Production and characterization of antibodies against microcystins.

Abstract:

:Antibodies against a microcystin (MCYST) leucine-arginine variant (MCYST-LR) were demonstrated 4 weeks after immunization of rabbits with either MCYST-LR-polylysine- or MCYST-LR-ethylenediamine-modified bovine serum albumin. A radioimmunoassay (RIA), a direct competitive enzyme-linked immunosorbent assay (ELISA), and an indirect competitive ELISA were developed for characterization of the antibodies. Indirect ELISA and RIA revealed that MCYST-LR-ethylenediamine-bovine serum albumin was a better immunogen. Competitive RIA and direct ELISA revealed that the antibodies had good cross-reactivities with an MCYST-arginine-arginine variant (MCYST-RR), MCYST-LR, an MCYST-tyrosine-arginine variant (MCYST-YR), and nodularin (NODLN); but they had lower reactivities with variants MCYST-leucine-tyrosine (MCYST-LY) and MCYST-leucine-alanine (MCYST-LA). The antibodies did not cross-react with ozonolyzed MCYST-LR. The concentrations causing 50% inhibition of binding of reduced MCYST-LR to the antibodies by MCYST-RR, MCYST-LR, MCYST-YR, NODLN, MCYST-LA, and MCYST-LY in the RIA were 43, 105, 112, 503, 671, and 1,920 ng/ml, respectively. The concentrations causing 50% inhibition of binding of MCYST-LR-horseradish peroxidase to the antibodies by MCYST-RR, MCYST-LR, MCYST-YR, NODLN, MCYST-LY, and MCYST-LA in the ELISA were 1.75, 2.2, 3.4, 4.6, 50, and 114 ng/ml, respectively.

journal_name

Appl Environ Microbiol

authors

Chu FS,Huang X,Wei RD,Carmichael WW

doi

10.1128/AEM.55.8.1928-1933.1989

subject

Has Abstract

pub_date

1989-08-01 00:00:00

pages

1928-33

issue

8

eissn

0099-2240

issn

1098-5336

journal_volume

55

pub_type

杂志文章
  • Detection and characterization of cyanobacterial nifH genes.

    abstract::The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594. Results confirmed that the DNA sequence of the 359-bp...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.3.880-887.1994

    authors: Ben-Porath J,Zehr JP

    更新日期:1994-03-01 00:00:00

  • Biogeochemical Conditions Favoring Magnetite Formation during Anaerobic Iron Reduction.

    abstract::Several anaerobic bacteria isolated from the sediments of Contrary Creek, an iron-rich environment, produced magnetite when cultured in combinations but not when cultured alone in synthetic iron oxyhydroxide medium. When glucose was added as a carbon source, the pH of the medium decreased (to 5.5) and no magnetite was...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.11.2610-2616.1987

    authors: Bell PE,Mills AL,Herman JS

    更新日期:1987-11-01 00:00:00

  • Evidence for iron-dependent nitrate respiration in the dissimilatory iron-reducing bacterium Geobacter metallireducens.

    abstract::The dissimilatory iron-reducing bacterium Geobacter metallireducens was found to require iron at a concentration in excess of 50 microM for continuous cultivation on nitrate. Growth yield (approximately 3-fold), cytochrome c content (approximately 7-fold), and nitrate (approximately 4.5-fold) and nitrite (approximatel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.8.3750-3752.2001

    authors: Senko JM,Stolz JF

    更新日期:2001-08-01 00:00:00

  • Extensive manipulation of caseicins A and B highlights the tolerance of these antimicrobial peptides to change.

    abstract::Caseicins A and B are low-molecular-weight antimicrobial peptides which are released by proteolytic digestion of sodium caseinate. Caseicin A (IKHQGLPQE) is a nine-amino-acid cationic peptide, and caseicin B (VLNENLLR) is a neutral eight-amino-acid peptide; both have previously been shown to exhibit antibacterial acti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07312-11

    authors: Norberg S,O'Connor PM,Stanton C,Ross RP,Hill C,Fitzgerald GF,Cotter PD

    更新日期:2012-04-01 00:00:00

  • Biocide-Tolerant Listeria monocytogenes Isolates from German Food Production Plants Do Not Show Cross-Resistance to Clinically Relevant Antibiotics.

    abstract::Contamination of food during processing is recognized as a main transmission route of Listeria monocytogenes To prevent microbial contamination, biocides are widely applied as disinfectants in food processing plants. However, there are concerns about the development of antimicrobial resistance in foodborne pathogens d...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01253-19

    authors: Roedel A,Dieckmann R,Brendebach H,Hammerl JA,Kleta S,Noll M,Al Dahouk S,Vincze S

    更新日期:2019-10-01 00:00:00

  • Phylogenetic position and in situ identification of ectosymbiotic spirochetes on protists in the termite gut.

    abstract::Phylogenetic relationships, diversity, and in situ identification of spirochetes in the gut of the termite Neotermes koshunensis were examined without cultivation, with an emphasis on ectosymbionts attached to flagellated protists. Spirochetes in the gut microbial community investigated so far are related to the genus...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.1.625-633.2003

    authors: Noda S,Ohkuma M,Yamada A,Hongoh Y,Kudo T

    更新日期:2003-01-01 00:00:00

  • Differences between Pseudomonas syringae pv. syringae B728a and Pantoea agglomerans BRT98 in epiphytic and endophytic colonization of leaves.

    abstract::The leaf colonization strategies of two bacterial strains were investigated. The foliar pathogen Pseudomonas syringae pv. syringae strain B728a and the nonpathogen Pantoea agglomerans strain BRT98 were marked with a green fluorescent protein, and surface (epiphytic) and subsurface (endophytic) sites of bean and maize ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.2.1220-1228.2003

    authors: Sabaratnam S,Beattie GA

    更新日期:2003-02-01 00:00:00

  • High-Level Nickel Resistance in Alcaligenes xylosoxydans 31A and Alcaligenes eutrophus KTO2.

    abstract::Two new nickel-resistant strains of Alcaligenes species were selected from a large number (about 400) of strains isolated from ecosystems polluted by heavy metals and were studied on the physiological and molecular level. Alcaligenes xylosoxydans 31A is a heterotrophic bacterium, and Alcaligenes eutrophus KTO2 is an a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.11.3301-3309.1991

    authors: Schmidt T,Stoppel RD,Schlegel HG

    更新日期:1991-11-01 00:00:00

  • Chloroflexi CL500-11 Populations That Predominate Deep-Lake Hypolimnion Bacterioplankton Rely on Nitrogen-Rich Dissolved Organic Matter Metabolism and C1 Compound Oxidation.

    abstract::The Chloroflexi CL500-11 clade contributes a large proportion of the bacterial biomass in the oxygenated hypolimnia of deep lakes worldwide, including the world's largest freshwater system, the Laurentian Great Lakes. Traits that allow CL500-11 to thrive and its biogeochemical role in these environments are currently ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03014-15

    authors: Denef VJ,Mueller RS,Chiang E,Liebig JR,Vanderploeg HA

    更新日期:2015-12-18 00:00:00

  • Evaluation of staphylococcal cell surface display and flow cytometry for postselectional characterization of affinity proteins in combinatorial protein engineering applications.

    abstract::For efficient generation of high-affinity protein-based binding molecules, fast and reliable downstream characterization platforms are needed. In this work, we have explored the use of staphylococcal cell surface display together with flow cytometry for affinity characterization of candidate affibody molecules directl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01432-07

    authors: Löfblom J,Sandberg J,Wernérus H,Ståhl S

    更新日期:2007-11-01 00:00:00

  • Novel surface display system for proteins on non-genetically modified gram-positive bacteria.

    abstract::A novel display system is described that allows highly efficient immobilization of heterologous proteins on bacterial surfaces in applications for which the use of genetically modified bacteria is less desirable. This system is based on nonliving and non-genetically modified gram-positive bacterial cells, designated g...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.1.880-889.2006

    authors: Bosma T,Kanninga R,Neef J,Audouy SA,van Roosmalen ML,Steen A,Buist G,Kok J,Kuipers OP,Robillard G,Leenhouts K

    更新日期:2006-01-01 00:00:00

  • Identification of a third msa gene in Renibacterium salmoninarum and the associated virulence phenotype.

    abstract::Renibacterium salmoninarum, a gram-positive diplococcobacillus, causes bacterial kidney disease, a condition that can result in extensive morbidity and mortality among stocks of fish. An immunodominant extracellular protein, called major soluble antigen (MSA), is encoded by two identical genes, msa1 and msa2. We found...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.11.6488-6494.2004

    authors: Rhodes LD,Coady AM,Deinhard RK

    更新日期:2004-11-01 00:00:00

  • Pulsed-electric field treatment enhances the bactericidal action of nisin against Bacillus cereus.

    abstract::Vegetative cells of Bacillus cereus were subjected to low doses of nisin (0.06 microg/ml) and mild pulsed-electric field treatment (16.7 kV/cm, 50 pulses each of 2-micros duration). Combining both treatments resulted in a reduction of 1.8 log units more than the sum of the reductions obtained with the single treatment...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.1.428-430.2000

    authors: Pol IE,Mastwijk HC,Bartels PV,Smid EJ

    更新日期:2000-01-01 00:00:00

  • Isolation and characterization of Linocin M18, a bacteriocin produced by Brevibacterium linens.

    abstract::Brevibacterium linens M18, isolated from red smear cheese, produces a substance that inhibits the growth of Listeria spp. and several coryneform and other gram-positive bacteria. No gram-negative bacteria were inhibited. The substance is heat labile, sensitive to proteolytic enzymes, and stable between pH 3 and 12. Hi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.10.3809-3814.1994

    authors: Valdés-Stauber N,Scherer S

    更新日期:1994-10-01 00:00:00

  • Comparison of the efficacies of disinfectants to control microbial contamination in dental unit water systems in general dental practices across the European Union.

    abstract::Water delivered by dental unit water systems (DUWS) in general dental practices can harbor high numbers of bacteria, including opportunistic pathogens. Biofilms on tubing within DUWS provide a reservoir for microorganisms and should be controlled. This study compared disinfection products for their ability to meet the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.2.1380-1387.2006

    authors: Schel AJ,Marsh PD,Bradshaw DJ,Finney M,Fulford MR,Frandsen E,Østergaard E,ten Cate JM,Moorer WR,Mavridou A,Kamma JJ,Mandilara G,Stösser L,Kneist S,Araujo R,Contreras N,Goroncy-Bermes P,O'Mullane D,Burke F,O'Reilly P

    更新日期:2006-02-01 00:00:00

  • Thymidine Incorporation by the Microbial Community of Standing Dead Spartina alterniflora.

    abstract::Thymidine incorporation by the microbial community on standing dead leaves of Spartina alterniflora did not obey many of the assumptions inherent in the use of the technique in planktonic systems. Incorporation rates of [methly-H]thymidine were nonsaturable over a wide concentration range (10 to 10 nM). Owing to metab...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.5.1206-1208.1986

    authors: Fallon RD,Newell SY

    更新日期:1986-11-01 00:00:00

  • Distribution of cryV-type insecticidal protein genes in Bacillus thuringiensis and cloning of cryV-type genes from Bacillus thuringiensis subsp. kurstaki and Bacillus thuringiensis subsp. entomocidus.

    abstract::DNA dot blot hybridizations with a cryV-specific probe and a cryI-specific probe were performed to screen 24 Bacillus thuringiensis strains for their cryV-type (lepidopteran- and coleopteran-specific) and cryI-type (lepidopteran-specific) insecticidal crystal protein gene contents, respectively. The cryV-specific prob...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.6.2402-2407.1995

    authors: Shin BS,Park SH,Choi SK,Koo BT,Lee ST,Kim JI

    更新日期:1995-06-01 00:00:00

  • Molecular diversity and characterization of tetracycline-resistant Staphylococcus aureus isolates from a poultry processing plant.

    abstract::DNA fingerprinting and molecular characterization showed that the tetracycline-resistant Staphylococcus aureus population of a South African poultry processing plant comprised one or possibly several tet(K)-containing endemic clones that contaminated chicken and machinery surfaces at all sampled processing stages. The...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.1.574-579.2005

    authors: Huys G,D'Haene K,Van Eldere J,von Holy A,Swings J

    更新日期:2005-01-01 00:00:00

  • Possible role of streptomycin released from spore cell wall of Streptomyces griseus.

    abstract::Vegetative mycelia and spores of the investigated high- and low-producer strains of Streptomyces griseus bound significant amounts (4%) of streptomycin, which could be removed by increasing ionic strength. The release of antibiotic from the spores was easier when the spores were germinating. This phenomenon is conside...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.2.438-440.1985

    authors: Szabó I,Benedek A,Barabás G

    更新日期:1985-08-01 00:00:00

  • Bacteriophage ecology in Escherichia coli and Pseudomonas aeruginosa mixed-biofilm communities.

    abstract::Phage therapy is being reexamined as a strategy for bacterial control in medical and other environments. As microorganisms often live in mixed populations, we examined the effect of Escherichia coli bacteriophage λW60 and Pseudomonas aeruginosa bacteriophage PB-1 infection on the viability of monoculture and mixed-spe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01797-10

    authors: Kay MK,Erwin TC,McLean RJ,Aron GM

    更新日期:2011-02-01 00:00:00

  • Evidence for Multidrug Resistance in Nonpathogenic Mycoplasma Species Isolated from South African Poultry.

    abstract::One hundred seventy-eight mycoplasma strains isolated from South African poultry flocks between 2003 and 2015 were identified by full-genome sequencing and phylogenetic analysis of the 16S rRNA gene and were classified as follows: Mycoplasma gallisepticum (25%), M. gallinarum (25%), M. gallinaceum, (23%), M. pullorum ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01660-18

    authors: Beylefeld A,Wambulawaye P,Bwala DG,Gouws JJ,Lukhele OM,Wandrag DBR,Abolnik C

    更新日期:2018-10-17 00:00:00

  • Competition for light between toxic and nontoxic strains of the harmful cyanobacterium Microcystis.

    abstract::The cyanobacterium Microcystis can produce microcystins, a family of toxins that are of major concern in water management. In several lakes, the average microcystin content per cell gradually declines from high levels at the onset of Microcystis blooms to low levels at the height of the bloom. Such seasonal dynamics m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02892-06

    authors: Kardinaal WE,Tonk L,Janse I,Hol S,Slot P,Huisman J,Visser PM

    更新日期:2007-05-01 00:00:00

  • Shifts in identity and activity of methanotrophs in arctic lake sediments in response to temperature changes.

    abstract::Methane (CH(4)) flux to the atmosphere is mitigated via microbial CH(4) oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH(4) emissions. We used DNA-based...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00853-12

    authors: He R,Wooller MJ,Pohlman JW,Quensen J,Tiedje JM,Leigh MB

    更新日期:2012-07-01 00:00:00

  • Prevalence of Ca²⁺-ATPase-mediated carbonate dissolution among cyanobacterial euendoliths.

    abstract::Recent physiological work has shown that the filamentous euendolithic cyanobacterium Mastigocoleus testarum (strain BC008) is able to bore into solid carbonates using Ca²⁺-ATPases to take up Ca²⁺ from the medium at the excavation front, promoting dissolution of CaCO₃ there. It is not known, however, if this is a wides...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06633-11

    authors: Ramírez-Reinat EL,Garcia-Pichel F

    更新日期:2012-01-01 00:00:00

  • Imidacloprid Decreases Honey Bee Survival Rates but Does Not Affect the Gut Microbiome.

    abstract::Accumulating evidence suggests that pesticides have played a role in the increased rate of honey bee colony loss. One of the most commonly used pesticides in the United States is the neonicotinoid imidacloprid. Although the primary mode of action of imidacloprid is on the insect nervous system, it has also been shown ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00545-18

    authors: Raymann K,Motta EVS,Girard C,Riddington IM,Dinser JA,Moran NA

    更新日期:2018-06-18 00:00:00

  • Phylogenetic analysis of polyketide synthase I domains from soil metagenomic libraries allows selection of promising clones.

    abstract::The metagenomic approach provides direct access to diverse unexplored genomes, especially from uncultivated bacteria in a given environment. This diversity can conceal many new biosynthetic pathways. Type I polyketide synthases (PKSI) are modular enzymes involved in the biosynthesis of many natural products of industr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.9.5522-5527.2004

    authors: Ginolhac A,Jarrin C,Gillet B,Robe P,Pujic P,Tuphile K,Bertrand H,Vogel TM,Perrière G,Simonet P,Nalin R

    更新日期:2004-09-01 00:00:00

  • Optimum pH levels for eluting enteroviruses from sludge solids with beef extract.

    abstract::This study demonstrates that elution of enteroviruses from a mixture of primary- and activated-sludge solids with beef extract at pH 9.2 +/- 0.2 may be less efficient than elution with beef extract at pH 7.2 +/- 0.2 and that elution of enteroviruses from extended-aeration-sludge solids with beef extract is at best no ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.7.1880-1881.1988

    authors: Berg G,Sullivan G

    更新日期:1988-07-01 00:00:00

  • Expanded Fermi solution for estimating the survival of ingested pathogenic and probiotic microbial cells and spores.

    abstract::The expanded Fermi solution was originally developed for estimating the number of food-poisoning victims when information concerning the circumstances of exposure is scarce. The method has been modified for estimating the initial number of pathogenic or probiotic cells or spores so that enough of them will survive the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01448-10

    authors: Peleg M,Normand MD,Horowitz J,Corradini MG

    更新日期:2011-01-01 00:00:00

  • Antifungal metabolites (monorden, monocillin IV, and cerebrosides) from Humicola fuscoatra traaen NRRL 22980, a mycoparasite of Aspergillus flavus sclerotia.

    abstract::The mycoparasite Humicola fuscoatra NRRL 22980 was isolated from a sclerotium of Aspergillus flavus that had been buried in a cornfield near Tifton, Ga. When grown on autoclaved rice, this fungus produced the antifungal metabolites monorden, monocillin IV, and a new monorden analog. Each metabolite produced a clear zo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.11.4482-4484.1998

    authors: Wicklow DT,Joshi BK,Gamble WR,Gloer JB,Dowd PF

    更新日期:1998-11-01 00:00:00

  • Effects of Agricultural Management on Rhizosphere Microbial Structure and Function in Processing Tomato Plants.

    abstract::Agricultural management practices affect bulk soil microbial communities and the functions they carry out, but it remains unclear how these effects extend to the rhizosphere in different agroecosystem contexts. Given close linkages between rhizosphere processes and plant nutrition and productivity, understanding how m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01064-19

    authors: Schmidt JE,Vannette RL,Igwe A,Blundell R,Casteel CL,Gaudin ACM

    更新日期:2019-08-01 00:00:00