Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots.


:The first DNA sequences obtained from arbuscular endomycorrhizal fungi are reported. They were obtained by directly sequencing overlapping amplified fragments of the nuclear genes coding for the small subunit rRNA. These sequences were used to develop a polymerase chain reaction primer (VANS1) that enables the specific amplification of a portion of the vesicular-arbuscular endomycorrhizal fungus small subunit rRNA directly from a mixture of plant and fungal tissues. The specificity of this primer for arbuscular endomycorrhizal fungi was demonstrated by testing it on a number of organisms and by sequencing the fragment amplified from colonized leek (Allium porum) roots. This approach, coupled with other molecular techniques, will facilitate rapid detection, identification, and possibly quantitation of arbuscular endomycorrhizal fungi.


Appl Environ Microbiol


Simon L,Lalonde M,Bruns TD





Has Abstract


1992-01-01 00:00:00












  • Role of phaD in accumulation of medium-chain-length Poly(3-hydroxyalkanoates) in Pseudomonas oleovorans.

    abstract::Pseudomonas oleovorans is capable of producing poly(3-hydroxyalkanoates) (PHAs) as intracellular storage material. To analyze the possible involvement of phaD in medium-chain-length (MCL) PHA biosynthesis, we generated a phaD knockout mutant by homologous recombination. Upon disruption of the phaD gene, MCL PHA polyme...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Klinke S,de Roo G,Witholt B,Kessler B

    更新日期:2000-09-01 00:00:00

  • Novel Rickettsiella bacterium in the leafhopper Orosius albicinctus (Hemiptera: Cicadellidae).

    abstract::Bacteria in the genus Rickettsiella (Coxiellaceae), which are mainly known as arthropod pathogens, are emerging as excellent models to study transitions between mutualism and pathogenicity. The current report characterizes a novel Rickettsiella found in the leafhopper Orosius albicinctus (Hemiptera: Cicadellidae), a m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Iasur-Kruh L,Weintraub PG,Mozes-Daube N,Robinson WE,Perlman SJ,Zchori-Fein E

    更新日期:2013-07-01 00:00:00

  • Kinetic concepts for measuring microbial rate constants: effects of nutrients on rate constants.

    abstract::We investigated the effect of preincubation of environmental waters amended with inorganic nutrients (nitrogen, phosphorus, and traces of iron and magnesium) on the kinetics of the microbial transformation of phenol, propanil, propyl ester of (2,4-dichlorophenoxy)acetic acid, methyl parathion, Ronnel, and methoxychlor...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Paris DF,Rogers JE

    更新日期:1986-02-01 00:00:00

  • Seasonal variation in lysogeny as depicted by prophage induction in Tampa Bay, Florida.

    abstract::A seasonal study of the distribution of lysogenic bacteria in Tampa Bay, Florida, was conducted over a 13-month period. Biweekly water samples were collected and either were left unaltered or had the viral population reduced by filtration (pore size, 0.2 micro m) and resuspension in filtered (pore size, 0.2 micro m) w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Williamson SJ,Houchin LA,McDaniel L,Paul JH

    更新日期:2002-09-01 00:00:00

  • Effect of storage time and temperature on the survival of Clostridium botulinum spores in acid media.

    abstract::Clostridium-botulinum type A and type B spores were stored in tomato juice (pH 4.2) and citric acid-phosphate buffer (pH 4.2) at 4, 22, and 32 degrees C for 180 days. The spore count was determined at different intervals over the 180-day storage period. There was no significant decrease in the number of type A spores ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Odlaug TE,Pflug IJ

    更新日期:1977-07-01 00:00:00

  • Competitiveness of Rhizobium trifolii Strains Associated with Red Clover (Trifolium pratense L.) in Mississippi Soils.

    abstract::Five strains of Rhizobium trifolii were evaluated in competition with indigenous populations in nodulating red clover (Trifolium pratense L.) cv. Kenland in two different soils in Mississippi. Double antibiotic resistance acquisition was used to measure the proportion of nodules occupied by the introduced mutant strai...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Materon LA,Hagedorn C

    更新日期:1982-11-01 00:00:00

  • Evidence for geographic isolation and signs of endemism within a protistan morphospecies.

    abstract::The possible existence of endemism among microorganisms resulting from and preserved by geographic isolation is one of the most controversial topics in microbial ecology. We isolated 31 strains of "Spumella-like" flagellates from remote sampling sites from all continents, including Antarctica. These and another 23 iso...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Boenigk J,Pfandl K,Garstecki T,Harms H,Novarino G,Chatzinotas A

    更新日期:2006-08-01 00:00:00

  • Stable-Isotope Analysis of a Combined Nitrification-Denitrification Sustained by Thermophilic Methanotrophs under Low-Oxygen Conditions.

    abstract::To simulate growth conditions experienced by microbiota at O(inf2)-limited interfaces of organic matter in compost, an experimental system capable of maintaining dual limitations of oxygen and carbon for extended periods, i.e., a pO(inf2)-auxostat, has been used. (sup15)N tracer studies on thermophilic (53(deg)C) deco...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Pel R,Oldenhuis R,Brand W,Vos A,Gottschal JC,Zwart KB

    更新日期:1997-02-01 00:00:00

  • Characterization and role of the branched-chain aminotransferase (BcaT) isolated from Lactococcus lactis subsp. cremoris NCDO 763.

    abstract::In Lactococcus lactis, which is widely used as a starter in the cheese industry, the first step of aromatic and branched-chain amino acid degradation is a transamination which is catalyzed by two major aminotransferases. We have previously purified and characterized biochemically and genetically the aromatic aminotran...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Yvon M,Chambellon E,Bolotin A,Roudot-Algaron F

    更新日期:2000-02-01 00:00:00

  • Different effects of transgenic maize and nontransgenic maize on nitrogen-transforming archaea and bacteria in tropical soils.

    abstract::The composition of the rhizosphere microbiome is a result of interactions between plant roots, soil, and environmental conditions. The impact of genetic variation in plant species on the composition of the root-associated microbiota remains poorly understood. This study assessed the abundances and structures of nitrog...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Cotta SR,Dias AC,Marriel IE,Andreote FD,Seldin L,van Elsas JD

    更新日期:2014-10-01 00:00:00

  • Cadmium Resistance Screening in Nitrilotriacetate-Buffered Minimal Media.

    abstract::Media used to determine the MICs of heavy metals for bacteria are unreliable because organic components in the media bind or chelate most of the metal being studied. To define specific metal activity in media and to maintain metal activity at a constant level, HEPES-MES [N-2-hydroxyethylpiperazine-N' -2-ethanesulfonic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Angle JS,Chaney RL

    更新日期:1989-08-01 00:00:00

  • Biogeography, evolution, and diversity of epibionts in phototrophic consortia.

    abstract::Motile phototrophic consortia are highly regular associations in which numerous cells of green sulfur bacteria surround a flagellated colorless beta-proteobacterium in the center. To date, seven different morphological types of such consortia have been described. In addition, two immotile associations involving green ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Glaeser J,Overmann J

    更新日期:2004-08-01 00:00:00

  • Conjugative transfer between Escherichia coli and Leptospira spp. as a new genetic tool.

    abstract::Our understanding of leptospiral pathogenesis, which remains poorly understood, depends on reliable genetic tools for functional analysis of genes in pathogenic strains. In this study, we report the first demonstration of conjugation between Escherichia coli and Leptospira spp. by using RP4 derivative conjugative plas...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Picardeau M

    更新日期:2008-01-01 00:00:00

  • Coupling Secretomics with Enzyme Activities To Compare the Temporal Processes of Wood Metabolism among White and Brown Rot Fungi.

    abstract::Wood-degrading fungi use a sequence of oxidative and hydrolytic mechanisms to loosen lignocellulose and then release and metabolize embedded sugars. These temporal sequences have recently been mapped at high resolution using directional growth on wood wafers, revealing previously obscured dynamics as fungi progressive...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Presley GN,Panisko E,Purvine SO,Schilling JS

    更新日期:2018-08-01 00:00:00

  • Genetic Diversity of Flavobacterium psychrophilum Isolates from Three Oncorhynchus spp. in the United States, as Revealed by Multilocus Sequence Typing.

    abstract:UNLABELLED:The use of a multilocus sequence typing (MLST) technique has identified the intraspecific genetic diversity of U.S. Flavobacterium psychrophilum, an important pathogen of salmonids worldwide. Prior to this analysis, little U.S. F. psychrophilum genetic information was known; this is of importance when consid...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Van Vliet D,Wiens GD,Loch TP,Nicolas P,Faisal M

    更新日期:2016-05-16 00:00:00

  • Development of quantitative real-time PCR assays for detection and quantification of surrogate biological warfare agents in building debris and leachate.

    abstract::Evaluation of the fate and transport of biological warfare (BW) agents in landfills requires the development of specific and sensitive detection assays. The objective of the current study was to develop and validate SYBR green quantitative real-time PCR (Q-PCR) assays for the specific detection and quantification of s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Saikaly PE,Barlaz MA,de Los Reyes FL 3rd

    更新日期:2007-10-01 00:00:00

  • Inhibition of biofilm formation by T7 bacteriophages producing quorum-quenching enzymes.

    abstract::Bacterial growth in biofilms is the major cause of recalcitrant biofouling in industrial processes and of persistent infections in clinical settings. The use of bacteriophage treatment to lyse bacteria in biofilms has attracted growing interest. In particular, many natural or engineered phages produce depolymerases to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Pei R,Lamas-Samanamud GR

    更新日期:2014-09-01 00:00:00

  • Efficient production of artificially designed gelatins with a Bacillus brevis system.

    abstract::Artificially designed gelatins comprising tandemly repeated 30-amino-acid peptide units derived from human alphaI collagen were successfully produced with a Bacillus brevis system. The DNA encoding the peptide unit was synthesized by taking into consideration the codon usage of the host cells, but no clones having a t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Kajino T,Takahashi H,Hirai M,Yamada Y

    更新日期:2000-01-01 00:00:00

  • Influence of cis Element Arrangement on Promoter Strength in Trichoderma reesei.

    abstract::Trichoderma reesei can produce up to 100 g/liter of extracellular proteins. The major and industrially relevant products are cellobiohydrolase I (CBHI) and the hemicellulase XYNI. The genes encoding both enzymes are transcriptionally activated by the regulatory protein Xyr1. The first 850 nucleotides of the cbh1 promo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Kiesenhofer DP,Mach RL,Mach-Aigner AR

    更新日期:2017-12-15 00:00:00

  • Identification and activities in situ of Nitrosospira and Nitrospira spp. as dominant populations in a nitrifying fluidized bed reactor.

    abstract::Bacterial aggregates from a chemolithoautotrophic, nitrifying fluidized bed reactor were investigated with microsensors and rRNA-based molecular techniques. The microprofiles of O2, NH4+, NO2-, and NO3- demonstrated the occurrence of complete nitrification in the outer 125 microgram of the aggregates. The ammonia oxid...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Schramm A,De Beer D,Wagner M,Amann R

    更新日期:1998-09-01 00:00:00

  • Transfer of Enteric Viruses Adenovirus and Coxsackievirus and Bacteriophage MS2 from Liquid to Human Skin.

    abstract::Indirect exposure to waterborne viruses increases the risk of infection, especially among children with frequent hand-to-mouth contacts. Here, we quantified the transfer of one bacteriophage (MS2) and two enteric viruses (adenovirus and coxsackievirus) from liquid to skin. MS2, a commonly used enteric virus surrogate,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Pitol AK,Bischel HN,Boehm AB,Kohn T,Julian TR

    更新日期:2018-10-30 00:00:00

  • Biogenic mineral production by a novel arsenic-metabolizing thermophilic bacterium from the Alvord Basin, Oregon.

    abstract::The Alvord Basin in southeast Oregon contains a variety of hydrothermal features which have never been microbiologically characterized. A sampling of Murky Pot (61 degrees C; pH 7.1) led to the isolation of a novel arsenic-metabolizing organism (YeAs) which produces an arsenic sulfide mineral known as beta-realgar, a ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Ledbetter RN,Connon SA,Neal AL,Dohnalkova A,Magnuson TS

    更新日期:2007-09-01 00:00:00

  • Comparison of bacterial extracellular polymer extraction methods.

    abstract::Five different bacterial extracellular polymer extraction methods and a combination of two of these methods were compared on cultures of activated sludge, synthetic activated sludge, and Klebsiella aerogenes. High-speed centrifugation was the most effective extraction method for the K. aerogenes culture, based on the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Brown MJ,Lester JN

    更新日期:1980-08-01 00:00:00

  • BadR and BadM Proteins Transcriptionally Regulate Two Operons Needed for Anaerobic Benzoate Degradation by Rhodopseudomonas palustris.

    abstract::The bacterium Rhodopseudomonas palustris grows with the aromatic acid benzoate and the alicyclic acid cyclohexanecarboxylate (CHC) as sole carbon sources. The enzymatic steps in an oxygen-independent pathway for CHC degradation have been elucidated, but it was unknown how the CHC operon (badHI aliAB badK) encoding the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Hirakawa H,Hirakawa Y,Greenberg EP,Harwood CS

    更新日期:2015-07-01 00:00:00

  • Rapid detection and identification of Legionella pneumophila by a membrane immunoassay.

    abstract::Legionella pneumophila was detected and identified by an immunoblot assay using a monoclonal antibody specific to serogroups 1 to 8. Samples containing L. pneumophila were plated on buffered charcoal yeast extract agar supplemented with glycine, vancomycin, and polymyxin B. After incubation at 35 degrees C for 3 days,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bérubé A,Trudel M,Payment P

    更新日期:1989-06-01 00:00:00

  • Oxidoreductive cellulose depolymerization by the enzymes cellobiose dehydrogenase and glycoside hydrolase 61.

    abstract::Several members of the glycoside hydrolase 61 (GH61) family of proteins have recently been shown to dramatically increase the breakdown of lignocellulosic biomass by microbial hydrolytic cellulases. However, purified GH61 proteins have neither demonstrable direct hydrolase activity on various polysaccharide or lignaci...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Langston JA,Shaghasi T,Abbate E,Xu F,Vlasenko E,Sweeney MD

    更新日期:2011-10-01 00:00:00

  • Microbiological comparison of surface soil and unsaturated subsurface soil from a semiarid high desert.

    abstract::Thirty-two chemoheterotrophic bacteria were isolated from unsaturated subsurface soil samples obtained from ca. 70 m below land surface in a high desert in southeastern Idaho. Most isolates were gram positive (84%) and strict aerobes (79%). Acridine orange direct counts of microbes in one subsurface sample showed lowe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Colwell FS

    更新日期:1989-09-01 00:00:00

  • Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR.

    abstract::Detection of pathogens in contaminated food products by PCR can result in false-positive data due to the amplification of DNA from nonviable cells. A new method based on reverse transcription-PCR (RT-PCR) amplification of mRNA for the specific detection of viable Listeria monocytogenes was developed. The expression of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Klein PG,Juneja VK

    更新日期:1997-11-01 00:00:00

  • Erosion and subsequent transport state of Escherichia coli from cowpats.

    abstract::Processes by which fecal bacteria enter overland flow and their transportation state to surface waters are poorly understood, making the effectiveness of measures designed to intercept this pathway, such as vegetated buffer strips, difficult to predict. Freshly made and aged (up to 30 days) cowpats were exposed to sim...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Muirhead RW,Collins RP,Bremer PJ

    更新日期:2005-06-01 00:00:00

  • Transformation of Rhodococcus fascians by High-Voltage Electroporation and Development of R. fascians Cloning Vectors.

    abstract::The analysis of the virulence determinants of phytopathogenic Rhodococcus fascians has been hampered by the lack of a system for introducing exogenous DNA. We investigated the possibility of genetic transformation of R. fascians by high-voltage electroporation of intact bacterial cells in the presence of plasmid DNA. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Desomer J,Dhaese P,Montagu MV

    更新日期:1990-09-01 00:00:00