Effects of time and watershed characteristics on the concentration of Cryptosporidium oocysts in river water.

abstract：:In order to explore the biochemical scope of ochratoxin A-producing penicillia, we screened 48 Penicillium verrucosum isolates for the production of secondary metabolites. Fungal metabolites were analyzed by high-pressure liquid or gas chromatography coupled to diode array detection or mass spectrometry. The following...

journal_title：Applied and environmental microbiology

authors： Larsen TO,Svendsen A,Smedsgaard J

更新日期：2001-08-01 00:00:00

abstract：:Genome analysis revealed the existence of a putative transcriptional regulatory system governing CO metabolism in Thermococcus onnurineus NA1, a carboxydotrophic hydrogenogenic archaeon. The regulatory system is composed of CorQ with a 4-vinyl reductase domain and CorR with a DNA-binding domain of the LysR-type transc...

journal_title：Applied and environmental microbiology

authors： Kim MS,Choi AR,Lee SH,Jung HC,Bae SS,Yang TJ,Jeon JH,Lim JK,Youn H,Kim TW,Lee HS,Kang SG

更新日期：2015-03-01 00:00:00

abstract：:Nitrate was formed from ammonium at pH 3.2 to 6.1 in suspensions of a naturally acid forest soil; the maximum rates of formation occurred at ca. pH 4 to 5. Nitrate was also formed from soil nitrogen in suspensions incubated at 50 degrees C. Autotrophic nitrifying bacteria could not be isolated from this soil. Enrichme...

journal_title：Applied and environmental microbiology

authors： Stroo HF,Klein TM,Alexander M

更新日期：1986-11-01 00:00:00

abstract：:Melanin is a pigment produced by organisms throughout all domains of life. Due to its unique physicochemical properties, biocompatibility, and biostability, there has been an increasing interest in the use of melanin for broad applications. In the vast majority of studies, melanin has been either chemically synthesize...

journal_title：Applied and environmental microbiology

authors： Wang Z,Tschirhart T,Schultzhaus Z,Kelly EE,Chen A,Oh E,Nag O,Glaser ER,Kim E,Lloyd PF,Charles PT,Li W,Leary D,Compton J,Phillips DA,Dhinojwala A,Payne GF,Vora GJ

更新日期：2020-02-18 00:00:00

abstract：:Brevibacterium linens M18, isolated from red smear cheese, produces a substance that inhibits the growth of Listeria spp. and several coryneform and other gram-positive bacteria. No gram-negative bacteria were inhibited. The substance is heat labile, sensitive to proteolytic enzymes, and stable between pH 3 and 12. Hi...

journal_title：Applied and environmental microbiology

authors： Valdés-Stauber N,Scherer S

更新日期：1994-10-01 00:00:00

abstract：:We developed a biphasic culture system consisting of 4 ml of brucella agar (BA) and 6 ml of brucella broth (BB) in 25-cm2 tissue culture flasks, which were incubated in air (BB/BAa) or in a gas mixture of 5% O2, 10% CO2, and 85% N2 (BB/BAg). These media were also used with a supplement consisting of ferrous sulfate, s...

journal_title：Applied and environmental microbiology

authors： Rollins DM,Coolbaugh JC,Walker RI,Weiss E

更新日期：1983-01-01 00:00:00

abstract：:This report demonstrates the applicability of a combination of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) and chemometrics for rapid and reliable identification of vegetative cells of the causative agent of anthrax, Bacillus anthracis. Bacillus cultures were prepared ...

journal_title：Applied and environmental microbiology

authors： Lasch P,Beyer W,Nattermann H,Stämmler M,Siegbrecht E,Grunow R,Naumann D

更新日期：2009-11-01 00:00:00

abstract：:This study determined the membrane fluidity of clostridial endospores during treatment with heat and pressure with nisin or reutericyclin. Heating (90°C) reduced laurdan (6-dodecanoyl-2-dimethylaminonaphthalene) general polarization, corresponding to membrane fluidization. Pressure (200 MPa) stabilized membrane order....

journal_title：Applied and environmental microbiology

authors： Hofstetter S,Winter R,McMullen LM,Gänzle MG

更新日期：2013-03-01 00:00:00

abstract：:We investigated previously unknown associations between bacterial endosymbionts and bat flies of the subfamily Nycterophiliinae (Diptera, Streblidae). Molecular analyses revealed a novel clade of Gammaproteobacteria in Nycterophilia bat flies. This clade was not closely related to Arsenophonus-like microbes found in i...

journal_title：Applied and environmental microbiology

authors： Morse SF,Dick CW,Patterson BD,Dittmar K

更新日期：2012-12-01 00:00:00

abstract：:Cyanuric acid is an industrial chemical produced during the biodegradation of s-triazine pesticides. The biodegradation of cyanuric acid has been elucidated using a single model system, Pseudomonas sp. strain ADP, in which cyanuric acid hydrolase (AtzD) opens the s-triazine ring and AtzEG deaminates the ring-opened pr...

journal_title：Applied and environmental microbiology

authors： Aukema KG,Tassoulas LJ,Robinson SL,Konopatski JF,Bygd MD,Wackett LP

更新日期：2020-01-07 00:00:00

abstract：:The biodegradation of tert-butylphenyl diphenyl phosphate (BPDP) was examined in microcosms containing sediment and water from five different ecosystems as part of our studies to elucidate the environmental fate of phosphate ester flame retardants. Biodegradation of [14C]BPDP was monitored in the environmental microco...

journal_title：Applied and environmental microbiology

authors： Heitkamp MA,Freeman JP,Cerniglia CE

更新日期：1986-02-01 00:00:00

abstract：:Biotin-labeled DNA probes prepared from whole plasmids (5.5 and 4.8 kilobases) of two lactobacillus strains (Lactobacillus delbrueckii 21 and Lactobacillus reuteri 100-23) were used to detect the homologous bacteria in microtome-cryostat-prepared sections of murine forestomach. The forestomach sections were incubated ...

journal_title：Applied and environmental microbiology

authors： Tannock GW

更新日期：1989-02-01 00:00:00

abstract：:During batch fermentation, the rate of ethanol production per milligram of cell protein is maximal for a brief period early in this process and declines progressively as ethanol accumulates in the surrounding broth. Our studies demonstrate that the removal of this accumulated ethanol does not immediately restore ferme...

journal_title：Applied and environmental microbiology

authors： Dombek KM,Ingram LO

更新日期：1987-06-01 00:00:00

abstract：:Gaeumannomyces graminis, the causative agent of take-all disease of wheat, barley, and oats, was detected in infected wheat seedlings by using the polymerase chain reaction to amplify Gaeumannomyces-specific DNA fragments. Nested primers and two rounds of amplification were used to amplify two fragments, approximately...

journal_title：Applied and environmental microbiology

authors： Schesser K,Luder A,Henson JM

更新日期：1991-02-01 00:00:00

abstract：:One hundred seventy-eight mycoplasma strains isolated from South African poultry flocks between 2003 and 2015 were identified by full-genome sequencing and phylogenetic analysis of the 16S rRNA gene and were classified as follows: Mycoplasma gallisepticum (25%), M. gallinarum (25%), M. gallinaceum, (23%), M. pullorum ...

journal_title：Applied and environmental microbiology

authors： Beylefeld A,Wambulawaye P,Bwala DG,Gouws JJ,Lukhele OM,Wandrag DBR,Abolnik C

更新日期：2018-10-17 00:00:00

abstract：:The diagnostically important surface antigen pre-S2 of hepatitis B virus was produced in large amounts in the periplasmic space of Escherichia coli. The DNA fragments (pre-S2) coding the pre-S2 antigen were tandemly duplicated or triplicated and ligated in the same reading frame to a fragment containing the promoter a...

journal_title：Applied and environmental microbiology

authors： Kadokura H,Yoda K,Imai M,Yamasaki M

更新日期：1990-09-01 00:00:00

abstract：:A small spirillum, designated 5175, was isolated from an anaerobic enrichment culture for Desulfuromonas in which the major medium constituents were acetate and elemental sulfur. The organisms grew only under anaerobic or microaerophilic conditions. Elemental sulfur was formed anaerobically in a malate-sulfide medium,...

journal_title：Applied and environmental microbiology

authors： Wolfe RS,Penning N

更新日期：1977-02-01 00:00:00

abstract：:Anaerobic ammonium-oxidizing (anammox) bacteria have been recognized as an important sink for fixed nitrogen and are detected in many natural environments. However, their presence in terrestrial ecosystems has long been overlooked, and their contribution to the nitrogen cycling in natural and agricultural soils is cur...

journal_title：Applied and environmental microbiology

authors： Hu BL,Rush D,van der Biezen E,Zheng P,van Mullekom M,Schouten S,Sinninghe Damsté JS,Smolders AJ,Jetten MS,Kartal B

更新日期：2011-02-01 00:00:00

abstract：:A 1.8-kb chromosomal DNA fragment of the mycoplasmalike organism (MLO) associated with apple proliferation was sequenced. Three putative open reading frames were observed on this fragment. The protein encoded by open reading frame 2 shows significant homologies with bacterial nitroreductases. From the nucleotide seque...

journal_title：Applied and environmental microbiology

authors： Jarausch W,Saillard C,Dosba F,Bové JM

更新日期：1994-08-01 00:00:00

abstract：:Freshwater lakes harbor complex microbial communities, but these ecosystems are often dominated by acI Actinobacteria Members of this cosmopolitan lineage are proposed to bolster heterotrophic growth using phototrophy because their genomes encode actino-opsins (actR). This model has been difficult to validate experime...

journal_title：Applied and environmental microbiology

authors： Dwulit-Smith JR,Hamilton JJ,Stevenson DM,He S,Oyserman BO,Moya-Flores F,Garcia SL,Amador-Noguez D,McMahon KD,Forest KT

更新日期：2018-11-30 00:00:00

abstract：:In Pseudomonas sp. strain M114, the outer membrane receptor for ferric pseudobactin M114 was shown to transport ferric pseudobactins B10 and A225, in addition to its own. The gene encoding this receptor, which was previously cloned on pCUP3, was localized by Tn5 mutagenesis to a region comprising >1.6 kb of M114 DNA. ...

journal_title：Applied and environmental microbiology

authors： Morris J,O'sullivan DJ,Koster M,Leong J,Weisbeek PJ,O'gara F

更新日期：1992-02-01 00:00:00

abstract：:Mean pH values in pooled samples of foregut, midgut, and hindgut from adult Melanoplus sanguinipes, which had been raised in the laboratory on barley shoots and wheat bran, were 5.15, 6.39, and 5.98, respectively. Homogenates of midgut/hindgut sections and frass (feces) yielded colony counts of bacteria by the spread ...

journal_title：Applied and environmental microbiology

authors： Mead LJ,Khachatourians GG,Jones GA

更新日期：1988-05-01 00:00:00

abstract：:The determination of bacterial densities in aquatic sediments generally requires that a dilution-mixing treatment be used before enumeration of organisms by the most-probable-number fermentation tube method can be done. Differential sediment and organism settling rates may, however, influence the distribution of the m...

journal_title：Applied and environmental microbiology

authors： Doyle JD,Tunnicliff B,Kramer RE,Brickler SK

更新日期：1984-10-01 00:00:00

abstract：:A novel serine carboxypeptidase (EC 3.4.16.1) was found in an Aspergillus oryzae fermentation broth and was purified to homogeneity. This enzyme has a molecular weight of ca. 67,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and its specific activity is 21 U/mg for carbobenzoxy (Z)-Al...

journal_title：Applied and environmental microbiology

authors： Blinkovsky AM,Byun T,Brown KM,Golightly EJ

更新日期：1999-08-01 00:00:00

abstract：:Previously isolated dissimilatory perchlorate-reducing bacteria (DPRB) have been primarily affiliated with the Betaproteobacteria. Enrichments from the cathodic chamber of a bioelectrical reactor (BER) inoculated from creek water in Berkeley, CA, yielded a novel organism most closely related to a previously described ...

journal_title：Applied and environmental microbiology

authors： Thrash JC,Ahmadi S,Torok T,Coates JD

更新日期：2010-07-01 00:00:00

abstract：:Five different bacterial extracellular polymer extraction methods and a combination of two of these methods were compared on cultures of activated sludge, synthetic activated sludge, and Klebsiella aerogenes. High-speed centrifugation was the most effective extraction method for the K. aerogenes culture, based on the ...

journal_title：Applied and environmental microbiology

authors： Brown MJ,Lester JN

更新日期：1980-08-01 00:00:00

abstract：:The genus of Marinobacter is one of the most ubiquitous in the global oceans and assumed to significantly impact various biogeochemical cycles. The genome structure and content of Marinobacter aquaeolei VT8 was analyzed and compared with those from other organisms with diverse adaptive strategies. Here, we report the ...

journal_title：Applied and environmental microbiology

authors： Singer E,Webb EA,Nelson WC,Heidelberg JF,Ivanova N,Pati A,Edwards KJ

更新日期：2011-04-01 00:00:00

abstract：:Relationships between the hyphae of Agaricus bisporus (Lang) Sing and bacteria from the mushroom bed casing layer were examined with a scanning electron microscope. Hyphae growing in the casing layer differed morphologically from compost-grown hyphae. Whereas the compost contained thin single hyphae surrounded by calc...

journal_title：Applied and environmental microbiology

authors： Masaphy S,Levanon D,Tchelet R,Henis Y

更新日期：1987-05-01 00:00:00

abstract：:The deep-sea hydrothermal vent shrimp Rimicaris exoculata largely depends on a dense epibiotic chemoautotrophic bacterial community within its enlarged cephalothoracic chamber. However, our understanding of shrimp-bacterium interactions is limited. In this report, we focused on the deltaproteobacterial epibiont of R. ...

journal_title：Applied and environmental microbiology

authors： Jiang L,Liu X,Dong C,Huang Z,Cambon-Bonavita MA,Alain K,Gu L,Wang S,Shao Z

更新日期：2020-04-01 00:00:00

abstract：:Escherichia coli is one of the most widely used host microorganisms for recombinant protein expression and metabolic engineering, but it cannot efficiently secrete recombinant proteins to extracellular space. Here, extracellular protein secretion was enhanced in E. coli by deleting two d,d-carboxypeptidase genes (dacA...

journal_title：Applied and environmental microbiology

authors： Yang H,Lu X,Hu J,Chen Y,Shen W,Liu L

更新日期：2018-10-01 00:00:00