Development of a direct in situ PCR method for detection of specific bacteria in natural environments.

abstract：:The utilization of gels, which are used for fluid drilling of seeds, as carriers of Bradyrhizobium japonicum for soybean (Glycine max (L.) Merr.) inoculation was studied. Gels of various chemical composition (magnesium silicate, potassium acrylate-acrylamide, grafted starch, and hydroxyethyl cellulose) were used, alth...

journal_title：Applied and environmental microbiology

authors： Jawson MD,Franzluebbers AJ,Berg RK

更新日期：1989-03-01 00:00:00

abstract：:Identification and classification of Vibrio species have relied upon band pattern methods (e.g., amplified fragment length polymorphism) and DNA-DNA hybridization. However, data generated by these methods cannot be used to build an online electronic taxonomy. In order to overcome these limitations, we developed the fi...

journal_title：Applied and environmental microbiology

authors： Thompson FL,Gomez-Gil B,Vasconcelos AT,Sawabe T

更新日期：2007-07-01 00:00:00

abstract：:Legionella pneumophila proliferates in freshwater environments at temperatures ranging from 25 to 45°C. To investigate the preference of different sequence types (ST) for a specific temperature range, growth of L. pneumophila serogroup 1 (SG1) ST1 (environmental strains), ST47, and ST62 (disease-associated strains) wa...

journal_title：Applied and environmental microbiology

authors： van der Kooij D,Brouwer-Hanzens AJ,Veenendaal HR,Wullings BA

更新日期：2016-10-27 00:00:00

abstract：:In this report, we describe the identification of functions that promote genomic recombination of linear DNA introduced into Pseudomonas cells by electroporation. The genes encoding these functions were identified in Pseudomonas syringae pv. syringae B728a based on similarity to the lambda Red Exo/Beta and RecET prote...

journal_title：Applied and environmental microbiology

authors： Swingle B,Bao Z,Markel E,Chambers A,Cartinhour S

更新日期：2010-08-01 00:00:00

abstract：:Xenorhabdus nematophila engages in a mutualistic association with the nematode Steinernema carpocapsae. The nematode invades and traverses the gut of susceptible insects. X. nematophila is released in the insect blood (hemolymph), where it suppresses host immune responses and functions as a pathogen. X. nematophila pr...

journal_title：Applied and environmental microbiology

authors： Singh S,Reese JM,Casanova-Torres AM,Goodrich-Blair H,Forst S

更新日期：2014-07-01 00:00:00

abstract：:A structural gene which codes for an extracellular lipase (EC 3.1.1.3) in Aeromonas hydrophila H3, which was isolated from a female hospitalized patient, was cloned in Escherichia coli by using pBR322 as a vector. Lipase purified from both A. hydrophila culture supernatant and the periplasmic fluids of E. coli contain...

journal_title：Applied and environmental microbiology

authors： Anguita J,Rodríguez Aparicio LB,Naharro G

更新日期：1993-08-01 00:00:00

abstract：:Simulation of a heat process used in the terminal dry-heat decontamination of the Viking spacecraft is reported. Naturally occurring airborne bacterial spores were collected on Teflon ribbons in selected spacecraft assembly areas and subsequently subjected to dry heat. Thermal inactivation experiments were conducted a...

journal_title：Applied and environmental microbiology

authors： Puleo JR,Bergstrom SL,Peeler JT,Oxborrow GS

更新日期：1978-09-01 00:00:00

abstract：:The production of a suitable substrate for the cultivation of the common white button mushroom, Agaricus bisporus, is referred to as composting. High microbiological activity causes temperatures of the composting material to rise as high as 80 degrees C. At stacking, an optimal oxygen consumption rate of 140 mumol of ...

journal_title：Applied and environmental microbiology

authors： Derikx PJ,Op Den Camp HJ,van der Drift C,Van Griensven LJ,Vogels GD

更新日期：1990-10-01 00:00:00

abstract：:Zearalenone [6-(10-hydroxy-6-oxo-trans-1-undecenyl)beta-resorcyclic acid lactone] is a hormone produced by Fusarium spp. which regulates the sexual stage in F. roseum. 3H- and 14C-labeled zearalenone were found to bind preferentially to one of two peaks containing uncharacterized proteins obtained from the cytosol of ...

journal_title：Applied and environmental microbiology

authors： Inaba T,Mirocha CJ

更新日期：1979-01-01 00:00:00

abstract：:A vineyard isolate of the yeast Saccharomyces cerevisiae, UCD932, was identified as a strain producing little or no detectable hydrogen sulfide during wine fermentation. Genetic analysis revealed that this trait segregated as a single genetic determinant. The gene also conferred a white colony phenotype on BiGGY agar ...

journal_title：Applied and environmental microbiology

authors： Linderholm A,Dietzel K,Hirst M,Bisson LF

更新日期：2010-12-01 00:00:00

abstract：:A dense exopolysaccharide (EPS) matrix is crucial for cyanobacterial survival in terrestrial xeric environments, in which cyanobacteria undergo frequent expansion and shrinkage processes during environmental desiccation-rehydration cycles. However, it is unclear how terrestrial cyanobacteria coordinate the structural ...

journal_title：Applied and environmental microbiology

authors： Liu W,Cui L,Xu H,Zhu Z,Gao X

更新日期：2017-10-31 00:00:00

abstract：:Stromatolites, organosedimentary structures formed by microbial activity, are found throughout the geological record and are important markers of biological history. More conspicuous in the past, stromatolites occur today in a few shallow marine environments, including Hamelin Pool in Shark Bay, Western Australia. Ham...

journal_title：Applied and environmental microbiology

authors： Papineau D,Walker JJ,Mojzsis SJ,Pace NR

更新日期：2005-08-01 00:00:00

abstract：:The association between grass roots and Azospirillum brasilense Sp 7 was investigated by the Fahraeus slide technique, using nitrogen-free medium. Young inoculated roots of pearl millet and guinea grass produced more mucilaginous sheath (mucigel), root hairs, and lateral roots than did uninoculated sterile controls. T...

journal_title：Applied and environmental microbiology

authors： Umali-Garcia M,Hubbell DH,Gaskins MH,Dazzo FB

更新日期：1980-01-01 00:00:00

abstract：:Several microorganisms were examined for their abilities to convert S-nicotine into nornicotine. Five microorganisms including Microsporum gypseum (ATCC 11395) produced nornicotine and three unknown metabolites. M. gypseum efficiently reduced nicotine-1'-N-oxide to nicotine, but no nornicotine was obtained when the N-...

journal_title：Applied and environmental microbiology

authors： Sindelar RD,Rosazza JP,Barfknecht CF

更新日期：1979-11-01 00:00:00

abstract：:A novel death-specific gene, ScDSP, was obtained from a death stage subtraction cDNA library of the diatom Skeletonema costatum. The full length of ScDSP cDNA was 921 bp in length, containing a 699-bp open reading frame encoding 232 amino acids and two stretches of 66 and 156 bp in the 5' and 3' untranslated regions, ...

journal_title：Applied and environmental microbiology

authors： Chung CC,Hwang SP,Chang J

更新日期：2005-12-01 00:00:00

abstract：:An organism capable of growth on pyridine was isolated from soil by enrichment culture techniques and identified as Micrococcus luteus. The organism oxidized pyridine for energy and released N contained in the pyridine ring as ammonium. The organism could not grow on mono- or disubstituted pyridinecarboxylic acids or ...

journal_title：Applied and environmental microbiology

authors： Sims GK,Sommers LE,Konopka A

更新日期：1986-05-01 00:00:00

abstract：:The possible origin of beef contamination and genetic diversity of Escherichia coli populations in beef cattle, on carcasses and ground beef, was examined by using random amplification of polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the fliC gene. E. coli was recovered...

journal_title：Applied and environmental microbiology

authors： Aslam M,Nattress F,Greer G,Yost C,Gill C,McMullen L

更新日期：2003-05-01 00:00:00

abstract：:An amylolytic Lactobacillus plantarum silage strain with the starch-degrading ability displayed by Lactobacillus amylovorus was developed. An active fragment of the gene coding for alpha-amylase production in L. amylovorus was cloned and integrated into the chromosome of the competitive inoculant strain L. plantarum L...

journal_title：Applied and environmental microbiology

authors： Fitzsimons A,Hols P,Jore J,Leer RJ,O'Connell M,Delcour J

更新日期：1994-10-01 00:00:00

abstract：:Blooms of the cyanobacterium Anabaena circinalis are a major worldwide problem due to their production of a range of toxins, in particular the neurotoxins anatoxin-a and paralytic shellfish poisons (PSPs). Although there is a worldwide distribution of A. circinalis, there is a geographical segregation of neurotoxin pr...

journal_title：Applied and environmental microbiology

authors： Beltran EC,Neilan BA

更新日期：2000-10-01 00:00:00

abstract：:A PCR-based method and a reverse transcriptase PCR (RT-PCR)-based method were developed for the detection of pathogenic bacteria in organic waste, using Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and Staphylococcus aureus as model organisms. In seeded organic waste samples, detection limits of l...

journal_title：Applied and environmental microbiology

authors： Burtscher C,Wuertz S

更新日期：2003-08-01 00:00:00

abstract：:In order to understand the role of the mar locus in Salmonella with regard to multiple antibiotic resistance, cyclohexane resistance, and outer membrane protein F (OmpF) regulation, a marA::gfp reporter mutant was constructed in an antibiotic-sensitive Salmonella enterica serovar Typhimurium DT104 background. Salicyla...

journal_title：Applied and environmental microbiology

authors： Randall LP,Woodward MJ

更新日期：2001-03-01 00:00:00

abstract：:Trichoderma reesei can produce up to 100 g/liter of extracellular proteins. The major and industrially relevant products are cellobiohydrolase I (CBHI) and the hemicellulase XYNI. The genes encoding both enzymes are transcriptionally activated by the regulatory protein Xyr1. The first 850 nucleotides of the cbh1 promo...

journal_title：Applied and environmental microbiology

authors： Kiesenhofer DP,Mach RL,Mach-Aigner AR

更新日期：2017-12-15 00:00:00

abstract：:During malolactic fermentation in wine by Oenococcus oeni, the degradation of citric acid was delayed compared to the degradation of malic acid. The maximum concentration of diacetyl, an intermediary compound in the citric acid metabolism with a buttery or nutty flavor, coincided with the exhaustion of malic acid in t...

journal_title：Applied and environmental microbiology

authors： Nielsen JC,Richelieu M

更新日期：1999-02-01 00:00:00

abstract：:Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not fully understood. To address this lack of information, a study of the epidemiology and ecology of V. cholerae O1 and O139...

journal_title：Applied and environmental microbiology

authors： Alam M,Hasan NA,Sadique A,Bhuiyan NA,Ahmed KU,Nusrin S,Nair GB,Siddique AK,Sack RB,Sack DA,Huq A,Colwell RR

更新日期：2006-06-01 00:00:00

abstract：:From necrotic tissue of a Nashi pear tree, 24 Erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. Thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. The defective gene hrpL was complemented with...

journal_title：Applied and environmental microbiology

authors： Jock S,Kim WS,Barny MA,Geider K

更新日期：2003-01-01 00:00:00

abstract：:Both Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis produce mosquitocidal toxins during sporulation and are extensively used in the field for control of mosquito populations. All the known toxins of the latter organism are known to be encoded on a large plasmid, pBtoxis. In an attempt to combine the...

journal_title：Applied and environmental microbiology

authors： Gammon K,Jones GW,Hope SJ,de Oliveira CM,Regis L,Silva Filha MH,Dancer BN,Berry C

更新日期：2006-03-01 00:00:00

abstract：:Two Acanthamoeba species, fed at three temperatures, expelled vesicles containing living Legionella pneumophila cells. Vesicles ranged from 2.1 to 6.4 microns in diameter and theoretically could contain several hundred bacteria. Viable L. pneumophila cells were observed within vesicles which had been exposed to two co...

journal_title：Applied and environmental microbiology

authors： Berk SG,Ting RS,Turner GW,Ashburn RJ

更新日期：1998-01-01 00:00:00

abstract：:Although the molecular surveillance network RotaNet-Italy provides useful nationwide data on rotaviruses causing severe acute gastroenteritis in children in Italy, scarce information is available on rotavirus circulation in the general Italian population, including adults with mild or asymptomatic infection. We invest...

journal_title：Applied and environmental microbiology

authors： Ruggeri FM,Bonomo P,Ianiro G,Battistone A,Delogu R,Germinario C,Chironna M,Triassi M,Campagnuolo R,Cicala A,Giammanco GM,Castiglia P,Serra C,Gaggioli A,Fiore L

更新日期：2015-01-01 00:00:00

abstract：:DNA microarray analysis showed that yfiD, yggB, and yggE genes were up-regulated when superoxide dismutase (SOD)-deficient Escherichia coli IM303 (I4) was cultivated under the oxidative stress generated by photoexcited TiO(2), and pYFD, pYGB, and pYGE were constructed by inserting the respective genes into a pUC 19 ve...

journal_title：Applied and environmental microbiology

authors： Kim SY,Nishioka M,Hayashi S,Honda H,Kobayashi T,Taya M

更新日期：2005-05-01 00:00:00

abstract：:The differential resistance of substratum-attached, detached, and planktonic cells of Salmonella enteritidis phage type 4 was studied by using several inimical processes and in vivo bioluminescence as a nondestructive, real-time reporter of metabolic activity. Bioluminescence in this strain was mediated by a construct...

journal_title：Applied and environmental microbiology

authors： Dhir VK,Dodd CE

更新日期：1995-05-01 00:00:00