Abstract:
:Recent functional genomics and genome-scale modeling approaches indicated that B(12) production in Lactobacillus reuteri could be improved by optimization of the medium. Here we show that a series of systematic single-amino-acid omissions could significantly modulate the production of B(12) from nearly undetectable levels (with omission of isoleucine) to levels 20-fold higher than the levels previously reported (with omission of cysteine). Using cDNA microarray experiments, we analyzed the transcriptional response of L. reuteri to medium lacking cysteine. The results supported the observed high level of B(12) production and provided new avenues for future improvement of production of vitamin B(12).
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Santos F,Teusink B,Molenaar D,van Heck M,Wels M,Sieuwerts S,de Vos WM,Hugenholtz Jdoi
10.1128/AEM.02487-08subject
Has Abstractpub_date
2009-06-01 00:00:00pages
3930-6issue
12eissn
0099-2240issn
1098-5336pii
AEM.02487-08journal_volume
75pub_type
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.56.2.315-322.1990
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