Natural communities of novel archaea and bacteria growing in cold sulfurous springs with a string-of-pearls-like morphology.

Abstract:

:We report the identification of novel archaea living in close association with bacteria in the cold (approximately 10 degrees C) sulfurous marsh water of the Sippenauer Moor near Regensburg, Bavaria, Germany. These microorganisms form a characteristic, macroscopically visible structure, morphologically comparable to a string of pearls. Tiny, whitish globules (the pearls; diameter, about 0.5 to 3.0 mm) are connected to each other by thin, white-colored threads. Fluorescent in situ hybridization (FISH) studies have revealed that the outer part of the pearls is mainly composed of bacteria, with a filamentous bacterium predominating. Internally, archaeal cocci are the predominant microorganisms, with up to 10(7) cells estimated to be present in a single pearl. The archaea appear to be embedded in a polymer of unknown chemical composition. According to FISH and 16S rRNA gene sequence analysis, the archaea are affiliated with the euryarchaeal kingdom. The new euryarchaeal sequence represents a deep phylogenetic branch within the 16S rRNA tree and does not show extensive similarity to any cultivated archaea or to 16S rRNA gene sequences from environmental samples.

journal_name

Appl Environ Microbiol

authors

Rudolph C,Wanner G,Huber R

doi

10.1128/AEM.67.5.2336-2344.2001

keywords:

subject

Has Abstract

pub_date

2001-05-01 00:00:00

pages

2336-44

issue

5

eissn

0099-2240

issn

1098-5336

journal_volume

67

pub_type

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