Comprehensive census of bacteria in clean rooms by using DNA microarray and cloning methods.

abstract：:Viral pollution in shellfish has been analyzed simultaneously across a wide range of geographical regions, with emphasis on the concomitant variations in physicochemical characteristics and social features. The methods for sample treatment and for the detection of human enteric viruses were optimized by the participat...

journal_title：Applied and environmental microbiology

authors： Formiga-Cruz M,Tofiño-Quesada G,Bofill-Mas S,Lees DN,Henshilwood K,Allard AK,Conden-Hansson AC,Hernroth BE,Vantarakis A,Tsibouxi A,Papapetropoulou M,Furones MD,Girones R

更新日期：2002-12-01 00:00:00

abstract：:Studies have shown that certain opportunistic pathogenic species of nontuberculous mycobacteria (NTM) can be present in distributed drinking water. However, detailed information about NTM population composition in drinking water is lacking. Therefore, NTM communities in unchlorinated drinking water from the distributi...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Heijnen L,van der Kooij D

更新日期：2013-10-01 00:00:00

abstract：:Lactococcus lactis is a prokaryotic microorganism with great importance as a culture starter and has become the model species among the lactic acid bacteria. The long and safe history of use of L. lactis in dairy fermentations has resulted in the classification of this species as GRAS (General Regarded As Safe) or QPS...

journal_title：Applied and environmental microbiology

authors： Ladero V,Rattray FP,Mayo B,Martín MC,Fernández M,Alvarez MA

更新日期：2011-09-01 00:00:00

abstract：:A newly isolated rod-shaped, gram-negative anaerobic bacterium from human feces, named Julong 732, was found to be capable of metabolizing the isoflavone dihydrodaidzein to S-equol under anaerobic conditions. The metabolite, equol, was identified by using electron impact ionization mass spectrometry, (1)H and (13)C nu...

journal_title：Applied and environmental microbiology

authors： Wang XL,Hur HG,Lee JH,Kim KT,Kim SI

更新日期：2005-01-01 00:00:00

abstract：:Marine microbiologists commonly assay lipase activities by using a synthetic fluorescent analog, 4-methylumbelliferyl (MUF)-oleate. The technique is convenient, but it is considered to be unspecific because of the structure of this analog. This study reports the design of a new specific and sensitive lipase assay base...

journal_title：Applied and environmental microbiology

authors： Bourguet N,Torréton JP,Galy O,Arondel V,Goutx M

更新日期：2003-12-01 00:00:00

abstract：:We report the identification of novel archaea living in close association with bacteria in the cold (approximately 10 degrees C) sulfurous marsh water of the Sippenauer Moor near Regensburg, Bavaria, Germany. These microorganisms form a characteristic, macroscopically visible structure, morphologically comparable to a...

journal_title：Applied and environmental microbiology

authors： Rudolph C,Wanner G,Huber R

更新日期：2001-05-01 00:00:00

abstract：:The diversity of deep-sea high-pressure-adapted (piezophilic) microbes in isolated monoculture remains low. In this study, a novel obligately psychropiezophilic bacterium was isolated from seawater collected from the Puerto Rico Trench at a depth of ∼6,000 m. This isolate, designated YC-1, grew best in a nutrient-rich...

journal_title：Applied and environmental microbiology

authors： Cao Y,Chastain RA,Eloe EA,Nogi Y,Kato C,Bartlett DH

更新日期：2014-01-01 00:00:00

abstract：:Leptospirosis, an emerging zoonotic disease, remains poorly understood because of a lack of genetic manipulation tools available for pathogenic leptospires. Current genetic manipulation techniques include insertion of DNA by random transposon mutagenesis and homologous recombination via suicide vectors. This study des...

journal_title：Applied and environmental microbiology

authors： Pappas CJ,Benaroudj N,Picardeau M

更新日期：2015-05-01 00:00:00

abstract：:Growth, survival, and pathogenicity of Klebsiella growing in and on environmental foci were examined. Total coliforms present in raw wastes from pulp mills were in excess of 10(5)/ml, and 60 to 80% were Klebsiella. Fecal coliform counts ranged from 10(1) to 10(5)/ml. Klebsiella isolates from industrial effluents and a...

journal_title：Applied and environmental microbiology

authors： Knittel MD,Seidler RJ,Eby C,Cabe LM

更新日期：1977-11-01 00:00:00

abstract：:The vaginal mucosa of ovariectomized female rats has been examined by scanning electron microscopy before and after estrogen [1,3,5(10)-estratriene-3,17beta-diol] treatment. Without estrogen stimulation vaginal colonization is minimal and the epithelium is characterized by a layer of epithelial cells covered with smal...

journal_title：Applied and environmental microbiology

authors： Larsen B,Markovetz AJ,Galask RP

更新日期：1977-07-01 00:00:00

abstract：:Genome sequencing of the thermophilic archaeon Pyrococcus horikoshii OT3 revealed a gene which had high sequence similarity to the gene encoding the carboxypeptidase of Sulfolobus solfataricus and also to that encoding the aminoacylase from Bacillus stearothermophilus. The gene from P. horikoshii comprises an open rea...

journal_title：Applied and environmental microbiology

authors： Ishikawa K,Ishida H,Matsui I,Kawarabayasi Y,Kikuchi H

更新日期：2001-02-01 00:00:00

abstract：:Maximum yields of amylase were produced by the thermophilic actinomycete Thermomonospora viridis in a modified Simpson and McCoy medium containing 1.5% corn starch and 0.5% mycological peptone with an initial pH 7.0. Best yields of amylase were obtained after incubation for 48 h, when the pH of the medium had risen to...

journal_title：Applied and environmental microbiology

authors： Upton ME,Fogarty WM

更新日期：1977-01-01 00:00:00

abstract：:Nutritional and physical factors which influence the germination of akinetes of Nodularia spumigena (Cyanobacteriaceae) were examined. Low concentrations of phosphorus (<0.9 muM) were required for germination. Nitrate had no effect, but ammonia, at concentrations of >45 muM, inhibited germination. Salinities of >20 pe...

journal_title：Applied and environmental microbiology

authors： Huber AL

更新日期：1985-01-01 00:00:00

abstract：:Nervous necrosis virus (NNV) is a member of the Betanodavirus genus that causes fatal diseases in over 40 species of fish worldwide. Mortality among NNV-infected fish larvae is almost 100%. In order to elucidate the mechanisms responsible for the susceptibility of fish larvae to NNV, we exposed zebrafish larvae to NNV...

journal_title：Applied and environmental microbiology

authors： Morick D,Faigenbaum O,Smirnov M,Fellig Y,Inbal A,Kotler M

更新日期：2015-05-15 00:00:00

abstract：:Primers obtained from gene sequences coding for heat shock proteins (HSP) were used to specifically detect enteric protozoans of the genus Giardia. The HSP primers amplified Giardia DNA or the corresponding RNA sequences obtained from lysed cysts and gave a 163-bp product. Since the presence of the product did not ind...

journal_title：Applied and environmental microbiology

authors： Abbaszadegan M,Huber MS,Gerba CP,Pepper IL

更新日期：1997-01-01 00:00:00

abstract：:The membrane proteins IIC and IID of the mannose phosphotransferase system (Man-PTS) together form a membrane-located complex that serves as a receptor for several different bacteriocins, including the pediocin-like class IIa bacteriocins and the class IIc bacteriocin lactococcin A. Bacterial strains sensitive to clas...

journal_title：Applied and environmental microbiology

authors： Kjos M,Nes IF,Diep DB

更新日期：2011-05-01 00:00:00

abstract：:The microaerophilic bacterium Campylobacter jejuni is the most common cause of bacterial food-borne infections in the developed world. Tolerance to environmental stress relies on proteases and chaperones in the cell envelope, such as HtrA and SurA. HtrA displays both chaperone and protease activities, but little is kn...

journal_title：Applied and environmental microbiology

authors： Baek KT,Vegge CS,Skórko-Glonek J,Brøndsted L

更新日期：2011-01-01 00:00:00

abstract：:Superoxide dismutase (SOD) activity was determined during the growth cycle of unheated and heat-injured cells of Staphylococcus aureus MF-31. SOd activity levels dropped in unheated cells during the lag phase, increased during logarithmic phase, and became constant in the stationary phase. Cells which were sublethally...

journal_title：Applied and environmental microbiology

authors： Bucker ER,Martin SE

更新日期：1981-03-01 00:00:00

abstract：:Denitrifying microbial communities and denitrification in salt marsh sediments may be affected by many factors, including environmental conditions, nutrient availability, and levels of pollutants. The objective of this study was to examine how microbial community composition and denitrification enzyme activities (DEA)...

journal_title：Applied and environmental microbiology

authors： Cao Y,Green PG,Holden PA

更新日期：2008-12-01 00:00:00

abstract：:Cyclic lipopeptides (CLPs) are produced by many Pseudomonas species and have several biological functions, including a role in surface motility, biofilm formation, virulence, and antimicrobial activity. This study focused on the diversity and role of LuxR-type transcriptional regulators in CLP biosynthesis in Pseudomo...

journal_title：Applied and environmental microbiology

authors： de Bruijn I,Raaijmakers JM

更新日期：2009-07-01 00:00:00

abstract：:Spores of vesicular arbuscular mycorrhizal (VAM) fungi contain thousands of nuclei. In order to understand the karyotic structure of a VAM fungus spore, the genetic variation of the first generation of spores from a VAM fungus (Gigaspora margarita) was examined. Spores originating from both single- and multispore inoc...

journal_title：Applied and environmental microbiology

authors： Zeze A,Sulistyowati E,Ophel-Keller K,Barker S,Smith S

更新日期：1997-02-01 00:00:00

abstract：:Gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy were used to study the metabolism of deuterated n-alkanes (C6 to C12) and 1-13C-labeled n-hexane by a highly enriched sulfate-reducing bacterial culture. All substrates were activated via fumarate addition to form the corresponding alkyls...

journal_title：Applied and environmental microbiology

authors： Davidova IA,Gieg LM,Nanny M,Kropp KG,Suflita JM

更新日期：2005-12-01 00:00:00

abstract：:Tropomyosin receptor kinase A (TrkA), a receptor tyrosine kinase, is known to be associated with various diseases. Thus, TrkA has become a major drug-screening target for these diseases. Despite the fact that the production of recombinant proteins by prokaryotic hosts has advantages, such as fast growth and ease of ge...

journal_title：Applied and environmental microbiology

authors： Honda T,Yasuda T,Tanaka T,Hagiwara K,Arai T,Yoshino T

更新日期：2015-02-01 00:00:00

abstract：:Intestinal pathogenic Escherichia coli represents a global health problem for mammals, including humans. At present, diarrheagenic E. coli bacteria are grouped into seven major pathotypes that differ in their virulence factor profiles, severity of clinical manifestations, and prognosis. In this study, we developed and...

journal_title：Applied and environmental microbiology

authors： Müller D,Greune L,Heusipp G,Karch H,Fruth A,Tschäpe H,Schmidt MA

更新日期：2007-05-01 00:00:00

abstract：:The stability of spheroplasts from the osmotrophic yeast Saccharomyces rouxii was studied in buffered solutions of mannitol and glucose. The plasma membranes from cells grown in high glucose concentrations were more stable to osmotic lysis than were membranes from cells grown in lower glucose concentrations. Mannitol ...

journal_title：Applied and environmental microbiology

authors： Moran JW,Witter LD

更新日期：1980-04-01 00:00:00

abstract：:We describe here a new enzyme-coupled assay for the quantitation of d-xylose using readily available enzymes that allows kinetic evaluation of hemicellulolytic enzymes using natural xylooligosaccharide substrates. Hydrogen peroxide is generated as an intermediary analyte, which allows flexibility in the choice of the ...

journal_title：Applied and environmental microbiology

authors： Wagschal K,Franqui-Espiet D,Lee CC,Robertson GH,Wong DW

更新日期：2005-09-01 00:00:00

abstract：:Monitoring cell growth and measuring physical features of food-borne pathogenic bacteria are important for better understanding the conditions under which these organisms survive and proliferate. To address this challenge, buoyant masses of live and dead Escherichia coli O157:H7 and Listeria innocua were measured usin...

journal_title：Applied and environmental microbiology

authors： Lewis CL,Craig CC,Senecal AG

更新日期：2014-06-01 00:00:00

abstract：:The scarcity of fresh water in the Mediterranean region necessitates the search for halotolerant agents of biological control of plant diseases that can be applied in arid-zone agriculture irrigated with saline water. Among 29 Trichoderma strains previously isolated from Mediterranean Psammocinia sp. sponges, the grea...

journal_title：Applied and environmental microbiology

authors： Gal-Hemed I,Atanasova L,Komon-Zelazowska M,Druzhinina IS,Viterbo A,Yarden O

更新日期：2011-08-01 00:00:00

abstract：:We examined the competitive relationship between two isolates of denitrifying bacteria, both of which grow well under aerobic conditions but differ in their ability to grow under denitrifying conditions. The growth and persistence of the two isolates, added to sterile soil or added to soil previously colonized by the ...

journal_title：Applied and environmental microbiology

authors： Murray RE,Parsons LL,Smith MS

更新日期：1992-12-01 00:00:00

abstract：:The objective of this study was to explore the long-term effects of different organic and inorganic fertilizers on activity and composition of the denitrifying and total bacterial communities in arable soil. Soil from the following six treatments was analyzed in an experimental field site established in 1956: cattle m...

journal_title：Applied and environmental microbiology

authors： Enwall K,Philippot L,Hallin S

更新日期：2005-12-01 00:00:00