedures. Transformation was detected through 3,5-dichloroaniline production as measured by a rapid colorimetric method. The strain, MA6, was tentatively identified as an Arthrobacter sp. When it was incuba...

journal_title：Applied and environmental microbiology

authors： Athiel P,Alfizar,Mercadier C,Vega D,Bastide J,Davet P,Brunel B,Cleyet-Marel JC

更新日期：1995-09-01 00:00:00

abstract：:Listeria monocytogenes is a foodborne pathogen whose survival in food processing environments may be associated with its tolerance to desiccation. To probe the molecular mechanisms used by this bacterium to adapt to desiccation stress, a transposon library of 11,700 L. monocytogenes mutants was screened, using a micro...

journal_title：Applied and environmental microbiology

authors： Hingston PA,Piercey MJ,Truelstrup Hansen L

更新日期：2015-08-15 00:00:00

abstract：:A total of 765 Escherichia coli isolates from point and nonpoint sources were collected from the Apalachicola National Estuarine Research Reserve, and their multiple-antibiotic-resistance (MAR) profiles were determined with 10 antibiotics. E. coli isolates from point sources showed significantly greater resistance (P ...

journal_title：Applied and environmental microbiology

authors： Parveen S,Murphree RL,Edmiston L,Kaspar CW,Portier KM,Tamplin ML

更新日期：1997-07-01 00:00:00

abstract：:Streptococcus agalactiae (group B Streptococcus [GBS]) is a cause of severe infections, particularly during the newborn period. While methods exist for generating chromosomal mutations in GBS, they are cumbersome and inefficient and present significant challenges if the goal is to study subtle mutations, such as singl...

journal_title：Applied and environmental microbiology

authors： Hooven TA,Bonakdar M,Chamby AB,Ratner AJ

更新日期：2019-03-22 00:00:00

abstract：:We have used a sintered glass bead core to simulate the spaces and surfaces of reservoir rock in studies of the bacterial plugging phenomenon that affects waterflood oil recovery operations. The passage of pure or mixed natural populations of bacteria through this solid matrix was initially seen to promote the formati...

journal_title：Applied and environmental microbiology

authors： Shaw JC,Bramhill B,Wardlaw NC,Costerton JW

更新日期：1985-03-01 00:00:00

abstract：:In this article, a new mechanism influencing the transport of microorganisms through unsaturated porous media is examined, and a new method for directly visualizing bacterial behavior within a porous medium under controlled chemical and flow conditions is introduced. Resting cells of hydrophilic and relatively hydroph...

journal_title：Applied and environmental microbiology

authors： Wan J,Wilson JL,Kieft TL

更新日期：1994-02-01 00:00:00

abstract：:In this work we investigated the antibacterial properties of differently shaped silver nanoparticles against the gram-negative bacterium Escherichia coli, both in liquid systems and on agar plates. Energy-filtering transmission electron microscopy images revealed considerable changes in the cell membranes upon treatme...

journal_title：Applied and environmental microbiology

authors： Pal S,Tak YK,Song JM

更新日期：2007-03-01 00:00:00

abstract：:The natural products of both eremofortin C (EC) and PR toxin are secondary metabolites of Penicillium roqueforti. Because the chemical structures of EC and PR toxin are closely related to each other and differ only by a hydroxyl functional group in EC and an aldehyde functional group in PR toxin at the C-12 position, ...

journal_title：Applied and environmental microbiology

authors： Li SY,Chang SC,Wei RD

更新日期：1985-09-01 00:00:00

abstract：:The degradation of epichlorohydrin (3-chloropropylene oxide or 1-chloro-2,3-epoxypropane) by whole-cell suspensions of Xanthobacter strain Py2 was investigated. Cell suspensions prepared from cultures grown with propylene as the carbon source readily degraded epichlorohydrin. The ability to degrade epichlorohydrin cor...

journal_title：Applied and environmental microbiology

authors： Small FJ,Tilley JK,Ensign SA

更新日期：1995-04-01 00:00:00

abstract：:Sequence analysis of the five genes (gutRMCBA) downstream from the previously described sorbitol-6-phosphate dehydrogenase-encoding Lactobacillus casei gutF gene revealed that they constitute a sorbitol (glucitol) utilization operon. The gutRM genes encode putative regulators, while the gutCBA genes encode the EIIC, E...

journal_title：Applied and environmental microbiology

authors： Alcántara C,Sarmiento-Rubiano LA,Monedero V,Deutscher J,Pérez-Martínez G,Yebra MJ

更新日期：2008-09-01 00:00:00

abstract：:Biological dinitrogen fixation in mangrove communities of the Tampa Bay region of South Florida was investigated using the acetylene reduction technique. Low rates of acetylene reduction (0.01 to 1.84 nmol of C(2)H(4)/g [wet weight] per h) were associated with plant-free sediments, while plant-associated sediments gav...

journal_title：Applied and environmental microbiology

authors： Zuberer DA,Silver WS

更新日期：1978-03-01 00:00:00

abstract：:A homolog of the clpC ATPase gene was identified in the genome of Bifidobacterium breve UCC 2003. Since this gene is very well conserved among eubacteria, we employed a PCR-based approach using primers based on highly conserved regions of ClpC proteins in order to identify homologous genes in other bifidobacterial spe...

journal_title：Applied and environmental microbiology

authors： Ventura M,Fitzgerald GF,van Sinderen D

更新日期：2005-10-01 00:00:00

abstract：:A nonmotile, gram-positive, spherical organism was isolated from the intestinal tracts of rabbits. Both hydrogen and methanol were required for growth. No methane was produced from hydrogen-carbon dioxide, formate, acetate, methylamines, ethanol, or isopropanol. The optimum pH was 6.8, and the optimum temperature was ...

journal_title：Applied and environmental microbiology

authors： Biavati B,Vasta M,Ferry JG

更新日期：1988-03-01 00:00:00

abstract：:The bacterial pathogen Vibrio vulnificus is found naturally in brackish coastal waters but can be greatly concentrated by filter-feeding organisms such as shellfish. Numerous experiments in which exogenous V. vulnificus cells are added to oysters in an attempt to measure uptake and depuration have been performed. In n...

journal_title：Applied and environmental microbiology

authors： Froelich B,Oliver J

更新日期：2013-09-01 00:00:00

abstract：:A 1.5-kb region immediately downstream of the styABCD operon involved in styrene degradation in Pseudomonas putida CA-3 has been cloned. Sequence analysis revealed a 1,296-bp open reading frame, designated styE, and BLAST P database comparisons of the deduced StyE amino acid sequence revealed 33 to 98% identity with s...

journal_title：Applied and environmental microbiology

authors： Mooney A,O'Leary ND,Dobson AD

更新日期：2006-02-01 00:00:00

abstract：:The concentration of 2-aminoethylphosphonic acid has been measured in seven genera of rumen ciliate protozoa. Expressed as milligrams per gram of total nitrogen, 2-aminoethylphosphonic acid concentrations ranged from 17.2 in Ophryoscolex spp. to 72.4 in Eremoplastron spp. ...

journal_title：Applied and environmental microbiology

authors： Whitelaw FG,Bruce LA,Eadie JM,Shand WJ

更新日期：1983-10-01 00:00:00

abstract：:A longstanding awareness in generating resistance to common antimicrobial therapies by Gram-negative bacteria has made them a major threat to global health. The application of antimicrobial peptides as a therapeutic agent would be a great opportunity to combat bacterial diseases. Here, we introduce a new antimicrobial...

journal_title：Applied and environmental microbiology

authors： Modiri S,Kasra Kermanshahi R,Soudi MR,Arab SS,Khammari A,Cousineau B,Vali H,Zahiri HS,Noghabi KA

更新日期：2020-05-05 00:00:00

abstract：:Starting with a strain of Bacillus cereus excreting about 40-fold more beta-amylase than does the original wild-type strain, we isolated, after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a strain designated BQ10-S1 SpoIII which showed under optimal conditions a further 5.5-fold increase in beta-amylase act...

journal_title：Applied and environmental microbiology

authors： Nanmori T,Numata Y,Shinke R

更新日期：1987-04-01 00:00:00

abstract：:Recent studies have reported high levels of fecal indicator enterococci in marine beach sand. This study aimed to determine the spatial and temporal variation of enterococcal abundance and to evaluate its relationships with microbial community parameters in Hawaii beach sand and water. Sampling at 23 beaches on the Is...

journal_title：Applied and environmental microbiology

authors： Cui H,Yang K,Pagaling E,Yan T

更新日期：2013-06-01 00:00:00

abstract：:The beta-xylosidase-encoding xlnD gene of Aspergillus niger 90196 was amplified by the PCR technique from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified to contain a 2,412-bp open reading frame that encodes a 804-amino-acid propeptide. The 778-...

journal_title：Applied and environmental microbiology

authors： La Grange DC,Pretorius IS,Claeyssens M,van Zyl WH

更新日期：2001-12-01 00:00:00

abstract：:The type III secretion system (T3SS) is a specialized secretion apparatus that is commonly used by many plant and animal pathogenic bacteria to deliver proteins, termed effectors, to the interior of the host cells. These effectors suppress host defenses and interfere with signal transduction pathways to promote infect...

journal_title：Applied and environmental microbiology

authors： Jiménez-Guerrero I,Pérez-Montaño F,Medina C,Ollero FJ,López-Baena FJ

更新日期：2017-02-15 00:00:00