Pyranose Oxidase, a Major Source of H(2)O(2) during Wood Degradation by Phanerochaete chrysosporium, Trametes versicolor, and Oudemansiella mucida.

Abstract:

:The production of the H(2)O(2)-generating enzyme pyranose oxidase (POD) (EC 1.1.3.10) (synonym, glucose 2-oxidase), two ligninolytic peroxidases, and laccase in wood decayed by three white rot fungi was investigated by correlated biochemical, immunological, and transmission electron microscopic techniques. Enzyme activities were assayed in extracts from decayed birch wood blocks obtained by a novel extraction procedure. With the coupled peroxidase-chromogen (3-dimethylaminobenzoic acid plus 3-methyl-2-benzothiazolinone hydrazone hydrochloride) spectrophotometric assay, the highest POD activities were detected in wood blocks degraded for 4 months and were for Phanerochaete chrysosporium (149 mU g [dry weight] of decayed wood), Trametes versicolor (45 mU g), and Oudemansiella mucida (1.2 mU g), corresponding to wood dry weight losses of 74, 58, and 13%, respectively. Mn-dependent peroxidase activities in the same extracts were comparable to those of POD, while lignin peroxidase activity was below the detection limit for all fungi with the veratryl alcohol assay. Laccase activity was high with T. versicolor (422 mU g after 4 months), in trace levels with O. mucida, and undetectable in P. chrysosporium extracts. Evidence for C-2 specificity of POD was shown by thin-layer chromatography detection of 2-keto-d-glucose as the reaction product. By transmission electron microscopy-immunocytochemistry, POD was found to be preferentially localized in the hyphal periplasmic space of P. chrysosporium and O. mucida and associated with membranous materials in hyphae growing within the cell lumina or cell walls of partially and highly degraded birch fibers. An extracellular distribution of POD associated with slime coating wood cell walls was also noted. The periplasmic distribution in hyphae and extracellular location of POD are consistent with the reported ultrastructural distribution of H(2)O(2)-dependent Mn-dependent peroxidases. This fact and the dominant presence of POD and Mn-dependent peroxidase in extracts from degraded wood suggest a cooperative role of the two enzymes during white rot decay by the test fungi.

journal_name

Appl Environ Microbiol

authors

Daniel G,Volc J,Kubatova E

doi

10.1128/AEM.60.7.2524-2532.1994

keywords:

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

2524-32

issue

7

eissn

0099-2240

issn

1098-5336

journal_volume

60

pub_type

杂志文章
  • Coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes.

    abstract::We reported recently a unique fungal structure, called the spiny ball, on the vegetative hyphae of Coprinus comatus (O. F. Müll.:Fr.) Pers. Although some observations regarding the role of this structure were presented, its function remained largely unknown. In this study, we showed that purified (isolated and washed)...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02770-06

    authors: Luo H,Liu Y,Fang L,Li X,Tang N,Zhang K

    更新日期:2007-06-01 00:00:00

  • Conjugal transfer of R-plasmid R1drd-19 in Escherichia coli below 22 degrees C.

    abstract::The conjugal transfer of R-plasmids is known to occur at temperatures above 22 degrees C. We found that R1drd-19 is transferable below 22 degrees C, and we discuss this finding in the context of plasmid transfer in environmental waters. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.42.5.789-791.1981

    authors: Singleton P,Anson AE

    更新日期:1981-11-01 00:00:00

  • New anaerobic, ammonium-oxidizing community enriched from peat soil.

    abstract::Anaerobic ammonium-oxidizing (anammox) bacteria have been recognized as an important sink for fixed nitrogen and are detected in many natural environments. However, their presence in terrestrial ecosystems has long been overlooked, and their contribution to the nitrogen cycling in natural and agricultural soils is cur...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02402-10

    authors: Hu BL,Rush D,van der Biezen E,Zheng P,van Mullekom M,Schouten S,Sinninghe Damsté JS,Smolders AJ,Jetten MS,Kartal B

    更新日期:2011-02-01 00:00:00

  • Effects of pesticides on cyanobacterium Plectonema boryanum and cyanophage LPP-1.

    abstract::Cyanobacterium Plectonema boryanum IU 594 and cyanophage LPP-1 were used as indicator organisms in a bioassay of 16 pesticides. Experiments such as spot tests, disk assays, growth curves, and one-step growth experiments were used to examine the effects of pesticides on the host and virus. Also, experiments were done i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.5.910-914.1984

    authors: Mallison SM 3rd,Cannon RE

    更新日期:1984-05-01 00:00:00

  • Metabolic behavior of Lactococcus lactis MG1363 in microaerobic continuous cultivation at a low dilution rate.

    abstract::Minute amounts of oxygen were supplied to a continuous cultivation of Lactococcus lactis subsp. cremoris MG1363 grown on a defined glucose-limited medium at a dilution rate of 0.1 h(-1). More than 80% of the carbon supplied with glucose ended up in fermentation products other than lactate. Addition of even minute amou...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.6.2677-2682.2001

    authors: Jensen NB,Melchiorsen CR,Jokumsen KV,Villadsen J

    更新日期:2001-06-01 00:00:00

  • Glutamate decarboxylase-mediated nisin resistance in Listeria monocytogenes.

    abstract::Analysis of a complete set of glutamate decarboxylase (gad) mutants of Listeria monocytogenes strain LO28 (ΔgadD1, ΔgadDT1, ΔgadD2, ΔgadT2, and ΔgadD3 mutants) revealed that the ΔgadD1 mutant is impaired in its ability to tolerate exposure to both sublethal and lethal levels of the lantibiotic nisin. gadD1 is strain v...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00203-10

    authors: Begley M,Cotter PD,Hill C,Ross RP

    更新日期:2010-10-01 00:00:00

  • Characterization and structural analysis of the laccase I gene from the newly isolated ligninolytic basidiomycete PM1 (CECT 2971).

    abstract::We have isolated and characterized the cDNA and genomic DNA coding for a phenoloxidase, laccase I, previously purified from culture supernatant of the newly isolated ligninolytic basidiomycete PM1 (CECT 2971). A cDNA library from basidiomycete PM1 was constructed, and laccase-encoding cDNAs were identified by screenin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.12.4129-4135.1993

    authors: Coll PM,Tabernero C,Santamaría R,Pérez P

    更新日期:1993-12-01 00:00:00

  • 19F nuclear magnetic resonance analysis of 5-fluorouracil metabolism in wild-type and 5-fluorouracil-resistant Nectria haematococca.

    abstract::A mutant (furA3) was isolated from the S1 wild-type strain of Nectria haematococca on the basis of its resistance to 5-fluorouracil (5FU). This mutant has greatly reduced activity of uracil phosphoribosyltransferase, a pyrimidine salvage enzyme catalyzing the synthesis of UMP from uracil. The metabolism of 5FU was exa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.10.2474-2479.1989

    authors: Parisot D,Malet-Martino MC,Crasnier P,Martino R

    更新日期:1989-10-01 00:00:00

  • Diversity and depth-specific distribution of SAR11 cluster rRNA genes from marine planktonic bacteria.

    abstract::Small-subunit (SSU) ribosomal DNA (rDNA) gene clusters are phylogenetically related sets of SSU rRNA genes, commonly encountered in genes amplified from natural populations. Genetic variability in gene clusters could result from artifacts (polymerase error or PCR chimera formation), microevolution (variation among rrn...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.1.63-70.1997

    authors: Field KG,Gordon D,Wright T,Rappé M,Urback E,Vergin K,Giovannoni SJ

    更新日期:1997-01-01 00:00:00

  • Effects of a Bacterial Hay Preservative (Pediococcus pentosaceus) on Hay under Experimental Storage Conditions.

    abstract::The abundant growth of molds and thermophilic actinomycetes in stored hay decreases its quality and can be hazardous for the producer who inhales these contaminants when the moldy hay is fed in closed barns. These microbes are responsible for a respiratory disease called farmer's lung. Products, including bacterial cu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.12.4240-4243.1995

    authors: Duchaine C,Lavoie MC,Cormier Y

    更新日期:1995-12-01 00:00:00

  • Cloning and nucleotide sequence of the gene coding for aspartokinase II from a thermophilic methylotrophic Bacillus sp.

    abstract::The structural gene coding for the lysine-sensitive aspartokinase II of the methylotrophic thermotolerant Bacillus sp. strain MGA3 was cloned from a genomic library by complementation of an Escherichia coli auxotrophic mutant lacking all three aspartokinase isozymes. The nucleotide sequence of the entire 2.2-kb PstI f...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.9.2806-2814.1992

    authors: Schendel FJ,Flickinger MC

    更新日期:1992-09-01 00:00:00

  • An alternative efficient procedure for purification of the obligate intracellular fish bacterial pathogen Piscirickettsia salmonis.

    abstract::Piscirickettsia salmonis is an obligate intracellular bacterial pathogen of salmonid fish and the etiological agent of the aggressive disease salmonid rickettsial syndrome. Today, this disease, also known as piscirickettsiosis, is the cause of high mortality in net pen-reared salmonids in southern Chile. Although the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.10.6268-6271.2003

    authors: Henríquez V,Rojas MV,Marshall SH

    更新日期:2003-10-01 00:00:00

  • 19F nuclear magnetic resonance as a tool to investigate microbial degradation of fluorophenols to fluorocatechols and fluoromuconates.

    abstract::A method was developed to study the biodegradation and oxidative biodehalogenation of fluorinated phenols by 19F nuclear magnetic resonance (NMR). Characterization of the 19F NMR spectra of metabolite profiles of a series of fluorophenols, converted by purified phenol hydroxylase, catechol 1,2-dioxygenase, and/or by t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.4.1256-1263.1998

    authors: Boersma MG,Dinarieva TY,Middelhoven WJ,van Berkel WJ,Doran J,Vervoort J,Rietjens IM

    更新日期:1998-04-01 00:00:00

  • Recovery of viruses from vegetable surfaces.

    abstract::The efficiency of a system developed for the recovery of viruses contaminating large quantities of vegetables was investigated in the laboratory and tested in the field. Viruses seeded onto a number of leafy vegetables in the laboratory were eluted with a phosphate-buffered saline solution (pH 9.0). The eluate was cla...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.6.1389-1394.1982

    authors: Ward BK,Chenoweth CM,Irving LG

    更新日期:1982-12-01 00:00:00

  • Mortality Caused by Bath Exposure of Zebrafish (Danio rerio) Larvae to Nervous Necrosis Virus Is Limited to the Fourth Day Postfertilization.

    abstract::Nervous necrosis virus (NNV) is a member of the Betanodavirus genus that causes fatal diseases in over 40 species of fish worldwide. Mortality among NNV-infected fish larvae is almost 100%. In order to elucidate the mechanisms responsible for the susceptibility of fish larvae to NNV, we exposed zebrafish larvae to NNV...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04175-14

    authors: Morick D,Faigenbaum O,Smirnov M,Fellig Y,Inbal A,Kotler M

    更新日期:2015-05-15 00:00:00

  • Mechanism of the synergistic inactivation of Escherichia coli by UV-C light at mild temperatures.

    abstract::UV light only penetrates liquid food surfaces to a very short depth, thereby limiting its industrial application in food pasteurization. One promising alternative is the combination of UV light with mild heat (UV-H), which has been demonstrated to produce a synergistic bactericidal effect. The aim of this article is t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00623-13

    authors: Gayán E,Mañas P,Álvarez I,Condón S

    更新日期:2013-07-01 00:00:00

  • Inactivation of shiga toxin-producing Escherichia coli (STEC) and degradation and removal of cellulose from STEC surfaces by using selected enzymatic and chemical treatments.

    abstract::Some Shiga toxin-producing Escherichia coli (STEC) strains produce extracellular cellulose, a long polymer of glucose with β-1-4 glycosidic bonds. This study evaluated the efficacies of selected enzymatic and chemical treatments in inactivating STEC and degrading/removing the cellulose on STEC surfaces. Six cellulose-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06450-11

    authors: Park YJ,Chen J

    更新日期:2011-12-01 00:00:00

  • Engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli.

    abstract::The cyanophycin (CGP) synthetase gene (cphANE1) of the transposon-induced argL mutant NE1 of the cyanobacterium Nostoc ellipsosporum, which exhibits a CGP-leaky phenotype during diazotrophical growth, was cloned and expressed in Escherichia coli strain TOP10. Its amino acid sequence exhibited high similarities to CphA...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01132-06

    authors: Hai T,Frey KM,Steinbüchel A

    更新日期:2006-12-01 00:00:00

  • Effect of UV light disinfection on antibiotic-resistant coliforms in wastewater effluents.

    abstract::Total coliforms and total coliforms resistant to streptomycin, tetracycline, or chloramphenicol were isolated from filtered activated sludge effluents before and after UV light irradiation. Although the UV irradiation effectively disinfected the wastewater effluent, the percentage of the total surviving coliform popul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.2.371-377.1982

    authors: Meckes MC

    更新日期:1982-02-01 00:00:00

  • Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum.

    abstract::Histoplasma capsulatum is very prevalent in the environment and is one of the most common causes of mycoses in humans and diverse animals in Brazil. Multiple typing methods have been developed to study H. capsulatum epidemiology; however, there is limited information concerning comparisons of results obtained with dif...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02004-09

    authors: Muniz Mde M,Morais E Silva Tavares P,Meyer W,Nosanchuk JD,Zancope-Oliveira RM

    更新日期:2010-07-01 00:00:00

  • Utilization of dissolved nitrogen by heterotrophic bacterioplankton: effect of substrate c/n ratio.

    abstract::The significance of dissolved combined amino acids (DCAA), dissolved free amino acids (DFAA), and dissolved DNA (D-DNA) as sources of C and N for marine bacteria in batch cultures with variable substrate C/N ratios was studied. Glucose, ammonium, alanine, and phosphate were added to the cultures to produce C/N ratios ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.11.4124-4133.1994

    authors: Jørgensen NO,Kroer N,Coffin RB

    更新日期:1994-11-01 00:00:00

  • Growth inhibition of various Enterobacteriaceae species by the yeast Hansenula anomala during storage of moist cereal grain.

    abstract::Eleven of 13 Enterobacteriaceae species tested grew in moist stored wheat, highlighting a potential risk of this energy-saving airtight storage method. When Hansenula anomala was coinoculated, all Enterobacteriaceae species were significantly inhibited after 2 months of storage, six of them to below the detection limi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06024-11

    authors: Olstorpe M,Schnürer J,Passoth V

    更新日期:2012-01-01 00:00:00

  • Binding of pediocin PA-1 with anionic lipid induces model membrane destabilization.

    abstract::To obtain molecular insights into the action mode of antimicrobial activity of pediocin PA-1, the interactions between this bacteriocin and dimyristoylphosphatidylcholine (DMPC) or dimyristoylphosphatidylglycerol (DMPG) model membranes have been investigated in D(2)O at pD 6 by Fourier transform infrared spectroscopy....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.11.6777-6784.2003

    authors: Gaussier H,Lefèvre T,Subirade M

    更新日期:2003-11-01 00:00:00

  • Saccharomyces cerevisiae genome shuffling through recursive population mating leads to improved tolerance to spent sulfite liquor.

    abstract::Spent sulfite liquor (SSL) is a waste effluent from sulfite pulping that contains monomeric sugars which can be fermented to ethanol. However, fermentative yeasts used for the fermentation of the sugars in SSL are adversely affected by the inhibitory substances in this complex feedstock. To overcome this limitation, e...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02769-10

    authors: Pinel D,D'Aoust F,del Cardayre SB,Bajwa PK,Lee H,Martin VJ

    更新日期:2011-07-01 00:00:00

  • Production of Poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution.

    abstract::Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pH-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid conta...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.8.3624-3627.2000

    authors: Ahn WS,Park SJ,Lee SY

    更新日期:2000-08-01 00:00:00

  • Loss of gibberellin production in Fusarium verticillioides (Gibberella fujikuroi MP-A) is due to a deletion in the gibberellic acid gene cluster.

    abstract::Fusarium verticillioides (Gibberella fujikuroi mating population A [MP-A]) is a widespread pathogen on maize and is well-known for producing fumonisins, mycotoxins that cause severe disease in animals and humans. The species is a member of the Gibberella fujikuroi species complex, which consists of at least 11 differe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01819-08

    authors: Bömke C,Rojas MC,Hedden P,Tudzynski B

    更新日期:2008-12-01 00:00:00

  • Occurrence of toxicity among protease, amylase, and color mutants of a nontoxic soy sauce koji mold.

    abstract::A soy sauce koji mold, Aspergillus flavus var. columnaris Raper and Fennel (ATCC 44310), was treated with UV irradiation to obtain mutant strains possessing high protease activities, high amylase activities, and light-colored conidia. Selected mutant strains were tested for toxicity, and some were found acutely toxic ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.8.1980-1982.1987

    authors: Kalayanamitr A,Bhumiratana A,Flegel TW,Glinsukon T,Shinmyo A

    更新日期:1987-08-01 00:00:00

  • Suitability of quantitative real-time PCR to estimate the biomass of fungal root endophytes.

    abstract::A nested single-copy locus-based quantitative PCR (qPCR) assay and a multicopy locus-based qPCR assay were developed to estimate endophytic biomass of fungal root symbionts belonging to the Phialocephala fortinii sensu lato-Acephala applanata species complex (PAC). Both assays were suitable for estimation of endophyti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00907-10

    authors: Tellenbach C,Grünig CR,Sieber TN

    更新日期:2010-09-01 00:00:00

  • A simple, efficient method for the separation of humic substances and DNA from environmental samples.

    abstract::Three different gels (Sepharose 4B, Sephadex G-200, and Sephadex G-50) were evaluated as a means of removing humic contaminants from DNA extracts of environmental samples. Sepharose 4B gave superior separation of DNA from humics, and DNA purified in this way showed consistently greater amplification than DNA purified ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.12.4993-4995.1997

    authors: Jackson CR,Harper JP,Willoughby D,Roden EE,Churchill PF

    更新日期:1997-12-01 00:00:00

  • Medium-chain fatty acids decrease colonization and invasion through hilA suppression shortly after infection of chickens with Salmonella enterica serovar Enteritidis.

    abstract::The most common source of Salmonella infections in humans is food of poultry origin. Salmonella enterica serovar Enteritidis has a particular affinity for the contamination of the egg supply. In this study, the medium-chain fatty acids (MCFA), caproic, caprylic, and capric acid, were evaluated for the control of Salmo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.6.3582-3587.2004

    authors: Van Immerseel F,De Buck J,Boyen F,Bohez L,Pasmans F,Volf J,Sevcik M,Rychlik I,Haesebrouck F,Ducatelle R

    更新日期:2004-06-01 00:00:00