Exposure of Saccharomyces cerevisiae to acetaldehyde induces sulfur amino acid metabolism and polyamine transporter genes, which depend on Met4p and Haa1p transcription factors, respectively.

abstract：:A toluene-degrading sulfate-reducing bacterium, strain Tol2, was isolated from marine sediment under strictly anoxic conditions. Toluene was toxic if applied directly to the medium at concentrations higher than 0.5 mM. To provide toluene continuously at a nontoxic concentration, it was supplied in an inert hydrophobic...

journal_title：Applied and environmental microbiology

authors： Rabus R,Nordhaus R,Ludwig W,Widdel F

更新日期：1993-05-01 00:00:00

abstract：:Flux balance analysis and phenotypic data were used to provide clues to the relationships between the activities of gene products and the phenotypes resulting from the deletion of genes involved in respiratory function in Saccharomyces cerevisiae. The effect of partial or complete respiratory deficiency on the ethanol...

journal_title：Applied and environmental microbiology

authors： Dikicioglu D,Pir P,Onsan ZI,Ulgen KO,Kirdar B,Oliver SG

更新日期：2008-09-01 00:00:00

abstract：:To obtain molecular insights into the action mode of antimicrobial activity of pediocin PA-1, the interactions between this bacteriocin and dimyristoylphosphatidylcholine (DMPC) or dimyristoylphosphatidylglycerol (DMPG) model membranes have been investigated in D(2)O at pD 6 by Fourier transform infrared spectroscopy....

journal_title：Applied and environmental microbiology

authors： Gaussier H,Lefèvre T,Subirade M

更新日期：2003-11-01 00:00:00

abstract：:Cry1Fa insecticidal protein was successfully radiolabeled with (125)I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran species Ostrinia nubilalis, Spodoptera frugiperda, Spodoptera exigua, Helicoverpa armigera, Heliothis virescens, and Plutella xylostella. Homologous competition as...

journal_title：Applied and environmental microbiology

authors： Hernández-Rodríguez CS,Hernández-Martínez P,Van Rie J,Escriche B,Ferré J

更新日期：2012-06-01 00:00:00

abstract：:Although there have been several reports on the efficacy assessment of a Salmonella enterica serovar Enteritidis vaccine against intestinal and parenchymatous organ diseases of laying hens, no public health risk characterization of its long-term effect on eggs has been reported. In this study, we attempted to assess t...

journal_title：Applied and environmental microbiology

authors： Toyota-Hanatani Y,Ekawa T,Ohta H,Igimi S,Hara-Kudo Y,Sasai K,Baba E

更新日期：2009-02-01 00:00:00

abstract：:Resistance to Bacillus thuringiensis Cry1Ac toxin was characterized in a population of Helicoverpa zea larvae previously shown not to have an alteration in toxin binding as the primary resistance mechanism to this toxin. Cry1Ac-selected larvae (AR1) were resistant to protoxins and toxins of Cry1Ab, Cry1Ac, and the cor...

journal_title：Applied and environmental microbiology

authors： Caccia S,Moar WJ,Chandrashekhar J,Oppert C,Anilkumar KJ,Jurat-Fuentes JL,Ferré J

更新日期：2012-08-01 00:00:00

abstract：:Five genes essential for folate biosynthesis in Lactococcus lactis were cloned on a broad-host-range lactococcal vector and were transferred to the folate auxotroph Lactobacillus gasseri. As a result L. gasseri changed from a folate consumer to a folate producer. This principle can be used to increase folate levels in...

journal_title：Applied and environmental microbiology

authors： Wegkamp A,Starrenburg M,de Vos WM,Hugenholtz J,Sybesma W

更新日期：2004-05-01 00:00:00

abstract：:The Association of Official Analytical Chemists (AOAC) test for assessing the tuberculocidal activity of disinfectants has been shown to be variable. A modified AOAC test, which substituted Middlebrook 7H9 broth as the primary subculture medium and used neutralization by dilution, was compared with the standard AOAC m...

journal_title：Applied and environmental microbiology

authors： Cole EC,Rutala WA,Nessen L,Wannamaker NS,Weber DJ

更新日期：1990-06-01 00:00:00

abstract：:Clostridium thermohydrosulfuricum 39E produced a cell-bound cyclodextrin (CD)-degrading enzyme (cyclodextrinase). It was partially purified 205-fold (specific activity, 14.5 U/mg of protein) by solubilizing with Triton X-100, ammonium sulfate treatment, and DEAE-Sepharose CL-6B column chromatography. The enzyme activi...

journal_title：Applied and environmental microbiology

authors： Saha BC,Zeikus JG

更新日期：1990-09-01 00:00:00

abstract：:Rhizobiophage V, isolated from soil in the vicinity of soybean roots, was strongly lytic on Bradyrhizobium japonicum 123B (USDA 123) but only mildly lytic on strain L4-4, a chemically induced small-colony mutant of 123. Numerous bacteriophage-resistant variants were isolated from L4-4 infected with phage V; two were s...

journal_title：Applied and environmental microbiology

authors： Abebe HM,Sadowsky MJ,Kinkle BK,Schmidt EL

更新日期：1992-10-01 00:00:00

abstract：:LeLoir glycosyltransferases are important biocatalysts for the production of glycosidic bonds in natural products, chiral building blocks, and pharmaceuticals. Trehalose transferase (TreT) is of particular interest since it catalyzes the stereo- and enantioselective α,α-(1→1) coupling of a nucleotide sugar donor and m...

journal_title：Applied and environmental microbiology

authors： Mestrom L,Marsden SR,Dieters M,Achterberg P,Stolk L,Bento I,Hanefeld U,Hagedoorn PL

更新日期：2019-04-04 00:00:00

abstract：:The seasonal variations in community structure and cell morphology of pelagic procaryotes from a high mountain lake (Gossenkollesee, Austria) were studied by in situ hybridization with rRNA-targeted fluorescently labeled oligonucleotide probes (FISH) and image-analyzed microscopy. Compositional changes and biomass flu...

journal_title：Applied and environmental microbiology

authors： Pernthaler J,Glockner FO,Unterholzner S,Alfreider A,Psenner R,Amann R

更新日期：1998-11-01 00:00:00

abstract：:The spatial arrangements and associative behavior of Actinomyces naeslundii, Veillonella dispar, Fusobacterium nucleatum, Streptococcus sobrinus, and Streptococcus oralis strains in an in vitro model of supragingival plaque were determined. Using species-specific fluorescence-labeled antibodies in conjunction with con...

journal_title：Applied and environmental microbiology

authors： Guggenheim M,Shapiro S,Gmür R,Guggenheim B

更新日期：2001-03-01 00:00:00

abstract：:DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellob...

journal_title：Applied and environmental microbiology

authors： Coleman SS,Melanson DM,Biosca EG,Oliver JD

更新日期：1996-04-01 00:00:00

abstract：:The fungus Cladosporium sphaerospermum was isolated from a biofilter used for the removal of toluene from waste gases. This is the first report describing growth of a eukaryotic organism with toluene as the sole source of carbon and energy. The oxygen consumption rates, as well as the measured enzyme activities, of to...

journal_title：Applied and environmental microbiology

authors： Weber FJ,Hage KC,de Bont JA

更新日期：1995-10-01 00:00:00

abstract：:A new method for the extraction of bacterial DNA from soil has been developed. Soil samples of 50 g were dispersed, and bacteria were released by use of a cation-exchange resin; subsequently, bacteria were separated from soil particles by low-speed centrifugation and lysed with lysozyme and ionic detergent, and the DN...

journal_title：Applied and environmental microbiology

authors： Jacobsen CS,Rasmussen OF

更新日期：1992-08-01 00:00:00

abstract：:Aerobic and anaerobic microorganisms were enumerated in tubercles collected from sections of the water distribution pipeline in the Columbus, Ohio, metropolitan area. Coliform bacteria were not detected in the tubercles examined. Sulfate-reducing bacteria were detected in 80% of the samples. Nitrate-reducing heterotro...

journal_title：Applied and environmental microbiology

authors： Tuovinen OH,Hsu JC

更新日期：1982-09-01 00:00:00

abstract：:Biodegradation experiments with radioactively labeled trichloroethylene showed that 32% of the radioactive carbon was converted to glyoxylic acid, dichloroacetic acid and trichloroacetic acid and that the same percentage was converted to CO2 and CO after 140 h of incubation by a pure culture of a type II methane-utili...

journal_title：Applied and environmental microbiology

authors： Uchiyama H,Nakajima T,Yagi O,Nakahara T

更新日期：1992-09-01 00:00:00

abstract：:Algal biofuels represent one of the most promising means of sustainably replacing liquid fuels. However, significant challenges remain before alga-based fuels become competitive with fossil fuels. One of the largest challenges is the ability to harvest the algae in an economical and low-energy manner. In this article,...

journal_title：Applied and environmental microbiology

authors： Powell RJ,Hill RT

更新日期：2013-10-01 00:00:00

abstract：:Dibenzothiophene (DBT) and its derivatives can be microbially desulfurized by enzymes DszC, DszA, and DszB, which are encoded by the operon dszABC and contribute to the conversion in tandem. We investigated the expression characteristics of the dsz operon. Our results revealed that the levels of transcription and tran...

journal_title：Applied and environmental microbiology

authors： Li GQ,Li SS,Zhang ML,Wang J,Zhu L,Liang FL,Liu RL,Ma T

更新日期：2008-02-01 00:00:00

abstract：:Toxin-producing isolates of Bacillus licheniformis were obtained from foods involved in food poisoning incidents, from raw milk, and from industrially produced baby food. The toxin detection method, based on the inhibition of boar spermatozoan motility, has been shown previously to be a sensitive assay for the emetic ...

journal_title：Applied and environmental microbiology

authors： Salkinoja-Salonen MS,Vuorio R,Andersson MA,Kämpfer P,Andersson MC,Honkanen-Buzalski T,Scoging AC

更新日期：1999-10-01 00:00:00

abstract：:Evaluation of the fate and transport of biological warfare (BW) agents in landfills requires the development of specific and sensitive detection assays. The objective of the current study was to develop and validate SYBR green quantitative real-time PCR (Q-PCR) assays for the specific detection and quantification of s...

journal_title：Applied and environmental microbiology

authors： Saikaly PE,Barlaz MA,de Los Reyes FL 3rd

更新日期：2007-10-01 00:00:00

abstract：:Shewanella oneidensis MR-1 is a facultative anaerobe that derives energy by coupling organic matter oxidation to the reduction of a wide range of electron acceptors. Here, we quantitatively assessed the lactate and pyruvate metabolism of MR-1 under three distinct conditions: electron acceptor-limited growth on lactate...

journal_title：Applied and environmental microbiology

authors： Pinchuk GE,Geydebrekht OV,Hill EA,Reed JL,Konopka AE,Beliaev AS,Fredrickson JK

更新日期：2011-12-01 00:00:00

abstract：:Obligate aerobic organisms rely on a functional electron transport chain for energy conservation and NADH oxidation. Because of this essential requirement, the genes of this pathway are likely constitutively and highly expressed to avoid a cofactor imbalance and energy shortage under fluctuating environmental conditio...

journal_title：Applied and environmental microbiology

authors： Nies SC,Dinger R,Chen Y,Wordofa GG,Kristensen M,Schneider K,Büchs J,Petzold CJ,Keasling JD,Blank LM,Ebert BE

更新日期：2020-05-19 00:00:00

abstract：:Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on eithe...

journal_title：Applied and environmental microbiology

authors： Lauber CL,Hamady M,Knight R,Fierer N

更新日期：2009-08-01 00:00:00

abstract：:Bacteria able to utilize Aspon, Azodrin, Dasanit, diazinon, malathion, Orthene, parathion, Trithion, dimethoate, Dylox, methyl parathion, and Vapona as sole phosphorus sources were isolated from soil and sewage. Individual isolates used from 3 to 10 of these insecticides as sole phosphorus sources. The extent of growt...

journal_title：Applied and environmental microbiology

authors： Rosenberg A,Alexander M

更新日期：1979-05-01 00:00:00

abstract：:The metal-reducing bacterium Shewanella oneidensis MR-1 displays remarkable anaerobic respiratory plasticity, which is reflected in the extensive number of electron transport components encoded in its genome. In these studies, several cell components required for the reduction of vanadium(V) were determined. V(V) redu...

journal_title：Applied and environmental microbiology

authors： Myers JM,Antholine WE,Myers CR

更新日期：2004-03-01 00:00:00

abstract：UNLABELLED:The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological distribution among microorganisms. MAAs have a range of physiological functions, including protection against UV radiation, making them important from a biotechnological perspective. In the present study, we iden...

journal_title：Applied and environmental microbiology

authors： D'Agostino PM,Javalkote VS,Mazmouz R,Pickford R,Puranik PR,Neilan BA

更新日期：2016-09-16 00:00:00

abstract：:Recent studies have reported high levels of fecal indicator enterococci in marine beach sand. This study aimed to determine the spatial and temporal variation of enterococcal abundance and to evaluate its relationships with microbial community parameters in Hawaii beach sand and water. Sampling at 23 beaches on the Is...

journal_title：Applied and environmental microbiology

authors： Cui H,Yang K,Pagaling E,Yan T

更新日期：2013-06-01 00:00:00

abstract：:A mixed methanogenic culture fed with glucose was perturbed with butyrate and formate to investigate the role of formate in the acetogenesis of butyrate. A free energy analysis suggests that formate rather than H(2) was the interspecies electron carrier for butyrate conversion into acetate for the culture studied. ...

journal_title：Applied and environmental microbiology

authors： Bae J,McCarty PL

更新日期：1993-02-01 00:00:00