Fiber-optic fluorometer for microscale mapping of photosynthetic pigments in microbial communities.

abstract：:A pathway was metabolically engineered to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biodegradable thermoplastic with proven commercial applications, from a single, unrelated carbon source. An expression system was developed in which a prpC strain of Salmonella enterica serovar Typhimurium, with a ...

journal_title：Applied and environmental microbiology

authors： Aldor IS,Kim SW,Prather KL,Keasling JD

更新日期：2002-08-01 00:00:00

abstract：:While some species in the Bacillus cereus group are well-characterized human pathogens (e.g., B. anthracis and B. cereus sensu stricto), the pathogenicity of other species (e.g., B. pseudomycoides) either has not been characterized or is presently not well understood. To provide an updated characterization of the path...

journal_title：Applied and environmental microbiology

authors： Miller RA,Jian J,Beno SM,Wiedmann M,Kovac J

更新日期：2018-03-01 00:00:00

abstract：:Cultures of Anabaena variabilis were exposed to different light intensities, and the time course of photoadaptation was measured by photosynthetic rate and changes in pigmentation. A shift down in intensity of 284 muEin . m . sec caused a temporary decrease in the photosynthetic response followed by gradual adaptation...

journal_title：Applied and environmental microbiology

authors： Collins CD,Boylen CW

更新日期：1982-07-01 00:00:00

abstract：:Malonyl-coenzyme A (CoA) decarboxylase, malonyl-CoA synthetase, and malonate transporter mutants of Rhizobium leguminosarum bv. viciae and trifolii fixed N2 at wild-type rates on pea and clover, respectively. Thus, malonate does not drive N2 fixation in legume nodules. ...

journal_title：Applied and environmental microbiology

authors： Karunakaran R,East AK,Poole PS

更新日期：2013-07-01 00:00:00

abstract：:Trichloroethylene (TCE)-transforming aquifer methanotrophs were evaluated for the influence of TCE oxidation toxicity and the effect of reductant availability on TCE transformation rates during methane starvation. TCE oxidation at relatively low (6 mg liter-1) TCE concentrations significantly reduced subsequent methan...

journal_title：Applied and environmental microbiology

authors： Henry SM,Grbić-Galić D

更新日期：1991-01-01 00:00:00

abstract：:A Vibrio parahaemolyticus strain isolated from the rhizosphere of the ecosystem dominant estuarine grass, Spartina alterniflora, was characterized and shown to carry nifH, the gene encoding the nitrogenase iron protein, and to fix N(2). Nitrogen fixation may contribute substantially to the adaptability, niche breadth,...

journal_title：Applied and environmental microbiology

authors： Criminger JD,Hazen TH,Sobecky PA,Lovell CR

更新日期：2007-09-01 00:00:00

abstract：:A computer program was developed to identify anaerobic bacteria by using simultaneous pattern recognition via a Bayesian probabilistic model. The system is intended for use as a rapid, precise, and reproducible aid in the identification of unknown isolates. The program operates on a data base of 28 genera comprising 2...

journal_title：Applied and environmental microbiology

authors： Kelley RW,Kellogg ST

更新日期：1978-03-01 00:00:00

abstract：:Bacterial source tracking is used to apportion fecal pollution among putative sources. Within this context, library-independent markers are genetic or phenotypic traits that can be used to identify the host origin without a need for library-dependent classification functions. The objective of this project was to use m...

journal_title：Applied and environmental microbiology

authors： Soule M,Kuhn E,Loge F,Gay J,Call DR

更新日期：2006-03-01 00:00:00

abstract：:Two strains of Penicillium digitatum and one strain of Penicillium italicum were exposed to various levels of sorbic acid and potassium sorbate, and the MICs were determined. Selected strains of the molds were then repeatedly exposed to subinhibitory levels of the compounds to determine whether increased tolerance mig...

journal_title：Applied and environmental microbiology

authors： Schroeder LL,Bullerman LB

更新日期：1985-10-01 00:00:00

abstract：:The effect of cattle manure and slurry application on percolation and survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium was investigated for different soil depths after the addition of water. Four treatments were chosen for the first set of experiments: (i) addition of inoculated farmyar...

journal_title：Applied and environmental microbiology

authors： Semenov AV,van Overbeek L,van Bruggen AH

更新日期：2009-05-01 00:00:00

abstract：:Pseudomonas oleovorans grows on C(6) to C(12)n-alkanes and 1-alkenes. These substrates are oxidized to the corresponding fatty acids, which are oxidized further via the beta-oxidation pathway, yielding shorter fatty acids which have lost one or more C(2) units. P. oleovorans normally utilizes beta-oxidation pathway in...

journal_title：Applied and environmental microbiology

authors： Lageveen RG,Huisman GW,Preusting H,Ketelaar P,Eggink G,Witholt B

更新日期：1988-12-01 00:00:00

abstract：:NisI confers immunity against nisin, with high substrate specificity to prevent a suicidal effect in nisin-producing Lactococcus lactis strains. However, the NisI maturation process as well as its influence on nisin resistance has not been characterized. Here, we report the roles of lipoprotein signal peptidase II (Ls...

journal_title：Applied and environmental microbiology

authors： Liu J,Xiong H,Du Y,Wiwatanaratanabutr I,Wu X,Zhao G,Zhu H,Caiyin Q,Qiao J

更新日期：2020-09-17 00:00:00

abstract：:We have assembled a collection of 13 psychrophilic ligA alleles that can serve as genetic elements for engineering mesophiles to a temperature-sensitive (TS) phenotype. When these ligA alleles were substituted into Francisella novicida, they conferred a TS phenotype with restrictive temperatures between 33 and 39°C. W...

journal_title：Applied and environmental microbiology

authors： Pankowski JA,Puckett SM,Nano FE

更新日期：2016-01-15 00:00:00

abstract：:This study was performed to determine the effects of feeding a fiber-rich fraction of Brassica vegetables on the immune response through changes in enteric bacteria and short-chain fatty acid (SCFA) production in normal mice. The boiled-water-insoluble fraction of Brassica rapa L. (nozawana), which consists mainly of ...

journal_title：Applied and environmental microbiology

authors： Tanaka S,Yamamoto K,Yamada K,Furuya K,Uyeno Y

更新日期：2016-04-18 00:00:00

abstract：:The contamination of waterways with fecal material is a persistent threat to public health. Identification of the sources of fecal contamination is a vital component for abatement strategies and for determination of total maximum daily loads. While phenotypic and genotypic techniques have been used to determine potent...

journal_title：Applied and environmental microbiology

authors： Hamilton MJ,Yan T,Sadowsky MJ

更新日期：2006-06-01 00:00:00

abstract：:A microbial community analysis using 16S rRNA gene sequencing was performed on borehole water and a granite rock core from Henderson Mine, a >1,000-meter-deep molybdenum mine near Empire, CO. Chemical analysis of borehole water at two separate depths (1,044 m and 1,004 m below the mine entrance) suggests that a sharp ...

journal_title：Applied and environmental microbiology

authors： Sahl JW,Schmidt R,Swanner ED,Mandernack KW,Templeton AS,Kieft TL,Smith RL,Sanford WE,Callaghan RL,Mitton JB,Spear JR

更新日期：2008-01-01 00:00:00

abstract：:The first DNA sequences obtained from arbuscular endomycorrhizal fungi are reported. They were obtained by directly sequencing overlapping amplified fragments of the nuclear genes coding for the small subunit rRNA. These sequences were used to develop a polymerase chain reaction primer (VANS1) that enables the specifi...

journal_title：Applied and environmental microbiology

authors： Simon L,Lalonde M,Bruns TD

更新日期：1992-01-01 00:00:00

abstract：:The ammonia-oxidizing prokaryote (AOP) community in three groundwater treatment plants and connected distribution systems was analyzed by quantitative real-time PCR and sequence analysis targeting the amoA gene of ammonia-oxidizing bacteria (AOB) and archaea (AOA). Results demonstrated that AOB and AOA numbers increas...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Voost S,van der Kooij D

更新日期：2009-07-01 00:00:00

abstract：:In studies designed to determine the factors responsible for control of herpesvirus replicaton in an infected cell, we examined the interaction of varicella-zoster (VZ) virus-infected human embryonic lung cells with the pesticide carbaryl. The replication of the cell-associated VZ virus was enhanced 2- to 13-fold as c...

journal_title：Applied and environmental microbiology

authors： Abrahamsen LH,Jerkofsky M

更新日期：1981-03-01 00:00:00

abstract：:The growth characteristics and acetate production of several Escherichia coli strains were compared by using shake flasks, batch fermentations, and glucose-feedback-controlled fed-batch fermentations to assess the potential of each strain to grow at high cell densities. Of the E. coli strains tested, including JM105, ...

journal_title：Applied and environmental microbiology

authors： Luli GW,Strohl WR

更新日期：1990-04-01 00:00:00

abstract：:Phomenoic acid and phomenolactone, previously isolated from the fungus Phoma lingam (Tode) Desm., have shown moderate antifungal and antibacterial properties in vitro. To rationalize the production of phomenoic acid, a kinetic study of its biosynthesis in the mycelium was performed. Phomenoic acid and phomenolactone a...

journal_title：Applied and environmental microbiology

authors： Topgi RS,Devys M,Bousquet JF,Kollmann A,Barbier M

更新日期：1987-05-01 00:00:00

abstract：:Sigma B (sigma(B)) is a stress-responsive alternative sigma factor that has been identified in various gram-positive bacteria. Seven different regulators of sigma B (Rsbs) are located in the sigB operons of both Bacillus subtilis and Listeria monocytogenes. In B. subtilis, these proteins contribute to regulation of si...

journal_title：Applied and environmental microbiology

authors： Chaturongakul S,Boor KJ

更新日期：2004-09-01 00:00:00

abstract：:High-throughput sequencing of the taxonomically informative 16S rRNA gene provides a powerful approach for exploring microbial diversity. Here we compare the performances of two common "benchtop" sequencing platforms, Illumina MiSeq and Ion Torrent Personal Genome Machine (PGM), for bacterial community profiling by 16...

journal_title：Applied and environmental microbiology

authors： Salipante SJ,Kawashima T,Rosenthal C,Hoogestraat DR,Cummings LA,Sengupta DJ,Harkins TT,Cookson BT,Hoffman NG

更新日期：2014-12-01 00:00:00

abstract：:β-Aminopeptidases have the unique capability to hydrolyze N-terminal β-amino acids, with varied preferences for the nature of β-amino acid side chains. This unique capability makes them useful as biocatalysts for synthesis of β-peptides and to kinetically resolve β-peptides and amides for the production of enantiopure...

journal_title：Applied and environmental microbiology

authors： John-White M,Gardiner J,Johanesen P,Lyras D,Dumsday G

更新日期：2019-07-18 00:00:00

abstract：:Various species of Absidia, Aspergillus, Cunninghamella, Trichothecium, Penicillium, and Phanerochaete were found to transform rotenone to one or more metabolites. Two biotransformation products were isolated from a preparative-scale incubation of rotenone with Cunninghamella blakesleeana and identified as 1',2'-dihyd...

journal_title：Applied and environmental microbiology

authors： Sariaslani FS,Rosazza JP

更新日期：1983-02-01 00:00:00

abstract：:The existence of a hydrogen sulfide:ferric ion oxidoreductase, which catalyzes the oxidation of elemental sulfur with ferric ions as an electron acceptor to produce ferrous and sulfite ions, was assayed with washed intact cells and cell extracts of various kinds of iron-oxidizing bacteria, such as Thiobacillus ferroox...

journal_title：Applied and environmental microbiology

authors： Sugio T,White KJ,Shute E,Choate D,Blake RC

更新日期：1992-01-01 00:00:00

abstract：:In this study, we describe the association of three Campylobacter jejuni metabolism-related traits, γ-glutamyl-transpeptidase (GGT), fucose permease (fucP), and secreted L-asparaginase [ansB(s)], with multilocus sequence types (STs). A total of 710 C. jejuni isolates with known STs were selected and originated from hu...

journal_title：Applied and environmental microbiology

authors： de Haan CP,Llarena AK,Revez J,Hänninen ML

更新日期：2012-08-01 00:00:00

abstract：:Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log10 of VLPs was depurated after 1...

journal_title：Applied and environmental microbiology

authors： Loisy F,Atmar RL,Le Saux JC,Cohen J,Caprais MP,Pommepuy M,Le Guyader FS

更新日期：2005-10-01 00:00:00

abstract：:Polyclonal antibodies of predetermined specificity for pediocin PA-1 (pedA1) have been generated by immunization of rabbits with a chemically synthesized C-terminal fragment of this bacteriocin (PH2) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). The sensitivity and specificity of the PH2-KLH-gener...

journal_title：Applied and environmental microbiology

authors： Martínez JM,Martínez MI,Suárez AM,Herranz C,Casaus P,Cintas LM,Rodríguez JM,Hernández PE

更新日期：1998-11-01 00:00:00

abstract：:Polyethylene glycol 6000 precipitation was found to be an effective concentration method that enhanced the chances for detecting human virus pathogens in environmental samples. Percent recoveries from eluates of fresh and estuarine waters with 8% polyethylene glycol 6000 averaged 86 for hepatitis A virus, 77 for human...

journal_title：Applied and environmental microbiology

authors： Lewis GD,Metcalf TG

更新日期：1988-08-01 00:00:00