Plasmid stability in immobilized and free recombinant Escherichia coli JM105(pKK223-200): importance of oxygen diffusion, growth rate, and plasmid copy number.

abstract：:The form of a previously developed Bĕlehrádek type of growth rate model was used to develop a probability model for defining the growth/no growth interface as a function of temperature (10 to 37 degrees C), pH (pH 2.8 to 6.9), lactic acid concentration (0 to 500 mM), and water activity (0.955 to 0.999; NaCl was used a...

journal_title：Applied and environmental microbiology

authors： Presser KA,Ross T,Ratkowsky DA

更新日期：1998-05-01 00:00:00

abstract：:The siderophore synthetic system in Shewanella species is able to synthesize dozens of macrocyclic siderophores in vitro with synthetic precursors. In vivo, however, although three siderophores are produced naturally in Shewanella algae B516, which carries a lysine decarboxylase (AvbA) specific for siderophore synthes...

journal_title：Applied and environmental microbiology

authors： Wang S,Liang H,Liu L,Jiang X,Wu S,Gao H

更新日期：2020-03-18 00:00:00

abstract：:Achromobacter spp. are nonfermentative Gram-negative bacilli considered emergent pathogens in cystic fibrosis (CF). Although some cross-transmission events between CF patients have been described, Achromobacter strains were mostly patient specific, suggesting sporadic acquisitions from nonhuman reservoirs. However, so...

journal_title：Applied and environmental microbiology

authors： Dupont C,Jumas-Bilak E,Doisy C,Aujoulat F,Chiron R,Marchandin H

更新日期：2018-11-15 00:00:00

abstract：:We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of the rapeseed BnACCg8 gene flanked by the hly-specific target seq...

journal_title：Applied and environmental microbiology

authors： Rodríguez-Lázaro D,Pla M,Scortti M,Monzó HJ,Vázquez-Boland JA

更新日期：2005-12-01 00:00:00

abstract：:This study determined the membrane fluidity of clostridial endospores during treatment with heat and pressure with nisin or reutericyclin. Heating (90°C) reduced laurdan (6-dodecanoyl-2-dimethylaminonaphthalene) general polarization, corresponding to membrane fluidization. Pressure (200 MPa) stabilized membrane order....

journal_title：Applied and environmental microbiology

authors： Hofstetter S,Winter R,McMullen LM,Gänzle MG

更新日期：2013-03-01 00:00:00

abstract：:Thiobacillus denitrificans is a widespread, chemolithoautotrophic bacterium with an unusual and environmentally relevant metabolic repertoire, which includes its ability to couple denitrification to sulfur compound oxidation; to catalyze anaerobic, nitrate-dependent oxidation of Fe(II) and U(IV); and to oxidize minera...

journal_title：Applied and environmental microbiology

authors： Letain TE,Kane SR,Legler TC,Salazar EP,Agron PG,Beller HR

更新日期：2007-05-01 00:00:00

abstract：:A major taxon of obligate marine bacteria within the order Actinomycetales has been discovered from ocean sediments. Populations of these bacteria (designated MAR 1) are persistent and widespread, spanning at least three distinct ocean systems. In this study, 212 actinomycete isolates possessing MAR 1 morphologies wer...

journal_title：Applied and environmental microbiology

authors： Mincer TJ,Jensen PR,Kauffman CA,Fenical W

更新日期：2002-10-01 00:00:00

abstract：:Clostridium acetobutylicum is not able to grow on glycerol as the sole carbon source since it cannot reoxidize the excess of NADH generated by glycerol catabolism. Nevertheless, when the pSPD5 plasmid, carrying the NADH-consuming 1,3-propanediol pathway from C. butyricum VPI 3266, was introduced into C. acetobutylicum...

journal_title：Applied and environmental microbiology

authors： González-Pajuelo M,Meynial-Salles I,Mendes F,Soucaille P,Vasconcelos I

更新日期：2006-01-01 00:00:00

abstract：:Most Shiga toxin-producing Escherichia coli (STEC) strains associated with severe disease, such as hemolytic-uremic syndrome (HUS), carry large enterohemolysin-encoding (ehxA) plasmids, e.g., pO157 and pO103, that contribute to STEC clinical manifestations. Six ehxA subtypes (A through F) exist that phylogenetically c...

journal_title：Applied and environmental microbiology

authors： Lorenz SC,Monday SR,Hoffmann M,Fischer M,Kase JA

更新日期：2016-10-14 00:00:00

abstract：:The nonlinear thermal inactivation of Aspergillus niger spores in phosphate-citrate buffer was studied. The thermal inactivation pattern of the spore consisted of a shoulder, an accelerated decline, and a tail at various constant temperatures around 60 degrees C. The pattern fitted a thermotolerant subpopulation model...

journal_title：Applied and environmental microbiology

authors： Fujikawa H,Itoh T

更新日期：1996-10-01 00:00:00

abstract：:Subsurface sediments were recovered from a 52-m-deep borehole cored in the 300 Area of the Hanford Site in southeastern Washington State to assess the potential for biogeochemical transformation of radionuclide contaminants. Microbial analyses were made on 17 sediment samples traversing multiple geological units: the ...

journal_title：Applied and environmental microbiology

authors： Lin X,Kennedy D,Peacock A,McKinley J,Resch CT,Fredrickson J,Konopka A

更新日期：2012-02-01 00:00:00

abstract：:Penicillium expansum isolated from meat and apples produced both patulin and citrinin. Toxin identity was confirmed by spectroscopic and physical methods. The mean lethal dose in chicken embryos was determined for toxins administered both singly and in various ratios. Data from simultaneous administration of mycotoxin...

journal_title：Applied and environmental microbiology

authors： Ciegler A,Vesonder RF,Jackson LK

更新日期：1977-04-01 00:00:00

abstract：:The detection of Listeria monocytogenes from food is currently carried out using a double enrichment. For the ISO methodology, this double enrichment is performed using half-Fraser and Fraser broths, in which the overgrowth of L. innocua can occur in samples where both species are present. In this study, we analyzed t...

journal_title：Applied and environmental microbiology

authors： Lemaître JP,Duroux A,Pimpie R,Duez JM,Milat ML

更新日期：2015-03-01 00:00:00

abstract：:The use of a PCR method to investigate the fate of liberated Q(beta) coliphage RNA in wastewater suggests that a positive PCR result indicates the recent presence of potentially viable virus in the sample and therefore increases the public health significance of the result. ...

journal_title：Applied and environmental microbiology

authors： Limsawat S,Ohgaki S

更新日期：1997-07-01 00:00:00

abstract：:The transposon Tn916 and self-mobilizing plasmid pAM beta 1 were conjugated from Enterococcus faecalis to the ruminal bacterium Streptococcus bovis JB1. Transconjugants were identified by resistance to tetracycline (Tn916) or erythromycin (pAM beta 1) and by Southern hybridization analyses. Transfer frequencies were 7...

journal_title：Applied and environmental microbiology

authors： Hespell RB,Whitehead TR

更新日期：1991-09-01 00:00:00

abstract：:The amino acid profiles, modified essential amino acid (MEAA) indexes, and in vitro pepsin digestibilities were determined for single-cell protein (SCP) from certain industrially important lactobacilli. For the three parameters examined, substantial differences were seen between different Lactobacillus species and bet...

journal_title：Applied and environmental microbiology

authors： Erdman MD,Bergen WG,Reddy CA

更新日期：1977-04-01 00:00:00

abstract：:In two previous studies dealing with lactic acid bacteria (LAB) from modified-atmosphere-packaged (MAP) broiler products and a broiler processing plant, several isolates remained unidentified. According to 16S rRNA gene sequence analysis, 36 isolates were assigned to the genus Enterococcus. Numerical analysis of combi...

journal_title：Applied and environmental microbiology

authors： Rahkila R,Johansson P,Säde E,Björkroth J

更新日期：2011-02-01 00:00:00

abstract：:Hyphomicrobium chloromethanicum CM2(T), an aerobic methylotrophic member of the alpha subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. H. chloromethanicum possesses an inducible enzyme system for utilization of chloromethane, in which two polypeptides (67-kDa CmuA...

journal_title：Applied and environmental microbiology

authors： McAnulla C,Woodall CA,McDonald IR,Studer A,Vuilleumier S,Leisinger T,Murrell JC

更新日期：2001-01-01 00:00:00

abstract：:Polyhydroxyalkanoates (PHAs) are microbial polyesters that can be used as completely biodegradable polymers, but the high production cost prevents their use in a wide range of applications. Recombinant Escherichia coli strains harboring the Ralstonia eutropha PHA biosynthesis genes have been reported to have several a...

journal_title：Applied and environmental microbiology

authors： Choi JI,Lee SY,Han K

更新日期：1998-12-01 00:00:00

abstract：:The production of the H(2)O(2)-generating enzyme pyranose oxidase (POD) (EC 1.1.3.10) (synonym, glucose 2-oxidase), two ligninolytic peroxidases, and laccase in wood decayed by three white rot fungi was investigated by correlated biochemical, immunological, and transmission electron microscopic techniques. Enzyme acti...

journal_title：Applied and environmental microbiology

authors： Daniel G,Volc J,Kubatova E

更新日期：1994-07-01 00:00:00

abstract：:Methylosinus trichosporium OB3b produces an extracellular copper-binding ligand (CBL) with high affinity for copper. Wild-type cells and mutants that express soluble methane monooxygenase (sMMO) in the presence and absence of copper (sMMOc) were used to obtain cell exudates that were separated and analyzed by size exc...

journal_title：Applied and environmental microbiology

authors： Téllez CM,Gaus KP,Graham DW,Arnold RG,Guzman RZ

更新日期：1998-03-01 00:00:00

abstract：:It has long been assumed that prolonged holding of environmental samples at the ambient air temperature prior to bacteriological analysis is detrimental to isolation and detection of Vibrio cholerae, the causative agent of pandemic cholera. The present study was aimed at understanding the effect of transporting enviro...

journal_title：Applied and environmental microbiology

authors： Alam M,Sadique A,Nur-A-Hasan,Bhuiyan NA,Nair GB,Siddique AK,Sack DA,Ahsan S,Huq A,Sack RB,Colwell RR

更新日期：2006-03-01 00:00:00

abstract：:Predicting the presence of enteric viruses in surface waters is a complex modeling problem. Multiple water quality parameters that indicate the presence of human fecal material, the load of fecal material, and the amount of time fecal material has been in the environment are needed. This paper presents the results of ...

journal_title：Applied and environmental microbiology

authors： Black LE,Brion GM,Freitas SJ

更新日期：2007-06-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed for the detection of whole cells of methanogens in samples from anaerobic continuously stirred tank digesters treating slurries of solid waste. The assay was found to allow for quantitative analysis of the most important groups of methanogens in samples from anaerobic...

journal_title：Applied and environmental microbiology

authors： Sørensen AH,Ahring BK

更新日期：1997-05-01 00:00:00

abstract：:Nonribosomal peptides (NRPs) are a class of secondary metabolites usually produced by microorganisms. They are of paramount importance in different applications, including biocontrol and pharmacy. Brevibacillus spp. are a rich source of NRPs yet have received little attention. In this study, we characterize four novel...

journal_title：Applied and environmental microbiology

authors： Li Z,de Vries RH,Chakraborty P,Song C,Zhao X,Scheffers DJ,Roelfes G,Kuipers OP

更新日期：2020-11-24 00:00:00

abstract：:Identification and classification of Vibrio species have relied upon band pattern methods (e.g., amplified fragment length polymorphism) and DNA-DNA hybridization. However, data generated by these methods cannot be used to build an online electronic taxonomy. In order to overcome these limitations, we developed the fi...

journal_title：Applied and environmental microbiology

authors： Thompson FL,Gomez-Gil B,Vasconcelos AT,Sawabe T

更新日期：2007-07-01 00:00:00

abstract：:A strain of the aerobic anoxygenic photosynthetic bacteria was isolated from a deep-ocean hydrothermal vent plume environment. The in vivo absorption spectra of cells indicate the presence of bacteriochlorophyll a incorporated into light-harvesting complex I and a reaction center. The general morphological and physiol...

journal_title：Applied and environmental microbiology

authors： Yurkov V,Beatty JT

更新日期：1998-01-01 00:00:00

abstract：:Dextransucrase (DSR-S) from Leuconostoc mesenteroides NRRL B-512F is a glucosyltransferase that catalyzes synthesis of soluble dextran from sucrose. In the presence of efficient acceptor molecules, such as maltose, the reaction pathway is shifted toward glucooligosaccharide synthesis. Like glucosyltransferases from or...

journal_title：Applied and environmental microbiology

authors： Monchois V,Reverte A,Remaud-Simeon M,Monsan P,Willemot RM

更新日期：1998-05-01 00:00:00

abstract：:The molecular epidemiology of 545 Salmonella enterica serovar Typhimurium isolates collected between 1977 and 2009 from cattle in Hokkaido, Japan, was investigated using pulsed-field gel electrophoresis (PFGE). Nine main clusters were identified from 116 PFGE patterns. Cluster I comprised 248 isolates, 243 of which po...

journal_title：Applied and environmental microbiology

authors： Tamamura Y,Uchida I,Tanaka K,Okazaki H,Tezuka S,Hanyu H,Kataoka N,Makino S,Kishima M,Kubota T,Kanno T,Hatama S,Ishihara R,Hata E,Yamada H,Nakaoka Y,Akiba M

更新日期：2011-03-01 00:00:00

abstract：:Pediocin PA-1 is an antimicrobial peptide (called bacteriocin) that shows inhibitory activity against the food-borne pathogen Listeria monocytogenes. To elucidate which residue(s) is responsible for this function, the antimicrobial activities of pediocin PA-1 mutants were evaluated and compared. Each of the 44 native ...

journal_title：Applied and environmental microbiology

authors： Tominaga T,Hatakeyama Y

更新日期：2006-02-01 00:00:00