Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots.

Abstract:

:The first DNA sequences obtained from arbuscular endomycorrhizal fungi are reported. They were obtained by directly sequencing overlapping amplified fragments of the nuclear genes coding for the small subunit rRNA. These sequences were used to develop a polymerase chain reaction primer (VANS1) that enables the specific amplification of a portion of the vesicular-arbuscular endomycorrhizal fungus small subunit rRNA directly from a mixture of plant and fungal tissues. The specificity of this primer for arbuscular endomycorrhizal fungi was demonstrated by testing it on a number of organisms and by sequencing the fragment amplified from colonized leek (Allium porum) roots. This approach, coupled with other molecular techniques, will facilitate rapid detection, identification, and possibly quantitation of arbuscular endomycorrhizal fungi.

journal_name

Appl Environ Microbiol

authors

Simon L,Lalonde M,Bruns TD

doi

10.1128/AEM.58.1.291-295.1992

keywords:

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

291-5

issue

1

eissn

0099-2240

issn

1098-5336

journal_volume

58

pub_type

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