Compatibility of rhizobial genotypes within natural populations of Rhizobium leguminosarum biovar viciae for nodulation of host legumes.

Abstract:

:Populations of Rhizobium leguminosarum biovar viciae were sampled from two bulk soils, rhizosphere, and nodules of host legumes, fava bean (Vicia faba) and pea (Pisum sativum) grown in the same soils. Additional populations nodulating peas, fava beans, and vetches (Vicia sativa) grown in other soils and fava bean-nodulating strains from various geographic sites were also analyzed. The rhizobia were characterized by repetitive extragenomic palindromic-PCR fingerprinting and/or PCR-restriction fragment length polymorphism (RFLP) of 16S-23S ribosomal DNA intergenic spacers as markers of the genomic background and PCR-RFLP of a nodulation gene region, nodD, as a marker of the symbiotic component of the genome. Pairwise comparisons showed differences among the genetic structures of the bulk soil, rhizosphere, and nodule populations and in the degree of host specificity within the Vicieae cross-inoculation group. With fava bean, the symbiotic genotype appeared to be the preponderant determinant of the success in nodule occupancy of rhizobial genotypes independently of the associated genomic background, the plant genotype, and the soil sampled. The interaction between one particular rhizobial symbiotic genotype and fava bean seems to be highly specific for nodulation and linked to the efficiency of nitrogen fixation. By contrast with bulk soil and fava bean-nodulating populations, the analysis of pea-nodulating populations showed preferential associations between genomic backgrounds and symbiotic genotypes. Both components of the rhizobial genome may influence competitiveness for nodulation of pea, and rhizosphere colonization may be a decisive step in competition for nodule occupancy.

journal_name

Appl Environ Microbiol

authors

Laguerre G,Louvrier P,Allard MR,Amarger N

doi

10.1128/aem.69.4.2276-2283.2003

keywords:

subject

Has Abstract

pub_date

2003-04-01 00:00:00

pages

2276-83

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

69

pub_type

杂志文章
  • Vitamin K Analogs Influence the Growth and Virulence Potential of Enterohemorrhagic Escherichia coli.

    abstract::Enterohemorrhagic Escherichia coli (EHEC) causes serious foodborne disease worldwide. It produces the very potent Shiga toxin 2 (Stx2). The Stx2-encoding genes are located on a prophage, and production of the toxin is linked to the synthesis of Stx phages. There is, currently, no good treatment for EHEC infections, as...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00583-20

    authors: Kijewski A,Witsø IL,Iversen H,Rønning HT,L'Abée-Lund T,Wasteson Y,Lindbäck T,Aspholm M

    更新日期:2020-11-24 00:00:00

  • Galactokinase activity in Streptococcus thermophilus.

    abstract::ATP-dependent phosphorylation of [14C]galactose by 11 strains of Streptococcus thermophilus indicated that these organisms possessed the Leloir enzyme, galactokinase (galK). Activities were 10 times higher in fully induced, galactose-fermenting (Gal+) strains than in galactose-nonfermenting (Gal-) strains. Lactose-gro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.4.777-780.1985

    authors: Hutkins R,Morris HA,McKay LL

    更新日期:1985-10-01 00:00:00

  • Role of fadR and atoC(Con) mutations in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesis in recombinant pha+ Escherichia coli.

    abstract::Recombinant Escherichia coli fadR atoC(Con) mutants containing the polyhydroxyalkanoate (PHA) biosynthesis genes from Alcaligenes eutrophus are able to incorporate significant levels of 3-hydroxyvalerate (3HV) into the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)]. We have used E. coli fadR (F...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.7.2487-2492.1995

    authors: Rhie HG,Dennis D

    更新日期:1995-07-01 00:00:00

  • Microbial desulfurization of alkylated dibenzothiophenes from a hydrodesulfurized middle distillate by Rhodococcus erythropolis I-19.

    abstract::Rhodococcus erythropolis I-19, containing multiple copies of key dsz genes, was used to desulfurize alkylated dibenzothiophenes (Cx-DBTs) found in a hydrodesulfurized middle-distillate petroleum (MD 1850). Initial desulfurization rates of dibenzothiophene (DBT) and MD 1850 by I-19 were 5.0 and 2.5 micromol g dry cell ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.11.4967-4972.1999

    authors: Folsom BR,Schieche DR,DiGrazia PM,Werner J,Palmer S

    更新日期:1999-11-01 00:00:00

  • Physical and Functional Interplay between MazF1Bif and Its Noncognate Antitoxins from Bifidobacterium longum.

    abstract::Bifidobacterium longum strain JDM301, a widely used commercial strain in China, encodes at least two MazEF-like modules and one RelBE-like toxin-antitoxin (TA) system in its chromosome, designated MazE1F1Bif, MazE2F2Bif, and RelBEBif, respectively. Bacterial TA systems play an important role in several stress response...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03232-16

    authors: Wei Y,Li Y,Yang F,Wu Q,Liu D,Li X,Hua H,Liu X,Wang Y,Zheng K,Tang R

    更新日期:2017-04-17 00:00:00

  • Multifunctional Acidocin 4356 Combats Pseudomonas aeruginosa through Membrane Perturbation and Virulence Attenuation: Experimental Results Confirm Molecular Dynamics Simulation.

    abstract::A longstanding awareness in generating resistance to common antimicrobial therapies by Gram-negative bacteria has made them a major threat to global health. The application of antimicrobial peptides as a therapeutic agent would be a great opportunity to combat bacterial diseases. Here, we introduce a new antimicrobial...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00367-20

    authors: Modiri S,Kasra Kermanshahi R,Soudi MR,Arab SS,Khammari A,Cousineau B,Vali H,Zahiri HS,Noghabi KA

    更新日期:2020-05-05 00:00:00

  • Discrimination of species in the genus Listeria by Fourier transform infrared spectroscopy and canonical variate analysis.

    abstract::Infrared spectra of type cultures of the six recognized species of the genus Listeria and of Listeria grayi subsp. murrayi were recorded. By use of a library of 59 spectra, comprising at least six replicates of each type, discrimination by canonical variate analysis of the spectral amplitudes allowed all of the spectr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.1.377-378.1995

    authors: Holt C,Hirst D,Sutherland A,MacDonald F

    更新日期:1995-01-01 00:00:00

  • Inhibition of mitogen-induced blastogenesis in human lymphocytes by T-2 toxin and its metabolites.

    abstract::Concentrations of T-2, HT-2, 3'-OH T-2, 3'-OH HT-2, T-2 triol, and T-2 tetraol toxins which inhibited [3H]thymidine uptake in mitogen-stimulated human peripheral lymphocytes by 50% were 1.5, 3.5, 4.0, 50, 150, and 150 ng/ml, respectively. The results suggested that the initial hydrolysis of T-2 toxin and the hydroxyla...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.6.1523-1526.1985

    authors: Forsell JH,Kateley JR,Yoshizawa T,Pestka JJ

    更新日期:1985-06-01 00:00:00

  • Genetic dissection of tropodithietic acid biosynthesis by marine roseobacters.

    abstract::The symbiotic association between the roseobacter Silicibacter sp. strain TM1040 and the dinoflagellate Pfiesteria piscicida involves bacterial chemotaxis to dinoflagellate-produced dimethylsulfoniopropionate (DMSP), DMSP demethylation, and ultimately a biofilm on the surface of the host. Biofilm formation is coincide...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02339-07

    authors: Geng H,Bruhn JB,Nielsen KF,Gram L,Belas R

    更新日期:2008-03-01 00:00:00

  • Molecular characterization of Frankia microsymbionts from spore-positive and spore-negative nodules in a natural alder stand.

    abstract::Among the Frankia strains capable of establishing a nitrogen-fixing symbiosis with actinorrhizal plants, in planta sporangial formation is not universal and has led to the distinction between spore-positive (Sp+) and spore-negative (Sp-) nodules. Numerous Frankia strains have been isolated in pure culture from Sp+ nod...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.4.1335-1341.1994

    authors: Simonet P,Bosco M,Chapelon C,Moiroud A,Normand P

    更新日期:1994-04-01 00:00:00

  • Morphological diversity of ruminal bacteriophages from sheep and cattle.

    abstract::Large numbers of bacteriophages (2 x 10(7) to 1 x 10(8)/ml) were present in ruminal fluid from sheep and cattle. Twenty-six distinct types were identified and placed in three morphological groups; several phages possessed unusual structural features. The large numbers and diversity of phages observed indicates a possi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.6.1637-1641.1988

    authors: Klieve AV,Bauchop T

    更新日期:1988-06-01 00:00:00

  • Improvement of xylose uptake and ethanol production in recombinant Saccharomyces cerevisiae through an inverse metabolic engineering approach.

    abstract::We used an inverse metabolic engineering approach to identify gene targets for improved xylose assimilation in recombinant Saccharomyces cerevisiae. Specifically, we created a genomic fragment library from Pichia stipitis and introduced it into recombinant S. cerevisiae expressing XYL1 and XYL2. Through serial subcult...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.8249-8256.2005

    authors: Jin YS,Alper H,Yang YT,Stephanopoulos G

    更新日期:2005-12-01 00:00:00

  • Divergence of Genomic Sequences between Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris.

    abstract::Relatedness between Lactococcus lactis subsp. cremoris and L. lactis subsp. lactis was assessed by Southern hybridization analysis, with cloned chromosomal genes as probes. The results indicate that strains of the two subspecies form two distinct groups and that the DNA sequence divergence between L. lactis subsp. lac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.12.4045-4047.1992

    authors: Godon JJ,Delorme C,Ehrlich SD,Renault P

    更新日期:1992-12-01 00:00:00

  • Synthesis of [C]Cellobiose with Clostridium thermocellum Cellobiose Phosphorylase.

    abstract::Cellobiose labeled either at the reducing end (4-O-beta-d-glucopyranosyl-d-[U-C]glucopyranose) or at the nonreducing end (4-O-beta-d-[U-C]glucopyranosyl-d -glucopyranose) was synthesized with Clostridium thermocellum cellobiose phosphorylase at greater than 98% purity. The radioactive cellobioses were identical to aut...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.4.902-904.1986

    authors: Ng TK,Zeikus JG

    更新日期:1986-10-01 00:00:00

  • A Synechococcus PglnA::luxAB fusion for estimation of nitrogen bioavailability to freshwater cyanobacteria.

    abstract::In contrast to extensive studies of phosphorus, widely considered the main nutrient limiting phytoplankton biomass in freshwater ecosystems, there have been few studies on the role of nitrogen in controlling phytoplankton populations. This situation may be due partly to the complexity in estimating its utilization and...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.3.1465-1474.2003

    authors: Gillor O,Harush A,Hadas O,Post AF,Belkin S

    更新日期:2003-03-01 00:00:00

  • Immunolatex localization by scanning electron microscopy of intestinal bacteria from cockroaches.

    abstract::An immunolatex reagent was developed from antiserum to Fusobacterium varium, an obligate anaerobe isolated from the colon of the cockroach, Periplaneta americana L. A new technique that enabled the preparation of a highly efficient immunolatex conjugate was used to localize the bacterium with scanning electron microsc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.1.166-171.1978

    authors: Bracke JW,Markovetz AJ

    更新日期:1978-01-01 00:00:00

  • Evaluation of a wipe surface sample method for collection of Bacillus spores from nonporous surfaces.

    abstract::Polyester-rayon blend wipes were evaluated for efficiency of extraction and recovery of powdered Bacillus atrophaeus spores from stainless steel and painted wallboard surfaces. Method limits of detection were also estimated for both surfaces. The observed mean efficiency of polyester-rayon blend wipe recovery from sta...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01082-06

    authors: Brown GS,Betty RG,Brockmann JE,Lucero DA,Souza CA,Walsh KS,Boucher RM,Tezak M,Wilson MC,Rudolph T

    更新日期:2007-02-01 00:00:00

  • Difluoromethane, a new and improved inhibitor of methanotrophy

    abstract::Difluoromethane (HFC-32; DFM) is compared to acetylene and methyl fluoride as an inhibitor of methanotrophy in cultures and soils. DFM was found to be a reversible inhibitor of CH4 oxidation by Methylococcus capsulatus (Bath). Consumption of CH4 in soil was blocked by additions of low levels of DFM (0.03 kPa), and thi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.11.4357-4362.1998

    authors: Miller LG,Sasson C,Oremland RS

    更新日期:1998-11-01 00:00:00

  • Aerobic, Selenium-Utilizing Bacillus Isolated from Seeds of Astragalus crotalariae.

    abstract::Bacillus sp. strain SS, an aerobic, gram-positive sporeformer, was isolated from seeds of Astragalus crotalariae, a selenium-accumulating plant. This bacillus grew in a nutrient broth (containing beef extract and peptone) if the medium was supplemented with high concentrations of selenium. Concentrations of Na(2)SeO(3...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.3.737-743.1982

    authors: Lindblow-Kull C,Shrift A,Gherna RL

    更新日期:1982-09-01 00:00:00

  • Colonization and internalization of Salmonella enterica in tomato plants.

    abstract::The consumption of fresh tomatoes has been linked to numerous food-borne outbreaks involving various serovars of Salmonella enterica. Recent advances in our understanding of plant-microbe interactions have shown that human enteric pathogenic bacteria, including S. enterica, are adapted to survive in the plant environm...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03704-12

    authors: Zheng J,Allard S,Reynolds S,Millner P,Arce G,Blodgett RJ,Brown EW

    更新日期:2013-04-01 00:00:00

  • Agar overlay method to measure adherence of Staphylococcus epidermidis to four plastic surfaces.

    abstract::The comparative adherence of seven strains of Staphylococcus epidermidis to plastic surfaces was measured by an agar overlay technique in which adherent organisms were counted by their ability to form colonies under an agar overlay. The degree of adherence to plastics decreased in the order polystyrene, polyvinyl chlo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.5.1322-1324.1985

    authors: Mackenzie AM,Rivera-Calderon RL

    更新日期:1985-11-01 00:00:00

  • Effects of time and watershed characteristics on the concentration of Cryptosporidium oocysts in river water.

    abstract::Water samples were collected from four locations on two rivers in Washington State and analyzed by membrane filtration-immunofluorescence assay to establish Cryptosporidium oocyst concentrations. Sampling locations were selected to evaluate effects of watershed character, from pristine mountain to downstream agricultu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.10.2790-2795.1991

    authors: Hansen JS,Ongerth JE

    更新日期:1991-10-01 00:00:00

  • Production and characterization of antibodies against nivalenol tetraacetate.

    abstract::Antibody against nivalenol tetraacetate (tetra-Ac-NIV) was prepared by immunization of rabbits with triacetyl-15-pimelate-NIV conjugated to bovine serum albumin. By using tritiated tetra-Ac-NIV as the test ligand, antibody titers were demonstrated as early as 4 weeks after immunization. Useful antibody for radioimmuno...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.4.1026-1030.1991

    authors: Wang CR,Chu FS

    更新日期:1991-04-01 00:00:00

  • Critical evaluation of membrane supports for use in quantitative hybridizations.

    abstract::The quantification of 16S rRNA by oligonucleotide probe hybridization was investigated with MagnaGraph (Micron Separation, Inc. [MSI]), Magna Charge (MSI), Magna (MSI), Immobilon-N (Millipore Corporation), and Nytran (Schleicher & Schuell, Inc.) membranes as supports for nucleic acid immobilization. The levels of dete...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.1.300-303.1996

    authors: Raskin L,Capman WC,Kane MD,Rittmann BE,Stahl DA

    更新日期:1996-01-01 00:00:00

  • Quantification of yeast and bacterial gene transcripts in retail cheeses by reverse transcription-quantitative PCR.

    abstract::The cheese microbiota contributes to a large extent to the development of the typical color, flavor, and texture of the final product. Its composition is not well defined in most cases and varies from one cheese to another. The aim of the present study was to establish procedures for gene transcript quantification in ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02360-12

    authors: Monnet C,Straub C,Castellote J,Onesime D,Bonnarme P,Irlinger F

    更新日期:2013-01-01 00:00:00

  • Role of IncP-1β plasmids pWDL7::rfp and pNB8c in chloroaniline catabolism as determined by genomic and functional analyses.

    abstract::Broad-host-range catabolic plasmids play an important role in bacterial degradation of man-made compounds. To gain insight into the role of these plasmids in chloroaniline degradation, we determined the first complete nucleotide sequences of an IncP-1 chloroaniline degradation plasmid, pWDL7::rfp and its close relativ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07480-11

    authors: Król JE,Penrod JT,McCaslin H,Rogers LM,Yano H,Stancik AD,Dejonghe W,Brown CJ,Parales RE,Wuertz S,Top EM

    更新日期:2012-02-01 00:00:00

  • Optimization of airborne endotoxin exposure assessment: effects of filter type, transport conditions, extraction solutions, and storage of samples and extracts.

    abstract::Endotoxin exposure occurs in homes and occupational environments and is known to cause adverse health effects. In order to compare results from different studies and establish standards, airborne endotoxin exposures should be assessed using standardized methods. Although the European Committee for Standardization (CEN...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00851-07

    authors: Spaan S,Heederik DJ,Thorne PS,Wouters IM

    更新日期:2007-10-01 00:00:00

  • Mosquito larvicidal activity of transgenic Anabaena strain PCC 7120 expressing combinations of genes from Bacillus thuringiensis subsp. israelensis.

    abstract::Various combinations of the genes cryIVA (cry4A), cryIVD (cry11A), and p20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 by means of Escherichia coli-Anabaena shuttle vector pRL488p and were expressed under control of two tandem stro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.12.4971-4974.1997

    authors: Xiaoqiang W,Vennison SJ,Huirong L,Ben-Dov E,Zaritsky A,Boussiba S

    更新日期:1997-12-01 00:00:00

  • Reducing biogenic-amine-producing bacteria, decarboxylase activity, and biogenic amines in raw milk cheese by high-pressure treatments.

    abstract::Biogenic amines may reach concentrations of public health concern in some cheeses. To minimize biogenic amine buildup in raw milk cheese, high-pressure treatments of 400 or 600 MPa for 5 min were applied on days 21 and 35 of ripening. On day 60, counts of lactic acid bacteria, enterococci, and lactobacilli were 1 to 2...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03368-12

    authors: Calzada J,del Olmo A,Picón A,Gaya P,Nuñez M

    更新日期:2013-02-01 00:00:00

  • Aspergillus glaucus Aquaglyceroporin Gene glpF Confers High Osmosis Tolerance in Heterologous Organisms.

    abstract::Aquaglyceroporins (GlpFs) that transport glycerol along with water and other uncharged solutes are involved in osmoregulation in myriad species. Fungal species form a large group of eukaryotic organisms, and their GlpFs may be diverse, exhibiting various activities. However, few filamentous fungal GlpFs have been biol...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02127-15

    authors: Liu XD,Wei Y,Zhou XY,Pei X,Zhang SH

    更新日期:2015-10-01 00:00:00