Evaluation of DNA extraction methods for use in combination with SYBR green I real-time PCR to detect Salmonella enterica serotype enteritidis in poultry.

abstract：:Production of bacteriocin activity designated pediocin PA-1 was associated with the presence of a 6.2-megadalton plasmid in Pediococcus acidilactici PAC1.0. The bacteriocin exhibited activity against P. acidilactici, P. pentosaceus, Lactobacillus plantarum, L. casei, L. bifermentans, and Leuconostoc mesenteroides subs...

journal_title：Applied and environmental microbiology

authors： Gonzalez CF,Kunka BS

更新日期：1987-10-01 00:00:00

abstract：:The Chloroflexi CL500-11 clade contributes a large proportion of the bacterial biomass in the oxygenated hypolimnia of deep lakes worldwide, including the world's largest freshwater system, the Laurentian Great Lakes. Traits that allow CL500-11 to thrive and its biogeochemical role in these environments are currently ...

journal_title：Applied and environmental microbiology

authors： Denef VJ,Mueller RS,Chiang E,Liebig JR,Vanderploeg HA

更新日期：2015-12-18 00:00:00

abstract：:Six 16S rRNA-targeted oligonucleotide probes were designed, validated, and used to quantify predominant groups of anaerobic bacteria in human fecal samples. A set of two probes was specific for species of the Bacteroides fragilis group and the species Bacteroides distasonis. Two others were designed to detect species ...

journal_title：Applied and environmental microbiology

authors： Franks AH,Harmsen HJ,Raangs GC,Jansen GJ,Schut F,Welling GW

更新日期：1998-09-01 00:00:00

abstract：:Legionella pneumophila proliferates in freshwater environments at temperatures ranging from 25 to 45°C. To investigate the preference of different sequence types (ST) for a specific temperature range, growth of L. pneumophila serogroup 1 (SG1) ST1 (environmental strains), ST47, and ST62 (disease-associated strains) wa...

journal_title：Applied and environmental microbiology

authors： van der Kooij D,Brouwer-Hanzens AJ,Veenendaal HR,Wullings BA

更新日期：2016-10-27 00:00:00

abstract：:To evaluate the microbial populations involved in the reduction of Fe(III) in an acidic, iron-rich sediment, the anaerobic flow of supplemental carbon and reductant was evaluated in sediment microcosms at the in situ temperature of 12 degrees C. Supplemental glucose and cellobiose stimulated the formation of Fe(II); 4...

journal_title：Applied and environmental microbiology

authors： Küsel K,Dorsch T,Acker G,Stackebrandt E

更新日期：1999-08-01 00:00:00

abstract：:The multistep cleavage of carotenoids in Mucorales during the sexual phase results in a cocktail of trisporic acid (C18) sex pheromones. We hypothesized that the C18 trisporoid intermediates have a specific regulatory function for sex pheromone production and carotenogenesis that varies with genus/species and vegetati...

journal_title：Applied and environmental microbiology

authors： Sahadevan Y,Richter-Fecken M,Kaerger K,Voigt K,Boland W

更新日期：2013-12-01 00:00:00

abstract：:Aryl-alcohol oxidase (AAO) is an extracellular flavoprotein that supplies ligninolytic peroxidases with H2O2 during natural wood decay. With a broad substrate specificity and highly stereoselective reaction mechanism, AAO is an attractive candidate for studies into organic synthesis and synthetic biology, and yet the ...

journal_title：Applied and environmental microbiology

authors： Viña-Gonzalez J,Gonzalez-Perez D,Ferreira P,Martinez AT,Alcalde M

更新日期：2015-09-01 00:00:00

abstract：:We determined the effects of grain size and nutritional conditions on the penetration rate and metabolic activity of Escherichia coli strains in anaerobic, nutrient-saturated chambers packed with different sizes of glass beads (diameters, 116 to 767 mum) under static conditions. The chambers had nearly equal porositie...

journal_title：Applied and environmental microbiology

authors： Sharma PK,McInerney MJ

更新日期：1994-05-01 00:00:00

abstract：:A mutant of Pseudomonas sp. strain HBP1, originally isolated on 2-hydroxybiphenyl, was selected for the ability to grow on 2-propylphenol as the sole carbon and energy source. In the mutant strain, which was designated as Pseudomonas sp. strain HBP1 Prp, the cellular induction mechanism involved in the synthesis of th...

journal_title：Applied and environmental microbiology

authors： Kohler HP,van der Maarel MJ,Kohler-Staub D

更新日期：1993-03-01 00:00:00

abstract：:Adsorption of Agrobacterium tumefaciens cells to potato tuber disks reached equilibrium after coincubation for about 30 min. More than 10% of the number of bacteria bound at equilibrium were adsorbed within 30 s. Adsorption isotherms obtained at three temperatures showed that the heat of adsorption was nearly zero. ...

journal_title：Applied and environmental microbiology

authors： Kluepfel DA,Pueppke SG

更新日期：1986-05-01 00:00:00

abstract：:Noroviruses from mussels collected near sewage effluents were compared with local patient outbreak strains. Sequence analyses of RNA polymerase-capsid-poly(A)-3' (3.1-kilobase) regions confirmed the 99.9% similarity between genotype I.1 strains from mussels and patient strains from recreational-bathing outbreaks, indi...

journal_title：Applied and environmental microbiology

authors： Nenonen NP,Hannoun C,Horal P,Hernroth B,Bergström T

更新日期：2008-04-01 00:00:00

abstract：:Streptococcus suis is an important pathogen of pigs and may cause serious disease in humans. Serotyping is an important tool for detection and epidemiological studies of S. suis Thirty-three reference serotypes and nine novel cps loci (NCLs) are recognized in S. suis To gain a better understanding of the prevalence an...

journal_title：Applied and environmental microbiology

authors： Qiu X,Bai X,Lan R,Zheng H,Xu J

更新日期：2016-11-21 00:00:00

abstract：:In samples of flooded soil containing blue-green algae (cyanobacteria), the presence of rice plants did not influence the nitrogenase activity of the algae. Nitrogenase activity of heterotrophic bacteria was enhanced by the presence of rice plants, but this activity was not affected by changes in plant density. The ra...

journal_title：Applied and environmental microbiology

authors： Habte M,Alexander M

更新日期：1980-09-01 00:00:00

abstract：:Two different DNA fragments, one of 2.9 kilobases and the other of 5.1 kilobases, were cloned from Phytophthora citrophthora and showed no homology with DNA from plants and other related fungi. These DNA probes hybridized with DNA from 12 different P. citrophthora isolates obtained from a variety of hosts but did not ...

journal_title：Applied and environmental microbiology

authors： Goodwin PH,Kirkpatrick BC,Duniway JM

更新日期：1990-03-01 00:00:00

abstract：:Receptors on the cell surfaces of bacterial hosts are essential during the infection cycle of bacteriophages. To date, the phage receptors of the industrial relevant dairy starter bacterium Streptococcus thermophilus remain elusive. Thus, we set out to identify cell surface structures that are involved in host recogni...

journal_title：Applied and environmental microbiology

authors： Szymczak P,Filipe SR,Covas G,Vogensen FK,Neves AR,Janzen T

更新日期：2018-11-15 00:00:00

abstract：:Previously, we demonstrated conjugal transfer of a specially constructed shuttle vector, pRDB5, from the human colonic anaerobe Bacteroides uniformis to the ruminal anaerobe Prevotella (Bacteroides) ruminicola B(1)4. We have now shown that naturally occurring gene transfer elements in Bacteroides species and Prevotell...

journal_title：Applied and environmental microbiology

authors： Shoemaker NB,Wang GR,Salyers AA

更新日期：1992-04-01 00:00:00

abstract：:Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not fully understood. To address this lack of information, a study of the epidemiology and ecology of V. cholerae O1 and O139...

journal_title：Applied and environmental microbiology

authors： Alam M,Hasan NA,Sadique A,Bhuiyan NA,Ahmed KU,Nusrin S,Nair GB,Siddique AK,Sack RB,Sack DA,Huq A,Colwell RR

更新日期：2006-06-01 00:00:00

abstract：:A radiometric method for the detection of Salmonella in foods has been developed which is based on Salmonella poly H agglutinating serum preventing Salmonella from producing 14CO2 from [14C]dulcitol. The method will detect the presence or absence of Salmonella in a product within 30 h compared to 4 to 5 days by routin...

journal_title：Applied and environmental microbiology

authors： Stewart BJ,Eyles MJ,Murrell WG

更新日期：1980-08-01 00:00:00

abstract：:Low-temperature ecosystems represent the largest biosphere on Earth, and yet our understanding of the roles of bacteriophages in these systems is limited. Here, the influence of the cold-active filamentous phage SW1 on the phenotype and gene transcription of its host, Shewanella piezotolerans WP3 (WP3), was investigat...

journal_title：Applied and environmental microbiology

authors： Jian H,Xiao X,Wang F

更新日期：2013-11-01 00:00:00

abstract：:We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background bu...

journal_title：Applied and environmental microbiology

authors： Charles TC,Doty SL,Nester EW

更新日期：1994-11-01 00:00:00

abstract：:Cholesterol and albumin are limiting factors in the growth of Mycoplasma species. These nutrients are usually supplied in the culture medium by the addition of serum. The growth of M. pneumoniae in a serum-free medium containing an ethanolic cholesterol suspension and albumin was about one-half the level attained in s...

journal_title：Applied and environmental microbiology

authors： Cluss RG,Johnson JK,Somerson NL

更新日期：1983-08-01 00:00:00

abstract：:We report here the cloning and sequencing of the gene for proline dehydrogenase (putA) of Bradyrhizobium japonicum. An open reading frame coding for 1,016 amino acids was identified. The B. japonicum gene codes for a bifunctional protein with proline dehydrogenase and pyrroline-5-carboxylate (P5C) dehydrogenase activi...

journal_title：Applied and environmental microbiology

authors： Straub PF,Reynolds PH,Althomsons S,Mett V,Zhu Y,Shearer G,Kohl DH

更新日期：1996-01-01 00:00:00

abstract：:This work demonstrates the ability of a bacterial concentration and recovery procedure combined with three different PCR assays targeting the lacZ, wecG, and 16S rRNA genes, respectively, to detect the presence of total coliforms in 100-ml samples of potable water (presence/absence test). PCR assays were first compare...

journal_title：Applied and environmental microbiology

authors： Maheux AF,Boudreau DK,Bisson MA,Dion-Dupont V,Bouchard S,Nkuranga M,Bergeron MG,Rodriguez MJ

更新日期：2014-07-01 00:00:00

abstract：:Ophthalmic acid (OA; l-γ-glutamyl-l-2-aminobutyryl-glycine) is an analog of glutathione (GSH; l-γ-glutamyl-l-cysteinyl-glycine) in which the cysteine moiety is replaced by l-2-aminobutyrate. OA is a useful peptide for the pharmaceutical and/or food industries. Herein, we report a method for the production of OA using ...

journal_title：Applied and environmental microbiology

authors： Ito T,Tokoro M,Hori R,Hemmi H,Yoshimura T

更新日期：2018-03-19 00:00:00

abstract：:A census of clean room surface-associated bacterial populations was derived from the results of both the cloning and sequencing of 16S rRNA genes and DNA microarray (PhyloChip) analyses. Samples from the Lockheed Martin Aeronautics Multiple Testing Facility (LMA-MTF), the Kennedy Space Center Payload Hazard and Servic...

journal_title：Applied and environmental microbiology

authors： La Duc MT,Osman S,Vaishampayan P,Piceno Y,Andersen G,Spry JA,Venkateswaran K

更新日期：2009-10-01 00:00:00

abstract：:We have previously reported that an acid tolerance response (ATR) can be induced in Streptococcus macedonicus cells at mid-log phase after autoacidification, transient exposure to acidic pH, or acid habituation, as well as at stationary phase. Here, we compared the transcriptional profiles of these epigenetic phenotyp...

journal_title：Applied and environmental microbiology

authors： Papadimitriou K,Boutou E,Zoumpopoulou G,Tarantilis PA,Polissiou M,Vorgias CE,Tsakalidou E

更新日期：2008-10-01 00:00:00

abstract：:The pigmented strain Serratia marcescens ATCC 274 had a higher buffering capacity and a higher membrane H conductance than S. marcescens GP, a spontaneous nonpigmented mutant of ATCC 274. The data suggest that mutations which apparently affect only the synthesis of a secondary metabolite can modify buffering capacity ...

journal_title：Applied and environmental microbiology

authors： Rius N,Solé M,Francia A,Lorén JG

更新日期：1994-06-01 00:00:00

abstract：:The low-copy-number 8.7-kb plasmid pGIF3 of Streptomyces incarnatus was studied after cloning in the Escherichia coli vector pBR322; a restriction map was constructed. Southern blot analysis showed that pGIF3 in S. incarnatus occurs predominantly as integrated in a larger replicon. The plasmid carries a gene for thios...

journal_title：Applied and environmental microbiology

authors： Malina H,Robert-Gero M

更新日期：1992-03-01 00:00:00

abstract：:PCR-based methods have been developed to rapidly screen for Legionella pneumophila in water as an alternative to time-consuming culture techniques. However, these methods fail to discriminate between live and dead bacteria. Here, we report a viability assay (viability PCR [v-PCR]) for L. pneumophila that combines ethi...

journal_title：Applied and environmental microbiology

authors： Delgado-Viscogliosi P,Solignac L,Delattre JM

更新日期：2009-06-01 00:00:00

abstract：:Two genetically engineered microorganisms (GEMs), Pseudomonas sp. strain B13 FR1(pFRC20P) (FR120) and Pseudomonas putida KT2440(pWWO-EB62) (EB62), were introduced into activated sludge microcosms that had the level of aeration, nutrient makeup, and microbial community structure of activated sludge reactors. FR120 cont...

journal_title：Applied and environmental microbiology

authors： Nüsslein K,Maris D,Timmis K,Dwyer DF

更新日期：1992-10-01 00:00:00