Abstract:
:The growth characteristics and acetate production of several Escherichia coli strains were compared by using shake flasks, batch fermentations, and glucose-feedback-controlled fed-batch fermentations to assess the potential of each strain to grow at high cell densities. Of the E. coli strains tested, including JM105, B, W3110, W3100, HB101, DH1, CSH50, MC1060, JRG1046, and JRG1061, strains JM105 and B were found to have the greatest relative biomass accumulation, strain MC1060 accumulated the highest concentrations of acetic acid, and strain B had the highest growth rates under the conditions tested. In glucose-feedback-controlled fed-batch fermentations, strains B and JM105 produced only 2 g of acetate.liter-1 while accumulating up to 30 g of biomass.liter-1. Under identical conditions, strains HB101 and MC1060 accumulated less than 10 g of biomass.liter-1 and strain MC1060 produced 8 g of acetate.liter-1. The addition of various concentrations of sodium acetate to the growth medium resulted in a logarithmic decrease, with respect to acetate concentration, in the growth rates of E. coli JM105, JM105(pOS4201), and JRG1061. These data indicated that the growth of the E. coli strains was likely to be inhibited by the acetate they produced when grown on media containing glucose. A model for the inhibition of growth of E. coli by acetate was derived from these experiments to explain the inhibition of acetate on E. coli strains at neutral pH.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Luli GW,Strohl WRdoi
10.1128/AEM.56.4.1004-1011.1990subject
Has Abstractpub_date
1990-04-01 00:00:00pages
1004-11issue
4eissn
0099-2240issn
1098-5336journal_volume
56pub_type
杂志文章abstract::Mass spectral analysis of T-2 toxin formed during the growth of Fusarium sporotrichioides (ATCC 24043) in the presence of H(2)O showed incorporation of up to three O atoms per toxin molecule. The carbonyl oxygens of the acetates at C-4 and C-15 and of the isovalerate at C-8 were derived from H(2)O. Toxin formed in the...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.51.3.493-497.1986
更新日期:1986-03-01 00:00:00
abstract::The PCR technique has potential for use in detection of low concentrations of airborne microorganisms. In this study, the sensitivity of PCR and its susceptibility to environmental interference were assessed with Escherichia coli DH1 as the target organism. Air samples, containing environmental bioaerosols, were colle...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.10.3639-3644.1995
更新日期:1995-10-01 00:00:00
abstract::An extracellular serine protease produced by a mutant, M1, derived from Streptomyces albogriseolus S-3253 that no longer produces a protease inhibitor (Streptomyces subtilisin inhibitor [SSI]) was isolated. A 20-kDa protein was purified by its affinity for SSI and designated SAM-P20. The amino acid sequence of the ami...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.1.180-186.1995
更新日期:1995-01-01 00:00:00
abstract::Growth on 3-chlorobenzoate was found to induce the enzymes of the protocatechuate meta ring fission pathway in Alcaligenes sp. strain BR60. The chlorobenzoate catabolic genes, designated cba, were localized to a 3.7-kb NotI-EcoRI fragment within the nonrepeated region of the composite transposon Tn5271. The cba genes ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.11.3625-3633.1993
更新日期:1993-11-01 00:00:00
abstract::Two different cadA cadmium resistance determinants (cadA1, first identified in Tn5422, and cadA2, associated with pLM80) were detected among cadmium-resistant Listeria monocytogenes strains from turkey processing plants. Prevalence of cadA1 versus cadA2 was serotype associated. Cadmium-resistant isolates that were als...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01751-09
更新日期:2010-01-01 00:00:00
abstract::The competition between selected or commercial killer strains of type K2 and sensitive commercial strains of Saccharomyces cerevisiae was studied under various conditions in sterile grape juice fermentations. The focus of this study was the effect of yeast inoculation levels and the role of assimilable nitrogen nutrit...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.7.2821-2825.1997
更新日期:1997-07-01 00:00:00
abstract::Staphylococcus aureus is a gram-positive pathogen that causes a variety of diseases, including bovine mastitis, which has severe economic consequences. Standard antibiotic treatment results in selection of resistant strains, leading to a need for alternative treatments, such as bacteriophage therapy. Forty-nine S. aur...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02641-08
更新日期:2009-07-01 00:00:00
abstract::The als gene for alpha-acetolactate synthase of Lactococcus lactis MG1363 was cloned on a multicopy plasmid under the control of the inducible L. lactis lacA promoter. More than a hundredfold overproduction of alpha-acetolactate synthase was obtained in L. lactis under inducing conditions as compared with that of the ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.11.3967-3971.1995
更新日期:1995-11-01 00:00:00
abstract::Alpha-ketoglutarate-dependent (R)-dichlorprop dioxygenase (RdpA) and alpha-ketoglutarate-dependent (S)-dichlorprop dioxygenase (SdpA), which are involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH, were expressed and purified as His6-tagged fusion proteins from Escherichia...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02758-05
更新日期:2006-07-01 00:00:00
abstract::To get insight into the limiting factors existing for the efficient production of fungal peroxidase in filamentous fungi, the expression of the Phanerochaete chrysosporium lignin peroxidase H8 (lipA) and manganese peroxidase (MnP) H4 (mnp1) genes in Aspergillus niger has been studied. For this purpose, a protease-defi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.7.3016-3023.2000
更新日期:2000-07-01 00:00:00
abstract::The presence of Bartonella spp. was investigated in domestic ungulates grazing in communal pastures from a mountain area in northern Spain, where 18.3% (17/93) of cattle were found to be positive by PCR combined with a reverse line blot (PCR/RLB), whereas sheep (n = 133) or horses (n = 91) were found not to be infecte...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03159-14
更新日期:2015-01-01 00:00:00
abstract::Streptomyces coelicolor and Lemna minor were used as a model to study the modulation of bacterial gene expression during plant-streptomycete interactions. S. coelicolor was grown in minimal medium with and without L. minor fronds. Bacterial proteomes were analyzed by two-dimensional gel electrophoresis, and a comparis...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.4.1884-1889.2003
更新日期:2003-04-01 00:00:00
abstract::Pseudomonas fluorescens 2-79 suppresses take-all, a major root disease of wheat caused by Gaeumannomyces graminis var. tritici. The bacteria produce an antibiotic, phenazine-1-carboxylic acid (PCA), and a fluorescent pyoverdin siderophore. Previous studies have established that PCA has an important role in the biologi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.11.3270-3277.1991
更新日期:1991-11-01 00:00:00
abstract::Recent physiological work has shown that the filamentous euendolithic cyanobacterium Mastigocoleus testarum (strain BC008) is able to bore into solid carbonates using Ca²⁺-ATPases to take up Ca²⁺ from the medium at the excavation front, promoting dissolution of CaCO₃ there. It is not known, however, if this is a wides...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.06633-11
更新日期:2012-01-01 00:00:00
abstract::Rhodococcus erythropolis BG43 is able to degrade the Pseudomonas aeruginosa quorum sensing signal molecules PQS (Pseudomonas quinolone signal) [2-heptyl-3-hydroxy-4(1H)-quinolone] and HHQ [2-heptyl-4(1H)-quinolone] to anthranilic acid. Based on the hypothesis that degradation of HHQ might involve hydroxylation to PQS ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02145-15
更新日期:2015-11-01 00:00:00
abstract::Transpositional mutagenesis of Thiobacillus novellus by Tn501 was achieved by means of the incompatibility of IncP plasmids. Tn501 insertion caused three types of mutant phenotypes: isoleucine auxotrophy, lysine auxotrophy, and a reduced ability to oxidize reduced sulfur compounds and to fix CO(2). Oxidation rates for...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.6.1436-1441.1985
更新日期:1985-06-01 00:00:00
abstract::We identified a polyketide synthase (PKS) gene, pksN, from a strain of Nectria haematococca by complementing a mutant unable to synthesize a red perithecial pigment. pksN encodes a 2,106-amino-acid polypeptide with conserved motifs characteristic of type I PKS enzymatic domains: beta-ketoacyl synthase, acyltransferase...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.5.2984-2988.2004
更新日期:2004-05-01 00:00:00
abstract::During fermentative growth in natural and industrial environments, Saccharomyces cerevisiae must redistribute the available nitrogen from multiple exogenous sources to amino acids in order to suitably fulfill anabolic requirements. To exhaustively explore the management of this complex resource, we developed an advanc...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02617-16
更新日期:2017-02-15 00:00:00
abstract::The intestinal contents of 59 Zairese freshwater fish were examined for the presence of potential human enteric pathogens. Edwardsiella tarda and Plesiomonas shigelloides were isolated from 57 and 59% of them, respectively. For both microorganisms there was a significant difference between the isolation rates from lak...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.39.3.475-479.1980
更新日期:1980-03-01 00:00:00
abstract::A simplified X-ray film method for detecting bacterial production of Hgo by Escherichia coli was developed. There was excellent agreement of results between this X-ray film method and radioactive mercury volatilization assays. Although not quantitative, the X-ray film method has the advantages of being rapid, simple t...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.11.2871-2873.1988
更新日期:1988-11-01 00:00:00
abstract::Laboratory batch tests indicate that addition of sterile municipal sewage biosolids to clay soil from four depths increases the numbers of Escherichia coli isolates recoverable in EC-MUG broth (EC broth with 4-methylumbelliferyl-beta-glucuronide). This effect was most marked for the deeper soil layers, with increases ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.3.2287-2289.2006
更新日期:2006-03-01 00:00:00
abstract::We have constructed the first Escherichia coli-Veillonella shuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinical Veillonella strain. A highly transformable Veillonella strain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for future Veillonel...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00196-12
更新日期:2012-05-01 00:00:00
abstract::Purified manganese peroxidase (MnP) from the white-rot basidiomycete Phlebia radiata was found to convert in vitro milled pine wood (MPW) suspended in an aqueous reaction solution containing Tween 20, Mn(2+), Mn-chelating organic acid (malonate), and a hydrogen peroxide-generating system (glucose-glucose oxidase). The...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.67.10.4588-4593.2001
更新日期:2001-10-01 00:00:00
abstract::The objective of this study was to develop a rapid, reproducible, and robust method for detecting Salmonella enterica serotype Enteritidis in poultry samples. First, for the extraction and purification of DNA from the preenrichment culture, four methods (boiling, alkaline lysis, Nucleospin, and Dynabeads DNA Direct Sy...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.6.3456-3461.2003
更新日期:2003-06-01 00:00:00
abstract::In this study, we compared different methods-including transmission electron microscopy-and various nucleic acid labeling methods in which we used the fluorochromes 4',6'-diamidino-2-phenylindole (DAPI), 4-[3-methyl-2,3-dihydro-(benzo-1, 3-oxazole)-2-methylmethyledene]-1-(3'-trimethyl ammoniumpropyl)-quinilinium diioi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.6.2283-2289.2000
更新日期:2000-06-01 00:00:00
abstract::Products observed during anaerobic cyanide transformation are consistent with a hydrolytic pathway (HCN + H2O <--> HCONH2 + H2O <--> HCOOH + NH3). Formate, the most frequently observed product, was generally converted to bicarbonate. Formamide was rapidly hydrolyzed to formate upon exposure to the anaerobic consortium...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.9.3163-3164.1992
更新日期:1992-09-01 00:00:00
abstract::Enterococcus faecalis encodes a biotin-dependent oxaloacetate decarboxylase (OAD), which is constituted by four subunits: E. faecalis carboxyltransferase subunit OadA (termed Ef-A), membrane pump Ef-B, biotin acceptor protein Ef-D, and the novel subunit Ef-H. Our results show that in E. faecalis, subunits Ef-A, Ef-D, ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03980-12
更新日期:2013-05-01 00:00:00
abstract::Using bacteria to transform reactive corrosion products into stable compounds represents an alternative to traditional methods employed in iron conservation. Two environmental Aeromonas strains (CA23 and CU5) were used to transform ferric iron corrosion products (goethite and lepidocrocite) into stable ferrous iron-be...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02042-18
更新日期:2019-01-23 00:00:00
abstract::Recent functional genomics and genome-scale modeling approaches indicated that B(12) production in Lactobacillus reuteri could be improved by optimization of the medium. Here we show that a series of systematic single-amino-acid omissions could significantly modulate the production of B(12) from nearly undetectable le...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02487-08
更新日期:2009-06-01 00:00:00
abstract::A novel method of detecting extracellular protease activity at biofilm-substratum interfaces was developed. This method utilizes fluorescent molecules bound to cellulose substrata with a lectin. Extracellular proteases degrade the lectin and release the fluorochrome into solution. This new technique and a standard dis...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.67.9.4329-4334.2001
更新日期:2001-09-01 00:00:00