In situ determination of Clostridium endospore membrane fluidity during pressure-assisted thermal processing in combination with nisin or reutericyclin.

abstract：:Suspension of adenovirus type 5 in 2.0 ml of cell culture fluid at 37 degrees C were subjected to smoke from four cigarettes over a 4-h period. The cigarettes were smoked in a normal manner, and the inhaled smoke was exhaled through glass tubing into the virus-containing fluid. The virus suspensions were then titrated...

journal_title：Applied and environmental microbiology

authors： Bardell D

更新日期：1978-11-01 00:00:00

abstract：:In two previous studies dealing with lactic acid bacteria (LAB) from modified-atmosphere-packaged (MAP) broiler products and a broiler processing plant, several isolates remained unidentified. According to 16S rRNA gene sequence analysis, 36 isolates were assigned to the genus Enterococcus. Numerical analysis of combi...

journal_title：Applied and environmental microbiology

authors： Rahkila R,Johansson P,Säde E,Björkroth J

更新日期：2011-02-01 00:00:00

abstract：:We have developed a program that can accurately analyze the dynamic properties of tethered bacterial cells. The program works especially well with cells that tend to give rise to unstable rotations, such as polar-flagellated bacteria. The program has two novel components. The first dynamically adjusts the center of th...

journal_title：Applied and environmental microbiology

authors： Qian C,Wong CC,Swarup S,Chiam KH

更新日期：2013-08-01 00:00:00

abstract：:Specific Saccharomyces cerevisiae strains were recently found to be capable of efficiently utilizing inulin, but genetic mechanisms of inulin hydrolysis in yeast remain unknown. Here we report functional characteristics of invertase SUC2 from strain JZ1C and demonstrate that SUC2 is the key enzyme responsible for inul...

journal_title：Applied and environmental microbiology

authors： Wang SA,Li FL

更新日期：2013-01-01 00:00:00

abstract：:A thermophilic strain (D2) identified as a Bacillus sp. was isolated from an aerobic digestor of swine waste after several months of operation at 55 degrees C. Aerobic thermophilic batch treatment of swine waste inoculated with strain D2 was studied in a 4-liter fixed-bed reactor. Stabilization of the waste was achiev...

journal_title：Applied and environmental microbiology

authors： Beaudet R,Gagnon C,Bisaillon JG,Ishaque M

更新日期：1990-04-01 00:00:00

abstract：:Vibrio cholerae O1 strain TSI-4 (El Tor, Ogawa) can shift to a rugose colony morphology from its normal translucent colony morphology in response to nutrient starvation. We have investigated differences between the rugose and translucent forms of V. cholerae O1 strain TSI-4. Electron microscopic examination of the rug...

journal_title：Applied and environmental microbiology

authors： Wai SN,Mizunoe Y,Takade A,Kawabata SI,Yoshida SI

更新日期：1998-10-01 00:00:00

abstract：:Although many Lactobacillus strains used as probiotics are believed to modulate host immune responses, the molecular natures of the components of such probiotic microorganisms directly involved in immune modulation process are largely unknown. We aimed to assess the function of polysaccharide moiety of the cell wall o...

journal_title：Applied and environmental microbiology

authors： Yasuda E,Serata M,Sako T

更新日期：2008-08-01 00:00:00

abstract：:Yarrowia lipolytica produces brown extracellular pigments that correlate with tyrosine catabolism. During tyrosine depletion, the yeast accumulated homogentisic acid, p-hydroxyphenylethanol, and p-hydroxyphenylacetic acid in the medium. Homogentisic acid accumulated under all aeration conditions tested, but its concen...

journal_title：Applied and environmental microbiology

authors： Carreira A,Ferreira LM,Loureiro V

更新日期：2001-08-01 00:00:00

abstract：:Chlamydiae are important pathogens of humans and animals but diagnosis of chlamydial infections is still hampered by inadequate detection methods. Fluorescence in situ hybridization (FISH) using rRNA-targeted oligonucleotide probes is widely used for the investigation of uncultured bacteria in complex microbial commun...

journal_title：Applied and environmental microbiology

authors： Poppert S,Essig A,Marre R,Wagner M,Horn M

更新日期：2002-08-01 00:00:00

abstract：:The production of microcystins (MC) from Microcystis aeruginosa UTEX 2388 was investigated in a P-limited continuous culture. MC (MC-LR, MC-RR, and MC-YR) from lyophilized M. aeruginosa were extracted with 5% acetic acid, purified by a Sep-Pak C(18) cartridge, and then analyzed by high-performance liquid chromatograph...

journal_title：Applied and environmental microbiology

authors： Oh HM,Lee SJ,Jang MH,Yoon BD

更新日期：2000-01-01 00:00:00

abstract：:Three cases of Mycobacterium avium complex-related lung disorders were associated with two poorly maintained spa pools by genotypic investigations. Inadequate disinfection of the two spas had reduced the load of environmental bacteria to less than 1 CFU/ml but allowed levels of M. avium complex of 4.3 x 10(4) and 4.5 ...

journal_title：Applied and environmental microbiology

authors： Lumb R,Stapledon R,Scroop A,Bond P,Cunliffe D,Goodwin A,Doyle R,Bastian I

更新日期：2004-08-01 00:00:00

abstract：:Microbial partners play important roles in the biology and ecology of animals. In insects, maternally transmitted symbionts are especially common and can have host effects ranging from reproductive manipulation to nutrient provisioning and defense against natural enemies. In this study, we report a genus-wide associat...

journal_title：Applied and environmental microbiology

authors： Ballinger MJ,Moore LD,Perlman SJ

更新日期：2018-01-31 00:00:00

abstract：:Acylation of epsilon amino groups of lysyl side chains is a widespread modification of proteins and small molecules in cells of all three domains of life. Recently, we showed that Bacillus subtilis and Bacillus anthracis encode the GCN5-related N-acetyltransferase (GNAT) SatA that can acetylate and inactivate streptot...

journal_title：Applied and environmental microbiology

authors： Burckhardt RM,Escalante-Semerena JC

更新日期：2019-03-06 00:00:00

abstract：:Microscale fluorescence measurements were performed in photosynthetic biofilms at a spatial resolution of 100 to 200 microm with a new fiber-optic fluorometer which allowed four different excitation and emission wavelengths and was configured for measuring phycobiliproteins, chlorophylls, and bacteriochlorophylls. We ...

journal_title：Applied and environmental microbiology

authors： Thar R,Kühl M,Holst G

更新日期：2001-06-01 00:00:00

abstract：:Microbial communities from a subseafloor sediment core from the southwestern Sea of Okhotsk were evaluated by performing both cultivation-dependent and cultivation-independent (molecular) analyses. The core, which extended 58.1 m below the seafloor, was composed of pelagic clays with several volcanic ash layers contai...

journal_title：Applied and environmental microbiology

authors： Inagaki F,Suzuki M,Takai K,Oida H,Sakamoto T,Aoki K,Nealson KH,Horikoshi K

更新日期：2003-12-01 00:00:00

abstract：:Cr(VI) (chromate) is a widespread environmental contaminant. Bacterial chromate reductases can convert soluble and toxic chromate to the insoluble and less toxic Cr(III). Bioremediation can therefore be effective in removing chromate from the environment, especially if the bacterial propensity for such removal is enha...

journal_title：Applied and environmental microbiology

authors： Park CH,Keyhan M,Wielinga B,Fendorf S,Matin A

更新日期：2000-05-01 00:00:00

abstract：:Gangliosides, sialic acid-containing glycosphingolipids, are membrane constituents of vertebrates and are known to have important roles in cellular differentiation, adhesion, and recognition. We report here the isolation of a bacterium capable of degrading gangliotetraose-series gangliosides and a new method for the p...

journal_title：Applied and environmental microbiology

authors： Sumida T,Sueyoshi N,Ito M

更新日期：2002-11-01 00:00:00

abstract：:Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were...

journal_title：Applied and environmental microbiology

authors： Torensma R,Visser MJ,Aarsman CJ,Poppelier MJ,van Beurden R,Fluit AC,Verhoef J

更新日期：1992-12-01 00:00:00

abstract：:Time series incubations were conducted to provide estimates for the size selectivities and rates of protistan grazing that may be occurring in a sandy, contaminated aquifer. The experiments involved four size classes of fluorescently labeled groundwater bacteria (FLB) and 2- to 3-microns-long nanoflagellates, primaril...

journal_title：Applied and environmental microbiology

authors： Kinner NE,Harvey RW,Blakeslee K,Novarino G,Meeker LD

更新日期：1998-02-01 00:00:00

abstract：:The cloned 135-kDa CryIC delta-endotoxin from Bacillus thuringiensis is a lepidopteran-active toxin, displaying high activity in vivo against Spodoptera litoralis and Spodoptera frugiperda larvae and in vitro against the S. frugiperda Sf9 cell line. Here, we report that the CryIC delta-endotoxin cloned from B. thuring...

journal_title：Applied and environmental microbiology

authors： Smith GP,Merrick JD,Bone EJ,Ellar DJ

更新日期：1996-02-01 00:00:00

abstract：:Systematic environmental surveillance for poliovirus circulation has been conducted in Egypt since 2000. The surveillance has revealed three independent importations of wild-type poliovirus. In addition, several vaccine-derived polioviruses have been detected in various locations in Egypt. In addition to acute flaccid...

journal_title：Applied and environmental microbiology

authors： Blomqvist S,El Bassioni L,El Maamoon Nasr EM,Paananen A,Kaijalainen S,Asghar H,de Gourville E,Roivainen M

更新日期：2012-08-01 00:00:00

abstract：:We applied HNPP (2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate) to direct in situ PCR for the routine detection of specific bacterial cells at the single-cell level. PCR was performed on glass slides with digoxigenin-labeled dUTP. The digoxigenin-labeled PCR products were detected with alkaline phosphatase-lab...

journal_title：Applied and environmental microbiology

authors： Tani K,Kurokawa K,Nasu M

更新日期：1998-04-01 00:00:00

abstract：:Eighteen strains of Agrobacterium tumefaciens isolated from crown galls were tested for agrocin production. Of six agrocin-producing strains, one (D286) produced a broad-host-range agrocin active against strains carrying nopaline, octopine, and agropine type Ti plasmids. Sensitivity to agrocin D286 was found to map in...

journal_title：Applied and environmental microbiology

authors： Hendson M,Askjaer L,Thomson JA,van Montagu M

更新日期：1983-05-01 00:00:00

abstract：:Bacteriophages are very abundant in the biosphere, and viral infection is believed to affect the activity and genetic diversity of bacterial communities in aquatic environments. Lysogenic conversion, for example, can improve host fitness and lead to phage-mediated horizontal gene transfer. However, little is known abo...

journal_title：Applied and environmental microbiology

authors： Ghosh D,Roy K,Williamson KE,White DC,Wommack KE,Sublette KL,Radosevich M

更新日期：2008-01-01 00:00:00

abstract：:To ascertain whether position 131 of a mesophilic protease, subtilisin BPN', is a potential critical site for cold adaptation as screened by evolutionary engineering (S. Taguchi, A. Ozaki, and H. Momose, Appl. Environ. Microbiol. 64:492-495, 1998), a full set of subtilisin BPN' mutants with mutations at position 131 w...

journal_title：Applied and environmental microbiology

authors： Taguchi S,Komada S,Momose H

更新日期：2000-04-01 00:00:00

abstract：:The variable-region genes of monoclonal antibody against Bacillus cereus spores were cloned from mouse hybridoma cells by reverse transcription-PCR. The heavy- and light-chain variable-region genes were connected by a 45-base linker DNA to allow folding of the fusion protein into a functional tertiary structure. For d...

journal_title：Applied and environmental microbiology

authors： Koo K,Foegeding PM,Swaisgood HE

更新日期：1998-07-01 00:00:00

abstract：:Laccase is a copper-containing polyphenol oxidase that has great potential in industrial and biotechnological applications. Previous research has suggested that fungal laccase may be involved in the defense against oxidative stress, but there is little direct evidence supporting this hypothesis, and the mechanism by w...

journal_title：Applied and environmental microbiology

authors： Yang Y,Fan F,Zhuo R,Ma F,Gong Y,Wan X,Jiang M,Zhang X

更新日期：2012-08-01 00:00:00

abstract：:A method was developed for extraction of DNA from Chroococcidiopsis that overcomes obstacles posed by bacterial contamination and the presence of a thick envelope surrounding the cyanobacterial cells. The method is based on the resistance of Chroococcidiopsis to lysozyme and consists of a lysozyme treatment followed b...

journal_title：Applied and environmental microbiology

authors： Billi D,Grilli Caiola M,Paolozzi L,Ghelardini P

更新日期：1998-10-01 00:00:00

abstract：:Enterococcus mundtii CUGF08, a lactic acid bacterium isolated from alfalfa sprouts, was found to produce mundticin L, a new class IIa bacteriocin that has a high level of inhibitory activity against the genus Listeria. The plasmid-associated operons containing genes for the mundticin L precursor, the ATP binding casse...

journal_title：Applied and environmental microbiology

authors： Feng G,Guron GK,Churey JJ,Worobo RW

更新日期：2009-09-01 00:00:00

abstract：:Bacteria, yeasts, and molds isolated from partially processed iceberg lettuce were taxonomically classified. The majority of bacterial isolates were gram-negative rods. Pseudomonas, Erwinia, and Serratia species were commonly found. Yeasts most frequently isolated from lettuce included members of the genera Candida, C...

journal_title：Applied and environmental microbiology

authors： Magnuson JA,King AD Jr,Török T

更新日期：1990-12-01 00:00:00