Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri.

Abstract:

:Recent functional genomics and genome-scale modeling approaches indicated that B(12) production in Lactobacillus reuteri could be improved by optimization of the medium. Here we show that a series of systematic single-amino-acid omissions could significantly modulate the production of B(12) from nearly undetectable levels (with omission of isoleucine) to levels 20-fold higher than the levels previously reported (with omission of cysteine). Using cDNA microarray experiments, we analyzed the transcriptional response of L. reuteri to medium lacking cysteine. The results supported the observed high level of B(12) production and provided new avenues for future improvement of production of vitamin B(12).

journal_name

Appl Environ Microbiol

authors

Santos F,Teusink B,Molenaar D,van Heck M,Wels M,Sieuwerts S,de Vos WM,Hugenholtz J

doi

10.1128/AEM.02487-08

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

3930-6

issue

12

eissn

0099-2240

issn

1098-5336

pii

AEM.02487-08

journal_volume

75

pub_type

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