Association of Cry1Ac toxin resistance in Helicoverpa zea (Boddie) with increased alkaline phosphatase levels in the midgut lumen.

abstract：:We previously reported the isolation of a 21.5-kb genomic DNA fragment from Pseudomonas sp. strain ADP, which contains the atzA gene, encoding the first metabolic step for the degradation of the herbicide atrazine (M. de Souza, L. P. Wackett, K. L. Boundy-Mills, R. T. Mandelbaum, and M. J. Sadowsky, Appl. Environ. Mic...

journal_title：Applied and environmental microbiology

authors： Boundy-Mills KL,de Souza ML,Mandelbaum RT,Wackett LP,Sadowsky MJ

更新日期：1997-03-01 00:00:00

abstract：:Carbohydrate components (simple sugars and polysaccharides) of cell walls of pearl millet (Pennisetum americanum L., cv. Gahi) were studied as potential substrates for the root-associated diazotroph Azospirillum brasiliense Sp. 7. Simple sugars were utilized, but no evidence was obtained to support the suggestion that...

journal_title：Applied and environmental microbiology

authors： Myers ML,Hubbell DH

更新日期：1987-12-01 00:00:00

abstract：:We report a method which combines epifluorescence microscopy and microautoradiography to determine both the total number of microorganisms in natural water populations and those individual organisms active in the uptake of specific substrates. After incubation with H-labeled substrate, the sample is filtered and, whil...

journal_title：Applied and environmental microbiology

authors： Tabor PS,Neihof RA

更新日期：1982-10-01 00:00:00

abstract：:We developed a PCR detection method that selectively recognizes a single biological control agent and demonstrated that universally primed PCR (UP-PCR) can identify strain-specific markers. Antagonistic strains of Clonostachys rosea (syn. Gliocladium roseum) were screened by UP-PCR, and a strain-specific marker was id...

journal_title：Applied and environmental microbiology

authors： Bulat SA,Lübeck M,Alekhina IA,Jensen DF,Knudsen IM,Lübeck PS

更新日期：2000-11-01 00:00:00

abstract：:Antialgal allelochemicals were isolated from Phragmites communis Tris. The isolated allelopathic fraction showed strong inhibition activity on the growth of Chlorella pyrenoidosa and Microcystis aeruginosa but had no inhibition on Chlorella vulgaris. The 50% effective concentrations (EC50) of the allelopathic fraction...

journal_title：Applied and environmental microbiology

authors： Li FM,Hu HY

更新日期：2005-11-01 00:00:00

abstract：:The symbiotic association between the roseobacter Silicibacter sp. strain TM1040 and the dinoflagellate Pfiesteria piscicida involves bacterial chemotaxis to dinoflagellate-produced dimethylsulfoniopropionate (DMSP), DMSP demethylation, and ultimately a biofilm on the surface of the host. Biofilm formation is coincide...

journal_title：Applied and environmental microbiology

authors： Geng H,Bruhn JB,Nielsen KF,Gram L,Belas R

更新日期：2008-03-01 00:00:00

abstract：:A total of 221 strains of Lactobacillus isolated from meat and meat products were screened for antagonistic activities under conditions that eliminated the effects of organic acids and hydrogen peroxide. Nineteen strains of Lactobacillus sake, three strains of Lactobacillus plantarum, and one strain of Lactobacillus c...

journal_title：Applied and environmental microbiology

authors： Schillinger U,Lücke FK

更新日期：1989-08-01 00:00:00

abstract：:Several cell surface characteristics of bacteriophage-resistant Lactococcus lactis subsp. cremoris SK110 were compared with those of its phage-sensitive derivative SK112. After centrifugation, SK110 cells resisted suspension more strongly than SK112 cells. SK112 was more negatively charged and had a more hydrophobic c...

journal_title：Applied and environmental microbiology

authors： Sijtsma L,Jansen N,Hazeleger WC,Wouters JT,Hellingwerf KJ

更新日期：1990-10-01 00:00:00

abstract：:Nitrogen-fixing symbiosis between bacteria and the tree legume mesquite (Prosopis glandulosa) is important for the maintenance of many desert ecosystems. Genes essential for nodulation and for extending the host range to mesquite were isolated from cosmid libraries of Rhizobium (mesquite) sp. strain HW17b and Bradyrhi...

journal_title：Applied and environmental microbiology

authors： Thomas PM,Golly KF,Virginia RA,Zyskind JW

更新日期：1995-09-01 00:00:00

abstract：:Cyanase catalyzes the decomposition of cyanate into CO(2) and ammonium, with carbamate as an unstable intermediate. The cyanase of Pseudomonas pseudoalcaligenes CECT5344 was negatively regulated by ammonium and positively regulated by cyanate, cyanide, and some cyanometallic complexes. Cyanase activity was not detecte...

journal_title：Applied and environmental microbiology

authors： Luque-Almagro VM,Huertas MJ,Sáez LP,Luque-Romero MM,Moreno-Vivián C,Castillo F,Roldán MD,Blasco R

更新日期：2008-10-01 00:00:00

abstract：:Protoplasts of Claviceps purpurea were prepared by treatment of mycelium with a lytic mixture of snail gut enzyme and cellulase from Trichoderma viride. Such protoplasts could be efficiently lysed by Triton X-100 treatment at high osmotic pressure without Ca or Mg, allowing the release of intact vacuoles in high yield...

journal_title：Applied and environmental microbiology

authors： Keller U,Madry N,Kleinkauf H,Glund K

更新日期：1984-04-01 00:00:00

abstract：:The most common source of Salmonella infections in humans is food of poultry origin. Salmonella enterica serovar Enteritidis has a particular affinity for the contamination of the egg supply. In this study, the medium-chain fatty acids (MCFA), caproic, caprylic, and capric acid, were evaluated for the control of Salmo...

journal_title：Applied and environmental microbiology

authors： Van Immerseel F,De Buck J,Boyen F,Bohez L,Pasmans F,Volf J,Sevcik M,Rychlik I,Haesebrouck F,Ducatelle R

更新日期：2004-06-01 00:00:00

abstract：:A toluene-degrading sulfate-reducing bacterium, strain Tol2, was isolated from marine sediment under strictly anoxic conditions. Toluene was toxic if applied directly to the medium at concentrations higher than 0.5 mM. To provide toluene continuously at a nontoxic concentration, it was supplied in an inert hydrophobic...

journal_title：Applied and environmental microbiology

authors： Rabus R,Nordhaus R,Ludwig W,Widdel F

更新日期：1993-05-01 00:00:00

abstract：:Antibodies against a microcystin (MCYST) leucine-arginine variant (MCYST-LR) were demonstrated 4 weeks after immunization of rabbits with either MCYST-LR-polylysine- or MCYST-LR-ethylenediamine-modified bovine serum albumin. A radioimmunoassay (RIA), a direct competitive enzyme-linked immunosorbent assay (ELISA), and ...

journal_title：Applied and environmental microbiology

authors： Chu FS,Huang X,Wei RD,Carmichael WW

更新日期：1989-08-01 00:00:00

abstract：:Pure cultures of methylotrophs and methanotrophs are known to oxidize methyl bromide (MeBr); however, their ability to oxidize tropospheric concentrations (parts per trillion by volume [pptv]) has not been tested. Methylotrophs and methanotrophs were able to consume MeBr provided at levels that mimicked the tropospher...

journal_title：Applied and environmental microbiology

authors： Goodwin KD,Varner RK,Crill PM,Oremland RS

更新日期：2001-12-01 00:00:00

abstract：:Bacteriophages are the source of many valuable tools for molecular biology and genetic manipulation. In Streptomyces, most DNA cloning vectors are based on serine integrase site-specific DNA recombination systems derived from phage. Because of their efficiency and simplicity, serine integrases are also used for divers...

journal_title：Applied and environmental microbiology

authors： Fogg PCM,Haley JA,Stark WM,Smith MCM

更新日期：2017-02-15 00:00:00

abstract：:Lantibiotics, such as nisin and subtilin, are lanthionine-containing peptides that exhibit antimicrobial as well as pheromone-like autoinducing activity. Autoinduction is specific for each lantibiotic, and reporter systems for nisin and subtilin autoinduction are available. In this report, we used the previously repor...

journal_title：Applied and environmental microbiology

authors： Fuchs SW,Jaskolla TW,Bochmann S,Kötter P,Wichelhaus T,Karas M,Stein T,Entian KD

更新日期：2011-03-01 00:00:00

abstract：:Florfenicol resistance was analyzed in 230 enteric pig isolates collected between 1998 and 2006. PCR, plasmid profiling, Southern blot hybridization, and a mixed-broth conjugation assay suggested the intra- and interspecies plasmid-mediated transfer of florfenicol resistance among the isolates that exhibited MICs for ...

journal_title：Applied and environmental microbiology

authors： Rayamajhi N,Cha SB,Kang ML,Lee SI,Lee HS,Yoo HS

更新日期：2009-09-01 00:00:00

abstract：:Because of high diurnal water quality fluctuations in raw municipal wastewater, the use of proportional autosampling over a period of 24 h at municipal wastewater treatment plants (WWTPs) to evaluate carbon, nitrogen, and phosphorus removal has become a standard in many countries. Microbial removal or load estimation ...

journal_title：Applied and environmental microbiology

authors： Mayer RE,Vierheilig J,Egle L,Reischer GH,Saracevic E,Mach RL,Kirschner AK,Zessner M,Sommer R,Farnleitner AH

更新日期：2015-08-01 00:00:00

abstract：:Staphylococcus aureus is a common hospital and household pathogen. Given the emergence of antibiotic-resistant derivatives of this pathogen resulting from the use of antibiotics as general treatment, development of alternative therapeutic strategies is urgently needed. Here, we assess the feasibility of killing S. aur...

journal_title：Applied and environmental microbiology

authors： Chen C,Chen L,Yi Y,Chen C,Wu LF,Song T

更新日期：2016-02-12 00:00:00

abstract：:Crenarchaeol, a membrane-spanning glycerol dialkyl glycerol tetraether (GDGT) containing a cyclohexane moiety in addition to four cyclopentane moieties, was originally hypothesized to be synthesized exclusively by the mesophilic Crenarchaeota. Recent studies reporting the occurrence of crenarchaeol in hot springs and ...

journal_title：Applied and environmental microbiology

authors： Pitcher A,Schouten S,Sinninghe Damsté JS

更新日期：2009-07-01 00:00:00

abstract：:Bifidobacterium and Lactobacillus can beneficially affect the host by producing acetic acid and lactic acid, which lower pH and thereby inhibit the growth of pathogens or allow the probiotic bacteria to compete with pathogens for epithelial adhesion sites and nutrients. The transmural migration of enteric organisms in...

journal_title：Applied and environmental microbiology

authors： Wang IK,Lai HC,Yu CJ,Liang CC,Chang CT,Kuo HL,Yang YF,Lin CC,Lin HH,Liu YL,Chang YC,Wu YY,Chen CH,Li CY,Chuang FR,Huang CC,Lin CH,Lin HC

更新日期：2012-02-01 00:00:00

abstract：:Escherichia coli O157:H7 is a leading cause of food-borne illness. This human pathogen produces Shiga toxins (Stx1 and Stx2) which inhibit protein synthesis by inactivating ribosome function. The present study describes a novel cell-based assay to detect Stx2 and inhibitors of toxin activity. A Vero cell line harborin...

journal_title：Applied and environmental microbiology

authors： Quiñones B,Massey S,Friedman M,Swimley MS,Teter K

更新日期：2009-03-01 00:00:00

abstract：UNLABELLED:In the summer of 2010, Vibrio parahaemolyticus caused an outbreak in Maryland linked to the consumption of oysters. Strains isolated from both stool and oyster samples were indistinguishable by pulsed-field gel electrophoresis (PFGE). However, the oysters contained other potentially pathogenic V. parahaemoly...

journal_title：Applied and environmental microbiology

authors： Haendiges J,Jones J,Myers RA,Mitchell CS,Butler E,Toro M,Gonzalez-Escalona N

更新日期：2016-05-16 00:00:00

abstract：:The oxidation of fluorene, a polycyclic hydrocarbon which is not a substrate for fungal lignin peroxidase, was studied in liquid cultures of Phanerochaete chrysosporium and in vitro with P. chrysosporium extracellular enzymes. Intact fungal cultures metabolized fluorene to 9-hydroxyfluorene via 9-fluorenone. Some conv...

journal_title：Applied and environmental microbiology

authors： Bogan BW,Lamar RT,Hammel KE

更新日期：1996-05-01 00:00:00

abstract：:A number of chemical, microbial, and eukaryotic indicators have been proposed as indicators of fecal pollution sources in water bodies. No single one of the indicators tested to date has been able to determine the source of fecal pollution in water. However, the combined use of different indicators has been demonstrat...

journal_title：Applied and environmental microbiology

authors： Ballesté E,Bonjoch X,Belanche LA,Blanch AR

更新日期：2010-03-01 00:00:00

abstract：:The aim of this work was to investigate the medium requirements for growth and production of exopolysaccharides by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2772. The strain was grown in batch cultures on a chemically defined medium, and the technique of single omission of medium components was applied to deter...

journal_title：Applied and environmental microbiology

authors： Grobben GJ,Chin-Joe I,Kitzen VA,Boels IC,Boer F,Sikkema J,Smith MR,de Bont JA

更新日期：1998-04-01 00:00:00

abstract：:An investigation of the Hg2+ resistance mechanism of four freshwater and four coastal marine bacteria that did not hybridize with a mer operonic probe was conducted (T. Barkay, C. Liebert, and M. Gillman, Appl. Environ. Microbiol. 55:1196-1202, 1989). Hybridization with a merA probe, the gene encoding the mercuric red...

journal_title：Applied and environmental microbiology

authors： Barkay T,Gillman M,Liebert C

更新日期：1990-06-01 00:00:00

abstract：:Epilithic periphyton communities were sampled at three sites on the Minnesota shoreline of Lake Superior from June 2004 to August 2005 to determine if fecal coliforms and Escherichia coli were present throughout the ice-free season. Fecal coliform densities increased up to 4 orders of magnitude in early summer, reache...

journal_title：Applied and environmental microbiology

authors： Ksoll WB,Ishii S,Sadowsky MJ,Hicks RE

更新日期：2007-06-01 00:00:00

abstract：:We have cloned the homoserine dehydrogenase genes (hom) from the gram-negative obligate methylotrophs Methylobacillus glycogenes ATCC 21276 and ATCC 21371 by complementation of an Escherichia coli homoserine dehydrogenase-deficient mutant. The 4.15-kb DNA fragment cloned from M. glycogenes ATCC 21371 also complemented...

journal_title：Applied and environmental microbiology

authors： Motoyama H,Maki K,Anazawa H,Ishino S,Teshiba S

更新日期：1994-01-01 00:00:00